Click here to switch to the map view.

The map label for this gene is rpoE [H]

Identifier: 239618509

GI number: 239618509

Start: 2279830

End: 2280390

Strand: Reverse

Name: rpoE [H]

Synonym: Kole_2150

Alternate gene names: 239618509

Gene position: 2280390-2279830 (Counterclockwise)

Preceding gene: 239618512

Following gene: 239618508

Centisome position: 99.06

GC content: 43.14

Gene sequence:

>561_bases
TTGGACGAGAAAAAATTGATCGAAGGACTGAAAAAGAAAGAAATGTGGGCTTATGAGGTACTGTACGACAAATACGCTCC
TCGATTGGGGAGTGTTATTCGTTCCTACCTCGGCACAGATGATGTTGATGACGTCCTTCAGGAGACTTTTATAAAGGTGT
TTAAAAATATAAAAAAGTTCCGTGGCGAAGCGAAACTATCTACATGGCTTTATAGGGTCGCAATCAACGTGTGTAATGAC
ATTATTGCAAAACGGAAGAGAAACCGGGAGTACTTGACGGACTTCCAGGAAAACGACGAGAAACCATTTCTCGAACCTCC
AGCGCCCAACGATGTGTTCAGGGAAGTAATGAACGAGCTGAGCTATGAAGAACTGACAAATATAATCGCAAAACTCCCTG
AAGAAGATAGGTTGTTGATAAAGCTTAGAGATATTGAGGGGCTTTCATATGAAGAGATAGCCCAGATTCTCGGGAAGCCA
AACGGAACAATAAAGAGCCGATTACACTATGCCCGGAAACGTCTCAGGCATCTCCTTGAGGAGGGAGGTTACAATGAGTA
A

Upstream 100 bases:

>100_bases
CCCGGTAATATTCTATCAGAGGATAAGAATTCTTTATAGTATAATTAGTATGAACCGTAACAGCAAAAGTGGTGTCAAAA
TTCATGACAGGGGGATTCCC

Downstream 100 bases:

>100_bases
TTTTGACAGATTCCTTGACGGTGAAATTCCAAAAGAAGCTCTGAATGAAAAAGATAAAGAAGCCGTGGTGAAATATGTTA
GAGCACTTTTGGTCTTTAAA

Product: RNA polymerase, sigma-24 subunit, ECF subfamily

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MDEKKLIEGLKKKEMWAYEVLYDKYAPRLGSVIRSYLGTDDVDDVLQETFIKVFKNIKKFRGEAKLSTWLYRVAINVCND
IIAKRKRNREYLTDFQENDEKPFLEPPAPNDVFREVMNELSYEELTNIIAKLPEEDRLLIKLRDIEGLSYEEIAQILGKP
NGTIKSRLHYARKRLRHLLEEGGYNE

Sequences:

>Translated_186_residues
MDEKKLIEGLKKKEMWAYEVLYDKYAPRLGSVIRSYLGTDDVDDVLQETFIKVFKNIKKFRGEAKLSTWLYRVAINVCND
IIAKRKRNREYLTDFQENDEKPFLEPPAPNDVFREVMNELSYEELTNIIAKLPEEDRLLIKLRDIEGLSYEEIAQILGKP
NGTIKSRLHYARKRLRHLLEEGGYNE
>Mature_186_residues
MDEKKLIEGLKKKEMWAYEVLYDKYAPRLGSVIRSYLGTDDVDDVLQETFIKVFKNIKKFRGEAKLSTWLYRVAINVCND
IIAKRKRNREYLTDFQENDEKPFLEPPAPNDVFREVMNELSYEELTNIIAKLPEEDRLLIKLRDIEGLSYEEIAQILGKP
NGTIKSRLHYARKRLRHLLEEGGYNE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=182, Percent_Identity=33.5164835164835, Blast_Score=106, Evalue=8e-25,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21974; Mature: 21974

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEKKLIEGLKKKEMWAYEVLYDKYAPRLGSVIRSYLGTDDVDDVLQETFIKVFKNIKKF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RGEAKLSTWLYRVAINVCNDIIAKRKRNREYLTDFQENDEKPFLEPPAPNDVFREVMNEL
HCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
SYEELTNIIAKLPEEDRLLIKLRDIEGLSYEEIAQILGKPNGTIKSRLHYARKRLRHLLE
CHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EGGYNE
HCCCCC
>Mature Secondary Structure
MDEKKLIEGLKKKEMWAYEVLYDKYAPRLGSVIRSYLGTDDVDDVLQETFIKVFKNIKKF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RGEAKLSTWLYRVAINVCNDIIAKRKRNREYLTDFQENDEKPFLEPPAPNDVFREVMNEL
HCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
SYEELTNIIAKLPEEDRLLIKLRDIEGLSYEEIAQILGKPNGTIKSRLHYARKRLRHLLE
CHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EGGYNE
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]