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The map label for this gene is pheT [H]

Identifier: 239618504

GI number: 239618504

Start: 2274441

End: 2276801

Strand: Reverse

Name: pheT [H]

Synonym: Kole_2145

Alternate gene names: 239618504

Gene position: 2276801-2274441 (Counterclockwise)

Preceding gene: 239618505

Following gene: 239618503

Centisome position: 98.9

GC content: 43.67

Gene sequence:

>2361_bases
ATGAGAATACCCGTCGAATGGTTGAATGAGATAATAGATCTATCAGATAAAGATGTTCAGGAGTTAACCAGTGCGCTTTC
ACTCAGCGGTACAGCGGTTGAAGGTGTTGAATATCCCTGGGATTATCTTGAAGGAGCGGTAACGGGAAAAATTGAAAAGG
TTATCCCCCATCCGAATGCAGATAAGCTCGTTGTCACCGAGGTGAATATAGGGGACAAACTTTTAACGGTAGTTACTGCT
GATCTCAGCGTCAAACCTGAAGAATACGTGGCTGTGCTTCCTGAAGGTGGCAGGCTATGGGATCTGGAGATAAGAAAGAG
GAACTTTCGCGGAGTGATGTCTGAAGGGATGTTCCTTTCATTTGAAGAGTTAAGGCTCGAGGAAAAATCAACACACATCC
TCAGATTCTCCGAAGCGGTACCACTGGGAAAAGACGTTAAAGATATCCTGAAACTGAACTATCCTGTAATAGAGGTGGAG
TTGACACCCAATAGAGGTGATTGTCTGTCAATGATCGGTATTGCGCGAGAGTTGCGGGCTATATACAAACGTCCTCTCAA
AATCCCGGAGATTAACGAAGTCAAAGGAACAGATAAACCGGTAGAAATAAGAATTGAAGATGAGGGATGTCAAAGATACA
CAGCACTTGTGATGAAAAACGTCAGGGTTAAAGAATCACCACTGTGGTTGAAGAGACGCCTCATCGCTGCAGGATTGAGA
CCCTTAAACAACGTTGTTGACATCACCAACTATGTCTTATTAGAAACTGGCCACCCGGTTCACGCTTTTGATCTGGCCAA
AATCCCGAACAAAAAAATCGTGGTTAGGAATGCTTCGCAGGGAGAAAAAATGACACTGCTTGATGGTAAGGTTATCGAGC
TTCTTGACACGGATCTGCTGATAACGGATGGAGAAACACCGCTTGCGCTGGCCGGTATTATGGGAGGAGCGAATTCGGGG
ATCTCAGAAGAGACCACCGATGTCCTATTAGAGGTAGCAGTCTTTGATCCTGTCAGAATAAGAAAAACGGCCAGGAGATT
GGGTATATCTACTGATAGCTCTTACCGCTTCGAAAGAGGGATAGACGCAAAGGATTCGCTGTATGTCATCAAAAGGCTTG
CCACCCTGTTGCAACAACTCGCTTCTGCCGAGATAGCCAGCGGAATAATCGACGTTGGGCAATCTTCAGAACCAGGAACC
ATTCATCTGCGAAAATGGTTTGTGGATAAGGTTCTTGGAACTGTGGTTCCTATAAAAGACGTAGAAGAAATATTGCTATC
TCTAGGATTTTCGCTCAAAGAATCCGGTGATGGCTGGAATGTTACCCCACCATCTCACAGGCACGATATCGATCAGGAGA
TCGATCTTGTGGAAGAAATCGGCCGTATCTATGGTTACGACAAGATTGAAAGCGAGCTTCCGCGAATTACTCCAGGTGGC
TGCTCACAATCTTCAGAGGTAAAAACCATAAAAAGGGTAAAAAATATCATGACTTCCCTGGGATATGACGAAGTTATCAC
ATACAGTTTTATTAATCCGGATAGTATCACCTCGCTGGACAAGAATCTCAAATACACCTTACTGAAAAATCCTCTTTCAA
TCGAGATGAGTGCGCTAAGACCCTCTCTCCTTTTTGGGCTTCTTGAGGTGGCTTCATACAACTATCGCAGGCAAAATAGA
GATCTCAAAATATTTGAAGTGGGAAAGATTTTCTCGGTGGATCCAAAAAAGCCTGAATCGCTGTCTCTCGGATTTTTGGC
CATGGGGAAAGAGAACAAGGAGGATTACACAGACAAACGCATGGTTTCTTTCTACACCTTCAAAGGAGTTGTTGAAGATG
TCCTTGAATCTTACGGCGTTTCCGTTACCTTCAAAGAACCAGAAGAGAGCTGGCTGGAAAAACGCGCTTCTGCGGATATT
TATATAGGCGAAAAGAAAATAGGCTTTTTCGGAGTCTTCGATAGCAGTCTAGCTGATAGGCTCTACGAAATAAAGAACGC
TGAGGTCTTCATCGCTGAACTCGACCTTGAAAGCCTACTGGAAAAAGAAACGAAAAGGTGTATTAATGTAAATATGTCTC
CGTTCCCGAGGGTATTCAGAGATCTTTCGATCCTCATAAGCCCTGAAATCACCTATGAAAAAATAAAAGAGACAATTCAG
AAAGCCGGCAGCAAATATCTGACGGATATCAGGGTTACCGACATATATCGTGGAAAAGGAATCCCCAAGGGATTTAGGAG
CATTACAATCTCTCTTACCTTCGAGTCTTTTACAGAAACGCTGAAGGAAGATGAGATCAATTCAGCGATAAACAATATTA
TTAGCTCCCTGGAAGAAATTGGAGTGAAGTTACGTGGATAA

Upstream 100 bases:

>100_bases
GTGTCGAAAGGATCGCGATGCTCAAATACAACCTCGGGGATATCAGAGACCTTCTCAGAAATGATCGAAGATTCCTCAAA
AAGTTCATGGGGGTGAATGC

Downstream 100 bases:

>100_bases
TGGTTCTCTGGAGAAAATCGTTGACGAAGTTAAAAAGAAGTTGTACGAAATATGGCGGGAAAAGTTGTTTTCCCGCCGTT
TCAAAGTCGACAGAAAATCC

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 786; Mature: 786

Protein sequence:

>786_residues
MRIPVEWLNEIIDLSDKDVQELTSALSLSGTAVEGVEYPWDYLEGAVTGKIEKVIPHPNADKLVVTEVNIGDKLLTVVTA
DLSVKPEEYVAVLPEGGRLWDLEIRKRNFRGVMSEGMFLSFEELRLEEKSTHILRFSEAVPLGKDVKDILKLNYPVIEVE
LTPNRGDCLSMIGIARELRAIYKRPLKIPEINEVKGTDKPVEIRIEDEGCQRYTALVMKNVRVKESPLWLKRRLIAAGLR
PLNNVVDITNYVLLETGHPVHAFDLAKIPNKKIVVRNASQGEKMTLLDGKVIELLDTDLLITDGETPLALAGIMGGANSG
ISEETTDVLLEVAVFDPVRIRKTARRLGISTDSSYRFERGIDAKDSLYVIKRLATLLQQLASAEIASGIIDVGQSSEPGT
IHLRKWFVDKVLGTVVPIKDVEEILLSLGFSLKESGDGWNVTPPSHRHDIDQEIDLVEEIGRIYGYDKIESELPRITPGG
CSQSSEVKTIKRVKNIMTSLGYDEVITYSFINPDSITSLDKNLKYTLLKNPLSIEMSALRPSLLFGLLEVASYNYRRQNR
DLKIFEVGKIFSVDPKKPESLSLGFLAMGKENKEDYTDKRMVSFYTFKGVVEDVLESYGVSVTFKEPEESWLEKRASADI
YIGEKKIGFFGVFDSSLADRLYEIKNAEVFIAELDLESLLEKETKRCINVNMSPFPRVFRDLSILISPEITYEKIKETIQ
KAGSKYLTDIRVTDIYRGKGIPKGFRSITISLTFESFTETLKEDEINSAINNIISSLEEIGVKLRG

Sequences:

>Translated_786_residues
MRIPVEWLNEIIDLSDKDVQELTSALSLSGTAVEGVEYPWDYLEGAVTGKIEKVIPHPNADKLVVTEVNIGDKLLTVVTA
DLSVKPEEYVAVLPEGGRLWDLEIRKRNFRGVMSEGMFLSFEELRLEEKSTHILRFSEAVPLGKDVKDILKLNYPVIEVE
LTPNRGDCLSMIGIARELRAIYKRPLKIPEINEVKGTDKPVEIRIEDEGCQRYTALVMKNVRVKESPLWLKRRLIAAGLR
PLNNVVDITNYVLLETGHPVHAFDLAKIPNKKIVVRNASQGEKMTLLDGKVIELLDTDLLITDGETPLALAGIMGGANSG
ISEETTDVLLEVAVFDPVRIRKTARRLGISTDSSYRFERGIDAKDSLYVIKRLATLLQQLASAEIASGIIDVGQSSEPGT
IHLRKWFVDKVLGTVVPIKDVEEILLSLGFSLKESGDGWNVTPPSHRHDIDQEIDLVEEIGRIYGYDKIESELPRITPGG
CSQSSEVKTIKRVKNIMTSLGYDEVITYSFINPDSITSLDKNLKYTLLKNPLSIEMSALRPSLLFGLLEVASYNYRRQNR
DLKIFEVGKIFSVDPKKPESLSLGFLAMGKENKEDYTDKRMVSFYTFKGVVEDVLESYGVSVTFKEPEESWLEKRASADI
YIGEKKIGFFGVFDSSLADRLYEIKNAEVFIAELDLESLLEKETKRCINVNMSPFPRVFRDLSILISPEITYEKIKETIQ
KAGSKYLTDIRVTDIYRGKGIPKGFRSITISLTFESFTETLKEDEINSAINNIISSLEEIGVKLRG
>Mature_786_residues
MRIPVEWLNEIIDLSDKDVQELTSALSLSGTAVEGVEYPWDYLEGAVTGKIEKVIPHPNADKLVVTEVNIGDKLLTVVTA
DLSVKPEEYVAVLPEGGRLWDLEIRKRNFRGVMSEGMFLSFEELRLEEKSTHILRFSEAVPLGKDVKDILKLNYPVIEVE
LTPNRGDCLSMIGIARELRAIYKRPLKIPEINEVKGTDKPVEIRIEDEGCQRYTALVMKNVRVKESPLWLKRRLIAAGLR
PLNNVVDITNYVLLETGHPVHAFDLAKIPNKKIVVRNASQGEKMTLLDGKVIELLDTDLLITDGETPLALAGIMGGANSG
ISEETTDVLLEVAVFDPVRIRKTARRLGISTDSSYRFERGIDAKDSLYVIKRLATLLQQLASAEIASGIIDVGQSSEPGT
IHLRKWFVDKVLGTVVPIKDVEEILLSLGFSLKESGDGWNVTPPSHRHDIDQEIDLVEEIGRIYGYDKIESELPRITPGG
CSQSSEVKTIKRVKNIMTSLGYDEVITYSFINPDSITSLDKNLKYTLLKNPLSIEMSALRPSLLFGLLEVASYNYRRQNR
DLKIFEVGKIFSVDPKKPESLSLGFLAMGKENKEDYTDKRMVSFYTFKGVVEDVLESYGVSVTFKEPEESWLEKRASADI
YIGEKKIGFFGVFDSSLADRLYEIKNAEVFIAELDLESLLEKETKRCINVNMSPFPRVFRDLSILISPEITYEKIKETIQ
KAGSKYLTDIRVTDIYRGKGIPKGFRSITISLTFESFTETLKEDEINSAINNIISSLEEIGVKLRG

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=789, Percent_Identity=36.8821292775665, Blast_Score=454, Evalue=1e-128,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 88312; Mature: 88312

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPVEWLNEIIDLSDKDVQELTSALSLSGTAVEGVEYPWDYLEGAVTGKIEKVIPHPNA
CCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCEECCCCCCHHHHCCCCCCCHHHHCCCCCC
DKLVVTEVNIGDKLLTVVTADLSVKPEEYVAVLPEGGRLWDLEIRKRNFRGVMSEGMFLS
CEEEEEEECCCCEEEEEEEECCCCCCCCEEEEECCCCCEEEEEEHHCHHHHHHCCCCEEE
FEELRLEEKSTHILRFSEAVPLGKDVKDILKLNYPVIEVELTPNRGDCLSMIGIARELRA
HHHHHHHHCCCEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
IYKRPLKIPEINEVKGTDKPVEIRIEDEGCQRYTALVMKNVRVKESPLWLKRRLIAAGLR
HHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
PLNNVVDITNYVLLETGHPVHAFDLAKIPNKKIVVRNASQGEKMTLLDGKVIELLDTDLL
HHHHHHHHHCEEEEECCCCEEEEEHHHCCCCEEEEEECCCCCEEEEECCHHHEEECCCEE
ITDGETPLALAGIMGGANSGISEETTDVLLEVAVFDPVRIRKTARRLGISTDSSYRFERG
EECCCCCEEEHEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHCC
IDAKDSLYVIKRLATLLQQLASAEIASGIIDVGQSSEPGTIHLRKWFVDKVLGTVVPIKD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCCHHH
VEEILLSLGFSLKESGDGWNVTPPSHRHDIDQEIDLVEEIGRIYGYDKIESELPRITPGG
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCC
CSQSSEVKTIKRVKNIMTSLGYDEVITYSFINPDSITSLDKNLKYTLLKNPLSIEMSALR
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCEEEEECCCCEEEHHHHH
PSLLFGLLEVASYNYRRQNRDLKIFEVGKIFSVDPKKPESLSLGFLAMGKENKEDYTDKR
HHHHHHHHHHHHCCHHHCCCCEEEEEECCEEECCCCCCCCCEEEEEEECCCCCCCHHHHH
MVSFYTFKGVVEDVLESYGVSVTFKEPEESWLEKRASADIYIGEKKIGFFGVFDSSLADR
HHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCEEECCCCCEEEEEECHHHHHH
LYEIKNAEVFIAELDLESLLEKETKRCINVNMSPFPRVFRDLSILISPEITYEKIKETIQ
HHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEECCCCCHHHHHHHHH
KAGSKYLTDIRVTDIYRGKGIPKGFRSITISLTFESFTETLKEDEINSAINNIISSLEEI
HCCCHHCCEEEEEEEECCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVKLRG
CCEECC
>Mature Secondary Structure
MRIPVEWLNEIIDLSDKDVQELTSALSLSGTAVEGVEYPWDYLEGAVTGKIEKVIPHPNA
CCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCEECCCCCCHHHHCCCCCCCHHHHCCCCCC
DKLVVTEVNIGDKLLTVVTADLSVKPEEYVAVLPEGGRLWDLEIRKRNFRGVMSEGMFLS
CEEEEEEECCCCEEEEEEEECCCCCCCCEEEEECCCCCEEEEEEHHCHHHHHHCCCCEEE
FEELRLEEKSTHILRFSEAVPLGKDVKDILKLNYPVIEVELTPNRGDCLSMIGIARELRA
HHHHHHHHCCCEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
IYKRPLKIPEINEVKGTDKPVEIRIEDEGCQRYTALVMKNVRVKESPLWLKRRLIAAGLR
HHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
PLNNVVDITNYVLLETGHPVHAFDLAKIPNKKIVVRNASQGEKMTLLDGKVIELLDTDLL
HHHHHHHHHCEEEEECCCCEEEEEHHHCCCCEEEEEECCCCCEEEEECCHHHEEECCCEE
ITDGETPLALAGIMGGANSGISEETTDVLLEVAVFDPVRIRKTARRLGISTDSSYRFERG
EECCCCCEEEHEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHCC
IDAKDSLYVIKRLATLLQQLASAEIASGIIDVGQSSEPGTIHLRKWFVDKVLGTVVPIKD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCCHHH
VEEILLSLGFSLKESGDGWNVTPPSHRHDIDQEIDLVEEIGRIYGYDKIESELPRITPGG
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCC
CSQSSEVKTIKRVKNIMTSLGYDEVITYSFINPDSITSLDKNLKYTLLKNPLSIEMSALR
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCEEEEECCCCEEEHHHHH
PSLLFGLLEVASYNYRRQNRDLKIFEVGKIFSVDPKKPESLSLGFLAMGKENKEDYTDKR
HHHHHHHHHHHHCCHHHCCCCEEEEEECCEEECCCCCCCCCEEEEEEECCCCCCCHHHHH
MVSFYTFKGVVEDVLESYGVSVTFKEPEESWLEKRASADIYIGEKKIGFFGVFDSSLADR
HHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCEEECCCCCEEEEEECHHHHHH
LYEIKNAEVFIAELDLESLLEKETKRCINVNMSPFPRVFRDLSILISPEITYEKIKETIQ
HHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEECCCCCHHHHHHHHH
KAGSKYLTDIRVTDIYRGKGIPKGFRSITISLTFESFTETLKEDEINSAINNIISSLEEI
HCCCHHCCEEEEEEEECCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVKLRG
CCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]