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The map label for this gene is pheS [H]

Identifier: 239618505

GI number: 239618505

Start: 2276798

End: 2277835

Strand: Reverse

Name: pheS [H]

Synonym: Kole_2146

Alternate gene names: 239618505

Gene position: 2277835-2276798 (Counterclockwise)

Preceding gene: 239618506

Following gene: 239618504

Centisome position: 98.94

GC content: 46.82

Gene sequence:

>1038_bases
ATGGTGTCAGATATCAGAGAATTTGATTTAGAAAAGGCCTTAAAAGAGATTTCTGAAGCTACGAACTCAGCCGAGCTTCA
GGTAATCAAAGCTCGCTATTTAGGAAAACAAGGGCTCGTGACTTCCATGCTTAAAAAGATTCGGGAACTCCCTCCAGAGG
AGCGAAAGGGCTACGGGAAGATGGTTAACCAGTTCAAAAGGACCCTTGAAGAGAAAATCTCTGAAAAACGAAGAGTTCTC
GAAATGGAAGCGCGAAAAATCAGGGAACTTGAGGACAGGTACGATGTTTCCTTGCCTGGAGCTAGACGCGACAGAGGTGC
ATTACATCTTCTCACGCAGGTTATGGAAGAGGTTCAGGAAATCTGGAGCAGCATGGGATTTGAGGTTGTGCTTGGTCCGG
AAATTGAGGAGGTTTACTACAACTTCGAAGCATTGAATACTCCGGAATGGCATCCTGCAAGAGATATGCATGATACTTTT
TACCTCGATGAACGTTGGCTCCTCAGAACCCATACCTCTCCAGTTCAAATAAGGGTTATGGAGTCGCGAAAGCCGCCTCT
GGCGATCTTTTCACCCGGGAAATGTTACCGCAAGGATACCCCGGACAGTACTCATCTGCCAATGTTCCATCAATTTGAGG
TTTTGTATGTCGATAGGCACGTCTCAGTGGCGCATTTGAAAGGTGTTCTTGAGGTTTTCGTGAAAAGGATGTTTGGTGAA
AACAGAAAAATCCGATTGGGACCAAGTTTCTTCCCCTTTACTGAACCGAGCTTTGAGGTTGACGTGTCTTGCGGAATTTG
TGGTGGTACCGGTTGCCGTAGCTGCGGTGGTACCGGCTGGCTGGAAATCCTCGGATCAGGAATGGTTCATCCCAATGTTT
TCAGAAATGTTGGTTACGATCCCGAGATCTGGTCTGGATTCGCACTCGGTACGGGTGTCGAAAGGATCGCGATGCTCAAA
TACAACCTCGGGGATATCAGAGACCTTCTCAGAAATGATCGAAGATTCCTCAAAAAGTTCATGGGGGTGAATGCATGA

Upstream 100 bases:

>100_bases
AAGTTAAACAAATCAATATCTCTTTTTTTGGTATATGTAAACGCTGTGCACAACAACAAACATCGTGAAGATAAACAACT
TGCCGTTTCTGGGGGTGCTT

Downstream 100 bases:

>100_bases
GAATACCCGTCGAATGGTTGAATGAGATAATAGATCTATCAGATAAAGATGTTCAGGAGTTAACCAGTGCGCTTTCACTC
AGCGGTACAGCGGTTGAAGG

Product: phenylalanyl-tRNA synthetase, alpha subunit

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MVSDIREFDLEKALKEISEATNSAELQVIKARYLGKQGLVTSMLKKIRELPPEERKGYGKMVNQFKRTLEEKISEKRRVL
EMEARKIRELEDRYDVSLPGARRDRGALHLLTQVMEEVQEIWSSMGFEVVLGPEIEEVYYNFEALNTPEWHPARDMHDTF
YLDERWLLRTHTSPVQIRVMESRKPPLAIFSPGKCYRKDTPDSTHLPMFHQFEVLYVDRHVSVAHLKGVLEVFVKRMFGE
NRKIRLGPSFFPFTEPSFEVDVSCGICGGTGCRSCGGTGWLEILGSGMVHPNVFRNVGYDPEIWSGFALGTGVERIAMLK
YNLGDIRDLLRNDRRFLKKFMGVNA

Sequences:

>Translated_345_residues
MVSDIREFDLEKALKEISEATNSAELQVIKARYLGKQGLVTSMLKKIRELPPEERKGYGKMVNQFKRTLEEKISEKRRVL
EMEARKIRELEDRYDVSLPGARRDRGALHLLTQVMEEVQEIWSSMGFEVVLGPEIEEVYYNFEALNTPEWHPARDMHDTF
YLDERWLLRTHTSPVQIRVMESRKPPLAIFSPGKCYRKDTPDSTHLPMFHQFEVLYVDRHVSVAHLKGVLEVFVKRMFGE
NRKIRLGPSFFPFTEPSFEVDVSCGICGGTGCRSCGGTGWLEILGSGMVHPNVFRNVGYDPEIWSGFALGTGVERIAMLK
YNLGDIRDLLRNDRRFLKKFMGVNA
>Mature_345_residues
MVSDIREFDLEKALKEISEATNSAELQVIKARYLGKQGLVTSMLKKIRELPPEERKGYGKMVNQFKRTLEEKISEKRRVL
EMEARKIRELEDRYDVSLPGARRDRGALHLLTQVMEEVQEIWSSMGFEVVLGPEIEEVYYNFEALNTPEWHPARDMHDTF
YLDERWLLRTHTSPVQIRVMESRKPPLAIFSPGKCYRKDTPDSTHLPMFHQFEVLYVDRHVSVAHLKGVLEVFVKRMFGE
NRKIRLGPSFFPFTEPSFEVDVSCGICGGTGCRSCGGTGWLEILGSGMVHPNVFRNVGYDPEIWSGFALGTGVERIAMLK
YNLGDIRDLLRNDRRFLKKFMGVNA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=282, Percent_Identity=31.5602836879433, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI5729820, Length=242, Percent_Identity=30.1652892561983, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1788007, Length=331, Percent_Identity=47.4320241691843, Blast_Score=320, Evalue=6e-89,
Organism=Caenorhabditis elegans, GI32563657, Length=282, Percent_Identity=30.1418439716312, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17508957, Length=282, Percent_Identity=30.1418439716312, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32566635, Length=324, Percent_Identity=29.320987654321, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=281, Percent_Identity=29.5373665480427, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6325304, Length=279, Percent_Identity=25.8064516129032, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18858079, Length=283, Percent_Identity=30.0353356890459, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17137424, Length=251, Percent_Identity=27.0916334661355, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39810; Mature: 39810

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSDIREFDLEKALKEISEATNSAELQVIKARYLGKQGLVTSMLKKIRELPPEERKGYGK
CCCCHHHHHHHHHHHHHHHHCCCHHHEEHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHH
MVNQFKRTLEEKISEKRRVLEMEARKIRELEDRYDVSLPGARRDRGALHLLTQVMEEVQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
IWSSMGFEVVLGPEIEEVYYNFEALNTPEWHPARDMHDTFYLDERWLLRTHTSPVQIRVM
HHHHCCCEEEECCCHHHHHCCHHHCCCCCCCCCCCCCCCEEECCCCEEEECCCCEEEEEE
ESRKPPLAIFSPGKCYRKDTPDSTHLPMFHQFEVLYVDRHVSVAHLKGVLEVFVKRMFGE
CCCCCCEEEECCCCCCCCCCCCCCCCCHHHCEEEEEEECCCHHHHHHHHHHHHHHHHHCC
NRKIRLGPSFFPFTEPSFEVDVSCGICGGTGCRSCGGTGWLEILGSGMVHPNVFRNVGYD
CCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHCCCC
PEIWSGFALGTGVERIAMLKYNLGDIRDLLRNDRRFLKKFMGVNA
CCHHCCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVSDIREFDLEKALKEISEATNSAELQVIKARYLGKQGLVTSMLKKIRELPPEERKGYGK
CCCCHHHHHHHHHHHHHHHHCCCHHHEEHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHH
MVNQFKRTLEEKISEKRRVLEMEARKIRELEDRYDVSLPGARRDRGALHLLTQVMEEVQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
IWSSMGFEVVLGPEIEEVYYNFEALNTPEWHPARDMHDTFYLDERWLLRTHTSPVQIRVM
HHHHCCCEEEECCCHHHHHCCHHHCCCCCCCCCCCCCCCEEECCCCEEEECCCCEEEEEE
ESRKPPLAIFSPGKCYRKDTPDSTHLPMFHQFEVLYVDRHVSVAHLKGVLEVFVKRMFGE
CCCCCCEEEECCCCCCCCCCCCCCCCCHHHCEEEEEEECCCHHHHHHHHHHHHHHHHHCC
NRKIRLGPSFFPFTEPSFEVDVSCGICGGTGCRSCGGTGWLEILGSGMVHPNVFRNVGYD
CCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHCCCC
PEIWSGFALGTGVERIAMLKYNLGDIRDLLRNDRRFLKKFMGVNA
CCHHCCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA