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The map label for this gene is folD

Identifier: 239618472

GI number: 239618472

Start: 2246009

End: 2246842

Strand: Reverse

Name: folD

Synonym: Kole_2113

Alternate gene names: 239618472

Gene position: 2246842-2246009 (Counterclockwise)

Preceding gene: 239618473

Following gene: 239618471

Centisome position: 97.6

GC content: 44.24

Gene sequence:

>834_bases
ATGGGTATACTTTTAGATGGTAAGCCAGTGGCAAAAATGATTTACGCGGAAATAAAAGAGTGGTTGGGTAATCTTCAGGA
GAAACCACCGAAGTTGGTATTGTTTTGCAGTGAACCGGATGACTCGACCAAAACCTATATGAACTCGATAGTCAAGCGCG
GTGGTAAACTGGGAATTTCGGTGGAGATTTGTCATGCAGGGGAAAATCCTGTTGAAGAGATCAAAAAGCTGAACGAATCC
AGAGATGTTGCGGGCGTTATGATAATGCACCCGCTTAAGAACGTTGACGAGAAACTGGTTGTCTCAGCTCTTTCACTGGA
AAAGGATGTAGAAGGAAGAACGCCGGGGAATCTAGGCGGTATCATGACCGGTGATGAGAGCTTTGCACCTCCCACTGCAG
AAGCGGTTATGGAGATACTTAGGTTTTACGATGTAAGTTTGAGTGGAAAAGACGTTACCATCGTTGGCCGTTCAACTACT
GTGGGAAAGCCGCTTTCGATGTTAATGCTCAAAAAAGGAATAGATGCCACCGTTACCATATGTCATTCGCGAACAAAAAA
CCTCGTGGAAAAGATAAAAAGAGCCAATGTGCTAGTTTCTGCAGTTGGACGCGCCGGATTCATAACGAAAGAAATGGTTG
GTAAAGATTCTATTATTATTGATGTGGGAATCAACCTCTATGACGGAAAAATAGTTGGAGACGTTGATTTTGAACAGGTT
GAACCTGAGGTAGCTGCAATAACTCCGGTTCCCGGAGGCGTAGGTATAGTAACTACGGCCATTCTTTTCAGACATTTCAT
GGTTTCTTCAAGGAGAATGGTCGGGAGGCGATGA

Upstream 100 bases:

>100_bases
GATATCATGCTGATGCCCGGCCTGGGAAAGGAATTTGGTGCTCAAAGGATCGACATTGACAAAAGCGGAAAGATTTCCGG
ACTATTCTGAGGGGTGTGAA

Downstream 100 bases:

>100_bases
GATGATTGAGTTTGATGAGGTTCCTCTTTACAAGAAAATAAAAGGGTATAGCTATCCTGAACTTGAGATACTGGCGGAAG
AATTCAGGAACTACATCGCT

Product: Methylenetetrahydrofolate dehydrogenase (NADP(+))

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MGILLDGKPVAKMIYAEIKEWLGNLQEKPPKLVLFCSEPDDSTKTYMNSIVKRGGKLGISVEICHAGENPVEEIKKLNES
RDVAGVMIMHPLKNVDEKLVVSALSLEKDVEGRTPGNLGGIMTGDESFAPPTAEAVMEILRFYDVSLSGKDVTIVGRSTT
VGKPLSMLMLKKGIDATVTICHSRTKNLVEKIKRANVLVSAVGRAGFITKEMVGKDSIIIDVGINLYDGKIVGDVDFEQV
EPEVAAITPVPGGVGIVTTAILFRHFMVSSRRMVGRR

Sequences:

>Translated_277_residues
MGILLDGKPVAKMIYAEIKEWLGNLQEKPPKLVLFCSEPDDSTKTYMNSIVKRGGKLGISVEICHAGENPVEEIKKLNES
RDVAGVMIMHPLKNVDEKLVVSALSLEKDVEGRTPGNLGGIMTGDESFAPPTAEAVMEILRFYDVSLSGKDVTIVGRSTT
VGKPLSMLMLKKGIDATVTICHSRTKNLVEKIKRANVLVSAVGRAGFITKEMVGKDSIIIDVGINLYDGKIVGDVDFEQV
EPEVAAITPVPGGVGIVTTAILFRHFMVSSRRMVGRR
>Mature_276_residues
GILLDGKPVAKMIYAEIKEWLGNLQEKPPKLVLFCSEPDDSTKTYMNSIVKRGGKLGISVEICHAGENPVEEIKKLNESR
DVAGVMIMHPLKNVDEKLVVSALSLEKDVEGRTPGNLGGIMTGDESFAPPTAEAVMEILRFYDVSLSGKDVTIVGRSTTV
GKPLSMLMLKKGIDATVTICHSRTKNLVEKIKRANVLVSAVGRAGFITKEMVGKDSIIIDVGINLYDGKIVGDVDFEQVE
PEVAAITPVPGGVGIVTTAILFRHFMVSSRRMVGRR

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=297, Percent_Identity=32.996632996633, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=31.5068493150685, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI94721354, Length=299, Percent_Identity=32.1070234113712, Blast_Score=131, Evalue=5e-31,
Organism=Homo sapiens, GI36796743, Length=222, Percent_Identity=29.2792792792793, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1786741, Length=276, Percent_Identity=32.2463768115942, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17568735, Length=254, Percent_Identity=33.8582677165354, Blast_Score=124, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=31.1418685121107, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321643, Length=302, Percent_Identity=31.4569536423841, Blast_Score=132, Evalue=4e-32,
Organism=Drosophila melanogaster, GI17136816, Length=300, Percent_Identity=31.3333333333333, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI17136818, Length=300, Percent_Identity=31.3333333333333, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24645718, Length=293, Percent_Identity=30.3754266211604, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17137370, Length=293, Percent_Identity=30.3754266211604, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI62472483, Length=293, Percent_Identity=30.3754266211604, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45551871, Length=293, Percent_Identity=30.3754266211604, Blast_Score=123, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_KOSOT (C5CI88)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941794.1
- GeneID:   7968525
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_2113
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30021; Mature: 29889

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGILLDGKPVAKMIYAEIKEWLGNLQEKPPKLVLFCSEPDDSTKTYMNSIVKRGGKLGIS
CCEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEE
VEICHAGENPVEEIKKLNESRDVAGVMIMHPLKNVDEKLVVSALSLEKDVEGRTPGNLGG
EEEEECCCCHHHHHHHHCCCCCHHHHHEEHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCC
IMTGDESFAPPTAEAVMEILRFYDVSLSGKDVTIVGRSTTVGKPLSMLMLKKGIDATVTI
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEE
CHSRTKNLVEKIKRANVLVSAVGRAGFITKEMVGKDSIIIDVGINLYDGKIVGDVDFEQV
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEECCEEECCEEEECCCHHHH
EPEVAAITPVPGGVGIVTTAILFRHFMVSSRRMVGRR
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GILLDGKPVAKMIYAEIKEWLGNLQEKPPKLVLFCSEPDDSTKTYMNSIVKRGGKLGIS
CEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEE
VEICHAGENPVEEIKKLNESRDVAGVMIMHPLKNVDEKLVVSALSLEKDVEGRTPGNLGG
EEEEECCCCHHHHHHHHCCCCCHHHHHEEHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCC
IMTGDESFAPPTAEAVMEILRFYDVSLSGKDVTIVGRSTTVGKPLSMLMLKKGIDATVTI
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEE
CHSRTKNLVEKIKRANVLVSAVGRAGFITKEMVGKDSIIIDVGINLYDGKIVGDVDFEQV
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEECCEEECCEEEECCCHHHH
EPEVAAITPVPGGVGIVTTAILFRHFMVSSRRMVGRR
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA