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The map label for this gene is truB [H]

Identifier: 239618461

GI number: 239618461

Start: 2231493

End: 2232422

Strand: Reverse

Name: truB [H]

Synonym: Kole_2102

Alternate gene names: 239618461

Gene position: 2232422-2231493 (Counterclockwise)

Preceding gene: 239618462

Following gene: 239618460

Centisome position: 96.97

GC content: 41.72

Gene sequence:

>930_bases
ATGAATTTGAACGGATTTCTTTTCATCGATAAACCTTCCGGGATTACTTCGCATGATGTTGTCGATGAAATCCGCAAGAA
GTTTGGAATAAAAAAGGTTGGGCATTCCGGGACCCTTGATCCCTTTGCCAGTGGTCTCCTCATTGTTGGTGTTGGCAAAG
CTACCAGGCTTTTGGAATATATAAAAGGCATGGATAAGACATATGAGGTTGAAATGAAACTCGGTGTTATGACCGATACC
TTTGATTTTACTGGTAATCTTGTCGAAGAAAGGGAAAGTTCCCATATCACCGAGGAAATGATCAGAGATGCTATTGAAAG
CTTCACCGGGGAATACATGCAGGTGCCACCGGCATATTCAGCGAAAAAATATAAAGGTGAGCGACTTTACAGGCTCGCGA
GAAAGGGGAAAATCATAAACTTACCACCAAAGAAGGTCACGATACACGACATCTATGTAAAATCCTTCGACCCCGTCGAA
ATGGCCGTATCATTCGTTGTAAAGGTCTCAGCTGGAACCTACGTTCGTTCCCTTGTTATGGACATAGGTTATAAGCTCGG
ATGTGGAGCAACAACGACCTTTTTGCGAAGGCTGGCTATAGGAAAGTTTTCAGTTTCCGATGCTGTTAAACTCGATGAGG
TTGATATTAAAGACATAAAGCCTATGAACGAAATTGTCAGTTTTCTTCCAGCGATTGTCGTTTCAAGAGAACAGGCCGAG
AAAGTGTTAAACGGTGGTCAGGTATATTCCAATGGAGTTGTTGAAATAAAAGGTGTATTTCCTAAAGAAGCCGTGATAAG
GATTATAGATGAAGATCAGAATCTACTCGCTATCGCCAGATCCGAAAGGACAAGCAAATTCATTAAGACTCTCACAAAAA
AAGTATCAAAAGAAAGAGTCGCAACATTGGAGAAGGTATTCCGTGGGTAA

Upstream 100 bases:

>100_bases
AGTTGAGATTTCATGAAGACATTGGCATAGAAGCGAGTTTACGAATATCAAAAATACTTGAAGAAATCAAAAAACAAGAA
CAGGAAAAGGAAGAGAATGA

Downstream 100 bases:

>100_bases
AAAATTCACTGTTTGCATAGGTAACTTTGATGGTGTTCATCTCGGACATAGAAAAATAATGTCGGAGACTCTTAAGCTTT
CCAGAAAGCTTAATGTGTGC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNLNGFLFIDKPSGITSHDVVDEIRKKFGIKKVGHSGTLDPFASGLLIVGVGKATRLLEYIKGMDKTYEVEMKLGVMTDT
FDFTGNLVEERESSHITEEMIRDAIESFTGEYMQVPPAYSAKKYKGERLYRLARKGKIINLPPKKVTIHDIYVKSFDPVE
MAVSFVVKVSAGTYVRSLVMDIGYKLGCGATTTFLRRLAIGKFSVSDAVKLDEVDIKDIKPMNEIVSFLPAIVVSREQAE
KVLNGGQVYSNGVVEIKGVFPKEAVIRIIDEDQNLLAIARSERTSKFIKTLTKKVSKERVATLEKVFRG

Sequences:

>Translated_309_residues
MNLNGFLFIDKPSGITSHDVVDEIRKKFGIKKVGHSGTLDPFASGLLIVGVGKATRLLEYIKGMDKTYEVEMKLGVMTDT
FDFTGNLVEERESSHITEEMIRDAIESFTGEYMQVPPAYSAKKYKGERLYRLARKGKIINLPPKKVTIHDIYVKSFDPVE
MAVSFVVKVSAGTYVRSLVMDIGYKLGCGATTTFLRRLAIGKFSVSDAVKLDEVDIKDIKPMNEIVSFLPAIVVSREQAE
KVLNGGQVYSNGVVEIKGVFPKEAVIRIIDEDQNLLAIARSERTSKFIKTLTKKVSKERVATLEKVFRG
>Mature_309_residues
MNLNGFLFIDKPSGITSHDVVDEIRKKFGIKKVGHSGTLDPFASGLLIVGVGKATRLLEYIKGMDKTYEVEMKLGVMTDT
FDFTGNLVEERESSHITEEMIRDAIESFTGEYMQVPPAYSAKKYKGERLYRLARKGKIINLPPKKVTIHDIYVKSFDPVE
MAVSFVVKVSAGTYVRSLVMDIGYKLGCGATTTFLRRLAIGKFSVSDAVKLDEVDIKDIKPMNEIVSFLPAIVVSREQAE
KVLNGGQVYSNGVVEIKGVFPKEAVIRIIDEDQNLLAIARSERTSKFIKTLTKKVSKERVATLEKVFRG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=267, Percent_Identity=34.0823970037453, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI215599015, Length=204, Percent_Identity=32.843137254902, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4503337, Length=204, Percent_Identity=32.843137254902, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI7661556, Length=214, Percent_Identity=29.4392523364486, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI2367200, Length=286, Percent_Identity=31.8181818181818, Blast_Score=167, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17553978, Length=194, Percent_Identity=33.5051546391753, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324037, Length=191, Percent_Identity=32.4607329842932, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=205, Percent_Identity=32.1951219512195, Blast_Score=89, Evalue=7e-19,
Organism=Drosophila melanogaster, GI281364189, Length=205, Percent_Identity=30.7317073170732, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364187, Length=205, Percent_Identity=30.7317073170732, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364185, Length=205, Percent_Identity=30.7317073170732, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364183, Length=205, Percent_Identity=30.7317073170732, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI62471759, Length=205, Percent_Identity=30.7317073170732, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17975520, Length=205, Percent_Identity=30.7317073170732, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01472 PUA; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 34456; Mature: 34456

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNGFLFIDKPSGITSHDVVDEIRKKFGIKKVGHSGTLDPFASGLLIVGVGKATRLLEY
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCEECCCCCCCCHHHCCEEEEECCHHHHHHHH
IKGMDKTYEVEMKLGVMTDTFDFTGNLVEERESSHITEEMIRDAIESFTGEYMQVPPAYS
HHCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
AKKYKGERLYRLARKGKIINLPPKKVTIHDIYVKSFDPVEMAVSFVVKVSAGTYVRSLVM
CHHHHHHHHHHHHHCCCEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHH
DIGYKLGCGATTTFLRRLAIGKFSVSDAVKLDEVDIKDIKPMNEIVSFLPAIVVSREQAE
HCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCHHHCCCHHHHHHHHHHHHHHHHHHH
KVLNGGQVYSNGVVEIKGVFPKEAVIRIIDEDQNLLAIARSERTSKFIKTLTKKVSKERV
HHHCCCEEECCCCEEEEECCCHHHHHEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
ATLEKVFRG
HHHHHHHCC
>Mature Secondary Structure
MNLNGFLFIDKPSGITSHDVVDEIRKKFGIKKVGHSGTLDPFASGLLIVGVGKATRLLEY
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCEECCCCCCCCHHHCCEEEEECCHHHHHHHH
IKGMDKTYEVEMKLGVMTDTFDFTGNLVEERESSHITEEMIRDAIESFTGEYMQVPPAYS
HHCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
AKKYKGERLYRLARKGKIINLPPKKVTIHDIYVKSFDPVEMAVSFVVKVSAGTYVRSLVM
CHHHHHHHHHHHHHCCCEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHH
DIGYKLGCGATTTFLRRLAIGKFSVSDAVKLDEVDIKDIKPMNEIVSFLPAIVVSREQAE
HCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCHHHCCCHHHHHHHHHHHHHHHHHHH
KVLNGGQVYSNGVVEIKGVFPKEAVIRIIDEDQNLLAIARSERTSKFIKTLTKKVSKERV
HHHCCCEEECCCCEEEEECCCHHHHHEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
ATLEKVFRG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571; 12499554 [H]