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The map label for this gene is fumB [H]

Identifier: 239618452

GI number: 239618452

Start: 2225101

End: 2225586

Strand: Reverse

Name: fumB [H]

Synonym: Kole_2093

Alternate gene names: 239618452

Gene position: 2225586-2225101 (Counterclockwise)

Preceding gene: 239618453

Following gene: 239618451

Centisome position: 96.68

GC content: 41.77

Gene sequence:

>486_bases
TTGAAGATAAAGGAACTTAAGGTTGGAAGTGAAATTGAGTACACCGGCGAACTCATTGTTATGCGCGATGCCGCACAAAA
GAGAACAAAAACGATACTGGAAGGAAATCTTCCGCTTCCTTTTGATTTAGAAGGCAAGATCGTATTTTACGCCGGACCAG
CCAAAGCTCCCGAAGGAAGAAATATAGGTTCAATAGGACCCACAACTTCTTCGAGAATGGATTCATACCTTGAAATGCTT
CTGAATCTCGGGGTTATTGCAACCGTGGGAAAAGGTAGAAGAAATGATTTTGTGAGAGAACTGTGCAAAAAGTACTCAGC
AGTTTACTTTATTGCTCCCAGTGGTGCTGCTGCGGCGCTTTCGCAAAGAATTATTTCTTCTAAGATAATAGCCTTTCACG
ACCTTGGACCTGAGGCGGTTTACAGATTGAAAGTCAAAAATTTTCCTCTCATCGTTGCTATTGACAGCGATGGGAATTGT
TTATAA

Upstream 100 bases:

>100_bases
GCCTATTCCGTGAGTATCGAGTACTATCCCACGCACATAGCGACGCTGCCTGTTGCGGTTTCTGTAGATTGCTTCATTTG
CAGGAAAGGGAGGATTGTTT

Downstream 100 bases:

>100_bases
CTCTTAGATTCAGTTTCCAAGAAATGATATAATTAAAATCTTTAATATCGTAACCCGTGAATGATATTTAAATCTAATTA
ACTAATTTAAAGGAGGAATA

Product: hydro-lyase, Fe-S type, tartrate/fumarate subfamily, beta subunit

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MKIKELKVGSEIEYTGELIVMRDAAQKRTKTILEGNLPLPFDLEGKIVFYAGPAKAPEGRNIGSIGPTTSSRMDSYLEML
LNLGVIATVGKGRRNDFVRELCKKYSAVYFIAPSGAAAALSQRIISSKIIAFHDLGPEAVYRLKVKNFPLIVAIDSDGNC
L

Sequences:

>Translated_161_residues
MKIKELKVGSEIEYTGELIVMRDAAQKRTKTILEGNLPLPFDLEGKIVFYAGPAKAPEGRNIGSIGPTTSSRMDSYLEML
LNLGVIATVGKGRRNDFVRELCKKYSAVYFIAPSGAAAALSQRIISSKIIAFHDLGPEAVYRLKVKNFPLIVAIDSDGNC
L
>Mature_161_residues
MKIKELKVGSEIEYTGELIVMRDAAQKRTKTILEGNLPLPFDLEGKIVFYAGPAKAPEGRNIGSIGPTTSSRMDSYLEML
LNLGVIATVGKGRRNDFVRELCKKYSAVYFIAPSGAAAALSQRIISSKIIAFHDLGPEAVYRLKVKNFPLIVAIDSDGNC
L

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=160, Percent_Identity=35, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1787897, Length=160, Percent_Identity=35.625, Blast_Score=108, Evalue=1e-25,
Organism=Escherichia coli, GI1789443, Length=164, Percent_Identity=37.1951219512195, Blast_Score=107, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 17564; Mature: 17564

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKELKVGSEIEYTGELIVMRDAAQKRTKTILEGNLPLPFDLEGKIVFYAGPAKAPEGR
CCCCCCCCCCCEEECCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCC
NIGSIGPTTSSRMDSYLEMLLNLGVIATVGKGRRNDFVRELCKKYSAVYFIAPSGAAAAL
CCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCEEEEECCCCHHHHH
SQRIISSKIIAFHDLGPEAVYRLKVKNFPLIVAIDSDGNCL
HHHHHHHHEEEEECCCHHHEEEEEECCCCEEEEECCCCCCC
>Mature Secondary Structure
MKIKELKVGSEIEYTGELIVMRDAAQKRTKTILEGNLPLPFDLEGKIVFYAGPAKAPEGR
CCCCCCCCCCCEEECCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCC
NIGSIGPTTSSRMDSYLEMLLNLGVIATVGKGRRNDFVRELCKKYSAVYFIAPSGAAAAL
CCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCEEEEECCCCHHHHH
SQRIISSKIIAFHDLGPEAVYRLKVKNFPLIVAIDSDGNCL
HHHHHHHHEEEEECCCHHHEEEEEECCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]