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The map label for this gene is thrS

Identifier: 239618448

GI number: 239618448

Start: 2219735

End: 2221681

Strand: Reverse

Name: thrS

Synonym: Kole_2089

Alternate gene names: 239618448

Gene position: 2221681-2219735 (Counterclockwise)

Preceding gene: 239618449

Following gene: 239618447

Centisome position: 96.51

GC content: 43.86

Gene sequence:

>1947_bases
ATGGGTTGTAAGATAGAATTGCCAGATGGCTCAATTAAAGAATACGATCATCCAGTAACTGCAGCGGATGTTGCCGCTGA
CCTGTCGCCGGCGTTAGCAAAAAGAGCAATTGGTGTTGAAGTCAACGGCGAGCTGTGGGATCTGACGAGAGAACTTCCAG
AGGAGTGTAAAATTAATCTGATACTCGAAAAGGACCCGAGAGCGCCTGAGTTTTTCCGCCATACCTTTTCACACATCCTG
GCTCAGGCGGTCATGAGAATCTACGGATCTGATAAAGTAAAACTGGGAATCGGTCCGGTTATTGAGAATGGTTTTTACTA
CGACTTTGATCTTGGTGATATCAGGCTGACGGAAGAGGATCTGCCAAAGATTGAAGAAGAGATGGAAAAAATCATAAAGG
AAGACTTAAAACTCGAGCGTTTTGAGCTTCCAAAAGAAGAAGCGATAAAGTTAATGAAAGATCAGAACCAGCCCTATAAA
GTGGAGCTCATTGAGGATCTCGAATCGGACGTAGCCACCTTTTACAGACAGGGTGAATTTGTGGATCTGTGTCGCGGTCC
GCATATGCCCTCTACTGGAAAAGTAAAGTACTTCAAGCTTCTGTCCGTTTCCGGAGCTTACTGGCGTGGAGATGAAAGAA
ATAAGATGCTCCAAAGGATTTACGGGACCGCCTTTGCCAAAAAATCCGAACTCGAAGCATACCTTAAGATGATGGAAGAA
GCCAAAAAGAGGGATCATCGCAAAATTGGACCTCAGCTTGGCCTTTTTAGTTTTAGTTATGAATACGCTCCTGGAATGCC
TATCTATCATCCTAAGGGAACCATCGTGCTAAACGAACTTATGAAATTCTCAAGGGAAGTGCATAGTAAGCATGGTTATC
AGGAGGTTATGACTCCGCAGATTATGAACGATGTCCTGTGGCATAGATCGGGACACTGGGACCATTACCGCGATAACATG
TATTTTACGGAGAAGGATGAGCAGCAATACGCTATTAAACCCATGAACTGTCCGGGACATATATTAATCTATAAATCCCG
AGCCATGAGCTACAGGGATCTGCCGTTGAAACTCTTTGAGTTTGGTAAGGTTCACCGTTATGAGAGAAGCGGTGTTTTGC
ACGGTCTCCTTAGAGTAAGGGGATTTATCCAGGATGACGCACACATTTTCTGTACCAGAGAACAATTAAAGGAACAGTTG
AAAGACGTCATTCGGTTTGTCGATATGATCTACACTCCTTTCGGGTTTGAATATAGAGCCGACTTATGTACCCGTCCAGA
AGATTTTATGGGCGATGTTGAAATATGGGATCTGGCAACGGAAGCTTTGAAAGATGCCATCGAATCTATCGGGTTGGAAT
ATAAAGTTAACGAAGGGGACGGAGCTTTTTACGGACCGAAGATCGATTTTCATGTCACGGATTCTATCGGGAGAGAGTGG
CAGTGTGCCACGATTCAGCTAGATTTCATGATGCCCGAGCGCTTTGAACTCACTTACGTTGGGCCAGACAATAAAGAATA
CAGGCCTGTTATGATTCATCGCGCTATTTACGGCTCTCTGGATAGATTTTTCGGTATTCTCATAGAACATTATGCCGGGG
CTTTTCCAACCTGGCTTGCTCCGGTACAAGTTGTCGCTATCCCAATTTCCGATAAACACGTTGACTACGCGAGAGAGGTA
GCAGATGCCCTTGCCGCAGAAGGCGTACGTGTTGATGTCGATGAAAAACAAAAATCCACAAACTACAAAATTCGTGAAGC
TCAATTAAATAAGATTCCTTACATGTTGATCCTTGGGGATAAAGAGAAGGAAAGCGAAACCATAACCGTGCGCTTGAGAA
CGGGTAAGAATATTTATGGGATGAAACTTGCGGAATTCAGTGCTAAGCTAAAAGAAGAAATATCGAACAGGATGAGTGAA
TCTATATTTAAATTTAAGGCAGACTGA

Upstream 100 bases:

>100_bases
CTGCAAGGGCAGTGGTAGAAGTCTCAAATCTTCCCAAGAATGCAATATTGGAGATTGAAGCAATAGCTAGGATTAGTAAT
AGTTAATAGGAGGTAATAAT

Downstream 100 bases:

>100_bases
TTGGCCATGTCCCTGCGAGAGCAGGGACTTCTTTTAAAATACAGGCTATAAGCAGCTCATTTCGGGCGTTATTAACGTTC
CTTGCTTTTGTCATCCTTTG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 648; Mature: 647

Protein sequence:

>648_residues
MGCKIELPDGSIKEYDHPVTAADVAADLSPALAKRAIGVEVNGELWDLTRELPEECKINLILEKDPRAPEFFRHTFSHIL
AQAVMRIYGSDKVKLGIGPVIENGFYYDFDLGDIRLTEEDLPKIEEEMEKIIKEDLKLERFELPKEEAIKLMKDQNQPYK
VELIEDLESDVATFYRQGEFVDLCRGPHMPSTGKVKYFKLLSVSGAYWRGDERNKMLQRIYGTAFAKKSELEAYLKMMEE
AKKRDHRKIGPQLGLFSFSYEYAPGMPIYHPKGTIVLNELMKFSREVHSKHGYQEVMTPQIMNDVLWHRSGHWDHYRDNM
YFTEKDEQQYAIKPMNCPGHILIYKSRAMSYRDLPLKLFEFGKVHRYERSGVLHGLLRVRGFIQDDAHIFCTREQLKEQL
KDVIRFVDMIYTPFGFEYRADLCTRPEDFMGDVEIWDLATEALKDAIESIGLEYKVNEGDGAFYGPKIDFHVTDSIGREW
QCATIQLDFMMPERFELTYVGPDNKEYRPVMIHRAIYGSLDRFFGILIEHYAGAFPTWLAPVQVVAIPISDKHVDYAREV
ADALAAEGVRVDVDEKQKSTNYKIREAQLNKIPYMLILGDKEKESETITVRLRTGKNIYGMKLAEFSAKLKEEISNRMSE
SIFKFKAD

Sequences:

>Translated_648_residues
MGCKIELPDGSIKEYDHPVTAADVAADLSPALAKRAIGVEVNGELWDLTRELPEECKINLILEKDPRAPEFFRHTFSHIL
AQAVMRIYGSDKVKLGIGPVIENGFYYDFDLGDIRLTEEDLPKIEEEMEKIIKEDLKLERFELPKEEAIKLMKDQNQPYK
VELIEDLESDVATFYRQGEFVDLCRGPHMPSTGKVKYFKLLSVSGAYWRGDERNKMLQRIYGTAFAKKSELEAYLKMMEE
AKKRDHRKIGPQLGLFSFSYEYAPGMPIYHPKGTIVLNELMKFSREVHSKHGYQEVMTPQIMNDVLWHRSGHWDHYRDNM
YFTEKDEQQYAIKPMNCPGHILIYKSRAMSYRDLPLKLFEFGKVHRYERSGVLHGLLRVRGFIQDDAHIFCTREQLKEQL
KDVIRFVDMIYTPFGFEYRADLCTRPEDFMGDVEIWDLATEALKDAIESIGLEYKVNEGDGAFYGPKIDFHVTDSIGREW
QCATIQLDFMMPERFELTYVGPDNKEYRPVMIHRAIYGSLDRFFGILIEHYAGAFPTWLAPVQVVAIPISDKHVDYAREV
ADALAAEGVRVDVDEKQKSTNYKIREAQLNKIPYMLILGDKEKESETITVRLRTGKNIYGMKLAEFSAKLKEEISNRMSE
SIFKFKAD
>Mature_647_residues
GCKIELPDGSIKEYDHPVTAADVAADLSPALAKRAIGVEVNGELWDLTRELPEECKINLILEKDPRAPEFFRHTFSHILA
QAVMRIYGSDKVKLGIGPVIENGFYYDFDLGDIRLTEEDLPKIEEEMEKIIKEDLKLERFELPKEEAIKLMKDQNQPYKV
ELIEDLESDVATFYRQGEFVDLCRGPHMPSTGKVKYFKLLSVSGAYWRGDERNKMLQRIYGTAFAKKSELEAYLKMMEEA
KKRDHRKIGPQLGLFSFSYEYAPGMPIYHPKGTIVLNELMKFSREVHSKHGYQEVMTPQIMNDVLWHRSGHWDHYRDNMY
FTEKDEQQYAIKPMNCPGHILIYKSRAMSYRDLPLKLFEFGKVHRYERSGVLHGLLRVRGFIQDDAHIFCTREQLKEQLK
DVIRFVDMIYTPFGFEYRADLCTRPEDFMGDVEIWDLATEALKDAIESIGLEYKVNEGDGAFYGPKIDFHVTDSIGREWQ
CATIQLDFMMPERFELTYVGPDNKEYRPVMIHRAIYGSLDRFFGILIEHYAGAFPTWLAPVQVVAIPISDKHVDYAREVA
DALAAEGVRVDVDEKQKSTNYKIREAQLNKIPYMLILGDKEKESETITVRLRTGKNIYGMKLAEFSAKLKEEISNRMSES
IFKFKAD

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=644, Percent_Identity=42.5465838509317, Blast_Score=531, Evalue=1e-150,
Organism=Homo sapiens, GI38570111, Length=604, Percent_Identity=43.2119205298013, Blast_Score=519, Evalue=1e-147,
Organism=Homo sapiens, GI20070344, Length=663, Percent_Identity=38.4615384615385, Blast_Score=456, Evalue=1e-128,
Organism=Escherichia coli, GI1788013, Length=636, Percent_Identity=47.0125786163522, Blast_Score=607, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI71984192, Length=614, Percent_Identity=40.5537459283388, Blast_Score=500, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI71984184, Length=614, Percent_Identity=40.5537459283388, Blast_Score=500, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6322112, Length=639, Percent_Identity=41.0015649452269, Blast_Score=507, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6322654, Length=388, Percent_Identity=38.9175257731959, Blast_Score=304, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24583842, Length=632, Percent_Identity=43.0379746835443, Blast_Score=545, Evalue=1e-155,
Organism=Drosophila melanogaster, GI28574141, Length=632, Percent_Identity=43.0379746835443, Blast_Score=545, Evalue=1e-155,
Organism=Drosophila melanogaster, GI24583839, Length=632, Percent_Identity=43.0379746835443, Blast_Score=545, Evalue=1e-155,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_KOSOT (C5CI64)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941770.1
- ProteinModelPortal:   C5CI64
- GeneID:   7968500
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_2089
- ProtClustDB:   PRK00413
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 75171; Mature: 75040

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCKIELPDGSIKEYDHPVTAADVAADLSPALAKRAIGVEVNGELWDLTRELPEECKINL
CCCEEECCCCCHHHCCCCCCHHHHHHHCCHHHHHHHCCEEECCHHHHHHHHCCHHCCEEE
ILEKDPRAPEFFRHTFSHILAQAVMRIYGSDKVKLGIGPVIENGFYYDFDLGDIRLTEED
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCEEEEECCCCEEECHHH
LPKIEEEMEKIIKEDLKLERFELPKEEAIKLMKDQNQPYKVELIEDLESDVATFYRQGEF
HHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCE
VDLCRGPHMPSTGKVKYFKLLSVSGAYWRGDERNKMLQRIYGTAFAKKSELEAYLKMMEE
EEHHCCCCCCCCCCEEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKKRDHRKIGPQLGLFSFSYEYAPGMPIYHPKGTIVLNELMKFSREVHSKHGYQEVMTPQ
HHHHHHHHCCCCCCEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHCCHH
IMNDVLWHRSGHWDHYRDNMYFTEKDEQQYAIKPMNCPGHILIYKSRAMSYRDLPLKLFE
HHHHHHHCCCCCCCCCCCCEEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCCHHHHH
FGKVHRYERSGVLHGLLRVRGFIQDDAHIFCTREQLKEQLKDVIRFVDMIYTPFGFEYRA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEH
DLCTRPEDFMGDVEIWDLATEALKDAIESIGLEYKVNEGDGAFYGPKIDFHVTDSIGREW
HCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCEEEEEEECCCCCCE
QCATIQLDFMMPERFELTYVGPDNKEYRPVMIHRAIYGSLDRFFGILIEHYAGAFPTWLA
EEEEEEEEEECCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHCC
PVQVVAIPISDKHVDYAREVADALAAEGVRVDVDEKQKSTNYKIREAQLNKIPYMLILGD
CEEEEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEHHCCCCEEEEECC
KEKESETITVRLRTGKNIYGMKLAEFSAKLKEEISNRMSESIFKFKAD
CCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GCKIELPDGSIKEYDHPVTAADVAADLSPALAKRAIGVEVNGELWDLTRELPEECKINL
CCEEECCCCCHHHCCCCCCHHHHHHHCCHHHHHHHCCEEECCHHHHHHHHCCHHCCEEE
ILEKDPRAPEFFRHTFSHILAQAVMRIYGSDKVKLGIGPVIENGFYYDFDLGDIRLTEED
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCEEEEECCCCEEECHHH
LPKIEEEMEKIIKEDLKLERFELPKEEAIKLMKDQNQPYKVELIEDLESDVATFYRQGEF
HHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCE
VDLCRGPHMPSTGKVKYFKLLSVSGAYWRGDERNKMLQRIYGTAFAKKSELEAYLKMMEE
EEHHCCCCCCCCCCEEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKKRDHRKIGPQLGLFSFSYEYAPGMPIYHPKGTIVLNELMKFSREVHSKHGYQEVMTPQ
HHHHHHHHCCCCCCEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHCCHH
IMNDVLWHRSGHWDHYRDNMYFTEKDEQQYAIKPMNCPGHILIYKSRAMSYRDLPLKLFE
HHHHHHHCCCCCCCCCCCCEEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCCHHHHH
FGKVHRYERSGVLHGLLRVRGFIQDDAHIFCTREQLKEQLKDVIRFVDMIYTPFGFEYRA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEH
DLCTRPEDFMGDVEIWDLATEALKDAIESIGLEYKVNEGDGAFYGPKIDFHVTDSIGREW
HCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCEEEEEEECCCCCCE
QCATIQLDFMMPERFELTYVGPDNKEYRPVMIHRAIYGSLDRFFGILIEHYAGAFPTWLA
EEEEEEEEEECCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHCC
PVQVVAIPISDKHVDYAREVADALAAEGVRVDVDEKQKSTNYKIREAQLNKIPYMLILGD
CEEEEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEHHCCCCEEEEECC
KEKESETITVRLRTGKNIYGMKLAEFSAKLKEEISNRMSESIFKFKAD
CCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA