Click here to switch to the map view.

The map label for this gene is ndhK [H]

Identifier: 239618414

GI number: 239618414

Start: 2184004

End: 2184543

Strand: Reverse

Name: ndhK [H]

Synonym: Kole_2054

Alternate gene names: 239618414

Gene position: 2184543-2184004 (Counterclockwise)

Preceding gene: 239618415

Following gene: 239618413

Centisome position: 94.89

GC content: 43.7

Gene sequence:

>540_bases
ATGAACGCTGAAAAATCTGCTTGGAATAAAGTGGCAAACATGCTTCGTGCCCGCTCGTTATGGATGCTTTATTACTGTAC
CGGATGCGGTGCAATCGAACTACCGCCAACGATGACCGCAAGATTCGATATGGAACGTTTTGGAATCGGTCCAATGGCAA
CTCCAAGGCAAGCTGATATATTGTTGATAACCGGTTATTTGAGTACGAAAACTCTAAGAAGGGTTATCTATACATACGAA
CAGATGCAGGCACCAAAATATGTAGTAGGATTCGGTTCCTGTACCATCAACGGAGGCATTTACTTCGATTCCTACGCAAC
TATAAACAGACTCGACCTTTATTTGCCTGTTGACTTATATCTGGCTGGTTGTATGCCCAGGCCTGAGGCCATAGTGAACG
GTTTCAAAGAATTGATGAAAAAGATCGATCGTGGCGAAGCCACCGGCTGGAAAACTTATCAGGAAAACTATGAATGGTAC
AAAAAGAATCAATTAAAAGCCCTCGGGGAGGTGTACGTGCACGATGAATTCCATGAATGA

Upstream 100 bases:

>100_bases
TTCAGAGTAGAGCAAGCAGTGAAATATTACTGGAAATGGCCGTTGATTTTGGCCTTCGTTCAAACGATCGTCGTTATCTT
CTGGTAAGGAGGTGCAATGA

Downstream 100 bases:

>100_bases
ACTGATAAAAGAAGTCGAAAACTTATTTGGCACCATAAAATTAAATCAAATCGCTGAAAGAAAATACACTGTTGACACTG
ATCTAAAAGACCTCATTTCT

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K; NDH-1 subunit K; NDH-K [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MNAEKSAWNKVANMLRARSLWMLYYCTGCGAIELPPTMTARFDMERFGIGPMATPRQADILLITGYLSTKTLRRVIYTYE
QMQAPKYVVGFGSCTINGGIYFDSYATINRLDLYLPVDLYLAGCMPRPEAIVNGFKELMKKIDRGEATGWKTYQENYEWY
KKNQLKALGEVYVHDEFHE

Sequences:

>Translated_179_residues
MNAEKSAWNKVANMLRARSLWMLYYCTGCGAIELPPTMTARFDMERFGIGPMATPRQADILLITGYLSTKTLRRVIYTYE
QMQAPKYVVGFGSCTINGGIYFDSYATINRLDLYLPVDLYLAGCMPRPEAIVNGFKELMKKIDRGEATGWKTYQENYEWY
KKNQLKALGEVYVHDEFHE
>Mature_179_residues
MNAEKSAWNKVANMLRARSLWMLYYCTGCGAIELPPTMTARFDMERFGIGPMATPRQADILLITGYLSTKTLRRVIYTYE
QMQAPKYVVGFGSCTINGGIYFDSYATINRLDLYLPVDLYLAGCMPRPEAIVNGFKELMKKIDRGEATGWKTYQENYEWY
KKNQLKALGEVYVHDEFHE

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=136, Percent_Identity=38.2352941176471, Blast_Score=111, Evalue=4e-25,
Organism=Escherichia coli, GI1788624, Length=137, Percent_Identity=35.7664233576642, Blast_Score=97, Evalue=5e-22,
Organism=Escherichia coli, GI1788834, Length=112, Percent_Identity=39.2857142857143, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1789074, Length=110, Percent_Identity=39.0909090909091, Blast_Score=88, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=157, Percent_Identity=35.031847133758, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI18859983, Length=154, Percent_Identity=36.3636363636364, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24642371, Length=154, Percent_Identity=36.3636363636364, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24651058, Length=136, Percent_Identity=38.2352941176471, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 20633; Mature: 20633

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAEKSAWNKVANMLRARSLWMLYYCTGCGAIELPPTMTARFDMERFGIGPMATPRQADI
CCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEECCCCCHHEECHHHHCCCCCCCCCCCCE
LLITGYLSTKTLRRVIYTYEQMQAPKYVVGFGSCTINGGIYFDSYATINRLDLYLPVDLY
EEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCEEECCCCEEEEEEEEECHHHH
LAGCMPRPEAIVNGFKELMKKIDRGEATGWKTYQENYEWYKKNQLKALGEVYVHDEFHE
HCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCC
>Mature Secondary Structure
MNAEKSAWNKVANMLRARSLWMLYYCTGCGAIELPPTMTARFDMERFGIGPMATPRQADI
CCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEECCCCCHHEECHHHHCCCCCCCCCCCCE
LLITGYLSTKTLRRVIYTYEQMQAPKYVVGFGSCTINGGIYFDSYATINRLDLYLPVDLY
EEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCEEECCCCEEEEEEEEECHHHH
LAGCMPRPEAIVNGFKELMKKIDRGEATGWKTYQENYEWYKKNQLKALGEVYVHDEFHE
HCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA