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The map label for this gene is tmk

Identifier: 239618400

GI number: 239618400

Start: 2167239

End: 2167865

Strand: Direct

Name: tmk

Synonym: Kole_2040

Alternate gene names: 239618400

Gene position: 2167239-2167865 (Clockwise)

Preceding gene: 239618399

Following gene: 239618401

Centisome position: 94.14

GC content: 41.79

Gene sequence:

>627_bases
GTGTTTATTTCCTTTGAAGGTATAGATGGTTCCGGCAAATCAACCCAGGTAGCCCTCTTTCGTGAATACCTCGAAAGGAA
TTCGATGGAGTATGTTTTCATAAGAGAGCCCGGAGGTACGCAGGCAGGAGAGGATATAAGAGAGATTCTTTTGCACAACG
AGTATAAACTTTTCCCGGAAACAGAGCTGCTGCTCTTCATGGCTTCACGTGCACAGATTGTTCGCGAGGTTATAATTCCT
GCATTGAAAAAGAAAAAATTGGTCCTCGCAGATCGGTTTCTCGACTCTTCCGTTGCATATCAGGGTTATGGAAGAGGATT
GTCTATTCAGATGGTTACAACATTGAATGAATTCAGTACTGGAGGGGTGACGCCACATTTAACATTGTTTATAGATGTGC
CAGTCGATGTAGCTGTAAAACGCATGCGAAGAGAAATGAAACATGATAAAATAGAAATGGAAAGTTTAGATTTCTTCAAA
AGGGTAAGACAGGGCTATCTAGAACTGGCAAAACGAGATCCGAAAAGGATTCTAGTAATAGATGGAACGATGAGTGTAGA
AAAGATACACGAACAGGTAGTGAAGGAATTTCTTCTTTGCCTCAAAAAGCTGGGGCACGGTCTGTAA

Upstream 100 bases:

>100_bases
ACAAACAATCACAGCTTGTTCTGGTCAAAGGAAGAAAGAATTCAACTTCCGAGGTGGTGGTAGAACCGCCAATTATTTTG
AGAAAAGGGAGGAGCTAGTA

Downstream 100 bases:

>100_bases
CCGGAGGTGATACGATGTATTTCGATAAATTTTCTGAGGGAGCCGCACAGGTATTTGTAGCTGCCCAGGATGAAGCAAGG
AGCCTGGGGCATCCATATGT

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MFISFEGIDGSGKSTQVALFREYLERNSMEYVFIREPGGTQAGEDIREILLHNEYKLFPETELLLFMASRAQIVREVIIP
ALKKKKLVLADRFLDSSVAYQGYGRGLSIQMVTTLNEFSTGGVTPHLTLFIDVPVDVAVKRMRREMKHDKIEMESLDFFK
RVRQGYLELAKRDPKRILVIDGTMSVEKIHEQVVKEFLLCLKKLGHGL

Sequences:

>Translated_208_residues
MFISFEGIDGSGKSTQVALFREYLERNSMEYVFIREPGGTQAGEDIREILLHNEYKLFPETELLLFMASRAQIVREVIIP
ALKKKKLVLADRFLDSSVAYQGYGRGLSIQMVTTLNEFSTGGVTPHLTLFIDVPVDVAVKRMRREMKHDKIEMESLDFFK
RVRQGYLELAKRDPKRILVIDGTMSVEKIHEQVVKEFLLCLKKLGHGL
>Mature_208_residues
MFISFEGIDGSGKSTQVALFREYLERNSMEYVFIREPGGTQAGEDIREILLHNEYKLFPETELLLFMASRAQIVREVIIP
ALKKKKLVLADRFLDSSVAYQGYGRGLSIQMVTTLNEFSTGGVTPHLTLFIDVPVDVAVKRMRREMKHDKIEMESLDFFK
RVRQGYLELAKRDPKRILVIDGTMSVEKIHEQVVKEFLLCLKKLGHGL

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=208, Percent_Identity=39.9038461538462, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_KOSOT (C5CHP2)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941722.1
- GeneID:   7967639
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_2040
- HAMAP:   MF_00165
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 23883; Mature: 23883

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFISFEGIDGSGKSTQVALFREYLERNSMEYVFIREPGGTQAGEDIREILLHNEYKLFPE
CEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCC
TELLLFMASRAQIVREVIIPALKKKKLVLADRFLDSSVAYQGYGRGLSIQMVTTLNEFST
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHCC
GGVTPHLTLFIDVPVDVAVKRMRREMKHDKIEMESLDFFKRVRQGYLELAKRDPKRILVI
CCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DGTMSVEKIHEQVVKEFLLCLKKLGHGL
ECCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFISFEGIDGSGKSTQVALFREYLERNSMEYVFIREPGGTQAGEDIREILLHNEYKLFPE
CEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCC
TELLLFMASRAQIVREVIIPALKKKKLVLADRFLDSSVAYQGYGRGLSIQMVTTLNEFST
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHCC
GGVTPHLTLFIDVPVDVAVKRMRREMKHDKIEMESLDFFKRVRQGYLELAKRDPKRILVI
CCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DGTMSVEKIHEQVVKEFLLCLKKLGHGL
ECCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA