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The map label for this gene is lysS [H]

Identifier: 239618393

GI number: 239618393

Start: 2159459

End: 2160973

Strand: Direct

Name: lysS [H]

Synonym: Kole_2033

Alternate gene names: 239618393

Gene position: 2159459-2160973 (Clockwise)

Preceding gene: 239618392

Following gene: 239618394

Centisome position: 93.8

GC content: 41.98

Gene sequence:

>1515_bases
ATGAACGAACAAATAAGACAACATAAACTCAATGAGATAGAGGAACTTCGTTCCATGGGGATAGAACCGTATCCTCATCA
TTTCGAAAAGACCCATAGTACCAGTGATATCCGCAAAGAATATGATATGCTTCAGCCATCGGAAACCAAAGAAGATACAA
CCATTTCAGTTGCAGGAAGAGTGATGGCTCTCAGGCATCATGGTAAATCATCGTTCTTTGTCTTGAAGGATAGCGATGGA
AGAATACAAGCATATATCAGAAAAGATTCCGTTGGCGACGAAGCATTCAAACTTTTCAAGAAATATGTCAGTATCGGAGA
TTTTGTTGGTGTAAAAGGGTTCCCGTTTAAAACCCACACAGGTGAACTGTCTATTTTTGTTAAGGATTTCAAGCTCCTGT
CCAAAGCTATAAGAACGATGCCTGAAAAATGGCATGGAATAAAGGACAAGGAAATAATTTATAGGCAGAGATACGTGGAA
ATGATTGCCAGCGATGACGCCATTAAAAGATTCAAGATCAGATTCGAGCTCCTAAGACGTGTTAGAGAGTTTCTTAATGC
AAGGGGTTTTATAGAAGTAGAAACGCCGATCCTTCATTCTGTCACCGGTGGAGCTTCCGCAAGACCATTTGTGACCCATA
TCAACGTTTTTGATTCGGATATGTACCTGAGAATCGCTGAGGAGCTATATCTCAAAAGATATCTAGTGGGCGGATTTGAG
AAGATATATGAAATTGGAAAGAATTTCAGGAACGAGGGAATGTCTTATAAGCATCATCCGGAGTTTGTCATGATGGAACT
ATATCAGGCTTATGCCGACTACAACGATATTATGGACCTTACCGAGGATCTTATAAGCACCGTCGTAGAGCAGCTCACAG
GTTCCACGAAGATCGTTTATCAGGGTAAGGAAATAGATTTTGCCAAACCATGGCTGCGTATCAAGATGGCTGATTTCATC
AAAGAAAAGCTCGGAGTTGATATACTTGAAGATTCCGATGAAAGGTTAATTGAGGTTCTTGAGAAACATAACAGTGTTCC
TGAGATCAAAGAAAGAGGCCATCTCATAGAAAAGTTATGGGATCTTGTTGAGGATGATATTGTACAACCTACCTTTGTTG
TTGAACATCCTGTCATCATTTCTCCACTTGCGAAGGTCCACAGAGAAGATCCGAGGGTAACAGAAAGGTTTGAATTAATT
ATAAATGGCATGGAATGTGCGAACGCCTTTAGTGAACTCAATGACCCAATCGAACAGTACAGGAGATTCCTCCATCAGGC
TGGATTAAGAGAAGCCGGTGATGCCGAAGCACAAATGATGGATCGTGATTTCATACGCGCTTTGGAATATGGCATGCCCC
CAACTGGAGGTCTTGGGATTGGTTGGGACAGGATTCTCATGTTCGTTACCGATTCTCCCACCATACGCGATGTGATTCCC
TTCCCGCTGGTAAGGCCGTTGAGTTTTGAAGAAGAGGAGAAAATTGTTACCGATGCAGAGGAAGAGGAAAGCTGA

Upstream 100 bases:

>100_bases
TTTTAGAAAGAAGCGTCGATGATGAAGTTGTTGTAAGAACACCTTCTGGAATTATTAAGTACAAGATAATAACCATCAGC
AGGTAGTGGGGGGTTGGCAA

Downstream 100 bases:

>100_bases
GGAGGGATTTTATGACTCTGTATGAGAGGATACAGGCTGAACTAAAGCAGGCTATGAAAGACAAGGATTCCGTGAAGATG
AATACCTTGAGAGCTGTGGT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MNEQIRQHKLNEIEELRSMGIEPYPHHFEKTHSTSDIRKEYDMLQPSETKEDTTISVAGRVMALRHHGKSSFFVLKDSDG
RIQAYIRKDSVGDEAFKLFKKYVSIGDFVGVKGFPFKTHTGELSIFVKDFKLLSKAIRTMPEKWHGIKDKEIIYRQRYVE
MIASDDAIKRFKIRFELLRRVREFLNARGFIEVETPILHSVTGGASARPFVTHINVFDSDMYLRIAEELYLKRYLVGGFE
KIYEIGKNFRNEGMSYKHHPEFVMMELYQAYADYNDIMDLTEDLISTVVEQLTGSTKIVYQGKEIDFAKPWLRIKMADFI
KEKLGVDILEDSDERLIEVLEKHNSVPEIKERGHLIEKLWDLVEDDIVQPTFVVEHPVIISPLAKVHREDPRVTERFELI
INGMECANAFSELNDPIEQYRRFLHQAGLREAGDAEAQMMDRDFIRALEYGMPPTGGLGIGWDRILMFVTDSPTIRDVIP
FPLVRPLSFEEEEKIVTDAEEEES

Sequences:

>Translated_504_residues
MNEQIRQHKLNEIEELRSMGIEPYPHHFEKTHSTSDIRKEYDMLQPSETKEDTTISVAGRVMALRHHGKSSFFVLKDSDG
RIQAYIRKDSVGDEAFKLFKKYVSIGDFVGVKGFPFKTHTGELSIFVKDFKLLSKAIRTMPEKWHGIKDKEIIYRQRYVE
MIASDDAIKRFKIRFELLRRVREFLNARGFIEVETPILHSVTGGASARPFVTHINVFDSDMYLRIAEELYLKRYLVGGFE
KIYEIGKNFRNEGMSYKHHPEFVMMELYQAYADYNDIMDLTEDLISTVVEQLTGSTKIVYQGKEIDFAKPWLRIKMADFI
KEKLGVDILEDSDERLIEVLEKHNSVPEIKERGHLIEKLWDLVEDDIVQPTFVVEHPVIISPLAKVHREDPRVTERFELI
INGMECANAFSELNDPIEQYRRFLHQAGLREAGDAEAQMMDRDFIRALEYGMPPTGGLGIGWDRILMFVTDSPTIRDVIP
FPLVRPLSFEEEEKIVTDAEEEES
>Mature_504_residues
MNEQIRQHKLNEIEELRSMGIEPYPHHFEKTHSTSDIRKEYDMLQPSETKEDTTISVAGRVMALRHHGKSSFFVLKDSDG
RIQAYIRKDSVGDEAFKLFKKYVSIGDFVGVKGFPFKTHTGELSIFVKDFKLLSKAIRTMPEKWHGIKDKEIIYRQRYVE
MIASDDAIKRFKIRFELLRRVREFLNARGFIEVETPILHSVTGGASARPFVTHINVFDSDMYLRIAEELYLKRYLVGGFE
KIYEIGKNFRNEGMSYKHHPEFVMMELYQAYADYNDIMDLTEDLISTVVEQLTGSTKIVYQGKEIDFAKPWLRIKMADFI
KEKLGVDILEDSDERLIEVLEKHNSVPEIKERGHLIEKLWDLVEDDIVQPTFVVEHPVIISPLAKVHREDPRVTERFELI
INGMECANAFSELNDPIEQYRRFLHQAGLREAGDAEAQMMDRDFIRALEYGMPPTGGLGIGWDRILMFVTDSPTIRDVIP
FPLVRPLSFEEEEKIVTDAEEEES

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=492, Percent_Identity=40.650406504065, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI5031815, Length=492, Percent_Identity=40.650406504065, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI45439306, Length=473, Percent_Identity=22.8329809725159, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI40789249, Length=234, Percent_Identity=26.0683760683761, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790571, Length=499, Percent_Identity=44.689378757515, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1789256, Length=501, Percent_Identity=44.1117764471058, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI87082379, Length=330, Percent_Identity=30.6060606060606, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1788173, Length=240, Percent_Identity=22.9166666666667, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=495, Percent_Identity=41.2121212121212, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17535927, Length=495, Percent_Identity=41.2121212121212, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71994340, Length=495, Percent_Identity=41.2121212121212, Blast_Score=367, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32566633, Length=245, Percent_Identity=27.7551020408163, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17551876, Length=470, Percent_Identity=23.1914893617021, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=512, Percent_Identity=40.8203125, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324256, Length=488, Percent_Identity=29.7131147540984, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6321807, Length=451, Percent_Identity=26.1640798226164, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323011, Length=470, Percent_Identity=22.3404255319149, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24640849, Length=494, Percent_Identity=42.914979757085, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24640851, Length=494, Percent_Identity=42.914979757085, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI17136276, Length=460, Percent_Identity=21.7391304347826, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19921528, Length=446, Percent_Identity=26.457399103139, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24584738, Length=229, Percent_Identity=23.5807860262009, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 58640; Mature: 58640

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQIRQHKLNEIEELRSMGIEPYPHHFEKTHSTSDIRKEYDMLQPSETKEDTTISVAGR
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHH
VMALRHHGKSSFFVLKDSDGRIQAYIRKDSVGDEAFKLFKKYVSIGDFVGVKGFPFKTHT
HHHHHCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GELSIFVKDFKLLSKAIRTMPEKWHGIKDKEIIYRQRYVEMIASDDAIKRFKIRFELLRR
CCEEEHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VREFLNARGFIEVETPILHSVTGGASARPFVTHINVFDSDMYLRIAEELYLKRYLVGGFE
HHHHHCCCCEEEECCCHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHH
KIYEIGKNFRNEGMSYKHHPEFVMMELYQAYADYNDIMDLTEDLISTVVEQLTGSTKIVY
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEE
QGKEIDFAKPWLRIKMADFIKEKLGVDILEDSDERLIEVLEKHNSVPEIKERGHLIEKLW
ECCCCCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
DLVEDDIVQPTFVVEHPVIISPLAKVHREDPRVTERFELIINGMECANAFSELNDPIEQY
HHHHHHCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRFLHQAGLREAGDAEAQMMDRDFIRALEYGMPPTGGLGIGWDRILMFVTDSPTIRDVIP
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCCHHHCCC
FPLVRPLSFEEEEKIVTDAEEEES
CCCCCCCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MNEQIRQHKLNEIEELRSMGIEPYPHHFEKTHSTSDIRKEYDMLQPSETKEDTTISVAGR
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHH
VMALRHHGKSSFFVLKDSDGRIQAYIRKDSVGDEAFKLFKKYVSIGDFVGVKGFPFKTHT
HHHHHCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GELSIFVKDFKLLSKAIRTMPEKWHGIKDKEIIYRQRYVEMIASDDAIKRFKIRFELLRR
CCEEEHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VREFLNARGFIEVETPILHSVTGGASARPFVTHINVFDSDMYLRIAEELYLKRYLVGGFE
HHHHHCCCCEEEECCCHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHH
KIYEIGKNFRNEGMSYKHHPEFVMMELYQAYADYNDIMDLTEDLISTVVEQLTGSTKIVY
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEE
QGKEIDFAKPWLRIKMADFIKEKLGVDILEDSDERLIEVLEKHNSVPEIKERGHLIEKLW
ECCCCCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
DLVEDDIVQPTFVVEHPVIISPLAKVHREDPRVTERFELIINGMECANAFSELNDPIEQY
HHHHHHCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRFLHQAGLREAGDAEAQMMDRDFIRALEYGMPPTGGLGIGWDRILMFVTDSPTIRDVIP
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCCHHHCCC
FPLVRPLSFEEEEKIVTDAEEEES
CCCCCCCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA