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The map label for this gene is greA [H]

Identifier: 239618392

GI number: 239618392

Start: 2158968

End: 2159444

Strand: Direct

Name: greA [H]

Synonym: Kole_2032

Alternate gene names: 239618392

Gene position: 2158968-2159444 (Clockwise)

Preceding gene: 239618391

Following gene: 239618393

Centisome position: 93.78

GC content: 37.32

Gene sequence:

>477_bases
GTGTCTAAAAAGAATGTGATTTACCTTACGAAAGAAGGTTATGAAAACCTGAAAAATGAATTGAATACGCTGAGAAAGAA
GTTGATGTACGAGGTTGCAGAAAGAATAAAAGAAGCAAGAGAATTGGGAGATCTCAGCGAGAATAGTGAATATGATGAAG
CAAAAAACGAGCAAGGAAAGATAGACAGCAGAATCAAAGAACTGGAATACATCCTTGACAATGCCGAAATTATAGAGGAT
GATGGGGATAATAGTGTTGTAAAACTCGGCGCCAAGGTCAGGCTGAGAAATTTAAAAACAAACGAAGAGATTGAGATAAG
AATTGTCAACTCACAGGAAGCCGATATATTCGAGAACAAAATCAGTGCTGACTCACCAATAGGTAAGGCTGTTTTAGAAA
GAAGCGTCGATGATGAAGTTGTTGTAAGAACACCTTCTGGAATTATTAAGTACAAGATAATAACCATCAGCAGGTAG

Upstream 100 bases:

>100_bases
TTTGCATCAGTTTACAAAGAATTCAGAGATTTGGACCATTTCATGGATATAATAAGGGAGCTAAAGGACGAACTGAAGAA
GCGCGACAGGAGGGATTAAG

Downstream 100 bases:

>100_bases
TGGGGGGTTGGCAAATGAACGAACAAATAAGACAACATAAACTCAATGAGATAGAGGAACTTCGTTCCATGGGGATAGAA
CCGTATCCTCATCATTTCGA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSKKNVIYLTKEGYENLKNELNTLRKKLMYEVAERIKEARELGDLSENSEYDEAKNEQGKIDSRIKELEYILDNAEIIED
DGDNSVVKLGAKVRLRNLKTNEEIEIRIVNSQEADIFENKISADSPIGKAVLERSVDDEVVVRTPSGIIKYKIITISR

Sequences:

>Translated_158_residues
MSKKNVIYLTKEGYENLKNELNTLRKKLMYEVAERIKEARELGDLSENSEYDEAKNEQGKIDSRIKELEYILDNAEIIED
DGDNSVVKLGAKVRLRNLKTNEEIEIRIVNSQEADIFENKISADSPIGKAVLERSVDDEVVVRTPSGIIKYKIITISR
>Mature_157_residues
SKKNVIYLTKEGYENLKNELNTLRKKLMYEVAERIKEARELGDLSENSEYDEAKNEQGKIDSRIKELEYILDNAEIIEDD
GDNSVVKLGAKVRLRNLKTNEEIEIRIVNSQEADIFENKISADSPIGKAVLERSVDDEVVVRTPSGIIKYKIITISR

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=154, Percent_Identity=39.6103896103896, Blast_Score=105, Evalue=1e-24,
Organism=Escherichia coli, GI87082261, Length=140, Percent_Identity=37.1428571428571, Blast_Score=77, Evalue=4e-16,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18104; Mature: 17973

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKNVIYLTKEGYENLKNELNTLRKKLMYEVAERIKEARELGDLSENSEYDEAKNEQGK
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCC
IDSRIKELEYILDNAEIIEDDGDNSVVKLGAKVRLRNLKTNEEIEIRIVNSQEADIFENK
HHHHHHHHHHHHCCCEEEECCCCCCEEEECCEEEEECCCCCCEEEEEEECCCCCHHHHHH
ISADSPIGKAVLERSVDDEVVVRTPSGIIKYKIITISR
CCCCCCHHHHHHHCCCCCCEEEECCCCEEEEEEEEECC
>Mature Secondary Structure 
SKKNVIYLTKEGYENLKNELNTLRKKLMYEVAERIKEARELGDLSENSEYDEAKNEQGK
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCC
IDSRIKELEYILDNAEIIEDDGDNSVVKLGAKVRLRNLKTNEEIEIRIVNSQEADIFENK
HHHHHHHHHHHHCCCEEEECCCCCCEEEECCEEEEECCCCCCEEEEEEECCCCCHHHHHH
ISADSPIGKAVLERSVDDEVVVRTPSGIIKYKIITISR
CCCCCCHHHHHHHCCCCCCEEEECCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]