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The map label for this gene is pgk/tpi [H]

Identifier: 239618382

GI number: 239618382

Start: 2152456

End: 2153205

Strand: Direct

Name: pgk/tpi [H]

Synonym: Kole_2022

Alternate gene names: 239618382

Gene position: 2152456-2153205 (Clockwise)

Preceding gene: 239618381

Following gene: 239618383

Centisome position: 93.5

GC content: 42.53

Gene sequence:

>750_bases
ATGGTTGCTGGTAACTGGAAGATGAACAAATCCCCCACGGAAGCTAAACTTTTTGCCGGTTTGCTAGCTTCAAAGATTGG
AAAAGAAACGGTTATGGATATTGTCGTTTTCCCACCGGCAATAGATATACCCTTTGTTGTTGATGTACTAAAAGACACCA
ATATTGGCGTGGGAGTTCAGAATGTTCACCCGAAAGAAAAGGGAGCTTTCACAGGAGAGACCTCGTTGCTGATGGTTAAA
GATCTCAGTGTAGAATATGTTCTTGTTGGTCATTCTGAGCGCAGACATATATTTGGAGAAAGTGATGAGTTCATCAATGA
AAAGGTAAAAGCGGTATTATCCGCGGGTTTGAGGCCTGTTTTATGTGTTGGTGAAACATTGGAAGAACGTGAAAAGGGAC
TTACTAAAAATGTACTTGAACGTCAGATAAGGCTTGGACTTTTTGGTTTAGAGAAAGAGGATGTTGTTAATACAGTAATA
GCATATGAACCAGTATGGGCAATTGGAACAGGTGTTGTGGCCACCCCGGAACAGGCGGAGGAAGCAATGGCTTTCATAAG
ATCGCTGCTCGGGGAGCTTTACGGTATGGAAACTGCTGAAAATGTACGAATACTTTATGGCGGTAGTATAAAACCAGAGA
ATTTTGATGCACTTATACGCCTTAAGAACGTGGATGGAGGGCTCGTGGGCGGCGCGAGTCTCAAAGAAAGTTTTATAGAA
TTAGTTGAAATCGCGAAGCGTTACGTATAG

Upstream 100 bases:

>100_bases
TTTCTACCGGTGGTGGAGCTTCCCTGGAAATGCTGGAAGGTAAGGAACTTCCTGGCATAGCCAGTATTGCAGACGCTGAT
GAAAAAAAAAACGACGGGTG

Downstream 100 bases:

>100_bases
GAAAAATGGATTTTCTTTTGAAGCCGTCCTCAGCGGACGGCTTTTCTGTCTGTAGATGATGAAAATGCTATAATTCTTCA
CAGGGGGTGAAAAGTATGAG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: Phosphoglycerate kinase; Triosephosphate isomerase; TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MVAGNWKMNKSPTEAKLFAGLLASKIGKETVMDIVVFPPAIDIPFVVDVLKDTNIGVGVQNVHPKEKGAFTGETSLLMVK
DLSVEYVLVGHSERRHIFGESDEFINEKVKAVLSAGLRPVLCVGETLEEREKGLTKNVLERQIRLGLFGLEKEDVVNTVI
AYEPVWAIGTGVVATPEQAEEAMAFIRSLLGELYGMETAENVRILYGGSIKPENFDALIRLKNVDGGLVGGASLKESFIE
LVEIAKRYV

Sequences:

>Translated_249_residues
MVAGNWKMNKSPTEAKLFAGLLASKIGKETVMDIVVFPPAIDIPFVVDVLKDTNIGVGVQNVHPKEKGAFTGETSLLMVK
DLSVEYVLVGHSERRHIFGESDEFINEKVKAVLSAGLRPVLCVGETLEEREKGLTKNVLERQIRLGLFGLEKEDVVNTVI
AYEPVWAIGTGVVATPEQAEEAMAFIRSLLGELYGMETAENVRILYGGSIKPENFDALIRLKNVDGGLVGGASLKESFIE
LVEIAKRYV
>Mature_249_residues
MVAGNWKMNKSPTEAKLFAGLLASKIGKETVMDIVVFPPAIDIPFVVDVLKDTNIGVGVQNVHPKEKGAFTGETSLLMVK
DLSVEYVLVGHSERRHIFGESDEFINEKVKAVLSAGLRPVLCVGETLEEREKGLTKNVLERQIRLGLFGLEKEDVVNTVI
AYEPVWAIGTGVVATPEQAEEAMAFIRSLLGELYGMETAENVRILYGGSIKPENFDALIRLKNVDGGLVGGASLKESFIE
LVEIAKRYV

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI226529917, Length=243, Percent_Identity=43.2098765432099, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI4507645, Length=243, Percent_Identity=43.2098765432099, Blast_Score=188, Evalue=3e-48,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=41.2955465587045, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17536593, Length=243, Percent_Identity=46.0905349794239, Blast_Score=197, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6320255, Length=242, Percent_Identity=44.2148760330579, Blast_Score=194, Evalue=9e-51,
Organism=Drosophila melanogaster, GI28572008, Length=242, Percent_Identity=43.801652892562, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI28572006, Length=242, Percent_Identity=43.801652892562, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI28572004, Length=242, Percent_Identity=43.801652892562, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00162 PGK; PF00121 TIM [H]

EC number: =2.7.2.3; =5.3.1.1 [H]

Molecular weight: Translated: 27216; Mature: 27216

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAGNWKMNKSPTEAKLFAGLLASKIGKETVMDIVVFPPAIDIPFVVDVLKDTNIGVGVQ
CCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHEEEEECCCCCCHHHHHHHHCCCCCCCCC
NVHPKEKGAFTGETSLLMVKDLSVEYVLVGHSERRHIFGESDEFINEKVKAVLSAGLRPV
CCCCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEE
LCVGETLEEREKGLTKNVLERQIRLGLFGLEKEDVVNTVIAYEPVWAIGTGVVATPEQAE
EEHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCEECHHHHH
EAMAFIRSLLGELYGMETAENVRILYGGSIKPENFDALIRLKNVDGGLVGGASLKESFIE
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCEECCHHHHHHHHH
LVEIAKRYV
HHHHHHHCC
>Mature Secondary Structure
MVAGNWKMNKSPTEAKLFAGLLASKIGKETVMDIVVFPPAIDIPFVVDVLKDTNIGVGVQ
CCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHEEEEECCCCCCHHHHHHHHCCCCCCCCC
NVHPKEKGAFTGETSLLMVKDLSVEYVLVGHSERRHIFGESDEFINEKVKAVLSAGLRPV
CCCCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEE
LCVGETLEEREKGLTKNVLERQIRLGLFGLEKEDVVNTVIAYEPVWAIGTGVVATPEQAE
EEHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCEECHHHHH
EAMAFIRSLLGELYGMETAENVRILYGGSIKPENFDALIRLKNVDGGLVGGASLKESFIE
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCEECCHHHHHHHHH
LVEIAKRYV
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7859734; 10360571; 9384563; 10591103 [H]