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The map label for this gene is pgk [H]

Identifier: 239618381

GI number: 239618381

Start: 2151239

End: 2152456

Strand: Direct

Name: pgk [H]

Synonym: Kole_2021

Alternate gene names: 239618381

Gene position: 2151239-2152456 (Clockwise)

Preceding gene: 239618380

Following gene: 239618382

Centisome position: 93.45

GC content: 44.66

Gene sequence:

>1218_bases
ATGCTGAAGAAACTTACAGTAAAGGATGTGGATCTGAAGGATAAAAAGGTACTGTTGCGGGTTGACTTCAATGTTCCTAT
AGATAAAGAAACCGGTGAAGTTGCCGATGATACACGCATAAAGGCCGCATTGCCAACAATAAAATATGTTCTTGAAAATG
GTGGAAAAGCCATTCTCGTATCTCACCTTGGACGCCCAAAAGGCAAGAAGGATCCAAAATATAGTTTAAAACCGGTGGCT
AAGAGATTGGAACAACTCCTTGGAAGAACCGTTAGCTTTGTAGATGACTGCATCGGTGAAGGTGCAAAGAAGGTTATAGA
GGCTATGAAAGAAGGGGAAGTTGTCTTACTAGAAAATGTTCGTTTCTACCCCGAAGAAAAGAAAAATGATCCGGAATTCG
CAGAAAAATTGGCTTCTCTTGCCGATATCCATGTGAACGACGCTTTCGGTACCGCTCATAGGGCGCATGCTTCAAACGTT
GGTGTTGCACAATACTTGACAAGTGTTGCTGGTTTTTTAATGGAAAAGGAAATCGCGATGCTTGGCAAAGCCGTTGCCAA
TCCGGAACACCCTTATGTGGTTATACTTGGAGGAGCGAAGGTTTCAGATAAGATAGGTGTTATCACCAACCTGTTGAAGA
AGGCTGACAGAATATTGATCGGTGGTGCCATGATGTTCACATTCCTGAAGGCACTTGGAAAACAGGTTGGCGATTCACTT
GTTGAGGAAGATAAGCTCGAACTGGCCAAGGAGATTATGGAAGAAGCTAGCAAACGGGGTGTAGAGTTTGTTTTACCGAT
TGATACTGTCATAGCGAAGGAAATCGAAGCCGGTGCTGAGAGCAAAGTTGTGAGAATTGATGATGGTATTCCCGAAGGTT
GGAAAGGCCTGGACATAGGCCCTGAGACGATAGAACTCTTTAAGAGTAAACTCAAAGGTGCAAAAACGGTTGTGTGGAAT
GGGCCCATGGGTGTCTTTGAAATTGATGATTTTGCCAAAGGAACGGAAGAGATCGCAAAAGCTCTCGCAGAACTGGAAGA
CGCAACAACCATCGTTGGCGGAGGCGATAGCGCGGCTGCGATAAATAAATTCAATCTTGCAGATAAGGTCAGCCATGTTT
CTACCGGTGGTGGAGCTTCCCTGGAAATGCTGGAAGGTAAGGAACTTCCTGGCATAGCCAGTATTGCAGACGCTGATGAA
AAAAAAAACGACGGGTGA

Upstream 100 bases:

>100_bases
GGTCGAAAAGCTCGAGGCAATGCTTTGAGCTTAAATATAAACCGGGGCCCCTGAATGCTGGGGCCCTTTTTGACAGATTT
ACGTTAAGGGAGGGATAGAA

Downstream 100 bases:

>100_bases
TGGTTGCTGGTAACTGGAAGATGAACAAATCCCCCACGGAAGCTAAACTTTTTGCCGGTTTGCTAGCTTCAAAGATTGGA
AAAGAAACGGTTATGGATAT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: Phosphoglycerate kinase; Triosephosphate isomerase; TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MLKKLTVKDVDLKDKKVLLRVDFNVPIDKETGEVADDTRIKAALPTIKYVLENGGKAILVSHLGRPKGKKDPKYSLKPVA
KRLEQLLGRTVSFVDDCIGEGAKKVIEAMKEGEVVLLENVRFYPEEKKNDPEFAEKLASLADIHVNDAFGTAHRAHASNV
GVAQYLTSVAGFLMEKEIAMLGKAVANPEHPYVVILGGAKVSDKIGVITNLLKKADRILIGGAMMFTFLKALGKQVGDSL
VEEDKLELAKEIMEEASKRGVEFVLPIDTVIAKEIEAGAESKVVRIDDGIPEGWKGLDIGPETIELFKSKLKGAKTVVWN
GPMGVFEIDDFAKGTEEIAKALAELEDATTIVGGGDSAAAINKFNLADKVSHVSTGGGASLEMLEGKELPGIASIADADE
KKNDG

Sequences:

>Translated_405_residues
MLKKLTVKDVDLKDKKVLLRVDFNVPIDKETGEVADDTRIKAALPTIKYVLENGGKAILVSHLGRPKGKKDPKYSLKPVA
KRLEQLLGRTVSFVDDCIGEGAKKVIEAMKEGEVVLLENVRFYPEEKKNDPEFAEKLASLADIHVNDAFGTAHRAHASNV
GVAQYLTSVAGFLMEKEIAMLGKAVANPEHPYVVILGGAKVSDKIGVITNLLKKADRILIGGAMMFTFLKALGKQVGDSL
VEEDKLELAKEIMEEASKRGVEFVLPIDTVIAKEIEAGAESKVVRIDDGIPEGWKGLDIGPETIELFKSKLKGAKTVVWN
GPMGVFEIDDFAKGTEEIAKALAELEDATTIVGGGDSAAAINKFNLADKVSHVSTGGGASLEMLEGKELPGIASIADADE
KKNDG
>Mature_405_residues
MLKKLTVKDVDLKDKKVLLRVDFNVPIDKETGEVADDTRIKAALPTIKYVLENGGKAILVSHLGRPKGKKDPKYSLKPVA
KRLEQLLGRTVSFVDDCIGEGAKKVIEAMKEGEVVLLENVRFYPEEKKNDPEFAEKLASLADIHVNDAFGTAHRAHASNV
GVAQYLTSVAGFLMEKEIAMLGKAVANPEHPYVVILGGAKVSDKIGVITNLLKKADRILIGGAMMFTFLKALGKQVGDSL
VEEDKLELAKEIMEEASKRGVEFVLPIDTVIAKEIEAGAESKVVRIDDGIPEGWKGLDIGPETIELFKSKLKGAKTVVWN
GPMGVFEIDDFAKGTEEIAKALAELEDATTIVGGGDSAAAINKFNLADKVSHVSTGGGASLEMLEGKELPGIASIADADE
KKNDG

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=416, Percent_Identity=46.875, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI31543397, Length=417, Percent_Identity=47.0023980815348, Blast_Score=362, Evalue=1e-100,
Organism=Escherichia coli, GI1789294, Length=393, Percent_Identity=42.7480916030534, Blast_Score=289, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17508823, Length=418, Percent_Identity=48.0861244019139, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6319857, Length=412, Percent_Identity=50, Blast_Score=398, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24581203, Length=418, Percent_Identity=49.0430622009569, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI17136394, Length=416, Percent_Identity=45.6730769230769, Blast_Score=347, Evalue=7e-96,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00162 PGK; PF00121 TIM [H]

EC number: =2.7.2.3; =5.3.1.1 [H]

Molecular weight: Translated: 43528; Mature: 43528

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKLTVKDVDLKDKKVLLRVDFNVPIDKETGEVADDTRIKAALPTIKYVLENGGKAILV
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
SHLGRPKGKKDPKYSLKPVAKRLEQLLGRTVSFVDDCIGEGAKKVIEAMKEGEVVLLENV
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEEECC
RFYPEEKKNDPEFAEKLASLADIHVNDAFGTAHRAHASNVGVAQYLTSVAGFLMEKEIAM
EECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LGKAVANPEHPYVVILGGAKVSDKIGVITNLLKKADRILIGGAMMFTFLKALGKQVGDSL
HHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHH
VEEDKLELAKEIMEEASKRGVEFVLPIDTVIAKEIEAGAESKVVRIDDGIPEGWKGLDIG
HHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCC
PETIELFKSKLKGAKTVVWNGPMGVFEIDDFAKGTEEIAKALAELEDATTIVGGGDSAAA
HHHHHHHHHHCCCCEEEEECCCCCEEECHHHHCCHHHHHHHHHHHHHCCEEECCCCCCHH
INKFNLADKVSHVSTGGGASLEMLEGKELPGIASIADADEKKNDG
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MLKKLTVKDVDLKDKKVLLRVDFNVPIDKETGEVADDTRIKAALPTIKYVLENGGKAILV
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
SHLGRPKGKKDPKYSLKPVAKRLEQLLGRTVSFVDDCIGEGAKKVIEAMKEGEVVLLENV
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEEECC
RFYPEEKKNDPEFAEKLASLADIHVNDAFGTAHRAHASNVGVAQYLTSVAGFLMEKEIAM
EECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LGKAVANPEHPYVVILGGAKVSDKIGVITNLLKKADRILIGGAMMFTFLKALGKQVGDSL
HHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHH
VEEDKLELAKEIMEEASKRGVEFVLPIDTVIAKEIEAGAESKVVRIDDGIPEGWKGLDIG
HHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCC
PETIELFKSKLKGAKTVVWNGPMGVFEIDDFAKGTEEIAKALAELEDATTIVGGGDSAAA
HHHHHHHHHHCCCCEEEEECCCCCEEECHHHHCCHHHHHHHHHHHHHCCEEECCCCCCHH
INKFNLADKVSHVSTGGGASLEMLEGKELPGIASIADADEKKNDG
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7859734; 10360571; 9384563; 10591103 [H]