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The map label for this gene is relA [H]

Identifier: 239618318

GI number: 239618318

Start: 2071525

End: 2073705

Strand: Reverse

Name: relA [H]

Synonym: Kole_1955

Alternate gene names: 239618318

Gene position: 2073705-2071525 (Counterclockwise)

Preceding gene: 239618319

Following gene: 239618317

Centisome position: 90.08

GC content: 41.63

Gene sequence:

>2181_bases
ATGCCCTTACTATATAATGGTGAAGGATCAAAATTAGTTTCCGAAATAGAAAAGATCTTACAAAAAAAGCTAAAACGCAG
AGAACGGGAGAGAATTATTTCCGCATATGAGCTTGCCAGAGAAAACCACGAAGGACAGTACAGAGAATCAGGTGAGCCTT
TTATTTCACATCCCGTTGCAGTAACAAAAATCCTTGCCGAGCTTCAGGCCGATACGGATACATTGGTATCCGGGCTTTTG
CATGACGTGGTTGAGGATTGCAATGTTCCACTCGAAGATGTAGAAAATCGGTTTGGCCCGATAGTTGCCAAAATTGTAGA
TGGTGTAACCAAAATAAATAACCTCAAGTTATCTGAAACACTGGATAAAGCTGAAATAAAATCCAGAATAAAGATAGAAA
CAATTCGCAAAATGCTTCTCGCCCTTTCCAGCGACCCACGTATTATCATTGTTAAACTCGCTGACCGTCTGCATAATATG
AGAACCCTTTATTTCGTTAAAAACGACAAAAAACGAAAGGACAAAGCGCTGGAAACGCTTAATATTTACGCTCCCATCGC
TCATAGAATCGGTATTCACAGAATAAAGTGGGAACTCGAGGATCTTTCCTTCAAGTATCTGTACCCAAATGACTACAATG
AGTTGAAACTGAGAGTGAAGAAGAAACTTCATGAACGTCAGGCAACAATGGACGAATACAGAAATATAGTTGCCAATGAA
TTGAGAAAGCACAGGGTAAACTTTCTGATTGAAGGAAGGGTAAAACACCTTTATAGCATCTGGCAAAAAATGATTCGAAA
GAACAAATCCTTCGATGAGATCTATGACTTAATAGCCCTAAGAATCATAACGGAAGACGAAGTTAGCTGCTATAAAATAC
TTGGAATCGTCCATTCTCTATGGCCACCCGTTCCCGGCAGGTTTAAAGATTACATCGCTGCCCCTAAATCAAATGGGTAT
AAATCACTTCATACCACTTTAATCACCCACAAAGGTGAACCTCTTGAGATCCAGATCCGAAGCGAGAAGATGCACAAAGA
GGCTGAATTCGGGCTTGCAGCTCACTGGATATATAAAGAGGGGATAGACCTGAAAGAACGAAGCTGGTTCCACCAGTTAT
CGAACTGGCACAAGGATTTCATCGAAAGTTTCAAAGATATAGAAAGCGTTTCTAAAGAACTGGAAACAGACGAGGTCTTC
GTGTTTACCCCAAAGGGTGAGGTTATCCATCTTCCAAAAGGTGCGACTCCCATCGATTTTGCGTATGCCATTCACACGGA
AATAGGTCATCATTTGGCAGGTGCAAAGGTTAACGGTAAGATGGTTCCGATAAATTATCAGCTTCAACTGGGTGACAGGG
TTGAAATCATAGTAAACAAGAATAGTGAAGGCCCAAGCCTGGATTGGCTGAACTACGTGCGCTCGAACAGTACAAAAGCC
AAAATAAGACGTTTCTTCAAAACCAAATACAAAAATGAGCTTGTGGAACGGGGTAAAGAAACCTTCCGTAAGATCAGCAA
AAAACTCGCAAAACCAATGGAAGAACTTTTGAACAGCGAGAGCGTCAAAGAATTGATGAAACGTTCTGGTGTCAATACAG
AAAACGAGTTTTTTATGAAGCTCGGAGACGGGACAATAACCATGGCAGAGATGCTGAAATGTCTTGAACCCCAACCGATA
GATATCGAGATCCCCAAGAAATTGTTAAAACCAGTCAAACCAAAGCAGGCCCTCGGTAAAGAGGTATTAATCGCCGGACA
GAGTGGAATAGACGTTCACTTCGCTAAGTGTTGCAATCCTTTACCCGGTGATAAAATCGTTGCAGTTATCAGCAGCCGTG
GAATATCTATACACGTGAGAAATTGCAAGAATCTTATAAACATTGCGGCAGACAAGTTACTGGAAGCCAGCTGGACTTTT
ACCTTAGACGGTCGTTATGATGCCTGGATAAAAGCGGTTTTTGATACAACAGAGAAAAACGTCGTTGGAAGAATCCTGGA
AAAGATTGAAAATAAAAACGCCAAGGTGAAAAAGTATTATGTGAGTTCCGGAGACTGGGGACAAAGTATCCTTGCGGCGA
ATCTGCTGGTCAAAGATATAGGTCATTTAACATCCGTAATGGAAAGCATTAGAGGCATTAGAGGAGTGCAAGCAGTTGCC
AGAACAGGAGGGTTGCCATGA

Upstream 100 bases:

>100_bases
TAAATGCTGTAAAAGCTTTTATGCTCTAAAAGCTCAATAACTCTGAATCAAAACGGATTTTCATGATAAAATAGTTAAAA
GGGCTAAAGAGGTGGAAAAT

Downstream 100 bases:

>100_bases
GAGCAGTCGTACAAAGGGTAAGTGAAGCCGAAGTAAAGGTAGACGGAAAGATTGTTGGTAAAATATCCGATGGTTTGATG
GTACTTCTCGGCGTTCAAAA

Product: (p)ppGpp synthetase I, SpoT/RelA

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 726; Mature: 725

Protein sequence:

>726_residues
MPLLYNGEGSKLVSEIEKILQKKLKRRERERIISAYELARENHEGQYRESGEPFISHPVAVTKILAELQADTDTLVSGLL
HDVVEDCNVPLEDVENRFGPIVAKIVDGVTKINNLKLSETLDKAEIKSRIKIETIRKMLLALSSDPRIIIVKLADRLHNM
RTLYFVKNDKKRKDKALETLNIYAPIAHRIGIHRIKWELEDLSFKYLYPNDYNELKLRVKKKLHERQATMDEYRNIVANE
LRKHRVNFLIEGRVKHLYSIWQKMIRKNKSFDEIYDLIALRIITEDEVSCYKILGIVHSLWPPVPGRFKDYIAAPKSNGY
KSLHTTLITHKGEPLEIQIRSEKMHKEAEFGLAAHWIYKEGIDLKERSWFHQLSNWHKDFIESFKDIESVSKELETDEVF
VFTPKGEVIHLPKGATPIDFAYAIHTEIGHHLAGAKVNGKMVPINYQLQLGDRVEIIVNKNSEGPSLDWLNYVRSNSTKA
KIRRFFKTKYKNELVERGKETFRKISKKLAKPMEELLNSESVKELMKRSGVNTENEFFMKLGDGTITMAEMLKCLEPQPI
DIEIPKKLLKPVKPKQALGKEVLIAGQSGIDVHFAKCCNPLPGDKIVAVISSRGISIHVRNCKNLINIAADKLLEASWTF
TLDGRYDAWIKAVFDTTEKNVVGRILEKIENKNAKVKKYYVSSGDWGQSILAANLLVKDIGHLTSVMESIRGIRGVQAVA
RTGGLP

Sequences:

>Translated_726_residues
MPLLYNGEGSKLVSEIEKILQKKLKRRERERIISAYELARENHEGQYRESGEPFISHPVAVTKILAELQADTDTLVSGLL
HDVVEDCNVPLEDVENRFGPIVAKIVDGVTKINNLKLSETLDKAEIKSRIKIETIRKMLLALSSDPRIIIVKLADRLHNM
RTLYFVKNDKKRKDKALETLNIYAPIAHRIGIHRIKWELEDLSFKYLYPNDYNELKLRVKKKLHERQATMDEYRNIVANE
LRKHRVNFLIEGRVKHLYSIWQKMIRKNKSFDEIYDLIALRIITEDEVSCYKILGIVHSLWPPVPGRFKDYIAAPKSNGY
KSLHTTLITHKGEPLEIQIRSEKMHKEAEFGLAAHWIYKEGIDLKERSWFHQLSNWHKDFIESFKDIESVSKELETDEVF
VFTPKGEVIHLPKGATPIDFAYAIHTEIGHHLAGAKVNGKMVPINYQLQLGDRVEIIVNKNSEGPSLDWLNYVRSNSTKA
KIRRFFKTKYKNELVERGKETFRKISKKLAKPMEELLNSESVKELMKRSGVNTENEFFMKLGDGTITMAEMLKCLEPQPI
DIEIPKKLLKPVKPKQALGKEVLIAGQSGIDVHFAKCCNPLPGDKIVAVISSRGISIHVRNCKNLINIAADKLLEASWTF
TLDGRYDAWIKAVFDTTEKNVVGRILEKIENKNAKVKKYYVSSGDWGQSILAANLLVKDIGHLTSVMESIRGIRGVQAVA
RTGGLP
>Mature_725_residues
PLLYNGEGSKLVSEIEKILQKKLKRRERERIISAYELARENHEGQYRESGEPFISHPVAVTKILAELQADTDTLVSGLLH
DVVEDCNVPLEDVENRFGPIVAKIVDGVTKINNLKLSETLDKAEIKSRIKIETIRKMLLALSSDPRIIIVKLADRLHNMR
TLYFVKNDKKRKDKALETLNIYAPIAHRIGIHRIKWELEDLSFKYLYPNDYNELKLRVKKKLHERQATMDEYRNIVANEL
RKHRVNFLIEGRVKHLYSIWQKMIRKNKSFDEIYDLIALRIITEDEVSCYKILGIVHSLWPPVPGRFKDYIAAPKSNGYK
SLHTTLITHKGEPLEIQIRSEKMHKEAEFGLAAHWIYKEGIDLKERSWFHQLSNWHKDFIESFKDIESVSKELETDEVFV
FTPKGEVIHLPKGATPIDFAYAIHTEIGHHLAGAKVNGKMVPINYQLQLGDRVEIIVNKNSEGPSLDWLNYVRSNSTKAK
IRRFFKTKYKNELVERGKETFRKISKKLAKPMEELLNSESVKELMKRSGVNTENEFFMKLGDGTITMAEMLKCLEPQPID
IEIPKKLLKPVKPKQALGKEVLIAGQSGIDVHFAKCCNPLPGDKIVAVISSRGISIHVRNCKNLINIAADKLLEASWTFT
LDGRYDAWIKAVFDTTEKNVVGRILEKIENKNAKVKKYYVSSGDWGQSILAANLLVKDIGHLTSVMESIRGIRGVQAVAR
TGGLP

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=140, Percent_Identity=36.4285714285714, Blast_Score=74, Evalue=6e-13,
Organism=Escherichia coli, GI1790082, Length=723, Percent_Identity=37.7593360995851, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1789147, Length=626, Percent_Identity=35.9424920127796, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24650996, Length=137, Percent_Identity=35.036496350365, Blast_Score=68, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 83162; Mature: 83031

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLYNGEGSKLVSEIEKILQKKLKRRERERIISAYELARENHEGQYRESGEPFISHPVA
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCHHH
VTKILAELQADTDTLVSGLLHDVVEDCNVPLEDVENRFGPIVAKIVDGVTKINNLKLSET
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LDKAEIKSRIKIETIRKMLLALSSDPRIIIVKLADRLHNMRTLYFVKNDKKRKDKALETL
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCEEEEEEECCCHHHHHHHHHH
NIYAPIAHRIGIHRIKWELEDLSFKYLYPNDYNELKLRVKKKLHERQATMDEYRNIVANE
HHHHHHHHHCCHHHEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRKHRVNFLIEGRVKHLYSIWQKMIRKNKSFDEIYDLIALRIITEDEVSCYKILGIVHSL
HHHHHHCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
WPPVPGRFKDYIAAPKSNGYKSLHTTLITHKGEPLEIQIRSEKMHKEAEFGLAAHWIYKE
CCCCCCCHHHHHCCCCCCCCHHHHHHHEECCCCCEEEEECHHHHHHHHHCCHHHHHHHHC
GIDLKERSWFHQLSNWHKDFIESFKDIESVSKELETDEVFVFTPKGEVIHLPKGATPIDF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCHH
AYAIHTEIGHHLAGAKVNGKMVPINYQLQLGDRVEIIVNKNSEGPSLDWLNYVRSNSTKA
HHHHHHHHHHHHCCCEECCEEEEEEEEEEECCEEEEEEECCCCCCCHHHHHHHHCCCHHH
KIRRFFKTKYKNELVERGKETFRKISKKLAKPMEELLNSESVKELMKRSGVNTENEFFMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEE
LGDGTITMAEMLKCLEPQPIDIEIPKKLLKPVKPKQALGKEVLIAGQSGIDVHFAKCCNP
ECCCCEEHHHHHHHCCCCCCCEECCHHHCCCCCCHHHCCCEEEEECCCCCCEEHHHHCCC
LPGDKIVAVISSRGISIHVRNCKNLINIAADKLLEASWTFTLDGRYDAWIKAVFDTTEKN
CCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHH
VVGRILEKIENKNAKVKKYYVSSGDWGQSILAANLLVKDIGHLTSVMESIRGIRGVQAVA
HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTGGLP
HCCCCC
>Mature Secondary Structure 
PLLYNGEGSKLVSEIEKILQKKLKRRERERIISAYELARENHEGQYRESGEPFISHPVA
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCHHH
VTKILAELQADTDTLVSGLLHDVVEDCNVPLEDVENRFGPIVAKIVDGVTKINNLKLSET
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LDKAEIKSRIKIETIRKMLLALSSDPRIIIVKLADRLHNMRTLYFVKNDKKRKDKALETL
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCEEEEEEECCCHHHHHHHHHH
NIYAPIAHRIGIHRIKWELEDLSFKYLYPNDYNELKLRVKKKLHERQATMDEYRNIVANE
HHHHHHHHHCCHHHEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRKHRVNFLIEGRVKHLYSIWQKMIRKNKSFDEIYDLIALRIITEDEVSCYKILGIVHSL
HHHHHHCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
WPPVPGRFKDYIAAPKSNGYKSLHTTLITHKGEPLEIQIRSEKMHKEAEFGLAAHWIYKE
CCCCCCCHHHHHCCCCCCCCHHHHHHHEECCCCCEEEEECHHHHHHHHHCCHHHHHHHHC
GIDLKERSWFHQLSNWHKDFIESFKDIESVSKELETDEVFVFTPKGEVIHLPKGATPIDF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCHH
AYAIHTEIGHHLAGAKVNGKMVPINYQLQLGDRVEIIVNKNSEGPSLDWLNYVRSNSTKA
HHHHHHHHHHHHCCCEECCEEEEEEEEEEECCEEEEEEECCCCCCCHHHHHHHHCCCHHH
KIRRFFKTKYKNELVERGKETFRKISKKLAKPMEELLNSESVKELMKRSGVNTENEFFMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEE
LGDGTITMAEMLKCLEPQPIDIEIPKKLLKPVKPKQALGKEVLIAGQSGIDVHFAKCCNP
ECCCCEEHHHHHHHCCCCCCCEECCHHHCCCCCCHHHCCCEEEEECCCCCCEEHHHHCCC
LPGDKIVAVISSRGISIHVRNCKNLINIAADKLLEASWTFTLDGRYDAWIKAVFDTTEKN
CCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHH
VVGRILEKIENKNAKVKKYYVSSGDWGQSILAANLLVKDIGHLTSVMESIRGIRGVQAVA
HHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTGGLP
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8825780; 8631867; 12000953 [H]