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The map label for this gene is mraW

Identifier: 239618274

GI number: 239618274

Start: 2032002

End: 2032898

Strand: Reverse

Name: mraW

Synonym: Kole_1911

Alternate gene names: 239618274

Gene position: 2032898-2032002 (Counterclockwise)

Preceding gene: 239618275

Following gene: 239618273

Centisome position: 88.31

GC content: 45.04

Gene sequence:

>897_bases
TTGCAAAGACAGTACAATGAGTCTCACCGGAGCGTGATGGTCCAGGAAACGCTCCATTATTTGCGTCCTACCTATAATGG
AGTTTATGTGGACGGCACGCTCGGCGAAGGTGGGCATACAAAAGCAATAATTGAAGCAACGTCAGGCAAATGTAAAGTAA
TAGGTCTTGATATCGATGAAGAGGTTCTAGCAATAGCGGAGCAGAACCTGAAAGAGTTTAAGGAGAACGTAGAGTTGTTC
AACGTGTCTTATGTTGATTTCGACTTGGTCCTTGAAAGTTTAGCGGTCGATAAAGTGGATGGATTTCTCCTGGACATCGG
GGTTTCGACTTATCAGTTAAAGGCTAAAGGTCGCGGTTTTTCGTACGAGATTGATGAACCTCTGGATATGAGAATGAGTC
TATCGGGTTCAGTCACAGCTGCAGATGTCGTTAACAGCTATCCCGAGAAAGAACTCGCAAGGATCATTTTTGAGTATGGT
GAAGAAAAACGTTATGCCAGGAGAATTGCGAGGAAGATAGTTGAGCGGAGACCGATTCAAACAACCATTCAGCTTGTGGA
AGCCATTAAAGCAGCACTACCTCCACAGGAGCGATTCAGGAGAAAAAGGCATTATGCCACCAGAACCTTTCAAGCTATCA
GAATTGAGGTCAACGGCGAACTCAAAGGTTTGAGAACAGCTCTTGAGAAGTTTCCAAACTACCTGAAACCTGGTGGAAGG
ATCGTTATTATTTCGTTTCATTCGCTCGAAGACAGGACGGTCAAACATTTTTTCCGGGAACAAGATGGTGTCACCTTAAA
AATTTTGACCAGGAAACCTGTTGTACCGTCTGTCGAAGAAGTGAGTGAAAATCCTCGCGCAAGGAGTGCGAAACTTAGGG
CAGCGGAGCGGATCTGA

Upstream 100 bases:

>100_bases
CGTCAATTGATGAAGACAAAGAATAGCAAGAATTTTTTCTCAAAATTTGACATTGAAGACATAAAAATGATATCATTTTG
ATTGAGCGGAGAGGATCACA

Downstream 100 bases:

>100_bases
AGGGAGGAACATCTCGTGCAAAGCATTACAGCGAAGCGGAGAGCGGTAAGGGCCAGTGTTCATGAAAGAACGCTGGAAAT
CAGTGGTGTCAGGAGTCTTC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MQRQYNESHRSVMVQETLHYLRPTYNGVYVDGTLGEGGHTKAIIEATSGKCKVIGLDIDEEVLAIAEQNLKEFKENVELF
NVSYVDFDLVLESLAVDKVDGFLLDIGVSTYQLKAKGRGFSYEIDEPLDMRMSLSGSVTAADVVNSYPEKELARIIFEYG
EEKRYARRIARKIVERRPIQTTIQLVEAIKAALPPQERFRRKRHYATRTFQAIRIEVNGELKGLRTALEKFPNYLKPGGR
IVIISFHSLEDRTVKHFFREQDGVTLKILTRKPVVPSVEEVSENPRARSAKLRAAERI

Sequences:

>Translated_298_residues
MQRQYNESHRSVMVQETLHYLRPTYNGVYVDGTLGEGGHTKAIIEATSGKCKVIGLDIDEEVLAIAEQNLKEFKENVELF
NVSYVDFDLVLESLAVDKVDGFLLDIGVSTYQLKAKGRGFSYEIDEPLDMRMSLSGSVTAADVVNSYPEKELARIIFEYG
EEKRYARRIARKIVERRPIQTTIQLVEAIKAALPPQERFRRKRHYATRTFQAIRIEVNGELKGLRTALEKFPNYLKPGGR
IVIISFHSLEDRTVKHFFREQDGVTLKILTRKPVVPSVEEVSENPRARSAKLRAAERI
>Mature_298_residues
MQRQYNESHRSVMVQETLHYLRPTYNGVYVDGTLGEGGHTKAIIEATSGKCKVIGLDIDEEVLAIAEQNLKEFKENVELF
NVSYVDFDLVLESLAVDKVDGFLLDIGVSTYQLKAKGRGFSYEIDEPLDMRMSLSGSVTAADVVNSYPEKELARIIFEYG
EEKRYARRIARKIVERRPIQTTIQLVEAIKAALPPQERFRRKRHYATRTFQAIRIEVNGELKGLRTALEKFPNYLKPGGR
IVIISFHSLEDRTVKHFFREQDGVTLKILTRKPVVPSVEEVSENPRARSAKLRAAERI

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=338, Percent_Identity=37.2781065088757, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=38.0952380952381, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1786270, Length=319, Percent_Identity=39.8119122257053, Blast_Score=209, Evalue=1e-55,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=37.4622356495468, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=37.4622356495468, Blast_Score=167, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_KOSOT (C5CGS3)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941596.1
- ProteinModelPortal:   C5CGS3
- GeneID:   7968402
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1911
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34046; Mature: 34046

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: BINDING 57-57 BINDING 91-91 BINDING 105-105 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRQYNESHRSVMVQETLHYLRPTYNGVYVDGTLGEGGHTKAIIEATSGKCKVIGLDIDE
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEECCCCEEEEEECCCH
EVLAIAEQNLKEFKENVELFNVSYVDFDLVLESLAVDKVDGFLLDIGVSTYQLKAKGRGF
HHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCC
SYEIDEPLDMRMSLSGSVTAADVVNSYPEKELARIIFEYGEEKRYARRIARKIVERRPIQ
EEECCCCCCCEEECCCCCHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHH
TTIQLVEAIKAALPPQERFRRKRHYATRTFQAIRIEVNGELKGLRTALEKFPNYLKPGGR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCE
IVIISFHSLEDRTVKHFFREQDGVTLKILTRKPVVPSVEEVSENPRARSAKLRAAERI
EEEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQRQYNESHRSVMVQETLHYLRPTYNGVYVDGTLGEGGHTKAIIEATSGKCKVIGLDIDE
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEECCCCEEEEEECCCH
EVLAIAEQNLKEFKENVELFNVSYVDFDLVLESLAVDKVDGFLLDIGVSTYQLKAKGRGF
HHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCC
SYEIDEPLDMRMSLSGSVTAADVVNSYPEKELARIIFEYGEEKRYARRIARKIVERRPIQ
EEECCCCCCCEEECCCCCHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHH
TTIQLVEAIKAALPPQERFRRKRHYATRTFQAIRIEVNGELKGLRTALEKFPNYLKPGGR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCE
IVIISFHSLEDRTVKHFFREQDGVTLKILTRKPVVPSVEEVSENPRARSAKLRAAERI
EEEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA