Click here to switch to the map view.

The map label for this gene is prfB

Identifier: 239618275

GI number: 239618275

Start: 2032973

End: 2034085

Strand: Reverse

Name: prfB

Synonym: Kole_1912

Alternate gene names: 239618275

Gene position: 2034085-2032973 (Counterclockwise)

Preceding gene: 239618276

Following gene: 239618274

Centisome position: 88.36

GC content: 41.87

Gene sequence:

>1113_bases
ATGTTAAGTTATGAAACAAACGCCAGAATCCAGGAACTGAGAGACAAATTTGAAAATATAAAAACAACCGTTGACGTGGA
TAAGATAAAAGAAAAGCTTTCCCGGATAGAAAAAGAGCTTTCCGACCCGTCCGTTTGGTCGGACCAGCGGCGTGCAGGAA
AACTCGGACAACAGGCTCAAGCTTTAAGAAGTCAGCTCGATTTACTCAATAAAGTCCAGGAACTGTTTGAAAACATAGAT
ATCGCCGTTGAACTCTCAGAAGAAGACGAAAGCTATCTTGAAAACCTCAACGAGCTTCTGAAAGAAGCTGAAGATATGGT
AAAAGAGTTCGAACTAAATATCTTATTGAGTAGTCCTTATGATGCTTACAATGCTTATTTGTCAGTTCATCCGGGAGCTG
GTGGAACAGAATCTCAGGATTGGGCTTCCATGCTATTAAGAATGTACATACGCTGGGCTGAGCGTAATAATTACAAAGTA
ACTACTATAGAAGAACAGCCAGGCGAAGAAGCTGGAATAAAGAGTGCCACTATAAATATAGCCGGCCCTTATGCTTATGG
AAAATTGAAATATGAAGCGGGAGTTCACAGGCTCGTAAGGATATCACCCTTTGACGCTAACCACAGAAGACACACTTCAT
TCGCTTCTGTGAGTGTCTTCCCTGAAATGGACGACGATGTGGAGATAGATATCAGACCTGAGGATCTCAAAATCGATACC
TATCGTGCTGGTGGAGCCGGCGGGCAACATGTCAATCGAACGGAATCCGCGGTGAGAATTACCCATATTCCAACGGGAAT
AGTTGTTACCTGTCAAAACGAACGCTCACAGCATCAGAACAAAGCAACCGCAATGAAAATTCTTAAAGCAAAATTGTTTG
AGCTGGAATTAGAAAAGAAGCGTCAAGAAAAAATGAAATTGATGGGTGAGCAAAAAGACATCGCGTGGGGCAATCAGATA
CGGTCCTATGTTTTCCAGCCTTATACCATGGTAAAAGACCACAGAACCAATTACGAAACAGGAGACGTCCAGGCTGTCAT
GGATGGATACATAGATGATTTCATTGAAAAAGAACTTCTGTTTTTTGCGTCAATTGATGAAGACAAAGAATAG

Upstream 100 bases:

>100_bases
GCCCTCAAAAAAGATGGCGGTAAACGTTCTAAAGAGAAAAAGCACGGTACTCATAGCAGAAGATTCAAAGTAAAACGTTG
AAGGATGGTGGGAACCAACA

Downstream 100 bases:

>100_bases
CAAGAATTTTTTCTCAAAATTTGACATTGAAGACATAAAAATGATATCATTTTGATTGAGCGGAGAGGATCACATTGCAA
AGACAGTACAATGAGTCTCA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MLSYETNARIQELRDKFENIKTTVDVDKIKEKLSRIEKELSDPSVWSDQRRAGKLGQQAQALRSQLDLLNKVQELFENID
IAVELSEEDESYLENLNELLKEAEDMVKEFELNILLSSPYDAYNAYLSVHPGAGGTESQDWASMLLRMYIRWAERNNYKV
TTIEEQPGEEAGIKSATINIAGPYAYGKLKYEAGVHRLVRISPFDANHRRHTSFASVSVFPEMDDDVEIDIRPEDLKIDT
YRAGGAGGQHVNRTESAVRITHIPTGIVVTCQNERSQHQNKATAMKILKAKLFELELEKKRQEKMKLMGEQKDIAWGNQI
RSYVFQPYTMVKDHRTNYETGDVQAVMDGYIDDFIEKELLFFASIDEDKE

Sequences:

>Translated_370_residues
MLSYETNARIQELRDKFENIKTTVDVDKIKEKLSRIEKELSDPSVWSDQRRAGKLGQQAQALRSQLDLLNKVQELFENID
IAVELSEEDESYLENLNELLKEAEDMVKEFELNILLSSPYDAYNAYLSVHPGAGGTESQDWASMLLRMYIRWAERNNYKV
TTIEEQPGEEAGIKSATINIAGPYAYGKLKYEAGVHRLVRISPFDANHRRHTSFASVSVFPEMDDDVEIDIRPEDLKIDT
YRAGGAGGQHVNRTESAVRITHIPTGIVVTCQNERSQHQNKATAMKILKAKLFELELEKKRQEKMKLMGEQKDIAWGNQI
RSYVFQPYTMVKDHRTNYETGDVQAVMDGYIDDFIEKELLFFASIDEDKE
>Mature_370_residues
MLSYETNARIQELRDKFENIKTTVDVDKIKEKLSRIEKELSDPSVWSDQRRAGKLGQQAQALRSQLDLLNKVQELFENID
IAVELSEEDESYLENLNELLKEAEDMVKEFELNILLSSPYDAYNAYLSVHPGAGGTESQDWASMLLRMYIRWAERNNYKV
TTIEEQPGEEAGIKSATINIAGPYAYGKLKYEAGVHRLVRISPFDANHRRHTSFASVSVFPEMDDDVEIDIRPEDLKIDT
YRAGGAGGQHVNRTESAVRITHIPTGIVVTCQNERSQHQNKATAMKILKAKLFELELEKKRQEKMKLMGEQKDIAWGNQI
RSYVFQPYTMVKDHRTNYETGDVQAVMDGYIDDFIEKELLFFASIDEDKE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=263, Percent_Identity=39.1634980988593, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI34577120, Length=297, Percent_Identity=35.6902356902357, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI166795305, Length=206, Percent_Identity=36.8932038834951, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI2367172, Length=357, Percent_Identity=50.9803921568627, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1787462, Length=339, Percent_Identity=36.5781710914454, Blast_Score=205, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17542784, Length=265, Percent_Identity=36.9811320754717, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321295, Length=298, Percent_Identity=36.5771812080537, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI19921226, Length=253, Percent_Identity=36.7588932806324, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_KOSOT (C5CGS4)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941597.1
- GeneID:   7968403
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1912
- ProtClustDB:   PRK00578
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 42513; Mature: 42513

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSYETNARIQELRDKFENIKTTVDVDKIKEKLSRIEKELSDPSVWSDQRRAGKLGQQAQ
CCCCCCCHHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
ALRSQLDLLNKVQELFENIDIAVELSEEDESYLENLNELLKEAEDMVKEFELNILLSSPY
HHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
DAYNAYLSVHPGAGGTESQDWASMLLRMYIRWAERNNYKVTTIEEQPGEEAGIKSATINI
CHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEE
AGPYAYGKLKYEAGVHRLVRISPFDANHRRHTSFASVSVFPEMDDDVEIDIRPEDLKIDT
ECCCEECEEEHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEEE
YRAGGAGGQHVNRTESAVRITHIPTGIVVTCQNERSQHQNKATAMKILKAKLFELELEKK
EECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQEKMKLMGEQKDIAWGNQIRSYVFQPYTMVKDHRTNYETGDVQAVMDGYIDDFIEKELL
HHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FFASIDEDKE
EEEECCCCCC
>Mature Secondary Structure
MLSYETNARIQELRDKFENIKTTVDVDKIKEKLSRIEKELSDPSVWSDQRRAGKLGQQAQ
CCCCCCCHHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
ALRSQLDLLNKVQELFENIDIAVELSEEDESYLENLNELLKEAEDMVKEFELNILLSSPY
HHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
DAYNAYLSVHPGAGGTESQDWASMLLRMYIRWAERNNYKVTTIEEQPGEEAGIKSATINI
CHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEE
AGPYAYGKLKYEAGVHRLVRISPFDANHRRHTSFASVSVFPEMDDDVEIDIRPEDLKIDT
ECCCEECEEEHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEEE
YRAGGAGGQHVNRTESAVRITHIPTGIVVTCQNERSQHQNKATAMKILKAKLFELELEKK
EECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQEKMKLMGEQKDIAWGNQIRSYVFQPYTMVKDHRTNYETGDVQAVMDGYIDDFIEKELL
HHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FFASIDEDKE
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA