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The map label for this gene is nfi

Identifier: 239618232

GI number: 239618232

Start: 2005152

End: 2005832

Strand: Direct

Name: nfi

Synonym: Kole_1869

Alternate gene names: 239618232

Gene position: 2005152-2005832 (Clockwise)

Preceding gene: 239618222

Following gene: 239618289

Centisome position: 87.1

GC content: 39.21

Gene sequence:

>681_bases
ATGAAAATTCGGTGCTTACACGATTGGAATGTTTCTCTGGAAGAAGCCGCTGATATCCAGAGAACCCTGAAAGGAATGTT
ATCCTTTGAATTTCCTGCGAAAAAGGTTTCAATCGTTGCAGGTGTGGATGTTTCTTTTCCTCAGAAGAATCTAGGGCTCT
GTGTGATAGTAGTGATGGATGATACTTTGAAAGTGATTGAAAGTGTCTATCACACCCAGGAAGTTCATATTCCTTATGTG
TCGGGGTTCCTTTCCTTTCGAGAAGGCCCAATATTCATTGAAACAGTGAAAAAATTGAAAATAGTTCCTGACCTTTTCTT
TTTTGATGGTCAGGGAATAGCGCATCCTCGAGGCCTTGGCATTGCTGCTCATATGGGATTGTTATTGGAAAAGCCATCTT
TAGGTGTTGCAAAAAGTCATTTGTTTGGTAGCTACAACGAACCAGGTAGAAATAAAGGAGATTTCTCTTATATGTATAAT
AAAACCGGGGAGATAATTGGAACAGTTTTAAGAACCAAAAAAAATACAAAACCTGTTTTTGTTTCACCGGGCCATATGAT
GGATGTTGATACCGCTATGTCATTAACCCTAAAATATACAGGAAAATATAGGTTACCAGAACCAACAAGGCAGGCTCACA
TACTTACCCAGCGTTTGAGAAAAAATCACCTCTTGCGATAG

Upstream 100 bases:

>100_bases
GAAAATCTCATTTAGACACCTCCAAACGTCCATCTTCTACCTTTATATTTTAACCTAACAGAGGGTATAATTCCAACGAA
CCCTTATAAAGGAGGTTAGC

Downstream 100 bases:

>100_bases
AAAACGATGGAAGCGGCCCCTTTTATTCCGGCATCTTCCACCAGATCACTTAGATCTACTTTGGCCGTTCCCTTGAATGA
AGTCATTACGTATCTATCAA

Product: Deoxyribonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKIRCLHDWNVSLEEAADIQRTLKGMLSFEFPAKKVSIVAGVDVSFPQKNLGLCVIVVMDDTLKVIESVYHTQEVHIPYV
SGFLSFREGPIFIETVKKLKIVPDLFFFDGQGIAHPRGLGIAAHMGLLLEKPSLGVAKSHLFGSYNEPGRNKGDFSYMYN
KTGEIIGTVLRTKKNTKPVFVSPGHMMDVDTAMSLTLKYTGKYRLPEPTRQAHILTQRLRKNHLLR

Sequences:

>Translated_226_residues
MKIRCLHDWNVSLEEAADIQRTLKGMLSFEFPAKKVSIVAGVDVSFPQKNLGLCVIVVMDDTLKVIESVYHTQEVHIPYV
SGFLSFREGPIFIETVKKLKIVPDLFFFDGQGIAHPRGLGIAAHMGLLLEKPSLGVAKSHLFGSYNEPGRNKGDFSYMYN
KTGEIIGTVLRTKKNTKPVFVSPGHMMDVDTAMSLTLKYTGKYRLPEPTRQAHILTQRLRKNHLLR
>Mature_226_residues
MKIRCLHDWNVSLEEAADIQRTLKGMLSFEFPAKKVSIVAGVDVSFPQKNLGLCVIVVMDDTLKVIESVYHTQEVHIPYV
SGFLSFREGPIFIETVKKLKIVPDLFFFDGQGIAHPRGLGIAAHMGLLLEKPSLGVAKSHLFGSYNEPGRNKGDFSYMYN
KTGEIIGTVLRTKKNTKPVFVSPGHMMDVDTAMSLTLKYTGKYRLPEPTRQAHILTQRLRKNHLLR

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=204, Percent_Identity=33.8235294117647, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI257467548, Length=131, Percent_Identity=32.824427480916, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI257467550, Length=131, Percent_Identity=32.824427480916, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI87082357, Length=177, Percent_Identity=37.8531073446328, Blast_Score=131, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71981509, Length=217, Percent_Identity=27.6497695852535, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_KOSOT (C5CGG9)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941554.1
- ProteinModelPortal:   C5CGG9
- GeneID:   7967791
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1869
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 25428; Mature: 25428

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRCLHDWNVSLEEAADIQRTLKGMLSFEFPAKKVSIVAGVDVSFPQKNLGLCVIVVMD
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEC
DTLKVIESVYHTQEVHIPYVSGFLSFREGPIFIETVKKLKIVPDLFFFDGQGIAHPRGLG
CHHHHHHHHHHHCEECCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCC
IAAHMGLLLEKPSLGVAKSHLFGSYNEPGRNKGDFSYMYNKTGEIIGTVLRTKKNTKPVF
HHHHHHHEEECCCCCCHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCEE
VSPGHMMDVDTAMSLTLKYTGKYRLPEPTRQAHILTQRLRKNHLLR
ECCCCEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIRCLHDWNVSLEEAADIQRTLKGMLSFEFPAKKVSIVAGVDVSFPQKNLGLCVIVVMD
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEC
DTLKVIESVYHTQEVHIPYVSGFLSFREGPIFIETVKKLKIVPDLFFFDGQGIAHPRGLG
CHHHHHHHHHHHCEECCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCC
IAAHMGLLLEKPSLGVAKSHLFGSYNEPGRNKGDFSYMYNKTGEIIGTVLRTKKNTKPVF
HHHHHHHEEECCCCCCHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCEE
VSPGHMMDVDTAMSLTLKYTGKYRLPEPTRQAHILTQRLRKNHLLR
ECCCCEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA