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The map label for this gene is glnA [H]

Identifier: 239618112

GI number: 239618112

Start: 1874878

End: 1876275

Strand: Direct

Name: glnA [H]

Synonym: Kole_1744

Alternate gene names: 239618112

Gene position: 1874878-1876275 (Clockwise)

Preceding gene: 239618094

Following gene: 239618116

Centisome position: 81.44

GC content: 43.63

Gene sequence:

>1398_bases
ATGAAAGACTCGTTGAGGAAATATGTTGGAAACATAGATTTTATTGATTTGAAAGTTATCGATATTATTGGAAGGGTGCG
GCACGTAATTCTTCCCGGAGATCGGTTGACCGACGAGCTTTTTGAAGAAGGCGTTGGTTTTGATGCCTCCAACTTTGGTT
ATTCCTCTATAGAAAACAGCGATATGATAATGCTGCCGGATCCAGATACTGCCTTTGAAGATCCCTTCTTTGAGCGCAAA
ACCCTTTCTTTTTTCTGCGATGTTCTCAGAACAGAAGATCGCAAGCCCTTTGAACACTATCCCAGAAACGTTCTAAGAGC
GACGCTTGAGAAACTGAAATCCATTGGAGCTGCAGATGCGATGCTAGGCCCGGAACTGGAATTTCACATCTTTGAAGGAA
TGAGTTACAGAGTTTCGGAAAATCAGGCTTATTTTAAGGTCGATGTTGCCGAAGGTTTCTGGAATAGCGGAGAGTTTTCA
AATGCGAATGTTGTTGAGCGGAAGATGGGATATCATAGGTGCCCCCCTGCGGATGCCATGGCGAATTTCAGAAACGAAGC
CGTTATGATTATGAATTCGATTGGAATTCCAGTTAAATACCATCATCATGAAGTGAGTTCTGCGCAACACGAAATAGAGT
TTCGTTTTCAAAATGCCTTGAATAGTGCAGATTCAATAATCCTTGCCAGGTACATTCTTCAAAACCTGGCTTCCCGTTAC
AACCTAAAGGTTACCTTTATGCCCAAACCGCTTTTCGAGGAAGCCGGAAACGGTATGCACATTCACCAGTACCTTGTAGA
TGGAACAGGTAAAAACCTGTTCGCCGGTGATATTTATTGTGGACTTGGGGAAATGGCTTTACAGTACACCTCGGGGATTC
TGAAACATTCGTTGACTGGAGCGCTTCTGGCCTTTACCAACCCATCGACTAACTCTTACAGAAGGCTCGTTCCAGGATTC
GAAGCGCCTATATGTGCTGTTTTTGCTAAGGGGAACAGAAGCGCGGCTGTAAGAATTCCTGGTTACGTTAAAGATAAGCA
TAAAGCAAGGATAGAATTCAGGACGATAGACGCCAGCTGCAATCCATACTATGGAATAGCGGCCATGCTGCTTGCGGGTG
TGGACGGAGTCAAACAGAAACTCGACCATAAAGAGCTTGGATTTGGTCCCATCGAAACCAATATCTACAACATGAACGAC
GAATATAGAAATAAGATCCGCTTCTTTCCTTCAAGTTTAGAAGAATCATTAAGAGGTCTTAAGGAAGACAATGAGTTTTT
GAAACCCGGTTTTCCGGATGAGCTTATCAGGCAATGGATTTTAGTGAAGGAAGAAGAAGCGAGATATGTCAACAGCGTGC
CAAGTCCCGCGGAATACCAGCTTTATTTTGACCTTTGA

Upstream 100 bases:

>100_bases
AGCATATTTTACGCCTATAACGCATATTGGGTTTGAATAAAGGATGCAACAGGAATTAGAATTTTTGATGCAACCAATAA
AACTTGACAGGAGGTACGGT

Downstream 100 bases:

>100_bases
CAAATAACAAAGCTCTGCCGGGGGATTCCCGGGCAGAGCTTTGCTGTTGTCGTTCATTTCAAACGATCTCTATGAGGTTG
ATTTCAAATACGAGATCTTT

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKDSLRKYVGNIDFIDLKVIDIIGRVRHVILPGDRLTDELFEEGVGFDASNFGYSSIENSDMIMLPDPDTAFEDPFFERK
TLSFFCDVLRTEDRKPFEHYPRNVLRATLEKLKSIGAADAMLGPELEFHIFEGMSYRVSENQAYFKVDVAEGFWNSGEFS
NANVVERKMGYHRCPPADAMANFRNEAVMIMNSIGIPVKYHHHEVSSAQHEIEFRFQNALNSADSIILARYILQNLASRY
NLKVTFMPKPLFEEAGNGMHIHQYLVDGTGKNLFAGDIYCGLGEMALQYTSGILKHSLTGALLAFTNPSTNSYRRLVPGF
EAPICAVFAKGNRSAAVRIPGYVKDKHKARIEFRTIDASCNPYYGIAAMLLAGVDGVKQKLDHKELGFGPIETNIYNMND
EYRNKIRFFPSSLEESLRGLKEDNEFLKPGFPDELIRQWILVKEEEARYVNSVPSPAEYQLYFDL

Sequences:

>Translated_465_residues
MKDSLRKYVGNIDFIDLKVIDIIGRVRHVILPGDRLTDELFEEGVGFDASNFGYSSIENSDMIMLPDPDTAFEDPFFERK
TLSFFCDVLRTEDRKPFEHYPRNVLRATLEKLKSIGAADAMLGPELEFHIFEGMSYRVSENQAYFKVDVAEGFWNSGEFS
NANVVERKMGYHRCPPADAMANFRNEAVMIMNSIGIPVKYHHHEVSSAQHEIEFRFQNALNSADSIILARYILQNLASRY
NLKVTFMPKPLFEEAGNGMHIHQYLVDGTGKNLFAGDIYCGLGEMALQYTSGILKHSLTGALLAFTNPSTNSYRRLVPGF
EAPICAVFAKGNRSAAVRIPGYVKDKHKARIEFRTIDASCNPYYGIAAMLLAGVDGVKQKLDHKELGFGPIETNIYNMND
EYRNKIRFFPSSLEESLRGLKEDNEFLKPGFPDELIRQWILVKEEEARYVNSVPSPAEYQLYFDL
>Mature_465_residues
MKDSLRKYVGNIDFIDLKVIDIIGRVRHVILPGDRLTDELFEEGVGFDASNFGYSSIENSDMIMLPDPDTAFEDPFFERK
TLSFFCDVLRTEDRKPFEHYPRNVLRATLEKLKSIGAADAMLGPELEFHIFEGMSYRVSENQAYFKVDVAEGFWNSGEFS
NANVVERKMGYHRCPPADAMANFRNEAVMIMNSIGIPVKYHHHEVSSAQHEIEFRFQNALNSADSIILARYILQNLASRY
NLKVTFMPKPLFEEAGNGMHIHQYLVDGTGKNLFAGDIYCGLGEMALQYTSGILKHSLTGALLAFTNPSTNSYRRLVPGF
EAPICAVFAKGNRSAAVRIPGYVKDKHKARIEFRTIDASCNPYYGIAAMLLAGVDGVKQKLDHKELGFGPIETNIYNMND
EYRNKIRFFPSSLEESLRGLKEDNEFLKPGFPDELIRQWILVKEEEARYVNSVPSPAEYQLYFDL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=463, Percent_Identity=40.1727861771058, Blast_Score=340, Evalue=2e-94,
Organism=Escherichia coli, GI87081870, Length=410, Percent_Identity=27.5609756097561, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52854; Mature: 52854

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSLRKYVGNIDFIDLKVIDIIGRVRHVILPGDRLTDELFEEGVGFDASNFGYSSIENS
CCCHHHHHHCCCCEEEHHHHHHHHHHHEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCC
DMIMLPDPDTAFEDPFFERKTLSFFCDVLRTEDRKPFEHYPRNVLRATLEKLKSIGAADA
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
MLGPELEFHIFEGMSYRVSENQAYFKVDVAEGFWNSGEFSNANVVERKMGYHRCPPADAM
HCCCCEEEEEECCCEEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCHHHH
ANFRNEAVMIMNSIGIPVKYHHHEVSSAQHEIEFRFQNALNSADSIILARYILQNLASRY
HHCCCCEEEEEECCCCCEEECCCHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
NLKVTFMPKPLFEEAGNGMHIHQYLVDGTGKNLFAGDIYCGLGEMALQYTSGILKHSLTG
EEEEEECCCHHHHHCCCCEEEEEEEECCCCCCEEECCHHHCHHHHHHHHHHHHHHHHHCC
ALLAFTNPSTNSYRRLVPGFEAPICAVFAKGNRSAAVRIPGYVKDKHKARIEFRTIDASC
EEEEEECCCCCCHHHHCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCEEEEEEEECCCC
NPYYGIAAMLLAGVDGVKQKLDHKELGFGPIETNIYNMNDEYRNKIRFFPSSLEESLRGL
CCHHHHHHHHHHCCHHHHHHCCHHHCCCCCCCCEEECCCHHHHHHHHCCHHHHHHHHHCC
KEDNEFLKPGFPDELIRQWILVKEEEARYVNSVPSPAEYQLYFDL
CCCCCCCCCCCCHHHHHHHHEECCHHHHHHHCCCCCCEEEEEEEC
>Mature Secondary Structure
MKDSLRKYVGNIDFIDLKVIDIIGRVRHVILPGDRLTDELFEEGVGFDASNFGYSSIENS
CCCHHHHHHCCCCEEEHHHHHHHHHHHEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCC
DMIMLPDPDTAFEDPFFERKTLSFFCDVLRTEDRKPFEHYPRNVLRATLEKLKSIGAADA
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
MLGPELEFHIFEGMSYRVSENQAYFKVDVAEGFWNSGEFSNANVVERKMGYHRCPPADAM
HCCCCEEEEEECCCEEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCHHHH
ANFRNEAVMIMNSIGIPVKYHHHEVSSAQHEIEFRFQNALNSADSIILARYILQNLASRY
HHCCCCEEEEEECCCCCEEECCCHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
NLKVTFMPKPLFEEAGNGMHIHQYLVDGTGKNLFAGDIYCGLGEMALQYTSGILKHSLTG
EEEEEECCCHHHHHCCCCEEEEEEEECCCCCCEEECCHHHCHHHHHHHHHHHHHHHHHCC
ALLAFTNPSTNSYRRLVPGFEAPICAVFAKGNRSAAVRIPGYVKDKHKARIEFRTIDASC
EEEEEECCCCCCHHHHCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCEEEEEEEECCCC
NPYYGIAAMLLAGVDGVKQKLDHKELGFGPIETNIYNMNDEYRNKIRFFPSSLEESLRGL
CCHHHHHHHHHHCCHHHHHHCCHHHCCCCCCCCEEECCCHHHHHHHHCCHHHHHHHHHCC
KEDNEFLKPGFPDELIRQWILVKEEEARYVNSVPSPAEYQLYFDL
CCCCCCCCCCCCHHHHHHHHEECCHHHHHHHCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678591 [H]