Click here to switch to the map view.

The map label for this gene is mnmG [H]

Identifier: 239617771

GI number: 239617771

Start: 1486965

End: 1488869

Strand: Direct

Name: mnmG [H]

Synonym: Kole_1396

Alternate gene names: 239617771

Gene position: 1486965-1488869 (Clockwise)

Preceding gene: 239617764

Following gene: 239617773

Centisome position: 64.59

GC content: 44.88

Gene sequence:

>1905_bases
ATGTCCAAAACAATTAATGATTACAACTTTGATATAGTGGTTATTGGTGCGGGGCATGCCGGCATTGAAGCTGGACTCGC
CGCTGCAAAGATGGGGTTGAAAACACTTGTTTTAGCAATAAATCTCGATACTGTTGGCTGGGCTCCTTGCAACCCAGCTG
TTGGTGGGCCAGCCAAAGGGATTGTAGCTAGAGAGGTGGACGCTCTCGGGGGACAGATCGCAAAAACCACTGACGCCACG
ATGATAAACGTTAGAATGCTGAATACAAGCAAAGGACCAGCTGTTAGAGCTCTGAGAGCTCAGATCGATAAACATGAATA
CAGCATGGAAATGAAGAGAATACTGGAATCTCAAGAAAATCTTTTCATAAGGAGTGGAATAGCTTCAGAAATTCTCGTGG
AAAATGGGAAGGTTACAGGTGTTGTCACACATTTCGGTGTCGTGTACCGTTGCAGGGCGGCTGTTGTTACCTCCGGGACC
TTTCTCAGGGGAAAGATATTTGTAGGTCAAAAGGTTTTTGAGGCTGGAAGGCTCGGTGAATTTCCAGCGAAAGCATTGAG
TCAATCCCTTGAGACTCTTGGGTTTACGCTTTCGAGGTTCAAGACAGGAACTCCCGCAAGAATACAAGGGAAGAGCATAG
ATTTTTCAAAAATGGAGAGACAGGACACTTCTGATGAACCGCTGGCTTTTTCATATTTTGATGAACCCAGGGTTCTTCCC
AAAGATTACCCCTGCTGGTTGACCCACACAAATCCTTCTACTCATGCTGTCATAAGGCGTGATTTGAAATTTTCTCCCCT
TTACGGTGATGTGAAGCTGATCCAATCGATTGGGCCGCGTTATTGTCCTTCAATAGAAGACAAGGTTGTTAAATTCTCTT
CAAAAGAGAGCCATCAAGTCTTTGTTGAACCTGAAGGAAAGAATACAGATGAGTACTATTTGAACGGTCTTTCAACGAGT
CTTCCCTTTGAAACACAGGTGGAGATGATCCGTACTGTTCCCGGATTGGAGAATGCGGTTATTGTGAGGCCGGCTTACGC
TGTTGAATACGATTATGTCACTCCCGACCAGCTTTATCCGACTTTAGAATCAAAATTGGTGGAGAATCTGTATTTTGCCG
GTCAGGTTATCGGCACCAGCGGGTATGAAGAGGCCGCAGGCCTCGGGATCGTTGCGGGTATCAATGCGGCGGCAAAGCTT
CTTGGTATGGAGCAGTTTGTTCCAAAACGTTCGGAGTCGTACATAGGTGTGATGATAGATGATCTGGTTACAAAGGGAGT
CGATGAGCCTTATCGTTTGTTAACCTCCAGGGCGGAGTATAGGCTGTTGATAAGGCACGACAATGCTCACATCAGACTCA
GCAAGTATGGATACAAATACGGGTTGATTCCAAAATGGTTCTACGAAAAGGTTCTCGATTTAGAAAGACAGATAGAGGGA
GAGATAGAGAGATTAAACAATGTAGTCGTAAAGCCTTTGAGTACGCTCAATAATAAACTTGTGGCAGCTGGAACATCGCC
GATATACCAATCGACGCGTTTGTCTCAGTTGCTCAAAAGGCCCCAGGTGAGCTATGAAATACTCAAAGAGTTCGATCCTG
AACCGATAGAGGATCTTTTACTGAGGGAGCAAGTAGAAATACAGCTAAAATATGAAGGATATATATTAAGAATGGAGCAG
GAACTTAAACGCTTCGAAAGGTTGGAAATGGAGATAATTCCGCCAAAGGTCAATTATGATGAGGTGCCGAATCTCTCCAC
TGAGAGCCGTGAAAAGCTGAAAAAATTGAGGCCACGGTCCATTGGACAGGCCATGAGAATTCCGGGGATTACACCGGCAG
ACATAATGAACCTGTCAGTTTATCTAAAATCTTTGAGGAATATTGGCGAGGGGCAAAAAGATTGA

Upstream 100 bases:

>100_bases
ATTGATGGGCATCTCGAAAAGTTGTATCTGTGAAAGTAACTTTTTCCACGCTAACTCCTCCCCTCTGTTGTACAATTAAT
AAGAATAAAGGAGGGATGTT

Downstream 100 bases:

>100_bases
GGAACGGTTTTCACCGTTCCTCAATTCTTTAGAAGCTTTTCCAAAAAGATTTCCAATTCTTTCAGGCTTAAGCGCTTCCC
GTCTTTGAGCATTCTGAATG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 634; Mature: 633

Protein sequence:

>634_residues
MSKTINDYNFDIVVIGAGHAGIEAGLAAAKMGLKTLVLAINLDTVGWAPCNPAVGGPAKGIVAREVDALGGQIAKTTDAT
MINVRMLNTSKGPAVRALRAQIDKHEYSMEMKRILESQENLFIRSGIASEILVENGKVTGVVTHFGVVYRCRAAVVTSGT
FLRGKIFVGQKVFEAGRLGEFPAKALSQSLETLGFTLSRFKTGTPARIQGKSIDFSKMERQDTSDEPLAFSYFDEPRVLP
KDYPCWLTHTNPSTHAVIRRDLKFSPLYGDVKLIQSIGPRYCPSIEDKVVKFSSKESHQVFVEPEGKNTDEYYLNGLSTS
LPFETQVEMIRTVPGLENAVIVRPAYAVEYDYVTPDQLYPTLESKLVENLYFAGQVIGTSGYEEAAGLGIVAGINAAAKL
LGMEQFVPKRSESYIGVMIDDLVTKGVDEPYRLLTSRAEYRLLIRHDNAHIRLSKYGYKYGLIPKWFYEKVLDLERQIEG
EIERLNNVVVKPLSTLNNKLVAAGTSPIYQSTRLSQLLKRPQVSYEILKEFDPEPIEDLLLREQVEIQLKYEGYILRMEQ
ELKRFERLEMEIIPPKVNYDEVPNLSTESREKLKKLRPRSIGQAMRIPGITPADIMNLSVYLKSLRNIGEGQKD

Sequences:

>Translated_634_residues
MSKTINDYNFDIVVIGAGHAGIEAGLAAAKMGLKTLVLAINLDTVGWAPCNPAVGGPAKGIVAREVDALGGQIAKTTDAT
MINVRMLNTSKGPAVRALRAQIDKHEYSMEMKRILESQENLFIRSGIASEILVENGKVTGVVTHFGVVYRCRAAVVTSGT
FLRGKIFVGQKVFEAGRLGEFPAKALSQSLETLGFTLSRFKTGTPARIQGKSIDFSKMERQDTSDEPLAFSYFDEPRVLP
KDYPCWLTHTNPSTHAVIRRDLKFSPLYGDVKLIQSIGPRYCPSIEDKVVKFSSKESHQVFVEPEGKNTDEYYLNGLSTS
LPFETQVEMIRTVPGLENAVIVRPAYAVEYDYVTPDQLYPTLESKLVENLYFAGQVIGTSGYEEAAGLGIVAGINAAAKL
LGMEQFVPKRSESYIGVMIDDLVTKGVDEPYRLLTSRAEYRLLIRHDNAHIRLSKYGYKYGLIPKWFYEKVLDLERQIEG
EIERLNNVVVKPLSTLNNKLVAAGTSPIYQSTRLSQLLKRPQVSYEILKEFDPEPIEDLLLREQVEIQLKYEGYILRMEQ
ELKRFERLEMEIIPPKVNYDEVPNLSTESREKLKKLRPRSIGQAMRIPGITPADIMNLSVYLKSLRNIGEGQKD
>Mature_633_residues
SKTINDYNFDIVVIGAGHAGIEAGLAAAKMGLKTLVLAINLDTVGWAPCNPAVGGPAKGIVAREVDALGGQIAKTTDATM
INVRMLNTSKGPAVRALRAQIDKHEYSMEMKRILESQENLFIRSGIASEILVENGKVTGVVTHFGVVYRCRAAVVTSGTF
LRGKIFVGQKVFEAGRLGEFPAKALSQSLETLGFTLSRFKTGTPARIQGKSIDFSKMERQDTSDEPLAFSYFDEPRVLPK
DYPCWLTHTNPSTHAVIRRDLKFSPLYGDVKLIQSIGPRYCPSIEDKVVKFSSKESHQVFVEPEGKNTDEYYLNGLSTSL
PFETQVEMIRTVPGLENAVIVRPAYAVEYDYVTPDQLYPTLESKLVENLYFAGQVIGTSGYEEAAGLGIVAGINAAAKLL
GMEQFVPKRSESYIGVMIDDLVTKGVDEPYRLLTSRAEYRLLIRHDNAHIRLSKYGYKYGLIPKWFYEKVLDLERQIEGE
IERLNNVVVKPLSTLNNKLVAAGTSPIYQSTRLSQLLKRPQVSYEILKEFDPEPIEDLLLREQVEIQLKYEGYILRMEQE
LKRFERLEMEIIPPKVNYDEVPNLSTESREKLKKLRPRSIGQAMRIPGITPADIMNLSVYLKSLRNIGEGQKD

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=635, Percent_Identity=40.9448818897638, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI19882217, Length=660, Percent_Identity=39.3939393939394, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI183227703, Length=675, Percent_Identity=38.5185185185185, Blast_Score=427, Evalue=1e-119,
Organism=Escherichia coli, GI2367273, Length=620, Percent_Identity=47.258064516129, Blast_Score=569, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI17534255, Length=634, Percent_Identity=37.5394321766561, Blast_Score=407, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321202, Length=635, Percent_Identity=41.259842519685, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24658174, Length=630, Percent_Identity=40.6349206349206, Blast_Score=443, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 70947; Mature: 70815

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS01280 GIDA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTINDYNFDIVVIGAGHAGIEAGLAAAKMGLKTLVLAINLDTVGWAPCNPAVGGPAKG
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCCCCCCCC
IVAREVDALGGQIAKTTDATMINVRMLNTSKGPAVRALRAQIDKHEYSMEMKRILESQEN
HHHHHHHHHCCCEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LFIRSGIASEILVENGKVTGVVTHFGVVYRCRAAVVTSGTFLRGKIFVGQKVFEAGRLGE
EEEECCCCHHHHCCCCCEEEEHHHHHHHHHHHHHHEECCCEEEEEEEECHHHHHCCCCCC
FPAKALSQSLETLGFTLSRFKTGTPARIQGKSIDFSKMERQDTSDEPLAFSYFDEPRVLP
CHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCCCCCCCEEEEECCCCCCCC
KDYPCWLTHTNPSTHAVIRRDLKFSPLYGDVKLIQSIGPRYCPSIEDKVVKFSSKESHQV
CCCCEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHEECCCCCCCEE
FVEPEGKNTDEYYLNGLSTSLPFETQVEMIRTVPGLENAVIVRPAYAVEYDYVTPDQLYP
EECCCCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECEEEEEECCCCHHHHHH
TLESKLVENLYFAGQVIGTSGYEEAAGLGIVAGINAAAKLLGMEQFVPKRSESYIGVMID
HHHHHHHHHHHHHCEEECCCCCCHHCCCEEEECHHHHHHHHHHHHHCCCCCCCEEEEHHH
DLVTKGVDEPYRLLTSRAEYRLLIRHDNAHIRLSKYGYKYGLIPKWFYEKVLDLERQIEG
HHHHCCCCHHHHHHHCCCCEEEEEEECCCEEEEEECCCEECCCHHHHHHHHHHHHHHHHH
EIERLNNVVVKPLSTLNNKLVAAGTSPIYQSTRLSQLLKRPQVSYEILKEFDPEPIEDLL
HHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHH
LREQVEIQLKYEGYILRMEQELKRFERLEMEIIPPKVNYDEVPNLSTESREKLKKLRPRS
HHHCEEEEEEECCEEEEEHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCHH
IGQAMRIPGITPADIMNLSVYLKSLRNIGEGQKD
HCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKTINDYNFDIVVIGAGHAGIEAGLAAAKMGLKTLVLAINLDTVGWAPCNPAVGGPAKG
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCCCCCCCC
IVAREVDALGGQIAKTTDATMINVRMLNTSKGPAVRALRAQIDKHEYSMEMKRILESQEN
HHHHHHHHHCCCEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LFIRSGIASEILVENGKVTGVVTHFGVVYRCRAAVVTSGTFLRGKIFVGQKVFEAGRLGE
EEEECCCCHHHHCCCCCEEEEHHHHHHHHHHHHHHEECCCEEEEEEEECHHHHHCCCCCC
FPAKALSQSLETLGFTLSRFKTGTPARIQGKSIDFSKMERQDTSDEPLAFSYFDEPRVLP
CHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCCCCCCCEEEEECCCCCCCC
KDYPCWLTHTNPSTHAVIRRDLKFSPLYGDVKLIQSIGPRYCPSIEDKVVKFSSKESHQV
CCCCEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHEECCCCCCCEE
FVEPEGKNTDEYYLNGLSTSLPFETQVEMIRTVPGLENAVIVRPAYAVEYDYVTPDQLYP
EECCCCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECEEEEEECCCCHHHHHH
TLESKLVENLYFAGQVIGTSGYEEAAGLGIVAGINAAAKLLGMEQFVPKRSESYIGVMID
HHHHHHHHHHHHHCEEECCCCCCHHCCCEEEECHHHHHHHHHHHHHCCCCCCCEEEEHHH
DLVTKGVDEPYRLLTSRAEYRLLIRHDNAHIRLSKYGYKYGLIPKWFYEKVLDLERQIEG
HHHHCCCCHHHHHHHCCCCEEEEEEECCCEEEEEECCCEECCCHHHHHHHHHHHHHHHHH
EIERLNNVVVKPLSTLNNKLVAAGTSPIYQSTRLSQLLKRPQVSYEILKEFDPEPIEDLL
HHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHH
LREQVEIQLKYEGYILRMEQELKRFERLEMEIIPPKVNYDEVPNLSTESREKLKKLRPRS
HHHCEEEEEEECCEEEEEHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCHH
IGQAMRIPGITPADIMNLSVYLKSLRNIGEGQKD
HCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]