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The map label for this gene is murD [H]

Identifier: 239617755

GI number: 239617755

Start: 1466727

End: 1468076

Strand: Reverse

Name: murD [H]

Synonym: Kole_1380

Alternate gene names: 239617755

Gene position: 1468076-1466727 (Counterclockwise)

Preceding gene: 239617756

Following gene: 239617754

Centisome position: 63.77

GC content: 40.22

Gene sequence:

>1350_bases
ATGTACTCCGGAAAAGCAGGGATAATCGGCCTTGGCGAGTCGAATTATCGACTTGTGGAGTATCTTCTACAAAATTCACA
CTCAGAAATCTTTGTTTCAGAAAAAGGGAAACTTGATCTCGAAAAAAAAGAATTTCTAAAGACAAATAATATAGAGTTTG
AAGAAAGCGGTCATACTGAGAAACTCTACGATTGTGATTGGTTCATCCTGAGCCCGGGAATTTCTCCACACTCAGAAGTC
GGCGAGAGAATTCTCTCTTCGGGAAAACCCTTTACAACAGAACTGGAATTCGCCCTCGATAAACTCAAAAAACTCGAGCA
CGGTGTAATCGTTGGAATAACCGGTACCAATGGAAAATCAACAGTTACCACCATGGTTGGACATATCGCAAAAAAAAGTG
GGTTGAAAACCTTTATCGGAGGAAACCTGGGAACTCCACTAATAAGCTCTATCAATGAACGTTTTGATGTTTACGTAGTT
GAAGTGAGTTCCTTCCAACTCACGTGGTTCGGCAAAAAAGAAAAATACTTCCATCTATCTTCAATACTGAACATCGCCCA
GGACCACCTCGACTACCATAGGGACATGCGGGAGTATGCTCTCGCCAAACTCAAAATAATAGAGCTGACCTCCGGGTCTT
CCATAATAAACTCCGAAATACTCGAAAATTTTGGAGACCTACTGGTAGATAAAACCAAAAAACAAATTATTCCTTTTTCA
ACCAGAAAAGATAGCTTCTTCTGCCTGAAAGACGGTATAATGAGACTTCACAAATTATCCATATCTCTGGATAGTCTGAA
CTTTCCAGGAATTCATAATCAAGAAAACGCCGCGATAACCCTCGGACTCGCACAATTTCTGGGGATCCATGTCCACGACG
CTCTGGAAAAACTCCAGGATTACGCATTTCTCGACCACAGAATGCAAAAATGCGGGCAAATAAACGGCGTAACATTTATC
AACGACTCAAAAGCGACTAATGCCCACGCCGTTATCAATGCTTTGGAAAATTTCGACCTTGAAAAAGTTATAATCATCCT
ATCAGGAAAAGAAAAACGTGAAGATTACACCCCGTTGATCAAAAAAATATCCAGAGTAAAGCATATAATTTTTCTTGGTG
ATTCCTTAAAAAGCCTTATAGAAAGATTGAAAAAGAAAGGAATCACCTATGATGTGGTTCACACAATAGATGAGGCTCTC
AAAAAAGCCGTGGAGCTATCAAAAGCAGGTGACATTGTCTTGTTCAGTCCCGGTGGATCGAGTTTCGATCTGTATAAGAA
TTACAAAGAACGCGGAAAAGATTTCGTCAAAGCCTTTAACAACCTGATAAAAAGGGAAGAAGTGATCTGA

Upstream 100 bases:

>100_bases
TCGAACTTCTTGGCTGGAAAGAAGAAAAAATTGCGTTCCGTTTCTCAACACTCGCTTTATTAGTTTCTTTACTCGGAATA
ATCGGCTGGAGGGAATTATA

Downstream 100 bases:

>100_bases
TGAAACGGTCTTATCTTCTCCTAACCCTTTTTACCAGTGTTCTTTTGGTTCTTGGCTTTGTGTTTATATACAGCGCAGGG
ATCAGCATGGAAGCAAGACT

Product: UDP-N-acetylmuramoylalanine/D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MYSGKAGIIGLGESNYRLVEYLLQNSHSEIFVSEKGKLDLEKKEFLKTNNIEFEESGHTEKLYDCDWFILSPGISPHSEV
GERILSSGKPFTTELEFALDKLKKLEHGVIVGITGTNGKSTVTTMVGHIAKKSGLKTFIGGNLGTPLISSINERFDVYVV
EVSSFQLTWFGKKEKYFHLSSILNIAQDHLDYHRDMREYALAKLKIIELTSGSSIINSEILENFGDLLVDKTKKQIIPFS
TRKDSFFCLKDGIMRLHKLSISLDSLNFPGIHNQENAAITLGLAQFLGIHVHDALEKLQDYAFLDHRMQKCGQINGVTFI
NDSKATNAHAVINALENFDLEKVIIILSGKEKREDYTPLIKKISRVKHIIFLGDSLKSLIERLKKKGITYDVVHTIDEAL
KKAVELSKAGDIVLFSPGGSSFDLYKNYKERGKDFVKAFNNLIKREEVI

Sequences:

>Translated_449_residues
MYSGKAGIIGLGESNYRLVEYLLQNSHSEIFVSEKGKLDLEKKEFLKTNNIEFEESGHTEKLYDCDWFILSPGISPHSEV
GERILSSGKPFTTELEFALDKLKKLEHGVIVGITGTNGKSTVTTMVGHIAKKSGLKTFIGGNLGTPLISSINERFDVYVV
EVSSFQLTWFGKKEKYFHLSSILNIAQDHLDYHRDMREYALAKLKIIELTSGSSIINSEILENFGDLLVDKTKKQIIPFS
TRKDSFFCLKDGIMRLHKLSISLDSLNFPGIHNQENAAITLGLAQFLGIHVHDALEKLQDYAFLDHRMQKCGQINGVTFI
NDSKATNAHAVINALENFDLEKVIIILSGKEKREDYTPLIKKISRVKHIIFLGDSLKSLIERLKKKGITYDVVHTIDEAL
KKAVELSKAGDIVLFSPGGSSFDLYKNYKERGKDFVKAFNNLIKREEVI
>Mature_449_residues
MYSGKAGIIGLGESNYRLVEYLLQNSHSEIFVSEKGKLDLEKKEFLKTNNIEFEESGHTEKLYDCDWFILSPGISPHSEV
GERILSSGKPFTTELEFALDKLKKLEHGVIVGITGTNGKSTVTTMVGHIAKKSGLKTFIGGNLGTPLISSINERFDVYVV
EVSSFQLTWFGKKEKYFHLSSILNIAQDHLDYHRDMREYALAKLKIIELTSGSSIINSEILENFGDLLVDKTKKQIIPFS
TRKDSFFCLKDGIMRLHKLSISLDSLNFPGIHNQENAAITLGLAQFLGIHVHDALEKLQDYAFLDHRMQKCGQINGVTFI
NDSKATNAHAVINALENFDLEKVIIILSGKEKREDYTPLIKKISRVKHIIFLGDSLKSLIERLKKKGITYDVVHTIDEAL
KKAVELSKAGDIVLFSPGGSSFDLYKNYKERGKDFVKAFNNLIKREEVI

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=388, Percent_Identity=28.8659793814433, Blast_Score=145, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 50552; Mature: 50552

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSGKAGIIGLGESNYRLVEYLLQNSHSEIFVSEKGKLDLEKKEFLKTNNIEFEESGHTE
CCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCC
KLYDCDWFILSPGISPHSEVGERILSSGKPFTTELEFALDKLKKLEHGVIVGITGTNGKS
EEECCCEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCH
TVTTMVGHIAKKSGLKTFIGGNLGTPLISSINERFDVYVVEVSSFQLTWFGKKEKYFHLS
HHHHHHHHHHHHCCCCEEECCCCCCHHHHHHCCCCEEEEEEEEEEEEEEECCCCCHHHHH
SILNIAQDHLDYHRDMREYALAKLKIIELTSGSSIINSEILENFGDLLVDKTKKQIIPFS
HHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHCCCCC
TRKDSFFCLKDGIMRLHKLSISLDSLNFPGIHNQENAAITLGLAQFLGIHVHDALEKLQD
CCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
YAFLDHRMQKCGQINGVTFINDSKATNAHAVINALENFDLEKVIIILSGKEKREDYTPLI
HHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHH
KKISRVKHIIFLGDSLKSLIERLKKKGITYDVVHTIDEALKKAVELSKAGDIVLFSPGGS
HHHHHHHEEEEECHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
SFDLYKNYKERGKDFVKAFNNLIKREEVI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYSGKAGIIGLGESNYRLVEYLLQNSHSEIFVSEKGKLDLEKKEFLKTNNIEFEESGHTE
CCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCC
KLYDCDWFILSPGISPHSEVGERILSSGKPFTTELEFALDKLKKLEHGVIVGITGTNGKS
EEECCCEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCH
TVTTMVGHIAKKSGLKTFIGGNLGTPLISSINERFDVYVVEVSSFQLTWFGKKEKYFHLS
HHHHHHHHHHHHCCCCEEECCCCCCHHHHHHCCCCEEEEEEEEEEEEEEECCCCCHHHHH
SILNIAQDHLDYHRDMREYALAKLKIIELTSGSSIINSEILENFGDLLVDKTKKQIIPFS
HHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHCCCCC
TRKDSFFCLKDGIMRLHKLSISLDSLNFPGIHNQENAAITLGLAQFLGIHVHDALEKLQD
CCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
YAFLDHRMQKCGQINGVTFINDSKATNAHAVINALENFDLEKVIIILSGKEKREDYTPLI
HHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHH
KKISRVKHIIFLGDSLKSLIERLKKKGITYDVVHTIDEALKKAVELSKAGDIVLFSPGGS
HHHHHHHEEEEECHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
SFDLYKNYKERGKDFVKAFNNLIKREEVI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA