Click here to switch to the map view.

The map label for this gene is rihA [H]

Identifier: 239617633

GI number: 239617633

Start: 1330648

End: 1331571

Strand: Direct

Name: rihA [H]

Synonym: Kole_1254

Alternate gene names: 239617633

Gene position: 1330648-1331571 (Clockwise)

Preceding gene: 239617632

Following gene: 239617634

Centisome position: 57.8

GC content: 37.01

Gene sequence:

>924_bases
ATGAAACGTAAGATTATTTTGGATTGTGATCCTGGTCACGATGATGCTGTCGCTATATTGCTGGCAGGAATCGCAGAAGA
GATTGAATTACTAGGTATAGTATCTGTAGCCGGTAATTCATATATAGAGAATACAACAAGAAATGCCCTTATTTTGGCGG
AAATGGCAAAGCTGGATGTGCCAGTATTTAGAGGATCTTCAAAACCATTGTTGAGAAAGCAAATTGTGGCTCCAGATATT
CATGGTGAAAGTGGTTTGGAAGGAGCAAATCTTCCACTACCTACTAAAAAAGCTGAAGAAAAGAATTACTTGGAGTTTAT
GGCAGAAACTGTTGAAAAATTCCCCAATGAAATTACATTCGTAGCTGTCGGACCGCTAACAAACATAGCTAAATTTGTTC
TTAACTATCCTCATTTAGTTTCCAAAGTGAAAGAATTGGTAATTATGGGTGGGGGAATAAAGTTTGGAAACGTAACTCCA
AGGGCTGAATTCAATATATACGCCGATCCTGAAGCTGCGCAAATAGTTTTCAACGCGGGATTCAATTTAACTGTTTTCCC
GCTCGACGTGACTCACCAGGCAAAAATATATATGAAAGAGATAAAAGAGATGCAGAATTTTAGATCCGAAATAACTTCTA
AAATGGGATTGTTATTAGAATTCTTCCACCAAACGTATTACGATATATTTAAAATAGAAGGGGCTCCTTTGCATGATCCA
TGTACCATAGCCTATTTAATAAACCCGGATATATTCGAATTCGATGAATTTTATGCCCAGGTAGAAACAAAAGGTGAATT
AACATACGGTGAAACAGTTGTCGATTATTGGCACCTGGAGGCCCCGAATTCAAAATGGGCGCTGAAGGTTGATAGAGAGA
AGTTCATTGAAATACTTTTTGATAATTTAAAGAAATACAATTAA

Upstream 100 bases:

>100_bases
AATGCCCTTCTTAATAACAGTCGGCGCTCTGGCATTCTATGCGTGGAACAAACTAAGAGAAGGAAAAATCAAATTCTTCT
GAATATAAGGTGGTGTGGGT

Downstream 100 bases:

>100_bases
TTTAATTCTAAATTCTATTCTAAAAGAAAGGAGAGAGGTGAAACCATGGAAGATCCAAGATACCACATAAGAATAGACGA
TCCAGAAAAAGTAGGAAAAT

Product: Uridine nucleosidase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKRKIILDCDPGHDDAVAILLAGIAEEIELLGIVSVAGNSYIENTTRNALILAEMAKLDVPVFRGSSKPLLRKQIVAPDI
HGESGLEGANLPLPTKKAEEKNYLEFMAETVEKFPNEITFVAVGPLTNIAKFVLNYPHLVSKVKELVIMGGGIKFGNVTP
RAEFNIYADPEAAQIVFNAGFNLTVFPLDVTHQAKIYMKEIKEMQNFRSEITSKMGLLLEFFHQTYYDIFKIEGAPLHDP
CTIAYLINPDIFEFDEFYAQVETKGELTYGETVVDYWHLEAPNSKWALKVDREKFIEILFDNLKKYN

Sequences:

>Translated_307_residues
MKRKIILDCDPGHDDAVAILLAGIAEEIELLGIVSVAGNSYIENTTRNALILAEMAKLDVPVFRGSSKPLLRKQIVAPDI
HGESGLEGANLPLPTKKAEEKNYLEFMAETVEKFPNEITFVAVGPLTNIAKFVLNYPHLVSKVKELVIMGGGIKFGNVTP
RAEFNIYADPEAAQIVFNAGFNLTVFPLDVTHQAKIYMKEIKEMQNFRSEITSKMGLLLEFFHQTYYDIFKIEGAPLHDP
CTIAYLINPDIFEFDEFYAQVETKGELTYGETVVDYWHLEAPNSKWALKVDREKFIEILFDNLKKYN
>Mature_307_residues
MKRKIILDCDPGHDDAVAILLAGIAEEIELLGIVSVAGNSYIENTTRNALILAEMAKLDVPVFRGSSKPLLRKQIVAPDI
HGESGLEGANLPLPTKKAEEKNYLEFMAETVEKFPNEITFVAVGPLTNIAKFVLNYPHLVSKVKELVIMGGGIKFGNVTP
RAEFNIYADPEAAQIVFNAGFNLTVFPLDVTHQAKIYMKEIKEMQNFRSEITSKMGLLLEFFHQTYYDIFKIEGAPLHDP
CTIAYLINPDIFEFDEFYAQVETKGELTYGETVVDYWHLEAPNSKWALKVDREKFIEILFDNLKKYN

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=310, Percent_Identity=44.5161290322581, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1788486, Length=307, Percent_Identity=38.7622149837134, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1786213, Length=300, Percent_Identity=38.6666666666667, Blast_Score=178, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI32565474, Length=198, Percent_Identity=31.8181818181818, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17565698, Length=312, Percent_Identity=27.5641025641026, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320608, Length=260, Percent_Identity=40.7692307692308, Blast_Score=161, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24583915, Length=336, Percent_Identity=28.5714285714286, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24641839, Length=296, Percent_Identity=30.0675675675676, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24641837, Length=297, Percent_Identity=26.5993265993266, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 34655; Mature: 34655

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKIILDCDPGHDDAVAILLAGIAEEIELLGIVSVAGNSYIENTTRNALILAEMAKLDV
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCHHHCCCCCEEEEEHHHHCCC
PVFRGSSKPLLRKQIVAPDIHGESGLEGANLPLPTKKAEEKNYLEFMAETVEKFPNEITF
CEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
VAVGPLTNIAKFVLNYPHLVSKVKELVIMGGGIKFGNVTPRAEFNIYADPEAAQIVFNAG
EEECCHHHHHHHHHCCHHHHHHHHHHEEECCCEEECCCCCCCEEEEEECCCCEEEEEECC
FNLTVFPLDVTHQAKIYMKEIKEMQNFRSEITSKMGLLLEFFHQTYYDIFKIEGAPLHDP
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
CTIAYLINPDIFEFDEFYAQVETKGELTYGETVVDYWHLEAPNSKWALKVDREKFIEILF
EEEEEEECCCCHHHHHHHHHHHCCCCEEECCEEEEEEEEECCCCCEEEEECHHHHHHHHH
DNLKKYN
HHHHCCC
>Mature Secondary Structure
MKRKIILDCDPGHDDAVAILLAGIAEEIELLGIVSVAGNSYIENTTRNALILAEMAKLDV
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCHHHCCCCCEEEEEHHHHCCC
PVFRGSSKPLLRKQIVAPDIHGESGLEGANLPLPTKKAEEKNYLEFMAETVEKFPNEITF
CEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
VAVGPLTNIAKFVLNYPHLVSKVKELVIMGGGIKFGNVTPRAEFNIYADPEAAQIVFNAG
EEECCHHHHHHHHHCCHHHHHHHHHHEEECCCEEECCCCCCCEEEEEECCCCEEEEEECC
FNLTVFPLDVTHQAKIYMKEIKEMQNFRSEITSKMGLLLEFFHQTYYDIFKIEGAPLHDP
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
CTIAYLINPDIFEFDEFYAQVETKGELTYGETVVDYWHLEAPNSKWALKVDREKFIEILF
EEEEEEECCCCHHHHHHHHHHHCCCCEEECCEEEEEEEEECCCCCEEEEECHHHHHHHHH
DNLKKYN
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA