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The map label for this gene is aglA [H]

Identifier: 239617556

GI number: 239617556

Start: 1242769

End: 1244373

Strand: Direct

Name: aglA [H]

Synonym: Kole_1174

Alternate gene names: 239617556

Gene position: 1242769-1244373 (Clockwise)

Preceding gene: 239617555

Following gene: 239617566

Centisome position: 53.98

GC content: 41.18

Gene sequence:

>1605_bases
TTGAAAGAACCATGGTGGAAAGGTGCAGTAATTTATCAAGTTTATCCACGTAGTTTTAAGGACAGTAACAATGATGGAAT
TGGAGATTTAAAAGGAGTCTCATCAAAACTGGATTATATAGCTGATCTTGGGGTCGACGCCATTTGGCTTTCCCCTATTT
ATAAATCACCGATGAAGGACTTTGGGTATGATATTTCAGATTACTATGCTATTGACCCGATTTTCGGAACAATGGAAGAT
TTCGATGAACTATTGGAAGAAGCCCACAAGAGAGGATTAAAGGTTATTTTAGATATGGTCTTGAATCATACCTCAGATCA
ACATCCATGGTTCATTGAATCGAGGTCTTCAAGGGATAATCCAAAAGCTGATTGGTACATATGGGTAGATGGAGAGAAGG
GAACACCACCGAACAATTGGCAGTCGTACTTTGGTGGTAGTGCCTGGAATTGGGATGAGACACGAAAACAGTACTATCTC
TGCTTATTTACAAAGGAACAGCCAGATCTCAATTGGAGAAACCCTGAGGTGAAGAAAGCTGTTTTTGATGTAGTGAGATT
TTGGCTTGAAAAAGGTGTTGATGGTTTCAGATTGGATGTCGTGAACCTTTACTACAAAGATGCAAAGCTTAGGAACAACC
CCAGGAAAAAGAGAAGAACGGAAATCGAGTTCGAGAACTACTACAATATCTTCACCCGTGATAGGCCCGAGACTTTGCTC
GCTGTCGAAGAACTCCAGGAGATTGTTGATAGCTTTGGTGACAGAGTTACGATTGGTGAAGTTGCAACTGATTTAGGGGT
TATTCAGTATTATGAATACACAAAGCCCGGTAGATTAAATCTGGCTTTCAATTTTGAATTTAAAGATGTAAACATTTTCA
GTGCACGTGCTTTTGGAGATGTGATTGATCTTACCGAGCGTATCTTTGGTGAGGTTGCTTGGCCCAGCTATGTGCTGGGA
AACCATGATTCCCCCAGGTTTATTTCCCGTTTTTCGAATGGAAAGGACGATGTTCAACGTGCAAGGGTGCTGGCAGCTAT
GTTACTAACTGTCAGGGGAACCCCCTTCCTTTATGCAGGTGAAGAAATTGGGATGACAGAAGGAGATATACCTTACGAGA
AGCTTCAAGATCCATTGGGAGTAAACCTTTGGCCGAAGCATAAAGGAAGAGATGGTTGTCGTACACCGATGCAGTGGGAC
GACAGTGAATTTGCAGGGTTCTCTACTGTGGAACCATGGTTACCCGTCAATGAAAACAAGAACGAAGTAAATGTTGAACA
TGAAGCCAGAGACCCGAATTCAATGTTGAATTATTATAAAGAACTTCTAAGGTTGCGAAAAGAATCAAACGCATTGAAAT
TTGGTGATTACAGGAAACTCGAAACTAGTAATACCGAGGTTCTTGCTTATCTCCGCGAATATGAGGATGAAAGAAAGATT
GTGATCTTGAATTTCAATGATCAAGAAGTTGGACTTGAATTGAAGGGCACAGATTTCAAGGGGTTTTCTGTTCAATTTGG
TACGCATAGGCAAGAGGGAATTTTGAGCTTAGAACTAACGCTTGCCCCTCTGGAAATTATAATCGGGGAAGTTTTAGGTC
CATAG

Upstream 100 bases:

>100_bases
TTAATAATAATGGTCCTTTTCCTTCAAAGGTGGATAATTGCCGGGCTTACTGCTGGAGCTATCAAAGATTGATAAATTCT
CATCGGAAGGAGAGATCATT

Downstream 100 bases:

>100_bases
TACTGTTGAAACCAAAACGCCTCCCGCAGAGGGAGGCGTTTTTTCTAGACTGACTAGGGAGGTTGGCGATATATCAAGAA
CTTGAAGGTGTCGTGTAATC

Product: alpha amylase catalytic region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MKEPWWKGAVIYQVYPRSFKDSNNDGIGDLKGVSSKLDYIADLGVDAIWLSPIYKSPMKDFGYDISDYYAIDPIFGTMED
FDELLEEAHKRGLKVILDMVLNHTSDQHPWFIESRSSRDNPKADWYIWVDGEKGTPPNNWQSYFGGSAWNWDETRKQYYL
CLFTKEQPDLNWRNPEVKKAVFDVVRFWLEKGVDGFRLDVVNLYYKDAKLRNNPRKKRRTEIEFENYYNIFTRDRPETLL
AVEELQEIVDSFGDRVTIGEVATDLGVIQYYEYTKPGRLNLAFNFEFKDVNIFSARAFGDVIDLTERIFGEVAWPSYVLG
NHDSPRFISRFSNGKDDVQRARVLAAMLLTVRGTPFLYAGEEIGMTEGDIPYEKLQDPLGVNLWPKHKGRDGCRTPMQWD
DSEFAGFSTVEPWLPVNENKNEVNVEHEARDPNSMLNYYKELLRLRKESNALKFGDYRKLETSNTEVLAYLREYEDERKI
VILNFNDQEVGLELKGTDFKGFSVQFGTHRQEGILSLELTLAPLEIIIGEVLGP

Sequences:

>Translated_534_residues
MKEPWWKGAVIYQVYPRSFKDSNNDGIGDLKGVSSKLDYIADLGVDAIWLSPIYKSPMKDFGYDISDYYAIDPIFGTMED
FDELLEEAHKRGLKVILDMVLNHTSDQHPWFIESRSSRDNPKADWYIWVDGEKGTPPNNWQSYFGGSAWNWDETRKQYYL
CLFTKEQPDLNWRNPEVKKAVFDVVRFWLEKGVDGFRLDVVNLYYKDAKLRNNPRKKRRTEIEFENYYNIFTRDRPETLL
AVEELQEIVDSFGDRVTIGEVATDLGVIQYYEYTKPGRLNLAFNFEFKDVNIFSARAFGDVIDLTERIFGEVAWPSYVLG
NHDSPRFISRFSNGKDDVQRARVLAAMLLTVRGTPFLYAGEEIGMTEGDIPYEKLQDPLGVNLWPKHKGRDGCRTPMQWD
DSEFAGFSTVEPWLPVNENKNEVNVEHEARDPNSMLNYYKELLRLRKESNALKFGDYRKLETSNTEVLAYLREYEDERKI
VILNFNDQEVGLELKGTDFKGFSVQFGTHRQEGILSLELTLAPLEIIIGEVLGP
>Mature_534_residues
MKEPWWKGAVIYQVYPRSFKDSNNDGIGDLKGVSSKLDYIADLGVDAIWLSPIYKSPMKDFGYDISDYYAIDPIFGTMED
FDELLEEAHKRGLKVILDMVLNHTSDQHPWFIESRSSRDNPKADWYIWVDGEKGTPPNNWQSYFGGSAWNWDETRKQYYL
CLFTKEQPDLNWRNPEVKKAVFDVVRFWLEKGVDGFRLDVVNLYYKDAKLRNNPRKKRRTEIEFENYYNIFTRDRPETLL
AVEELQEIVDSFGDRVTIGEVATDLGVIQYYEYTKPGRLNLAFNFEFKDVNIFSARAFGDVIDLTERIFGEVAWPSYVLG
NHDSPRFISRFSNGKDDVQRARVLAAMLLTVRGTPFLYAGEEIGMTEGDIPYEKLQDPLGVNLWPKHKGRDGCRTPMQWD
DSEFAGFSTVEPWLPVNENKNEVNVEHEARDPNSMLNYYKELLRLRKESNALKFGDYRKLETSNTEVLAYLREYEDERKI
VILNFNDQEVGLELKGTDFKGFSVQFGTHRQEGILSLELTLAPLEIIIGEVLGP

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=513, Percent_Identity=37.6218323586745, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI65506891, Length=109, Percent_Identity=34.8623853211009, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI61744479, Length=109, Percent_Identity=34.8623853211009, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI61744483, Length=109, Percent_Identity=34.8623853211009, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI61744481, Length=109, Percent_Identity=34.8623853211009, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI61744475, Length=109, Percent_Identity=34.8623853211009, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI61744477, Length=109, Percent_Identity=34.8623853211009, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1790687, Length=511, Percent_Identity=40.1174168297456, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI1786604, Length=477, Percent_Identity=24.3186582809224, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789995, Length=91, Percent_Identity=35.1648351648352, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32565753, Length=505, Percent_Identity=26.5346534653465, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI25147709, Length=482, Percent_Identity=26.1410788381743, Blast_Score=134, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321726, Length=586, Percent_Identity=37.8839590443686, Blast_Score=352, Evalue=8e-98,
Organism=Saccharomyces cerevisiae, GI6322241, Length=586, Percent_Identity=37.0307167235495, Blast_Score=347, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6322021, Length=586, Percent_Identity=37.0307167235495, Blast_Score=347, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6324416, Length=586, Percent_Identity=37.2013651877133, Blast_Score=347, Evalue=3e-96,
Organism=Saccharomyces cerevisiae, GI6321731, Length=548, Percent_Identity=39.2335766423358, Blast_Score=342, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6319776, Length=548, Percent_Identity=39.2335766423358, Blast_Score=341, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6322245, Length=579, Percent_Identity=35.9240069084629, Blast_Score=328, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24583747, Length=520, Percent_Identity=43.0769230769231, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24583749, Length=520, Percent_Identity=43.0769230769231, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24583745, Length=508, Percent_Identity=41.9291338582677, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24586593, Length=509, Percent_Identity=42.0432220039293, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24586591, Length=469, Percent_Identity=41.36460554371, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI45549022, Length=572, Percent_Identity=37.7622377622378, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI221330053, Length=558, Percent_Identity=37.4551971326165, Blast_Score=329, Evalue=4e-90,
Organism=Drosophila melanogaster, GI24586599, Length=521, Percent_Identity=38.5796545105566, Blast_Score=328, Evalue=4e-90,
Organism=Drosophila melanogaster, GI24586589, Length=528, Percent_Identity=39.9621212121212, Blast_Score=325, Evalue=6e-89,
Organism=Drosophila melanogaster, GI24586587, Length=554, Percent_Identity=37.0036101083032, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24586597, Length=520, Percent_Identity=37.3076923076923, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI281360393, Length=456, Percent_Identity=39.9122807017544, Blast_Score=273, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 62040; Mature: 62040

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEPWWKGAVIYQVYPRSFKDSNNDGIGDLKGVSSKLDYIADLGVDAIWLSPIYKSPMKD
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHCHHHH
FGYDISDYYAIDPIFGTMEDFDELLEEAHKRGLKVILDMVLNHTSDQHPWFIESRSSRDN
HCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCC
PKADWYIWVDGEKGTPPNNWQSYFGGSAWNWDETRKQYYLCLFTKEQPDLNWRNPEVKKA
CCCCEEEEEECCCCCCCCCHHHHCCCCCCCHHHHCCEEEEEEEECCCCCCCCCCHHHHHH
VFDVVRFWLEKGVDGFRLDVVNLYYKDAKLRNNPRKKRRTEIEFENYYNIFTRDRPETLL
HHHHHHHHHHCCCCCEEEEEEEEEEECHHHCCCCHHHHCCCCCHHHHEEEEECCCCHHHH
AVEELQEIVDSFGDRVTIGEVATDLGVIQYYEYTKPGRLNLAFNFEFKDVNIFSARAFGD
HHHHHHHHHHHHCCEEEEHHHHHHHHHHEEEECCCCCEEEEEEEEEEECEEEEEHHHHHH
VIDLTERIFGEVAWPSYVLGNHDSPRFISRFSNGKDDVQRARVLAAMLLTVRGTPFLYAG
HHHHHHHHHHCCCCCCCEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEC
EEIGMTEGDIPYEKLQDPLGVNLWPKHKGRDGCRTPMQWDDSEFAGFSTVEPWLPVNENK
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NEVNVEHEARDPNSMLNYYKELLRLRKESNALKFGDYRKLETSNTEVLAYLREYEDERKI
CEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHCCCCCEE
VILNFNDQEVGLELKGTDFKGFSVQFGTHRQEGILSLELTLAPLEIIIGEVLGP
EEEEECCCEECEEEECCCCCCEEEEECCCCCCCEEEEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEPWWKGAVIYQVYPRSFKDSNNDGIGDLKGVSSKLDYIADLGVDAIWLSPIYKSPMKD
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHCHHHH
FGYDISDYYAIDPIFGTMEDFDELLEEAHKRGLKVILDMVLNHTSDQHPWFIESRSSRDN
HCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCC
PKADWYIWVDGEKGTPPNNWQSYFGGSAWNWDETRKQYYLCLFTKEQPDLNWRNPEVKKA
CCCCEEEEEECCCCCCCCCHHHHCCCCCCCHHHHCCEEEEEEEECCCCCCCCCCHHHHHH
VFDVVRFWLEKGVDGFRLDVVNLYYKDAKLRNNPRKKRRTEIEFENYYNIFTRDRPETLL
HHHHHHHHHHCCCCCEEEEEEEEEEECHHHCCCCHHHHCCCCCHHHHEEEEECCCCHHHH
AVEELQEIVDSFGDRVTIGEVATDLGVIQYYEYTKPGRLNLAFNFEFKDVNIFSARAFGD
HHHHHHHHHHHHCCEEEEHHHHHHHHHHEEEECCCCCEEEEEEEEEEECEEEEEHHHHHH
VIDLTERIFGEVAWPSYVLGNHDSPRFISRFSNGKDDVQRARVLAAMLLTVRGTPFLYAG
HHHHHHHHHHCCCCCCCEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEC
EEIGMTEGDIPYEKLQDPLGVNLWPKHKGRDGCRTPMQWDDSEFAGFSTVEPWLPVNENK
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NEVNVEHEARDPNSMLNYYKELLRLRKESNALKFGDYRKLETSNTEVLAYLREYEDERKI
CEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHCCCCCEE
VILNFNDQEVGLELKGTDFKGFSVQFGTHRQEGILSLELTLAPLEIIIGEVLGP
EEEEECCCEECEEEECCCCCCEEEEECCCCCCCEEEEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]