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The map label for this gene is icaA [H]

Identifier: 239617492

GI number: 239617492

Start: 1176583

End: 1177716

Strand: Direct

Name: icaA [H]

Synonym: Kole_1108

Alternate gene names: 239617492

Gene position: 1176583-1177716 (Clockwise)

Preceding gene: 239617491

Following gene: 239617493

Centisome position: 51.11

GC content: 38.89

Gene sequence:

>1134_bases
TTGGTAAGCTTTGTGTACGGTGTGATCATAGGATTTGTATTTGCGTTTATTGGGTACGTGAAAAATCACTTCACAAAAAA
GCTTCTGGACGTTAATTATTCAGACGATGTTGATTACTCTGTTGTGGCTGTCATTGCCGCAAAGAATGAAGAAAGTGTTA
TAGAAAATACCGTTCGCAATTTGTTGACTAATTCCCCCCGATCTTTTCGGGTAATTGTTGTTGACGATAAATCCACGGAT
TCCACATATAAAATCCTGAGCGAGTTGTCAAGGGAATTTCCAAATTTGACCGTTGTTCGTAACACTGGTGCTCCAGGTAA
ATCTGAAGCTTTGAATGTTGCTCTTGATCTGGTCAAAGAGGATATTGTACTTTTCCTTGATGCTGACGCGCGTGTGGATT
GGGATTTTATTAAGGAGTATTCCAAACTCTTCCGCTCCCCTGAAATTAATGTTGTATTCACAGACTTCGAATCATATAAT
CAATCACGTACCCTGGCAGTTATTCTCCAGGATCTTTATTTTTCCTTTATAAAAGCATTCGTATATTCTGGACTGTTTTC
ACCAACGATCTTCATGAACAGCGGCGTTTTCATCAGGAGAAAGGTTTTCGATATTGTTGGAAAGTTCGATCCACAAACTC
TCGTGGATGATTTTGATTTAGCTCTTAGGCTCTGGAAGAGGAAACTCAAAGTGAAATTCGTCAGAGGCAAAAGGTGCAAG
ATACAGTATGCTTTCAGACTACAAGATTTATTCAAACAACACTGTCGCTGGTACATTGGGGGCATCAAAAAAATCTTTGA
ACAGTTCTCTGAGGGTTACTATCTCGGTATTATAGCGATAGTAGCAATTGGAGCATTTGTGCTTTTTCCTATTTTGATGC
TTGTATTAGCATACTTGCTAAAGGCTGATTATCTACTCAGCACAGTTATGCCGTTGTTCTGGGGCATTTTATACTCAAGT
TCTTTGTTCAGCTATCTTTTCCACGATGGCCACAGGCCAAAAGAAATACTTATTAACACCATTATAGGTGTTCCTGTAAT
GTACGCTTTCTTCCAAATCGCTATTCTGGTTTCTTTTGTCAGGTCTTTTGAAAAGAAATCTACGTGGTATAAGGTCAAGC
GAGAAAAGATATGA

Upstream 100 bases:

>100_bases
AGTAGGGTTTATTTGCCTAAAAAACATTATGTATGCTTGAAGCATACAACTTTTTATGTCTGATATTATGTATAATGATA
TTGATTGGTGGTGAAGTGTT

Downstream 100 bases:

>100_bases
AGGCCATGGGTTTACTTAAGGGTGTGTGAAAAAATGGCTCCATTGATTACGATAATCGTACCGGTCTTCAACGAAGAATT
CTTTTTGGAAGATACATTGA

Product: glycosyl transferase family 2

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MVSFVYGVIIGFVFAFIGYVKNHFTKKLLDVNYSDDVDYSVVAVIAAKNEESVIENTVRNLLTNSPRSFRVIVVDDKSTD
STYKILSELSREFPNLTVVRNTGAPGKSEALNVALDLVKEDIVLFLDADARVDWDFIKEYSKLFRSPEINVVFTDFESYN
QSRTLAVILQDLYFSFIKAFVYSGLFSPTIFMNSGVFIRRKVFDIVGKFDPQTLVDDFDLALRLWKRKLKVKFVRGKRCK
IQYAFRLQDLFKQHCRWYIGGIKKIFEQFSEGYYLGIIAIVAIGAFVLFPILMLVLAYLLKADYLLSTVMPLFWGILYSS
SLFSYLFHDGHRPKEILINTIIGVPVMYAFFQIAILVSFVRSFEKKSTWYKVKREKI

Sequences:

>Translated_377_residues
MVSFVYGVIIGFVFAFIGYVKNHFTKKLLDVNYSDDVDYSVVAVIAAKNEESVIENTVRNLLTNSPRSFRVIVVDDKSTD
STYKILSELSREFPNLTVVRNTGAPGKSEALNVALDLVKEDIVLFLDADARVDWDFIKEYSKLFRSPEINVVFTDFESYN
QSRTLAVILQDLYFSFIKAFVYSGLFSPTIFMNSGVFIRRKVFDIVGKFDPQTLVDDFDLALRLWKRKLKVKFVRGKRCK
IQYAFRLQDLFKQHCRWYIGGIKKIFEQFSEGYYLGIIAIVAIGAFVLFPILMLVLAYLLKADYLLSTVMPLFWGILYSS
SLFSYLFHDGHRPKEILINTIIGVPVMYAFFQIAILVSFVRSFEKKSTWYKVKREKI
>Mature_377_residues
MVSFVYGVIIGFVFAFIGYVKNHFTKKLLDVNYSDDVDYSVVAVIAAKNEESVIENTVRNLLTNSPRSFRVIVVDDKSTD
STYKILSELSREFPNLTVVRNTGAPGKSEALNVALDLVKEDIVLFLDADARVDWDFIKEYSKLFRSPEINVVFTDFESYN
QSRTLAVILQDLYFSFIKAFVYSGLFSPTIFMNSGVFIRRKVFDIVGKFDPQTLVDDFDLALRLWKRKLKVKFVRGKRCK
IQYAFRLQDLFKQHCRWYIGGIKKIFEQFSEGYYLGIIAIVAIGAFVLFPILMLVLAYLLKADYLLSTVMPLFWGILYSS
SLFSYLFHDGHRPKEILINTIIGVPVMYAFFQIAILVSFVRSFEKKSTWYKVKREKI

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=227, Percent_Identity=26.8722466960352, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 43605; Mature: 43605

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSFVYGVIIGFVFAFIGYVKNHFTKKLLDVNYSDDVDYSVVAVIAAKNEESVIENTVRN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHHH
LLTNSPRSFRVIVVDDKSTDSTYKILSELSREFPNLTVVRNTGAPGKSEALNVALDLVKE
HHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHH
DIVLFLDADARVDWDFIKEYSKLFRSPEINVVFTDFESYNQSRTLAVILQDLYFSFIKAF
CEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECHHHCCCCHHHHHHHHHHHHHHHHHH
VYSGLFSPTIFMNSGVFIRRKVFDIVGKFDPQTLVDDFDLALRLWKRKLKVKFVRGKRCK
HHHCCCCCCEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCEE
IQYAFRLQDLFKQHCRWYIGGIKKIFEQFSEGYYLGIIAIVAIGAFVLFPILMLVLAYLL
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KADYLLSTVMPLFWGILYSSSLFSYLFHDGHRPKEILINTIIGVPVMYAFFQIAILVSFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSFEKKSTWYKVKREKI
HHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MVSFVYGVIIGFVFAFIGYVKNHFTKKLLDVNYSDDVDYSVVAVIAAKNEESVIENTVRN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHHH
LLTNSPRSFRVIVVDDKSTDSTYKILSELSREFPNLTVVRNTGAPGKSEALNVALDLVKE
HHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHH
DIVLFLDADARVDWDFIKEYSKLFRSPEINVVFTDFESYNQSRTLAVILQDLYFSFIKAF
CEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECHHHCCCCHHHHHHHHHHHHHHHHHH
VYSGLFSPTIFMNSGVFIRRKVFDIVGKFDPQTLVDDFDLALRLWKRKLKVKFVRGKRCK
HHHCCCCCCEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCEE
IQYAFRLQDLFKQHCRWYIGGIKKIFEQFSEGYYLGIIAIVAIGAFVLFPILMLVLAYLL
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KADYLLSTVMPLFWGILYSSSLFSYLFHDGHRPKEILINTIIGVPVMYAFFQIAILVSFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSFEKKSTWYKVKREKI
HHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]