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The map label for this gene is ycsA [H]

Identifier: 239617381

GI number: 239617381

Start: 1046869

End: 1047936

Strand: Reverse

Name: ycsA [H]

Synonym: Kole_0991

Alternate gene names: 239617381

Gene position: 1047936-1046869 (Counterclockwise)

Preceding gene: 239617382

Following gene: 239617380

Centisome position: 45.52

GC content: 45.32

Gene sequence:

>1068_bases
ATGAGAAAGTACAAAATCGCGGTGATACCGGGTGACGGGATAGGCGTAGATGTTATTAACGAAGGGCTAAAAATCCTCGA
AACAGCTAAAGAACTCCACGGTGGCTTTGATTTTGAATACACCCATTTGCCCTGGGGCTGTGAATATTACAAAAAAACCG
GAAGAATGATGCCTGAAGACGGTTTGAGAATCCTGAAAGATCATGAGGCTATATATCTTGGTGCTGTCGGATTCCCAGGT
GTACCGGATCATGTGTCATTGTGGGGACTGCTTCTGCCTATAAGAAAGGAATTCGATGAATACATAAACCTGAGACCGAT
AAAGTTGTTGAAGGGCATACAGGGCCCCCTGAGAAAAGGCCCTGAGGATATAGATTTCGTTGTCGTCAGGGAAAACGTTG
AAGGGGAGTACTCCGGAGTTGGAGGTCTGGCGTATCAGGGAACTGCATCCGAAATCGCGTTGCAAACCAATGTATTTACG
CGTTTTGGAACGGAACGAGTTATGAGATATGCCTTCGAACTGGCAAGAAAGAGAAAGAAAAAACTTATATCCGCCACAAA
ATCTAACGCTTTGAATTACAGCATGGTCTTCTGGGACAGGGTTTTCAACGAAGTTGCCAAAGATTACCAAGACGTTCAAA
CACAGGTTATGCACGTTGATGCTTTGTCTGGATACTTCATTTTGCATCCTGAAAGGATAGATGTGGTCGTTGCATCAAAT
CTTTTCGGGGATATTCTAACGGATCTAGGATCCGCAATGCAGGGAAGCATCGGTATAGCACCCGGTGCCAACATCAATCC
AGAGAGAAAGTATCCTTCCATGTTTGAGCCCGTTCATGGTTCCGCACCAGATGTTGCTGGCAAGGGAATAGCAAATCCTA
TCGGAGCCATATGGGCCGCTTCCATGATGCTTGACTTTTTGGGAGTACCTGAAGCTGCCAAATTGATATTCGATGCTATG
GAAGCAGTTACAGAGGAAGGAAAATACCTGACCGTAGATCTGGGCGGAAATTCAACTACCCAGGAAGTAGGAGAGGCCAT
AAGAAATAAAATGAGAGAACTCGTGTGA

Upstream 100 bases:

>100_bases
TCGGAAGTGTTGATTATCTCAAGAAACGATTCCAACTTACTTCCAACGATATAGTTAAAAGCGTAAAGAAAGTACTTTCC
AGGAAGAAGAGGTGTATGTA

Downstream 100 bases:

>100_bases
ATTTCAGAGGGGGCGGATGGTGATGTTTCTACTCAAACCAGATGGAATTTTTCAATGGGGATATACGTCAATTACAGAGG
AAGGAGAAGCATCTGGAAAT

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: TDH; D-malate dehydrogenase [decarboxylating] [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MRKYKIAVIPGDGIGVDVINEGLKILETAKELHGGFDFEYTHLPWGCEYYKKTGRMMPEDGLRILKDHEAIYLGAVGFPG
VPDHVSLWGLLLPIRKEFDEYINLRPIKLLKGIQGPLRKGPEDIDFVVVRENVEGEYSGVGGLAYQGTASEIALQTNVFT
RFGTERVMRYAFELARKRKKKLISATKSNALNYSMVFWDRVFNEVAKDYQDVQTQVMHVDALSGYFILHPERIDVVVASN
LFGDILTDLGSAMQGSIGIAPGANINPERKYPSMFEPVHGSAPDVAGKGIANPIGAIWAASMMLDFLGVPEAAKLIFDAM
EAVTEEGKYLTVDLGGNSTTQEVGEAIRNKMRELV

Sequences:

>Translated_355_residues
MRKYKIAVIPGDGIGVDVINEGLKILETAKELHGGFDFEYTHLPWGCEYYKKTGRMMPEDGLRILKDHEAIYLGAVGFPG
VPDHVSLWGLLLPIRKEFDEYINLRPIKLLKGIQGPLRKGPEDIDFVVVRENVEGEYSGVGGLAYQGTASEIALQTNVFT
RFGTERVMRYAFELARKRKKKLISATKSNALNYSMVFWDRVFNEVAKDYQDVQTQVMHVDALSGYFILHPERIDVVVASN
LFGDILTDLGSAMQGSIGIAPGANINPERKYPSMFEPVHGSAPDVAGKGIANPIGAIWAASMMLDFLGVPEAAKLIFDAM
EAVTEEGKYLTVDLGGNSTTQEVGEAIRNKMRELV
>Mature_355_residues
MRKYKIAVIPGDGIGVDVINEGLKILETAKELHGGFDFEYTHLPWGCEYYKKTGRMMPEDGLRILKDHEAIYLGAVGFPG
VPDHVSLWGLLLPIRKEFDEYINLRPIKLLKGIQGPLRKGPEDIDFVVVRENVEGEYSGVGGLAYQGTASEIALQTNVFT
RFGTERVMRYAFELARKRKKKLISATKSNALNYSMVFWDRVFNEVAKDYQDVQTQVMHVDALSGYFILHPERIDVVVASN
LFGDILTDLGSAMQGSIGIAPGANINPERKYPSMFEPVHGSAPDVAGKGIANPIGAIWAASMMLDFLGVPEAAKLIFDAM
EAVTEEGKYLTVDLGGNSTTQEVGEAIRNKMRELV

Specific function: Unknown

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=352, Percent_Identity=32.6704545454545, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI4758582, Length=361, Percent_Identity=27.4238227146814, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI28178816, Length=347, Percent_Identity=26.2247838616715, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178821, Length=342, Percent_Identity=26.6081871345029, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI28178838, Length=329, Percent_Identity=27.0516717325228, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178819, Length=193, Percent_Identity=30.0518134715026, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=53.6516853932584, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI87081683, Length=364, Percent_Identity=36.5384615384615, Blast_Score=200, Evalue=9e-53,
Organism=Escherichia coli, GI1787381, Length=373, Percent_Identity=28.1501340482574, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71986051, Length=351, Percent_Identity=30.7692307692308, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17550882, Length=355, Percent_Identity=30.1408450704225, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=350, Percent_Identity=27.1428571428571, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=363, Percent_Identity=28.3746556473829, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322097, Length=364, Percent_Identity=35.4395604395604, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319830, Length=373, Percent_Identity=31.6353887399464, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6324291, Length=361, Percent_Identity=30.1939058171745, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324709, Length=358, Percent_Identity=31.8435754189944, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24643270, Length=352, Percent_Identity=31.25, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24643268, Length=352, Percent_Identity=31.25, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24661184, Length=350, Percent_Identity=30.8571428571429, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI161078635, Length=258, Percent_Identity=32.9457364341085, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078633, Length=258, Percent_Identity=32.9457364341085, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24650122, Length=258, Percent_Identity=32.9457364341085, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078639, Length=258, Percent_Identity=32.9457364341085, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078637, Length=258, Percent_Identity=32.9457364341085, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281362242, Length=350, Percent_Identity=27.1428571428571, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24648872, Length=350, Percent_Identity=27.1428571428571, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI20130355, Length=256, Percent_Identity=27.734375, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.93; =4.1.1.73; =1.1.1.83 [H]

Molecular weight: Translated: 39263; Mature: 39263

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKYKIAVIPGDGIGVDVINEGLKILETAKELHGGFDFEYTHLPWGCEYYKKTGRMMPED
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCC
GLRILKDHEAIYLGAVGFPGVPDHVSLWGLLLPIRKEFDEYINLRPIKLLKGIQGPLRKG
CHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHCC
PEDIDFVVVRENVEGEYSGVGGLAYQGTASEIALQTNVFTRFGTERVMRYAFELARKRKK
CCCCEEEEEEECCCCCCCCCCCEEECCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHH
KLISATKSNALNYSMVFWDRVFNEVAKDYQDVQTQVMHVDALSGYFILHPERIDVVVASN
HHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEHHH
LFGDILTDLGSAMQGSIGIAPGANINPERKYPSMFEPVHGSAPDVAGKGIANPIGAIWAA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHH
SMMLDFLGVPEAAKLIFDAMEAVTEEGKYLTVDLGGNSTTQEVGEAIRNKMRELV
HHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKYKIAVIPGDGIGVDVINEGLKILETAKELHGGFDFEYTHLPWGCEYYKKTGRMMPED
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCC
GLRILKDHEAIYLGAVGFPGVPDHVSLWGLLLPIRKEFDEYINLRPIKLLKGIQGPLRKG
CHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHCC
PEDIDFVVVRENVEGEYSGVGGLAYQGTASEIALQTNVFTRFGTERVMRYAFELARKRKK
CCCCEEEEEEECCCCCCCCCCCEEECCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHH
KLISATKSNALNYSMVFWDRVFNEVAKDYQDVQTQVMHVDALSGYFILHPERIDVVVASN
HHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEHHH
LFGDILTDLGSAMQGSIGIAPGANINPERKYPSMFEPVHGSAPDVAGKGIANPIGAIWAA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHH
SMMLDFLGVPEAAKLIFDAMEAVTEEGKYLTVDLGGNSTTQEVGEAIRNKMRELV
HHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]