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The map label for this gene is cofE [H]

Identifier: 239617310

GI number: 239617310

Start: 972789

End: 973760

Strand: Direct

Name: cofE [H]

Synonym: Kole_0919

Alternate gene names: 239617310

Gene position: 972789-973760 (Clockwise)

Preceding gene: 239617307

Following gene: 239617313

Centisome position: 42.26

GC content: 40.64

Gene sequence:

>972_bases
GTGTCAGAACAAACTGTAAAAGGAGAACTCAGGGTTATACCGATAAGTGTTTCGAAGGCTATCGTACCCGAGGAGGCTCG
AAGAACTACAGCTGGAGAATATCTTGTTAATGCCCTTAAAGAAAAAGGAATAGAGGTTCAGGATAAGGATGTTTTTGTAC
TCACTTCAAAATTAGTCTCGCTCTTCGATGGTAGAACAATCAATTTGAGTACAGTGACTCCCTCGAGAAAAGCGAAGATT
CTTGGCAAACTTTTCCATAAAGATCCCCGTGAGGTTGAACTGATAATGCGTGAAGGAAAGGTTCTGGCTGTTATACCTTT
CAAAAAAATTGCGAAAAACCCCGCTATATGGAAGCGAATGCTCCAGTTTTCGGCTAACCCCGAAGGTACACGCCAAGCGC
TCGATATATCAACATATGTTTTTGTTGTCAATAACCACGCAGCTTATCTCGATGACGCTGGTATAGATTTTTCCAATTCT
CCACCAGATTATGTAACGCTACTACCTAAGGACCCTTGTGCCAGCGCTAAAAGAATTCGAAAAGAAATCATGGATTTAAC
CGGTAAAGAAGTTGCTGTGATTATCACCGATACCGTTTCCGTGTTAGGAAGAATGGGATCGCAAGACATTGCCATAGGTT
ATTCTGGTATTGACCCAATTACAAGAAAAAGCGGTAAGGAAGATCTTTTTGGAGAGCCTCATCTTGGAGGAAACGACCTT
GTTATTGATTCTATGGCTGGAATTGCAGGACTTGTCATGGGACAGATGAACGAAAGTACTCCTGCGACACTTATCAGAGG
ATATGATTACGAGCCAGAAAGAGAAGATGAAGAACCTAAAGGTATGGAGTTAATAGCTTATCCGAAGAGTATGGCTTTTA
GATCAAGTGTTCTAACGATATTAACAACTGTCTGGTTTCGCATAGTCAATTTTTTTACCTTTCAAAAATGGCCAAAAGAT
GAAGAAAATTAA

Upstream 100 bases:

>100_bases
CCTCGCAAAAATTATACCCCCTTAGATTGAACATATCTTCAATATGTTCCGGTGATATAATAAAAAAAAGAAAAAGCGAG
ATTTGAAAGGAGGCACATAT

Downstream 100 bases:

>100_bases
AACGGCGGGGCTCAAAACCCGCCTTTTTAATAGCTTTCAACTTTGATTATCCGATATCCCTTTGCTGAAAGTTTCTCCTC
AAGATTCTCAACGATTTCAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSEQTVKGELRVIPISVSKAIVPEEARRTTAGEYLVNALKEKGIEVQDKDVFVLTSKLVSLFDGRTINLSTVTPSRKAKI
LGKLFHKDPREVELIMREGKVLAVIPFKKIAKNPAIWKRMLQFSANPEGTRQALDISTYVFVVNNHAAYLDDAGIDFSNS
PPDYVTLLPKDPCASAKRIRKEIMDLTGKEVAVIITDTVSVLGRMGSQDIAIGYSGIDPITRKSGKEDLFGEPHLGGNDL
VIDSMAGIAGLVMGQMNESTPATLIRGYDYEPEREDEEPKGMELIAYPKSMAFRSSVLTILTTVWFRIVNFFTFQKWPKD
EEN

Sequences:

>Translated_323_residues
MSEQTVKGELRVIPISVSKAIVPEEARRTTAGEYLVNALKEKGIEVQDKDVFVLTSKLVSLFDGRTINLSTVTPSRKAKI
LGKLFHKDPREVELIMREGKVLAVIPFKKIAKNPAIWKRMLQFSANPEGTRQALDISTYVFVVNNHAAYLDDAGIDFSNS
PPDYVTLLPKDPCASAKRIRKEIMDLTGKEVAVIITDTVSVLGRMGSQDIAIGYSGIDPITRKSGKEDLFGEPHLGGNDL
VIDSMAGIAGLVMGQMNESTPATLIRGYDYEPEREDEEPKGMELIAYPKSMAFRSSVLTILTTVWFRIVNFFTFQKWPKD
EEN
>Mature_322_residues
SEQTVKGELRVIPISVSKAIVPEEARRTTAGEYLVNALKEKGIEVQDKDVFVLTSKLVSLFDGRTINLSTVTPSRKAKIL
GKLFHKDPREVELIMREGKVLAVIPFKKIAKNPAIWKRMLQFSANPEGTRQALDISTYVFVVNNHAAYLDDAGIDFSNSP
PDYVTLLPKDPCASAKRIRKEIMDLTGKEVAVIITDTVSVLGRMGSQDIAIGYSGIDPITRKSGKEDLFGEPHLGGNDLV
IDSMAGIAGLVMGQMNESTPATLIRGYDYEPEREDEEPKGMELIAYPKSMAFRSSVLTILTTVWFRIVNFFTFQKWPKDE
EN

Specific function: Catalyzes the GTP-dependent successive addition of two L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8- hydroxy-5-deazariboflavin (F420-0) to form F420-0-glutamyl- glutamate (F420-2), with a gamma-linkage between the two glutamates. Cannot

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 35914; Mature: 35783

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQTVKGELRVIPISVSKAIVPEEARRTTAGEYLVNALKEKGIEVQDKDVFVLTSKLVS
CCCCCCCCCEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHH
LFDGRTINLSTVTPSRKAKILGKLFHKDPREVELIMREGKVLAVIPFKKIAKNPAIWKRM
HHCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECHHHHHCCHHHHHHH
LQFSANPEGTRQALDISTYVFVVNNHAAYLDDAGIDFSNSPPDYVTLLPKDPCASAKRIR
HHHCCCCCCHHHEEEEEEEEEEEECCEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHH
KEIMDLTGKEVAVIITDTVSVLGRMGSQDIAIGYSGIDPITRKSGKEDLFGEPHLGGNDL
HHHHHCCCCEEEEEEEHHHHHHHCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCCCCE
VIDSMAGIAGLVMGQMNESTPATLIRGYDYEPEREDEEPKGMELIAYPKSMAFRSSVLTI
EEHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
LTTVWFRIVNFFTFQKWPKDEEN
HHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SEQTVKGELRVIPISVSKAIVPEEARRTTAGEYLVNALKEKGIEVQDKDVFVLTSKLVS
CCCCCCCCEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHH
LFDGRTINLSTVTPSRKAKILGKLFHKDPREVELIMREGKVLAVIPFKKIAKNPAIWKRM
HHCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECHHHHHCCHHHHHHH
LQFSANPEGTRQALDISTYVFVVNNHAAYLDDAGIDFSNSPPDYVTLLPKDPCASAKRIR
HHHCCCCCCHHHEEEEEEEEEEEECCEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHH
KEIMDLTGKEVAVIITDTVSVLGRMGSQDIAIGYSGIDPITRKSGKEDLFGEPHLGGNDL
HHHHHCCCCEEEEEEEHHHHHHHCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCCCCE
VIDSMAGIAGLVMGQMNESTPATLIRGYDYEPEREDEEPKGMELIAYPKSMAFRSSVLTI
EEHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
LTTVWFRIVNFFTFQKWPKDEEN
HHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]