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The map label for this gene is ushA [H]

Identifier: 239617293

GI number: 239617293

Start: 953940

End: 955421

Strand: Direct

Name: ushA [H]

Synonym: Kole_0902

Alternate gene names: 239617293

Gene position: 953940-955421 (Clockwise)

Preceding gene: 239617292

Following gene: 239617294

Centisome position: 41.44

GC content: 44.26

Gene sequence:

>1482_bases
ATGAGAAAACTGCTTCTAGTATCGCTATTAGTACTCATTGCGGTATTTGTTCTTGCAGAAAAGATAACCATCCTGCACAT
CAACGACACACACGGGCATGCATGGACTTTTAGTGAATGGAAAAATCCCGATATAGGTGGTTTTGCTTTGATTGCAACCC
TGGTTGATCAATTCAGGAAAGAGAATCCCAATACGTTGTTTTTGCACGCTGGCGATCTCAATACCGGTGTACCTGAATCC
GACCTCATAGACGCCGCAGCGGATATCGTTGCATTGAACCTCATGGGACTGGATGCGATCGTCCTTGGAAACCATGAATT
CGACAACCCACCGGAAGTTCTGGCAAAACAGATAAAATGGGCTCACTTCCCATTCTTGAGTGCAAACATTTATAAAGATG
GAAAACCTGCTTTCACCCCTTACATAATTAAAGAAGTCGGTGGATTGAAAGTTGCGATCGTTGGTTTCACCACCGAGGAA
ACGGAAATCCTCGAAGCCCTTTATGCTCAAGGTTATGAATGGAAGAGTGTCATCGAAGTCGCCAAGGAATTGGTTCCTGA
ACTGAAAAAACAGGCAGACCTTGTTATTGCCCTAACACATCTTGGTACTGGCAAACCTATCGTAGGTGTTAACTCTATCG
AACTCGCTGAACAAGTTGATGGCATAGATGTCATTGTTGACGGTCACAGTCATTCTCTTCTCACCGAACCAATGGTTGTA
AATGAAACTCTCATCATTCAAGCTGGAGAATGGGGCAAATACCTCGGTAAGCTCGATCTCGAAGTAGAAAATGGCGGAAT
CGTTTTCGTCAGCTACGAATCCATCCCGATAAAGAAAGCCAATATAGAATCCAACAGAGCTGTTGATATCGTTCTTGATT
ATTTCAAGAAGCTTGGGGATGAAAAACTCGATACCGTTGTTGGTGAAACCAAGATTCTGCTTGAAGGAACAAGAGCAATA
ATCAGAAATCAGGATACAAATCTCGGTCACCTTGTAGCTGATGCCATGGTCTGGAAGACTGGTGCCGACCTCGCCATAAC
CAACGCAGGTGGAATCAGAGCCTCAATTCAGCCCGGACCGATAACATACCGCGATATTCTCAAGGTTCTTCCGTTTGGCA
ATACCCTTTACATCATAAAGATCACAGGGGAACAGTTGATGGAAGTACTCAAATACACCACAACGATTCCAGCTGGAAAA
GGTGCCAGACCACAAGTAGCAAATCTTAGCTACAAAATTGAAAACGGCGAGGTCAAGGATGTTATGATCAAAGGAGAGCC
AATAGATCTCAGTAAGACCTACACTCTCGTTACAAACAACTATATGGCTGCCGGTGGAGACGGTTACAAAATGCTCAAAG
GACTCGAAGGTTATGACACCGGATTCGTCCTTGCCGATGTCGTCATGGAATATATCTCTGAAATCAGCCCGATTACCGAG
TACGACGATGCTCCAAGGGTAATAAAAGTCGAAGCTGAATAA

Upstream 100 bases:

>100_bases
CACCTGAGCAAGGTGTCACATTCGAATTCATAGGCTCTATCGAACCCTTAGATACAGGGGAAGGAGAAAAAAACAAAAAA
GGAAGAAGGAGGTAGGGAAA

Downstream 100 bases:

>100_bases
GCCAATTGAAAATGTCGCTGATTTGACATTTTAAAACCTCGAAAATTTGAGGAGTAGTTTATAAATATTTCCTGTATTAT
AATAGGGGCGTAAGCCCCTA

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MRKLLLVSLLVLIAVFVLAEKITILHINDTHGHAWTFSEWKNPDIGGFALIATLVDQFRKENPNTLFLHAGDLNTGVPES
DLIDAAADIVALNLMGLDAIVLGNHEFDNPPEVLAKQIKWAHFPFLSANIYKDGKPAFTPYIIKEVGGLKVAIVGFTTEE
TEILEALYAQGYEWKSVIEVAKELVPELKKQADLVIALTHLGTGKPIVGVNSIELAEQVDGIDVIVDGHSHSLLTEPMVV
NETLIIQAGEWGKYLGKLDLEVENGGIVFVSYESIPIKKANIESNRAVDIVLDYFKKLGDEKLDTVVGETKILLEGTRAI
IRNQDTNLGHLVADAMVWKTGADLAITNAGGIRASIQPGPITYRDILKVLPFGNTLYIIKITGEQLMEVLKYTTTIPAGK
GARPQVANLSYKIENGEVKDVMIKGEPIDLSKTYTLVTNNYMAAGGDGYKMLKGLEGYDTGFVLADVVMEYISEISPITE
YDDAPRVIKVEAE

Sequences:

>Translated_493_residues
MRKLLLVSLLVLIAVFVLAEKITILHINDTHGHAWTFSEWKNPDIGGFALIATLVDQFRKENPNTLFLHAGDLNTGVPES
DLIDAAADIVALNLMGLDAIVLGNHEFDNPPEVLAKQIKWAHFPFLSANIYKDGKPAFTPYIIKEVGGLKVAIVGFTTEE
TEILEALYAQGYEWKSVIEVAKELVPELKKQADLVIALTHLGTGKPIVGVNSIELAEQVDGIDVIVDGHSHSLLTEPMVV
NETLIIQAGEWGKYLGKLDLEVENGGIVFVSYESIPIKKANIESNRAVDIVLDYFKKLGDEKLDTVVGETKILLEGTRAI
IRNQDTNLGHLVADAMVWKTGADLAITNAGGIRASIQPGPITYRDILKVLPFGNTLYIIKITGEQLMEVLKYTTTIPAGK
GARPQVANLSYKIENGEVKDVMIKGEPIDLSKTYTLVTNNYMAAGGDGYKMLKGLEGYDTGFVLADVVMEYISEISPITE
YDDAPRVIKVEAE
>Mature_493_residues
MRKLLLVSLLVLIAVFVLAEKITILHINDTHGHAWTFSEWKNPDIGGFALIATLVDQFRKENPNTLFLHAGDLNTGVPES
DLIDAAADIVALNLMGLDAIVLGNHEFDNPPEVLAKQIKWAHFPFLSANIYKDGKPAFTPYIIKEVGGLKVAIVGFTTEE
TEILEALYAQGYEWKSVIEVAKELVPELKKQADLVIALTHLGTGKPIVGVNSIELAEQVDGIDVIVDGHSHSLLTEPMVV
NETLIIQAGEWGKYLGKLDLEVENGGIVFVSYESIPIKKANIESNRAVDIVLDYFKKLGDEKLDTVVGETKILLEGTRAI
IRNQDTNLGHLVADAMVWKTGADLAITNAGGIRASIQPGPITYRDILKVLPFGNTLYIIKITGEQLMEVLKYTTTIPAGK
GARPQVANLSYKIENGEVKDVMIKGEPIDLSKTYTLVTNNYMAAGGDGYKMLKGLEGYDTGFVLADVVMEYISEISPITE
YDDAPRVIKVEAE

Specific function: Degradation of extracellular 5'-nucleotides for nutritional needs [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=535, Percent_Identity=29.7196261682243, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1786687, Length=542, Percent_Identity=37.4538745387454, Blast_Score=324, Evalue=8e-90,
Organism=Escherichia coli, GI1790658, Length=562, Percent_Identity=23.6654804270463, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI28573524, Length=500, Percent_Identity=28.2, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI19922446, Length=503, Percent_Identity=29.6222664015905, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24654424, Length=503, Percent_Identity=29.6222664015905, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI19921980, Length=451, Percent_Identity=28.8248337028825, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24652512, Length=451, Percent_Identity=28.8248337028825, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI161076508, Length=451, Percent_Identity=28.8248337028825, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI19922444, Length=534, Percent_Identity=27.7153558052434, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24641187, Length=538, Percent_Identity=26.9516728624535, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI221329836, Length=528, Percent_Identity=25.1893939393939, Blast_Score=115, Evalue=8e-26,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 53966; Mature: 53966

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLLLVSLLVLIAVFVLAEKITILHINDTHGHAWTFSEWKNPDIGGFALIATLVDQFRK
CHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHH
ENPNTLFLHAGDLNTGVPESDLIDAAADIVALNLMGLDAIVLGNHEFDNPPEVLAKQIKW
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHEEEEECCEEEEECCCCCCCCHHHHHHHHHH
AHFPFLSANIYKDGKPAFTPYIIKEVGGLKVAIVGFTTEETEILEALYAQGYEWKSVIEV
HCCCEEECEEECCCCCCCCHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHH
AKELVPELKKQADLVIALTHLGTGKPIVGVNSIELAEQVDGIDVIVDGHSHSLLTEPMVV
HHHHHHHHHHCCCEEEEEEECCCCCCEECCCCHHHHHHCCCEEEEECCCCCCCCCCCEEE
NETLIIQAGEWGKYLGKLDLEVENGGIVFVSYESIPIKKANIESNRAVDIVLDYFKKLGD
CCEEEEECCCHHHCCCEEEEEEECCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHCC
EKLDTVVGETKILLEGTRAIIRNQDTNLGHLVADAMVWKTGADLAITNAGGIRASIQPGP
HHHHHHHCCEEEEEECCHHHHCCCCCCHHHHHHHHHHEECCCCEEEECCCCEEEECCCCC
ITYRDILKVLPFGNTLYIIKITGEQLMEVLKYTTTIPAGKGARPQVANLSYKIENGEVKD
CCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEECEEEEECCCCEEE
VMIKGEPIDLSKTYTLVTNNYMAAGGDGYKMLKGLEGYDTGFVLADVVMEYISEISPITE
EEEECCCCCCCCEEEEEECCEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
YDDAPRVIKVEAE
CCCCCCEEEEECC
>Mature Secondary Structure
MRKLLLVSLLVLIAVFVLAEKITILHINDTHGHAWTFSEWKNPDIGGFALIATLVDQFRK
CHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHH
ENPNTLFLHAGDLNTGVPESDLIDAAADIVALNLMGLDAIVLGNHEFDNPPEVLAKQIKW
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHEEEEECCEEEEECCCCCCCCHHHHHHHHHH
AHFPFLSANIYKDGKPAFTPYIIKEVGGLKVAIVGFTTEETEILEALYAQGYEWKSVIEV
HCCCEEECEEECCCCCCCCHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHH
AKELVPELKKQADLVIALTHLGTGKPIVGVNSIELAEQVDGIDVIVDGHSHSLLTEPMVV
HHHHHHHHHHCCCEEEEEEECCCCCCEECCCCHHHHHHCCCEEEEECCCCCCCCCCCEEE
NETLIIQAGEWGKYLGKLDLEVENGGIVFVSYESIPIKKANIESNRAVDIVLDYFKKLGD
CCEEEEECCCHHHCCCEEEEEEECCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHCC
EKLDTVVGETKILLEGTRAIIRNQDTNLGHLVADAMVWKTGADLAITNAGGIRASIQPGP
HHHHHHHCCEEEEEECCHHHHCCCCCCHHHHHHHHHHEECCCCEEEECCCCEEEECCCCC
ITYRDILKVLPFGNTLYIIKITGEQLMEVLKYTTTIPAGKGARPQVANLSYKIENGEVKD
CCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEECEEEEECCCCEEE
VMIKGEPIDLSKTYTLVTNNYMAAGGDGYKMLKGLEGYDTGFVLADVVMEYISEISPITE
EEEECCCCCCCCEEEEEECCEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC
YDDAPRVIKVEAE
CCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]