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The map label for this gene is rlmI [H]

Identifier: 239617288

GI number: 239617288

Start: 949172

End: 950353

Strand: Direct

Name: rlmI [H]

Synonym: Kole_0897

Alternate gene names: 239617288

Gene position: 949172-950353 (Clockwise)

Preceding gene: 239617287

Following gene: 239617289

Centisome position: 41.23

GC content: 39.34

Gene sequence:

>1182_bases
ATGGTCATAGCTGTTTTAAAAAAAGGAAAGGAAAAGAAGCTCCTCAACGGTTACCCGTGGGTTTTTGAAGATGAAATCAG
TGAAGTTCAGGGTGAGCTAATCCTTGGCTCCCCGGTGAATGTTTTTTCGAATGATTGGGCCTTCGTGGGAAAAGGCTTAT
ACAATCCCTTTTCGAACAGAAAGATAATGTTTCTGACCACTGAAGATGTAGAGTTAGACGAAAATTTTTTCAGAGAAAGA
TTATATAAAGCCCTGGAATGGAGAACACGTTTTTTTGATAAGCCCTATTACAGGTTATTTCACGGTGAAGCCGACGGAAT
TCCCGGGTTCATTGCCGACAGGTATGGTGAAGTTATAGTTGTACAGTTCAGAAATTCCATTGTAGAACTATTGAAAAAAC
AGCTGGTAAATTCTATCGTAGAGCTTCTTCTTCCTGCTGCGGTTTATGAGAGAAGCGATTTTGAAATGGGCACCGGTGAA
GCTTTGCAAAGGGAAACGGGACTACTTTTTGGTGAGATAAAAACTCCAATTGTTGAGGTCGAAGAGAATGGATTGAAGTA
TATCGTGAACTTTACAAACGGGCAAAAAACCGGGTTTTTCTTCGACCAAAGAGATTCGAGAAAAATGGCACGGAAAATTG
TAAAAAGATTCGCTTTCAAGAGAGCTCTCGATCTTTTTTCGTTTACCGGTGGTTTTGGAATAAATATGGCCAAAGCGGGT
GCTGAAGTTGTTTGCGTCGATAAATCCGGTGAAGATCTAGAAGTAGCTGAACTCAACGCAGCGTTGAATGATGTAAAAGA
ACGCATGAGTTTTGTTCAGATGGACGTCTTCAAATATCTGAAATCTTTGGAAAAGAAGGAACAATTTGATATAGTTGTTT
TAGATCCTCCATCGTTGATAAAAAGAAAGAAGGAACTTCGTAGAGGAATAGAACTTTTAAAAGAATTAGTCTCTCTCTCC
GTTCCGGTAGTTAAAAGCCACGGAGTAATTTGCCTTTGCAGTTGCGCTTATAATCTTGGGCTAGAACATCTTATTGAAGC
ATTAAGAAAGGTATCTTTGGAATTAGACGTTCAGTATAAAAATATTGGGATAACCTACCAGTCTTTAGATCATCCCTGGG
TTTTGCAAATCCCGGAATCGCTTTATTTAAAGTGTGTGTGGACAGTTGTTGAAAAGAGGTGA

Upstream 100 bases:

>100_bases
ATGCTTCCGACAGTCCCGAGCATGCCAAACACGAAATGTCGATTTTCTTCGATACCAGTGAAATCGTGGATTATAAGCTC
TTACTAGAGGAACAATTCTG

Downstream 100 bases:

>100_bases
TAGAGGTGCCAGCTATATGGACCCACCTCATTCACGGTGACAGGGTTTTAAGAGAATTGAATATCGGTTTTGAAGAGGAT
TCCCTTTTCCTGTTCCATCT

Product: methyltransferase small

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MVIAVLKKGKEKKLLNGYPWVFEDEISEVQGELILGSPVNVFSNDWAFVGKGLYNPFSNRKIMFLTTEDVELDENFFRER
LYKALEWRTRFFDKPYYRLFHGEADGIPGFIADRYGEVIVVQFRNSIVELLKKQLVNSIVELLLPAAVYERSDFEMGTGE
ALQRETGLLFGEIKTPIVEVEENGLKYIVNFTNGQKTGFFFDQRDSRKMARKIVKRFAFKRALDLFSFTGGFGINMAKAG
AEVVCVDKSGEDLEVAELNAALNDVKERMSFVQMDVFKYLKSLEKKEQFDIVVLDPPSLIKRKKELRRGIELLKELVSLS
VPVVKSHGVICLCSCAYNLGLEHLIEALRKVSLELDVQYKNIGITYQSLDHPWVLQIPESLYLKCVWTVVEKR

Sequences:

>Translated_393_residues
MVIAVLKKGKEKKLLNGYPWVFEDEISEVQGELILGSPVNVFSNDWAFVGKGLYNPFSNRKIMFLTTEDVELDENFFRER
LYKALEWRTRFFDKPYYRLFHGEADGIPGFIADRYGEVIVVQFRNSIVELLKKQLVNSIVELLLPAAVYERSDFEMGTGE
ALQRETGLLFGEIKTPIVEVEENGLKYIVNFTNGQKTGFFFDQRDSRKMARKIVKRFAFKRALDLFSFTGGFGINMAKAG
AEVVCVDKSGEDLEVAELNAALNDVKERMSFVQMDVFKYLKSLEKKEQFDIVVLDPPSLIKRKKELRRGIELLKELVSLS
VPVVKSHGVICLCSCAYNLGLEHLIEALRKVSLELDVQYKNIGITYQSLDHPWVLQIPESLYLKCVWTVVEKR
>Mature_393_residues
MVIAVLKKGKEKKLLNGYPWVFEDEISEVQGELILGSPVNVFSNDWAFVGKGLYNPFSNRKIMFLTTEDVELDENFFRER
LYKALEWRTRFFDKPYYRLFHGEADGIPGFIADRYGEVIVVQFRNSIVELLKKQLVNSIVELLLPAAVYERSDFEMGTGE
ALQRETGLLFGEIKTPIVEVEENGLKYIVNFTNGQKTGFFFDQRDSRKMARKIVKRFAFKRALDLFSFTGGFGINMAKAG
AEVVCVDKSGEDLEVAELNAALNDVKERMSFVQMDVFKYLKSLEKKEQFDIVVLDPPSLIKRKKELRRGIELLKELVSLS
VPVVKSHGVICLCSCAYNLGLEHLIEALRKVSLELDVQYKNIGITYQSLDHPWVLQIPESLYLKCVWTVVEKR

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=389, Percent_Identity=33.6760925449871, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1787181, Length=133, Percent_Identity=35.3383458646617, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 45131; Mature: 45131

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIAVLKKGKEKKLLNGYPWVFEDEISEVQGELILGSPVNVFSNDWAFVGKGLYNPFSNR
CEEEEECCCCCCCCCCCCCCEECCCHHHCCCCEEECCCCCCCCCCHHHHCCCCCCCCCCC
KIMFLTTEDVELDENFFRERLYKALEWRTRFFDKPYYRLFHGEADGIPGFIADRYGEVIV
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCCCCHHHHCCCCEEE
VQFRNSIVELLKKQLVNSIVELLLPAAVYERSDFEMGTGEALQRETGLLFGEIKTPIVEV
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCEEECCCCCEEEE
EENGLKYIVNFTNGQKTGFFFDQRDSRKMARKIVKRFAFKRALDLFSFTGGFGINMAKAG
ECCCEEEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCC
AEVVCVDKSGEDLEVAELNAALNDVKERMSFVQMDVFKYLKSLEKKEQFDIVVLDPPSLI
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHH
KRKKELRRGIELLKELVSLSVPVVKSHGVICLCSCAYNLGLEHLIEALRKVSLELDVQYK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHCCHHHHHHHHHHCCEEEEEEEE
NIGITYQSLDHPWVLQIPESLYLKCVWTVVEKR
EEEEEEECCCCCEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVIAVLKKGKEKKLLNGYPWVFEDEISEVQGELILGSPVNVFSNDWAFVGKGLYNPFSNR
CEEEEECCCCCCCCCCCCCCEECCCHHHCCCCEEECCCCCCCCCCHHHHCCCCCCCCCCC
KIMFLTTEDVELDENFFRERLYKALEWRTRFFDKPYYRLFHGEADGIPGFIADRYGEVIV
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCCCCHHHHCCCCEEE
VQFRNSIVELLKKQLVNSIVELLLPAAVYERSDFEMGTGEALQRETGLLFGEIKTPIVEV
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCEEECCCCCEEEE
EENGLKYIVNFTNGQKTGFFFDQRDSRKMARKIVKRFAFKRALDLFSFTGGFGINMAKAG
ECCCEEEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCC
AEVVCVDKSGEDLEVAELNAALNDVKERMSFVQMDVFKYLKSLEKKEQFDIVVLDPPSLI
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHH
KRKKELRRGIELLKELVSLSVPVVKSHGVICLCSCAYNLGLEHLIEALRKVSLELDVQYK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHCCHHHHHHHHHHCCEEEEEEEE
NIGITYQSLDHPWVLQIPESLYLKCVWTVVEKR
EEEEEEECCCCCEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA