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The map label for this gene is yhfE [H]

Identifier: 239616989

GI number: 239616989

Start: 635516

End: 636550

Strand: Direct

Name: yhfE [H]

Synonym: Kole_0588

Alternate gene names: 239616989

Gene position: 635516-636550 (Clockwise)

Preceding gene: 239616988

Following gene: 239616990

Centisome position: 27.61

GC content: 42.42

Gene sequence:

>1035_bases
GTGGATTACAAAGAAAAATACGTTGTCAAGAAACTCGTGGATCTTGTCAAAATTCCCAGCCCTTCGGGTTTCACTCATAG
GGCCATTGAATATGTTCGCAAGGAACTCGAAACACTTGGTTTCGAACCTAAACTAACGAAAAAAGGCGCATGTTACGTGT
GCCTCGGCGGTGAAGGTAATCCAGTCGCTTTCACAGCACATGTCGATACTCTCGGTGCCATGGTTAAATCCTTGAAGTCA
AATGGGAGACTGGAAATCACTCCCATAGGCGGCTATATGATGAACAGTGTTGAGGGTGAAAACTGTGTGGTTCATACAAA
AGATGGAAGAGAGTACACGGGCACGATTCAAACTATCGCACCATCTGTTCATGTGTTTGAGAACGCAAGAACTCTAGAAA
GAAAACCTATAAATATGGAAGTACGGATCGATGAAAAAGTGAAAACCAGAGATGACCTCGAAAAACTCGGTATCGAACCG
GGCGATTTCATTTCTTTTGACCCGAGGATAGTCGTTACCGAGAAAGGGTTCATAAAGAGCCGCCATCTGGATGATAAAGC
AGGCGTAGCTATACTATTGACAGTAGCAAAAGACTTAAGCGAGAAAAAATTCTCGCTCAACAGAAAAGTCTACCTTTTCT
TTTCAAACTATGAAGAGGTTGGACACGGCGCTTCAAGTGGTATTCCCCAAGATGTTGAAGAACTGATATCCGTTGATATG
GGAGCTGTTGGTGGCACCCTTTCTACCAATGAATTCGTGGTTTCAATATGTGCTAAAGATTCCGGTGGACCCTATGACGC
TCGATTGGTAAGGAATCTAGTTGAAACTGCTAAAAAATCTGATGTAAATTACAGCGTAGATATCTATCCCTTCTACGGCT
CTGATGCCGATGCTTCTCTCAGAGCAGGTTACGACATTGTTCACGGCCTCTTAGGACCAGGTGTTGAAGCTTCACATGGA
TATGAGAGAACTCACTACGATGGTTTAAAGAATACGATTAAATTGATTGAAAAATATCTCAAAAAAATAGTCTAA

Upstream 100 bases:

>100_bases
TGAAACAATTAGAAACACTTATGTCAAAAGGCCTCACAGAGGATGAAGCCACGGAACTGATTGTTAGAGGAATGCTCAAA
TAGATTTAGGGGGGACTAAA

Downstream 100 bases:

>100_bases
AATACGTTTAACCAACAACACCGTGCTTTTTTGGTTGAAAGACCGTGATTTTTTCTTTTGGAAAATTGAGTTATCAGCGT
ATAATTATATTGAAGCATTA

Product: peptidase M42 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MDYKEKYVVKKLVDLVKIPSPSGFTHRAIEYVRKELETLGFEPKLTKKGACYVCLGGEGNPVAFTAHVDTLGAMVKSLKS
NGRLEITPIGGYMMNSVEGENCVVHTKDGREYTGTIQTIAPSVHVFENARTLERKPINMEVRIDEKVKTRDDLEKLGIEP
GDFISFDPRIVVTEKGFIKSRHLDDKAGVAILLTVAKDLSEKKFSLNRKVYLFFSNYEEVGHGASSGIPQDVEELISVDM
GAVGGTLSTNEFVVSICAKDSGGPYDARLVRNLVETAKKSDVNYSVDIYPFYGSDADASLRAGYDIVHGLLGPGVEASHG
YERTHYDGLKNTIKLIEKYLKKIV

Sequences:

>Translated_344_residues
MDYKEKYVVKKLVDLVKIPSPSGFTHRAIEYVRKELETLGFEPKLTKKGACYVCLGGEGNPVAFTAHVDTLGAMVKSLKS
NGRLEITPIGGYMMNSVEGENCVVHTKDGREYTGTIQTIAPSVHVFENARTLERKPINMEVRIDEKVKTRDDLEKLGIEP
GDFISFDPRIVVTEKGFIKSRHLDDKAGVAILLTVAKDLSEKKFSLNRKVYLFFSNYEEVGHGASSGIPQDVEELISVDM
GAVGGTLSTNEFVVSICAKDSGGPYDARLVRNLVETAKKSDVNYSVDIYPFYGSDADASLRAGYDIVHGLLGPGVEASHG
YERTHYDGLKNTIKLIEKYLKKIV
>Mature_344_residues
MDYKEKYVVKKLVDLVKIPSPSGFTHRAIEYVRKELETLGFEPKLTKKGACYVCLGGEGNPVAFTAHVDTLGAMVKSLKS
NGRLEITPIGGYMMNSVEGENCVVHTKDGREYTGTIQTIAPSVHVFENARTLERKPINMEVRIDEKVKTRDDLEKLGIEP
GDFISFDPRIVVTEKGFIKSRHLDDKAGVAILLTVAKDLSEKKFSLNRKVYLFFSNYEEVGHGASSGIPQDVEELISVDM
GAVGGTLSTNEFVVSICAKDSGGPYDARLVRNLVETAKKSDVNYSVDIYPFYGSDADASLRAGYDIVHGLLGPGVEASHG
YERTHYDGLKNTIKLIEKYLKKIV

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=167, Percent_Identity=28.1437125748503, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788727, Length=303, Percent_Identity=21.7821782178218, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: NA

Molecular weight: Translated: 37980; Mature: 37980

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYKEKYVVKKLVDLVKIPSPSGFTHRAIEYVRKELETLGFEPKLTKKGACYVCLGGEGN
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCC
PVAFTAHVDTLGAMVKSLKSNGRLEITPIGGYMMNSVEGENCVVHTKDGREYTGTIQTIA
EEEEEEEHHHHHHHHHHHHCCCCEEEEECCCHHCCCCCCCCEEEECCCCCEEECCHHHHC
PSVHVFENARTLERKPINMEVRIDEKVKTRDDLEKLGIEPGDFISFDPRIVVTEKGFIKS
CCEEEECCCHHHHCCCCEEEEEECCHHCCHHHHHHHCCCCCCEEEECCEEEEECCCCHHH
RHLDDKAGVAILLTVAKDLSEKKFSLNRKVYLFFSNYEEVGHGASSGIPQDVEELISVDM
CCCCCCCCEEEEEEEHHHHHHHHHCCCCEEEEEEECHHHHCCCCCCCCHHHHHHHHHHHC
GAVGGTLSTNEFVVSICAKDSGGPYDARLVRNLVETAKKSDVNYSVDIYPFYGSDADASL
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHH
RAGYDIVHGLLGPGVEASHGYERTHYDGLKNTIKLIEKYLKKIV
HCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDYKEKYVVKKLVDLVKIPSPSGFTHRAIEYVRKELETLGFEPKLTKKGACYVCLGGEGN
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCC
PVAFTAHVDTLGAMVKSLKSNGRLEITPIGGYMMNSVEGENCVVHTKDGREYTGTIQTIA
EEEEEEEHHHHHHHHHHHHCCCCEEEEECCCHHCCCCCCCCEEEECCCCCEEECCHHHHC
PSVHVFENARTLERKPINMEVRIDEKVKTRDDLEKLGIEPGDFISFDPRIVVTEKGFIKS
CCEEEECCCHHHHCCCCEEEEEECCHHCCHHHHHHHCCCCCCEEEECCEEEEECCCCHHH
RHLDDKAGVAILLTVAKDLSEKKFSLNRKVYLFFSNYEEVGHGASSGIPQDVEELISVDM
CCCCCCCCEEEEEEEHHHHHHHHHCCCCEEEEEEECHHHHCCCCCCCCHHHHHHHHHHHC
GAVGGTLSTNEFVVSICAKDSGGPYDARLVRNLVETAKKSDVNYSVDIYPFYGSDADASL
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHH
RAGYDIVHGLLGPGVEASHGYERTHYDGLKNTIKLIEKYLKKIV
HCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]