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The map label for this gene is pmbA [H]

Identifier: 239616956

GI number: 239616956

Start: 605263

End: 606633

Strand: Direct

Name: pmbA [H]

Synonym: Kole_0554

Alternate gene names: 239616956

Gene position: 605263-606633 (Clockwise)

Preceding gene: 239616955

Following gene: 239616980

Centisome position: 26.29

GC content: 40.63

Gene sequence:

>1371_bases
ATGACGTTCGAGAATTTTAAGAAAATGGTTTTTGAGTACGCAGCCGCTAAGAAAACCAATGATTTTGAAATCTACATAAA
GAAAACACACGAATTTGATGTCAGTATTCAGAAGGGTGAAATTGAGAAATACAAAGATGCTACAACATTGGGAGTGAGTT
TCAAAGTTCTCAAAGATGGAAAGGTGGGTTATTCTTTTTCCGAGGTGCTGGACGAGCAAGCGGTGAAACTGCTTGTTGAT
GAAGCACTGGAAAATGTAACAGTAATAGAGTCTGAGGAGTCCGACTTTCTTCACGATGGTTCAGGAGAGTACACTGACTT
TCCCACCCTTTATCTCGGAGAATTTGATAGACTGACAACACGCGAAAAAATCAAAATAGCGATGGAACTTGAAAAAACGA
TTATCAATTCGCACCAGGAGATCTTTATGCTTCCCTATAATGCATTCAACCACAGGACATCAGAGACCTACATAGCCAAT
TCCTCTGGAATGGAAATGTATTCCAGGTCAGATGGTGGATATGCTTACGTTATGGCATTGGGTAAAAAGAACGGGTCGCC
AAAGAGTGCTTTTTACTTCACGGCAGGAAAAAGTCTACAAGATCTTGAGCTTTCAAATATCCCCATAAGAATTTCCCGTG
ATATAGAACTGCTTCTCACTGCCAAACCTGTAAAGAGCGGTAAATACGCCGTTATTTTTAGAAACGATGCCTTTGGTTCG
CTCCTGGCAGCTTTTGCTCAGATGTTCTCAGCTAAATCAGTGCAAAAAGGTCTTTCTCTCTTGAAAGATCAGCTTGGGAA
AAAAATTGCTTCCGAAAAATTCACCTTGATTGACGACTCATTTTTGAAGATGAGCCCTGTTAGCAGAACCTTTGACGATC
AAGGCGTACCAACAAGGAAAAAAGCAATAATCGAAAATGGAGAATTGAAAACCTATCTGTACGATTTAAAAACTGCTAGA
ATCGACAATCGTGAGTCAACGGGTAACGCTTTGAAGATAGGAAGCTACAAAGATCCTACAAGTATTTCTTATTTCAATCT
TGTACTTGGAAATAGTGGAATCCCCTTCGAAGATATGGTAAAAGCTATCGAAAGAGGTCTGGTAATCATTGAACTTGATG
GATTGCATTCAGGAACCAATCCGATTTCTGGCGATTTTTCCCTTGGTGCCAGAGGCTTTTTGATAGAAAACGGAAGGATT
GTTGGAGGCGTTGAAGGGATAACAGTATCCGGTAACATCCTCGGCGTTTTGAAGAATATTGTCGAAACAGGTAACGATGC
AAAGTTTTCCTTGCCAGGTATAACAGGATTCTCCTCATTTGCATTCCTTCCCTCAGTTTTAGTAGAGGAGATCGATGTAG
CCGGGCTCTAA

Upstream 100 bases:

>100_bases
GCGGTCAGGGAATGTGTGGTTCAGCATCTGGTTTAATACCGGCTGAAGTAGGGCAACCAACCATTAGAGTCTCTGAATTG
ACCGTTGGGGGGCGAAATAG

Downstream 100 bases:

>100_bases
CAAGTTTTGGGGTATCTCATAGATTGAACTTGCTATAATTCTTGCTGTAGCTCCCCAACATTGTGATTCGGGGAGCTATT
TAGTTCTACTTAAAAACAAC

Product: peptidase U62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTFENFKKMVFEYAAAKKTNDFEIYIKKTHEFDVSIQKGEIEKYKDATTLGVSFKVLKDGKVGYSFSEVLDEQAVKLLVD
EALENVTVIESEESDFLHDGSGEYTDFPTLYLGEFDRLTTREKIKIAMELEKTIINSHQEIFMLPYNAFNHRTSETYIAN
SSGMEMYSRSDGGYAYVMALGKKNGSPKSAFYFTAGKSLQDLELSNIPIRISRDIELLLTAKPVKSGKYAVIFRNDAFGS
LLAAFAQMFSAKSVQKGLSLLKDQLGKKIASEKFTLIDDSFLKMSPVSRTFDDQGVPTRKKAIIENGELKTYLYDLKTAR
IDNRESTGNALKIGSYKDPTSISYFNLVLGNSGIPFEDMVKAIERGLVIIELDGLHSGTNPISGDFSLGARGFLIENGRI
VGGVEGITVSGNILGVLKNIVETGNDAKFSLPGITGFSSFAFLPSVLVEEIDVAGL

Sequences:

>Translated_456_residues
MTFENFKKMVFEYAAAKKTNDFEIYIKKTHEFDVSIQKGEIEKYKDATTLGVSFKVLKDGKVGYSFSEVLDEQAVKLLVD
EALENVTVIESEESDFLHDGSGEYTDFPTLYLGEFDRLTTREKIKIAMELEKTIINSHQEIFMLPYNAFNHRTSETYIAN
SSGMEMYSRSDGGYAYVMALGKKNGSPKSAFYFTAGKSLQDLELSNIPIRISRDIELLLTAKPVKSGKYAVIFRNDAFGS
LLAAFAQMFSAKSVQKGLSLLKDQLGKKIASEKFTLIDDSFLKMSPVSRTFDDQGVPTRKKAIIENGELKTYLYDLKTAR
IDNRESTGNALKIGSYKDPTSISYFNLVLGNSGIPFEDMVKAIERGLVIIELDGLHSGTNPISGDFSLGARGFLIENGRI
VGGVEGITVSGNILGVLKNIVETGNDAKFSLPGITGFSSFAFLPSVLVEEIDVAGL
>Mature_455_residues
TFENFKKMVFEYAAAKKTNDFEIYIKKTHEFDVSIQKGEIEKYKDATTLGVSFKVLKDGKVGYSFSEVLDEQAVKLLVDE
ALENVTVIESEESDFLHDGSGEYTDFPTLYLGEFDRLTTREKIKIAMELEKTIINSHQEIFMLPYNAFNHRTSETYIANS
SGMEMYSRSDGGYAYVMALGKKNGSPKSAFYFTAGKSLQDLELSNIPIRISRDIELLLTAKPVKSGKYAVIFRNDAFGSL
LAAFAQMFSAKSVQKGLSLLKDQLGKKIASEKFTLIDDSFLKMSPVSRTFDDQGVPTRKKAIIENGELKTYLYDLKTARI
DNRESTGNALKIGSYKDPTSISYFNLVLGNSGIPFEDMVKAIERGLVIIELDGLHSGTNPISGDFSLGARGFLIENGRIV
GGVEGITVSGNILGVLKNIVETGNDAKFSLPGITGFSSFAFLPSVLVEEIDVAGL

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=443, Percent_Identity=27.0880361173815, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI1789640, Length=213, Percent_Identity=27.6995305164319, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 50357; Mature: 50226

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFENFKKMVFEYAAAKKTNDFEIYIKKTHEFDVSIQKGEIEKYKDATTLGVSFKVLKDG
CCHHHHHHHHHHHHHHCCCCCEEEEEEEECEEEEEEECCCCHHHCCCEEECEEEEEEECC
KVGYSFSEVLDEQAVKLLVDEALENVTVIESEESDFLHDGSGEYTDFPTLYLGEFDRLTT
CCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEECCCCCCCCCCEEEECCCHHCCC
REKIKIAMELEKTIINSHQEIFMLPYNAFNHRTSETYIANSSGMEMYSRSDGGYAYVMAL
HHHEEEHHHHHHHHHCCCCEEEEECCHHHCCCCCCEEEECCCCCEEEECCCCCEEEEEEE
GKKNGSPKSAFYFTAGKSLQDLELSNIPIRISRDIELLLTAKPVKSGKYAVIFRNDAFGS
ECCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCCEEEEEECCCHHH
LLAAFAQMFSAKSVQKGLSLLKDQLGKKIASEKFTLIDDSFLKMSPVSRTFDDQGVPTRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCHH
KAIIENGELKTYLYDLKTARIDNRESTGNALKIGSYKDPTSISYFNLVLGNSGIPFEDMV
HHHEECCCEEEEEEEEHHHCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCHHHHH
KAIERGLVIIELDGLHSGTNPISGDFSLGARGFLIENGRIVGGVEGITVSGNILGVLKNI
HHHHCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCEEEECCCEEEECCHHHHHHHHH
VETGNDAKFSLPGITGFSSFAFLPSVLVEEIDVAGL
HHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TFENFKKMVFEYAAAKKTNDFEIYIKKTHEFDVSIQKGEIEKYKDATTLGVSFKVLKDG
CHHHHHHHHHHHHHHCCCCCEEEEEEEECEEEEEEECCCCHHHCCCEEECEEEEEEECC
KVGYSFSEVLDEQAVKLLVDEALENVTVIESEESDFLHDGSGEYTDFPTLYLGEFDRLTT
CCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEECCCCCCCCCCEEEECCCHHCCC
REKIKIAMELEKTIINSHQEIFMLPYNAFNHRTSETYIANSSGMEMYSRSDGGYAYVMAL
HHHEEEHHHHHHHHHCCCCEEEEECCHHHCCCCCCEEEECCCCCEEEECCCCCEEEEEEE
GKKNGSPKSAFYFTAGKSLQDLELSNIPIRISRDIELLLTAKPVKSGKYAVIFRNDAFGS
ECCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCCEEEEEECCCHHH
LLAAFAQMFSAKSVQKGLSLLKDQLGKKIASEKFTLIDDSFLKMSPVSRTFDDQGVPTRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCHH
KAIIENGELKTYLYDLKTARIDNRESTGNALKIGSYKDPTSISYFNLVLGNSGIPFEDMV
HHHEECCCEEEEEEEEHHHCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCHHHHH
KAIERGLVIIELDGLHSGTNPISGDFSLGARGFLIENGRIVGGVEGITVSGNILGVLKNI
HHHHCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCEEEECCCEEEECCHHHHHHHHH
VETGNDAKFSLPGITGFSSFAFLPSVLVEEIDVAGL
HHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]