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The map label for this gene is cobS

Identifier: 239616861

GI number: 239616861

Start: 485226

End: 485921

Strand: Direct

Name: cobS

Synonym: Kole_0456

Alternate gene names: 239616861

Gene position: 485226-485921 (Clockwise)

Preceding gene: 239616860

Following gene: 239616862

Centisome position: 21.08

GC content: 38.79

Gene sequence:

>696_bases
TTGTGGAATGACATGAAACTTGCAATAGCATTCCTGAGCAGAGTACCAGTCCCTCTAAAACAAAATAAAGGGAACCTAAA
AAAAATCTGTGCGTATTTCACATTCGTAGGATATCTGGCCGGCGTATTCTATTTTTCTATGAAGCTTATAAGCGAAAACT
TTTTATGGACGCTGTTATCAGTTGCACTTGGATTTTACCTTTTTGATCTATTTCACTTTGATGGTCTCTTAGATACGCTA
GACGGGTTCTTCTATCAGGGAACGAAGGAGCGGCGTTTTGAAATAATGTCAAAAGGAGATATCGGCCCCTTCGCTTTTTT
CTATGCCGCATTGTATATAGTGGCTTATCTTTACGCTTTTTTGCATGTAGATCCAATTGATCTCATTTATGTGGCAGTTC
TCGGAAGATTCTCCATGAATATTCTTTTGCACTTCGGAAAACCAGCAAAAAACACGGGGCTTGGAAAGTTATTACATCCC
TATGAAAAAAAGCATACCCTTATAAGCCTTGTTTTTACCATTCCTCTTGTATACTTTCCTCTAAACTACATCATTTCGTT
ATCTATCGCGCTATTGCTGGGAAGCTCTATGCATTTCATTACAAATAGAAAAATCGAAGGGTATACCGGCGATGTGCTAG
GGGCAACTTGCATGTTTTCCCAGCTTTCAATCATGGTAGCACTTTCGCTAATTTAA

Upstream 100 bases:

>100_bases
GGCTTCATGGGAGATCTTATTACCGAAAATGTTCTTATAAACTTTGAGGTTTTCGATCTTGAAGATTTGAAAAACTCTCT
CAAAGTATGGAGTGATACCT

Downstream 100 bases:

>100_bases
TACGTACTCCCTATAGTCGATAGTTAATCTTCCACAAAAATATTCTCGATTCCATATTTTCGCCTATCCACCTCTGATAT
ATAATCAAGATAGGGGGGCA

Product: cobalamin-5-phosphate synthase CobS

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MWNDMKLAIAFLSRVPVPLKQNKGNLKKICAYFTFVGYLAGVFYFSMKLISENFLWTLLSVALGFYLFDLFHFDGLLDTL
DGFFYQGTKERRFEIMSKGDIGPFAFFYAALYIVAYLYAFLHVDPIDLIYVAVLGRFSMNILLHFGKPAKNTGLGKLLHP
YEKKHTLISLVFTIPLVYFPLNYIISLSIALLLGSSMHFITNRKIEGYTGDVLGATCMFSQLSIMVALSLI

Sequences:

>Translated_231_residues
MWNDMKLAIAFLSRVPVPLKQNKGNLKKICAYFTFVGYLAGVFYFSMKLISENFLWTLLSVALGFYLFDLFHFDGLLDTL
DGFFYQGTKERRFEIMSKGDIGPFAFFYAALYIVAYLYAFLHVDPIDLIYVAVLGRFSMNILLHFGKPAKNTGLGKLLHP
YEKKHTLISLVFTIPLVYFPLNYIISLSIALLLGSSMHFITNRKIEGYTGDVLGATCMFSQLSIMVALSLI
>Mature_231_residues
MWNDMKLAIAFLSRVPVPLKQNKGNLKKICAYFTFVGYLAGVFYFSMKLISENFLWTLLSVALGFYLFDLFHFDGLLDTL
DGFFYQGTKERRFEIMSKGDIGPFAFFYAALYIVAYLYAFLHVDPIDLIYVAVLGRFSMNILLHFGKPAKNTGLGKLLHP
YEKKHTLISLVFTIPLVYFPLNYIISLSIALLLGSSMHFITNRKIEGYTGDVLGATCMFSQLSIMVALSLI

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_KOSOT (C5CE71)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002940183.1
- GeneID:   7967236
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_0456
- ProtClustDB:   CLSK975911
- HAMAP:   MF_00719
- InterPro:   IPR003805

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 26249; Mature: 26249

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x27bae214)-; HASH(0xefdbcf0)-; HASH(0x27d669c0)-; HASH(0x279c8c60)-; HASH(0x2788a71c)-; HASH(0x2805a808)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNDMKLAIAFLSRVPVPLKQNKGNLKKICAYFTFVGYLAGVFYFSMKLISENFLWTLLS
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALGFYLFDLFHFDGLLDTLDGFFYQGTKERRFEIMSKGDIGPFAFFYAALYIVAYLYAF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LHVDPIDLIYVAVLGRFSMNILLHFGKPAKNTGLGKLLHPYEKKHTLISLVFTIPLVYFP
HCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHH
LNYIISLSIALLLGSSMHFITNRKIEGYTGDVLGATCMFSQLSIMVALSLI
HHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MWNDMKLAIAFLSRVPVPLKQNKGNLKKICAYFTFVGYLAGVFYFSMKLISENFLWTLLS
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALGFYLFDLFHFDGLLDTLDGFFYQGTKERRFEIMSKGDIGPFAFFYAALYIVAYLYAF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
LHVDPIDLIYVAVLGRFSMNILLHFGKPAKNTGLGKLLHPYEKKHTLISLVFTIPLVYFP
HCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHH
LNYIISLSIALLLGSSMHFITNRKIEGYTGDVLGATCMFSQLSIMVALSLI
HHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA