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The map label for this gene is rpfG [H]

Identifier: 239616857

GI number: 239616857

Start: 480827

End: 483121

Strand: Direct

Name: rpfG [H]

Synonym: Kole_0452

Alternate gene names: 239616857

Gene position: 480827-483121 (Clockwise)

Preceding gene: 239616854

Following gene: 239616858

Centisome position: 20.89

GC content: 39.13

Gene sequence:

>2295_bases
ATGGTACCACCATTGGAACTTGGGAGGCCCGCTTTTTTTCGTGACACCCTATCCGTCTTATTCACTCAAGAATATTGTTT
TGCTACTAAAGAAGGAAACGATCAGCTTTTAAGTAGCCTCAACGAAGGGCTGGCTATAATCAAAGAGACAGGTAAATACA
AAGAGATCTATGAAAATTGGTTTGGGACGTTGTCAGCACAACATCTTATAATCAAAAATTTAACAAAGTACGGATCGATA
ATCTTGTTGATTTTAACTGTTGGATTTTTGCTTGCCTTGAGCTGGTCGAGAGTTTTAAAGATAAGAGTAGAAGAAAAAAC
AAAAGAATTACGAAAAGAACTGCTCGAAAGAATGATTACTGAAGAAAAACTCCGCGTTAGTGAAGAGCGATACCGCAAGC
TTTTCGAGAGTATGCTCGATGGTTTTATTCTCCTTAAACCCGTGGCAGATAAAGATGGTAATATAAACAATTTCAAGATC
ATCGAAGCAAATACCTCTTTTTTGAAAGCAGTAGGCAAGAAGAAAGAAGAAGTTGTGGGGAAAATGCTCTGGGACATATT
TCCAACGGTTTACAAATCCCACATAAAAGTCTATGAAAAAGTTATAGAAACAGGAAGACCAACACATTTTGAAATCCATT
CAGATGAAACAAACAAAACATTTGAGATAGTCGGATTCACTCCGCAGACTGGTTATTTTGCTGCAATCGTAAGAGATATA
ACCCAAAGAAAGCAGGCTGAAAAAGCCCTGAAAGATAATCTGGAAAAAATCAGGAAACTACATGAAATAGCGTTAAAAAT
GGAAAAGAGTAAATCTGAAGAAGAAGTATATCACTTAATTGTCGAAGCAGCTAAAAAGATACTTGAGTTCGACATTTACA
GTCTCGACATAGTTGAAGGAAATAAATTCGTGGTTAAAGCAATATCTGAAAAAGTTGCAAGTCCGGAAATCATGGAGGCT
CCCCTAAATGAGGGTATAGCCGGAAAAACTTTCCGTACAGGCAAAACCATCGTGGTAAAAGATATCGCTAATGAACCGGA
AGCGAAACCCCGGAGTAAAGAATACCGTTCAGCTATAAGCATACCAATAGGCAATATAGGTGTTTTCCAGACAATCTCAA
CGAAGAGGAACGCTTTCAGCAAAGAAGATGTTGAACTTGCGGAACTACTCATTTTCCATGCCACAGAAGCAATTGAACGA
ATTCGCTCAGAGGCAAAGATAAAATATCTCATCTTTCACGATCACCTGACAGGGGTTTACAATCGAGCATTCTTCGAAGA
GGAGTTAAAACGCTTTGACGCTGAATCCCATCTTCCCCTTAGCATAATCATGGGAGATGTAAACGGCCTTAAACTCGTAA
ACGACGCTTTTGGACATACTGAGGGAGATCGTTTGTTAAAAACCGTCGCCACATCAATAATCTCCTCTTGCAGGCCACAA
GACATTGTAGCTCGATGGGGCGGAGATGAATTTATAATCTTATTGCCGAGGGCCGGTTATATGCTCGCCGAAGGAATATT
GAAACGAATCAAGATGAATCTGGAAAAATCTGACGAGGGTTCATATATCCCTATTAGCGTAGCTTTTGGAATTGCGACTA
AAGAAAATGCCAACCAAAATATTCAAGAAATTCTGAAAAAAGTCGAAGAGCAAATGTATAAAAACAAACTTTTGAAAAGC
ATGAGCACCAGAAATTCAATAATAACTTCCCTTGAAAAAATGTTACTCGAAAAGAGCAATGAAACCGAAGAGCACGCAAG
GCGCATGAGAAAAATAGCCTATGAATTCGGAAAGTATATCAATCTCACGGACAGTGAGATCAACGATTTAGTTTTGCTGG
CTGCACTTCACGATATTGGAAAGATATCCGTACCTGAATCAATACTGAAGAAACCTGGTCCACTGACAGTCGAAGAATGG
GAAAAAATAAAAGAGCATCCGGAAACAGGTTATCGTATAGCAATAAACTCGCCAGACCTTGTTACCATAGCAGATGGTAT
CCTGTACCACCACGAATGGTGGAATGGGAGCGGTTATCCACGAGGGCTTAAAGAGGAAGAAATACCGTTTATAGCGAGAG
TAATTTCAATAATCGATGCGTACGATGTTATGACTTCTGGACGCCCTTATAAGAAACGGCTGAGCAAAGATAAAGCCCTC
GAAGAAATAAAGAAATCTTCGGGTATCCAGTTTGATCCCAGGCTTGCAAGGTTATTCATGGAGTTCATGCAAAGTTTGGA
AGACTCTGAAAAATCTGATAGAAATTCTGATAAAGATAAAAGTTATCATGAGTAA

Upstream 100 bases:

>100_bases
AAAGCTTGTCTAACTGCTCAATAATCTTTACAATATCGCTTTTTGTGAGTTCTTGTGGTACCATTTATTTGGGCCTCCTT
TCTTGTTTTGGGCTATTTCA

Downstream 100 bases:

>100_bases
GGAGGTAAAAAATGGCAAAAAAAGATCTTGAAAACAAAATAAAAGAAAGACTGAATAGCCTGACAAAACCGAAAGGAAGC
CTTGGCTTCCTCGAGGATAT

Product: diguanylate cyclase and metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MVPPLELGRPAFFRDTLSVLFTQEYCFATKEGNDQLLSSLNEGLAIIKETGKYKEIYENWFGTLSAQHLIIKNLTKYGSI
ILLILTVGFLLALSWSRVLKIRVEEKTKELRKELLERMITEEKLRVSEERYRKLFESMLDGFILLKPVADKDGNINNFKI
IEANTSFLKAVGKKKEEVVGKMLWDIFPTVYKSHIKVYEKVIETGRPTHFEIHSDETNKTFEIVGFTPQTGYFAAIVRDI
TQRKQAEKALKDNLEKIRKLHEIALKMEKSKSEEEVYHLIVEAAKKILEFDIYSLDIVEGNKFVVKAISEKVASPEIMEA
PLNEGIAGKTFRTGKTIVVKDIANEPEAKPRSKEYRSAISIPIGNIGVFQTISTKRNAFSKEDVELAELLIFHATEAIER
IRSEAKIKYLIFHDHLTGVYNRAFFEEELKRFDAESHLPLSIIMGDVNGLKLVNDAFGHTEGDRLLKTVATSIISSCRPQ
DIVARWGGDEFIILLPRAGYMLAEGILKRIKMNLEKSDEGSYIPISVAFGIATKENANQNIQEILKKVEEQMYKNKLLKS
MSTRNSIITSLEKMLLEKSNETEEHARRMRKIAYEFGKYINLTDSEINDLVLLAALHDIGKISVPESILKKPGPLTVEEW
EKIKEHPETGYRIAINSPDLVTIADGILYHHEWWNGSGYPRGLKEEEIPFIARVISIIDAYDVMTSGRPYKKRLSKDKAL
EEIKKSSGIQFDPRLARLFMEFMQSLEDSEKSDRNSDKDKSYHE

Sequences:

>Translated_764_residues
MVPPLELGRPAFFRDTLSVLFTQEYCFATKEGNDQLLSSLNEGLAIIKETGKYKEIYENWFGTLSAQHLIIKNLTKYGSI
ILLILTVGFLLALSWSRVLKIRVEEKTKELRKELLERMITEEKLRVSEERYRKLFESMLDGFILLKPVADKDGNINNFKI
IEANTSFLKAVGKKKEEVVGKMLWDIFPTVYKSHIKVYEKVIETGRPTHFEIHSDETNKTFEIVGFTPQTGYFAAIVRDI
TQRKQAEKALKDNLEKIRKLHEIALKMEKSKSEEEVYHLIVEAAKKILEFDIYSLDIVEGNKFVVKAISEKVASPEIMEA
PLNEGIAGKTFRTGKTIVVKDIANEPEAKPRSKEYRSAISIPIGNIGVFQTISTKRNAFSKEDVELAELLIFHATEAIER
IRSEAKIKYLIFHDHLTGVYNRAFFEEELKRFDAESHLPLSIIMGDVNGLKLVNDAFGHTEGDRLLKTVATSIISSCRPQ
DIVARWGGDEFIILLPRAGYMLAEGILKRIKMNLEKSDEGSYIPISVAFGIATKENANQNIQEILKKVEEQMYKNKLLKS
MSTRNSIITSLEKMLLEKSNETEEHARRMRKIAYEFGKYINLTDSEINDLVLLAALHDIGKISVPESILKKPGPLTVEEW
EKIKEHPETGYRIAINSPDLVTIADGILYHHEWWNGSGYPRGLKEEEIPFIARVISIIDAYDVMTSGRPYKKRLSKDKAL
EEIKKSSGIQFDPRLARLFMEFMQSLEDSEKSDRNSDKDKSYHE
>Mature_764_residues
MVPPLELGRPAFFRDTLSVLFTQEYCFATKEGNDQLLSSLNEGLAIIKETGKYKEIYENWFGTLSAQHLIIKNLTKYGSI
ILLILTVGFLLALSWSRVLKIRVEEKTKELRKELLERMITEEKLRVSEERYRKLFESMLDGFILLKPVADKDGNINNFKI
IEANTSFLKAVGKKKEEVVGKMLWDIFPTVYKSHIKVYEKVIETGRPTHFEIHSDETNKTFEIVGFTPQTGYFAAIVRDI
TQRKQAEKALKDNLEKIRKLHEIALKMEKSKSEEEVYHLIVEAAKKILEFDIYSLDIVEGNKFVVKAISEKVASPEIMEA
PLNEGIAGKTFRTGKTIVVKDIANEPEAKPRSKEYRSAISIPIGNIGVFQTISTKRNAFSKEDVELAELLIFHATEAIER
IRSEAKIKYLIFHDHLTGVYNRAFFEEELKRFDAESHLPLSIIMGDVNGLKLVNDAFGHTEGDRLLKTVATSIISSCRPQ
DIVARWGGDEFIILLPRAGYMLAEGILKRIKMNLEKSDEGSYIPISVAFGIATKENANQNIQEILKKVEEQMYKNKLLKS
MSTRNSIITSLEKMLLEKSNETEEHARRMRKIAYEFGKYINLTDSEINDLVLLAALHDIGKISVPESILKKPGPLTVEEW
EKIKEHPETGYRIAINSPDLVTIADGILYHHEWWNGSGYPRGLKEEEIPFIARVISIIDAYDVMTSGRPYKKRLSKDKAL
EEIKKSSGIQFDPRLARLFMEFMQSLEDSEKSDRNSDKDKSYHE

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=31.3953488372093, Blast_Score=93, Evalue=8e-20,
Organism=Escherichia coli, GI87082007, Length=175, Percent_Identity=30.8571428571429, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787262, Length=150, Percent_Identity=36, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788381, Length=174, Percent_Identity=31.0344827586207, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI145693134, Length=147, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=27.4853801169591, Blast_Score=73, Evalue=8e-14,
Organism=Escherichia coli, GI87081974, Length=151, Percent_Identity=34.4370860927152, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 87415; Mature: 87415

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS50112 PAS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPPLELGRPAFFRDTLSVLFTQEYCFATKEGNDQLLSSLNEGLAIIKETGKYKEIYENW
CCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
FGTLSAQHLIIKNLTKYGSIILLILTVGFLLALSWSRVLKIRVEEKTKELRKELLERMIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHH
EEKLRVSEERYRKLFESMLDGFILLKPVADKDGNINNFKIIEANTSFLKAVGKKKEEVVG
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHCCHHHHHHH
KMLWDIFPTVYKSHIKVYEKVIETGRPTHFEIHSDETNKTFEIVGFTPQTGYFAAIVRDI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHH
TQRKQAEKALKDNLEKIRKLHEIALKMEKSKSEEEVYHLIVEAAKKILEFDIYSLDIVEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEEECC
NKFVVKAISEKVASPEIMEAPLNEGIAGKTFRTGKTIVVKDIANEPEAKPRSKEYRSAIS
CHHHHHHHHHHHCCCHHHHCCCCCCCCCCEECCCCEEEEEECCCCCCCCCCCHHHHHHHC
IPIGNIGVFQTISTKRNAFSKEDVELAELLIFHATEAIERIRSEAKIKYLIFHDHLTGVY
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHH
NRAFFEEELKRFDAESHLPLSIIMGDVNGLKLVNDAFGHTEGDRLLKTVATSIISSCRPQ
HHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCH
DIVARWGGDEFIILLPRAGYMLAEGILKRIKMNLEKSDEGSYIPISVAFGIATKENANQN
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCHH
IQEILKKVEEQMYKNKLLKSMSTRNSIITSLEKMLLEKSNETEEHARRMRKIAYEFGKYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEE
NLTDSEINDLVLLAALHDIGKISVPESILKKPGPLTVEEWEKIKEHPETGYRIAINSPDL
ECCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHCCCCCCEEEEECCCCE
VTIADGILYHHEWWNGSGYPRGLKEEEIPFIARVISIIDAYDVMTSGRPYKKRLSKDKAL
EEEEHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EEIKKSSGIQFDPRLARLFMEFMQSLEDSEKSDRNSDKDKSYHE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MVPPLELGRPAFFRDTLSVLFTQEYCFATKEGNDQLLSSLNEGLAIIKETGKYKEIYENW
CCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
FGTLSAQHLIIKNLTKYGSIILLILTVGFLLALSWSRVLKIRVEEKTKELRKELLERMIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHH
EEKLRVSEERYRKLFESMLDGFILLKPVADKDGNINNFKIIEANTSFLKAVGKKKEEVVG
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHCCHHHHHHH
KMLWDIFPTVYKSHIKVYEKVIETGRPTHFEIHSDETNKTFEIVGFTPQTGYFAAIVRDI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHH
TQRKQAEKALKDNLEKIRKLHEIALKMEKSKSEEEVYHLIVEAAKKILEFDIYSLDIVEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEEECC
NKFVVKAISEKVASPEIMEAPLNEGIAGKTFRTGKTIVVKDIANEPEAKPRSKEYRSAIS
CHHHHHHHHHHHCCCHHHHCCCCCCCCCCEECCCCEEEEEECCCCCCCCCCCHHHHHHHC
IPIGNIGVFQTISTKRNAFSKEDVELAELLIFHATEAIERIRSEAKIKYLIFHDHLTGVY
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHH
NRAFFEEELKRFDAESHLPLSIIMGDVNGLKLVNDAFGHTEGDRLLKTVATSIISSCRPQ
HHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCH
DIVARWGGDEFIILLPRAGYMLAEGILKRIKMNLEKSDEGSYIPISVAFGIATKENANQN
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCHH
IQEILKKVEEQMYKNKLLKSMSTRNSIITSLEKMLLEKSNETEEHARRMRKIAYEFGKYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEE
NLTDSEINDLVLLAALHDIGKISVPESILKKPGPLTVEEWEKIKEHPETGYRIAINSPDL
ECCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHCCCCCCEEEEECCCCE
VTIADGILYHHEWWNGSGYPRGLKEEEIPFIARVISIIDAYDVMTSGRPYKKRLSKDKAL
EEEEHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EEIKKSSGIQFDPRLARLFMEFMQSLEDSEKSDRNSDKDKSYHE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]