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The map label for this gene is aspA [H]

Identifier: 239616805

GI number: 239616805

Start: 417082

End: 418476

Strand: Direct

Name: aspA [H]

Synonym: Kole_0398

Alternate gene names: 239616805

Gene position: 417082-418476 (Clockwise)

Preceding gene: 239616804

Following gene: 239616806

Centisome position: 18.12

GC content: 41.51

Gene sequence:

>1395_bases
ATGAGAATTGAAAAAGATGCACTCGGAAAACTTGAAATTCCCGATGATGTTTACTGGGGAATTCATACGGCCAGGGCATT
AAAGAACTTTCCATCTACCGGTGAAAAAATTGACGAGGAATTTATCAGATGCTACGGTTATATAAAAAAATCGGCAGCCT
TCCTCAATAAAAAACTTGGATTTCTTGAGACAAAAATCGCTGATGCTGTCATACAAGCTGCTGATGAGCTTATATCAGGT
TCTTTGATGAAATGGGTTGTTGTGGATCCGTTATGTGGGGGTGCTGGTACCTCAATAAACATGAATATCAATGAGGTTAT
AGCTAACCGCGCCACGGAGATACTTGGAGGAAAACTAGGAGAGTACATTGTCCACCCATTGGATCATGTGAACCTTCATC
AATCTACAAATGATACCTTTCCGACAGCCGGCAAAATGGCTACAATAGCCCTCCTAAAACAGCTGGTTGAGAATGTTACT
AACCTTCAGGACAGCCTCCAGAAAAAGGAAGCTGAATACAGAAAAATGATAAAACCTGCGCGAACCCAGCTCATGGATGC
AGTACCGATTTCTTTGGGGCAGGAGTTTGGCGCCATGGCAGAAGCCATTAGCCGCGATCGCTGGAGACTTTACAAAGTTG
AAGAACGCATCAGAATGGTGAATCTTGGCGGAACGGCAGTAGGTACTGGAATAGCAGCAGATCGAAAATACGTTCTCTCG
ATAGTTGAAACGTTACGAACCGTTTCAGGCATAAAGATAGCTAAAGCGGAGAACCTGATAGATGCCACCCAAAATATGGA
CGTCTTTGCTGAAATTCATGGTTTGCTAAAAGCCCTGGCTACAAACCTCATAAAAATATCTAATGACATAAGATTACTTG
GAAGCGGCCCAGGTTCAGCTATCGGAGAGATAAAACTTCCAAAGCTGCAGGCTGGGAGTTCTATAATGCCTGGTAAGATA
AACCCGGTAATTCCAGAATATGTTGTACAACTATCATTAGCGGTTCTGGGGCATGATGCCATCATTAACTTCGCGGTTTC
TTCAGGAAATTTAGAACTAAATGCCTTTGTTCCCGAGATTATTCATTACACTCTCAAATCTTTAAGATTCCTCACAATAG
CCGCAAATTCACTAGCAAACTATATTTTGAAAATAGAAGCCAATCCCGAAAAAATGAGAGAAAACTTGCTTAGCAGTTCC
GCAATACTGACTCCGCTCATAACGACCTATGGTTATGATAAAGTACAGGAGTGGATAGAGTTATCAAGCAAAACCGGAAA
GAATTTGTGGGAAATCGTTAAGGATTCCCTGGGAGAGGAAGCTGAAAAATTCAAAGCAAAACTTATTTCCAATAAATCCA
CTGGTATTGGTTTTTCACACCAAGACTTTGAGTAA

Upstream 100 bases:

>100_bases
GGAGTGTTGATATCTTACTTACACGGTGTTCTTGAAAGAGTTCTAAGGCCGTTCCCTGACGCCCATACGATTTATATCGA
GGAGATTGAAGGTGTAACTC

Downstream 100 bases:

>100_bases
AAGGAAAAACATGTTATTATTTCCTTGAAAAAGCTTCTATGCTCTACGGAGGTATTTCAATGAACAAAATTCATATAGCT
CTTATAGCTCACGATAAGAA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MRIEKDALGKLEIPDDVYWGIHTARALKNFPSTGEKIDEEFIRCYGYIKKSAAFLNKKLGFLETKIADAVIQAADELISG
SLMKWVVVDPLCGGAGTSINMNINEVIANRATEILGGKLGEYIVHPLDHVNLHQSTNDTFPTAGKMATIALLKQLVENVT
NLQDSLQKKEAEYRKMIKPARTQLMDAVPISLGQEFGAMAEAISRDRWRLYKVEERIRMVNLGGTAVGTGIAADRKYVLS
IVETLRTVSGIKIAKAENLIDATQNMDVFAEIHGLLKALATNLIKISNDIRLLGSGPGSAIGEIKLPKLQAGSSIMPGKI
NPVIPEYVVQLSLAVLGHDAIINFAVSSGNLELNAFVPEIIHYTLKSLRFLTIAANSLANYILKIEANPEKMRENLLSSS
AILTPLITTYGYDKVQEWIELSSKTGKNLWEIVKDSLGEEAEKFKAKLISNKSTGIGFSHQDFE

Sequences:

>Translated_464_residues
MRIEKDALGKLEIPDDVYWGIHTARALKNFPSTGEKIDEEFIRCYGYIKKSAAFLNKKLGFLETKIADAVIQAADELISG
SLMKWVVVDPLCGGAGTSINMNINEVIANRATEILGGKLGEYIVHPLDHVNLHQSTNDTFPTAGKMATIALLKQLVENVT
NLQDSLQKKEAEYRKMIKPARTQLMDAVPISLGQEFGAMAEAISRDRWRLYKVEERIRMVNLGGTAVGTGIAADRKYVLS
IVETLRTVSGIKIAKAENLIDATQNMDVFAEIHGLLKALATNLIKISNDIRLLGSGPGSAIGEIKLPKLQAGSSIMPGKI
NPVIPEYVVQLSLAVLGHDAIINFAVSSGNLELNAFVPEIIHYTLKSLRFLTIAANSLANYILKIEANPEKMRENLLSSS
AILTPLITTYGYDKVQEWIELSSKTGKNLWEIVKDSLGEEAEKFKAKLISNKSTGIGFSHQDFE
>Mature_464_residues
MRIEKDALGKLEIPDDVYWGIHTARALKNFPSTGEKIDEEFIRCYGYIKKSAAFLNKKLGFLETKIADAVIQAADELISG
SLMKWVVVDPLCGGAGTSINMNINEVIANRATEILGGKLGEYIVHPLDHVNLHQSTNDTFPTAGKMATIALLKQLVENVT
NLQDSLQKKEAEYRKMIKPARTQLMDAVPISLGQEFGAMAEAISRDRWRLYKVEERIRMVNLGGTAVGTGIAADRKYVLS
IVETLRTVSGIKIAKAENLIDATQNMDVFAEIHGLLKALATNLIKISNDIRLLGSGPGSAIGEIKLPKLQAGSSIMPGKI
NPVIPEYVVQLSLAVLGHDAIINFAVSSGNLELNAFVPEIIHYTLKSLRFLTIAANSLANYILKIEANPEKMRENLLSSS
AILTPLITTYGYDKVQEWIELSSKTGKNLWEIVKDSLGEEAEKFKAKLISNKSTGIGFSHQDFE

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=449, Percent_Identity=38.5300668151448, Blast_Score=294, Evalue=1e-79,
Organism=Escherichia coli, GI87082375, Length=438, Percent_Identity=41.324200913242, Blast_Score=308, Evalue=6e-85,
Organism=Escherichia coli, GI1787896, Length=429, Percent_Identity=39.1608391608392, Blast_Score=298, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI17553882, Length=436, Percent_Identity=38.5321100917431, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI32565146, Length=302, Percent_Identity=34.4370860927152, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6324993, Length=435, Percent_Identity=40, Blast_Score=312, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24640179, Length=439, Percent_Identity=39.6355353075171, Blast_Score=308, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24640177, Length=439, Percent_Identity=39.6355353075171, Blast_Score=308, Evalue=4e-84,
Organism=Drosophila melanogaster, GI24662684, Length=441, Percent_Identity=40.1360544217687, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI78710009, Length=422, Percent_Identity=39.8104265402844, Blast_Score=297, Evalue=9e-81,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=35.85313174946, Blast_Score=267, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 50937; Mature: 50937

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIEKDALGKLEIPDDVYWGIHTARALKNFPSTGEKIDEEFIRCYGYIKKSAAFLNKKLG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FLETKIADAVIQAADELISGSLMKWVVVDPLCGGAGTSINMNINEVIANRATEILGGKLG
HHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCEEECCHHHHHHHHHHHHHCCHHH
EYIVHPLDHVNLHQSTNDTFPTAGKMATIALLKQLVENVTNLQDSLQKKEAEYRKMIKPA
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTQLMDAVPISLGQEFGAMAEAISRDRWRLYKVEERIRMVNLGGTAVGTGIAADRKYVLS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCCHHCCCCHHHHHHHHH
IVETLRTVSGIKIAKAENLIDATQNMDVFAEIHGLLKALATNLIKISNDIRLLGSGPGSA
HHHHHHHHCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
IGEIKLPKLQAGSSIMPGKINPVIPEYVVQLSLAVLGHDAIINFAVSSGNLELNAFVPEI
CCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHEEEEEECCCEEEHHHHHHH
IHYTLKSLRFLTIAANSLANYILKIEANPEKMRENLLSSSAILTPLITTYGYDKVQEWIE
HHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LSSKTGKNLWEIVKDSLGEEAEKFKAKLISNKSTGIGFSHQDFE
HHHCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRIEKDALGKLEIPDDVYWGIHTARALKNFPSTGEKIDEEFIRCYGYIKKSAAFLNKKLG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FLETKIADAVIQAADELISGSLMKWVVVDPLCGGAGTSINMNINEVIANRATEILGGKLG
HHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCEEECCHHHHHHHHHHHHHCCHHH
EYIVHPLDHVNLHQSTNDTFPTAGKMATIALLKQLVENVTNLQDSLQKKEAEYRKMIKPA
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTQLMDAVPISLGQEFGAMAEAISRDRWRLYKVEERIRMVNLGGTAVGTGIAADRKYVLS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCCHHCCCCHHHHHHHHH
IVETLRTVSGIKIAKAENLIDATQNMDVFAEIHGLLKALATNLIKISNDIRLLGSGPGSA
HHHHHHHHCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
IGEIKLPKLQAGSSIMPGKINPVIPEYVVQLSLAVLGHDAIINFAVSSGNLELNAFVPEI
CCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHEEEEEECCCEEEHHHHHHH
IHYTLKSLRFLTIAANSLANYILKIEANPEKMRENLLSSSAILTPLITTYGYDKVQEWIE
HHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LSSKTGKNLWEIVKDSLGEEAEKFKAKLISNKSTGIGFSHQDFE
HHHCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]