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The map label for this gene is mnmE [H]

Identifier: 239616804

GI number: 239616804

Start: 415863

End: 417092

Strand: Direct

Name: mnmE [H]

Synonym: Kole_0397

Alternate gene names: 239616804

Gene position: 415863-417092 (Clockwise)

Preceding gene: 239616803

Following gene: 239616805

Centisome position: 18.06

GC content: 44.72

Gene sequence:

>1230_bases
ATGATTGCTACCGGTGGTTATAGAAAGTATCTTGCATTCTTCGGAAAGCGAAACGTTGGAAAGTCGAGTTTGATAAACGC
TCTCGTTGGACAGGATGTGTCTATTGTAAGTGATGTCCCGGGAACAACAACTGACCCGGTTTATAAATCATTAGAGATAC
ACCCGATAGGTCCTGTAACGGTTGTCGATACCCCGGGTGTGGATGATGTCGGTGAGCTCGGTGAACTCAGGGTGAAAAGG
GCCCTAAAAGCCATGTACAAGGCAGATATTGCCATTTTGGTTGTAGATCAACGCTGGAACGAATACGAGGATTGGCTGAT
AGAACAGTTCATTAAAATGGATTTACCATTCGTGATAGCAGTAAACAAAATAGATCAATCCTGGAACACTTCGGAGATAG
AACCAAAGGTTTCTCCCTACCCTCATTGTTTCGTATCAGGACTCAAAAAAACTGGTATTGAGGATCTTCGAAGACTCATC
AGTGAAAATCTTCCACAGGAAGAGGATATACCGCTGCTTTCCGATTTAATTGAAGCTGGTGAAACTGTCCTTCTGGTCGT
TCCAATAGATCTCGGTGCACCTAAAGGCAGACTTATTATGCCGCAAGTTGAAGCTATCAGAGAGGTCCTGGATAGAGAAG
CACTATGTCTCGTGGTTAAGGAAAGGGAACTACGCTGGGCTCTACAAATGATGAAAGAGGCACCTTCTCTGGTTGTCGCC
GACTCACATCTTATAAGCAGAATCGCAGCAGATGTGCCGGATGAAATTCCACTGACCACTTTTTCCATACTGGAAGCCAG
GCACAAAGGTGATCTTGTTGAGATGGTCAAGGGAATCAAACGAGTGGAAGAACTCACGCCTGAGAGCAAGATAATGATCA
TGGAAGGTTGTTCCCATGCTCCTCTTGTCGAGGATATCGGAAGGGTCAAAATACCTCGATGGCTTTCGAACCATATTGGC
GGTGGCCTTTCTTTTGAAATAAGGGCAGGAAGGGATTTTCCCGAAGATATAGGAGAGATAGATCTTATTATCCACTGTGG
TGGCTGTACTTTAACAAGAGCCATGATGATGCGGAGAATCAGAGAAGCCCGTCGGCGTGGAATACCAATAGTGAATTATG
GAGTGTTGATATCTTACTTACACGGTGTTCTTGAAAGAGTTCTAAGGCCGTTCCCTGACGCCCATACGATTTATATCGAG
GAGATTGAAGGTGTAACTCATGAGAATTGA

Upstream 100 bases:

>100_bases
AAGTTGATTTCTTTAAAACAGCAAAAAGAATGAAAGATATGGGCCTCAAGCTAAATCCAAGGATTCTGAAAAACCTTGAG
GAATACAAGAAGGTGATCAT

Downstream 100 bases:

>100_bases
AAAAGATGCACTCGGAAAACTTGAAATTCCCGATGATGTTTACTGGGGAATTCATACGGCCAGGGCATTAAAGAACTTTC
CATCTACCGGTGAAAAAATT

Product: small GTP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MIATGGYRKYLAFFGKRNVGKSSLINALVGQDVSIVSDVPGTTTDPVYKSLEIHPIGPVTVVDTPGVDDVGELGELRVKR
ALKAMYKADIAILVVDQRWNEYEDWLIEQFIKMDLPFVIAVNKIDQSWNTSEIEPKVSPYPHCFVSGLKKTGIEDLRRLI
SENLPQEEDIPLLSDLIEAGETVLLVVPIDLGAPKGRLIMPQVEAIREVLDREALCLVVKERELRWALQMMKEAPSLVVA
DSHLISRIAADVPDEIPLTTFSILEARHKGDLVEMVKGIKRVEELTPESKIMIMEGCSHAPLVEDIGRVKIPRWLSNHIG
GGLSFEIRAGRDFPEDIGEIDLIIHCGGCTLTRAMMMRRIREARRRGIPIVNYGVLISYLHGVLERVLRPFPDAHTIYIE
EIEGVTHEN

Sequences:

>Translated_409_residues
MIATGGYRKYLAFFGKRNVGKSSLINALVGQDVSIVSDVPGTTTDPVYKSLEIHPIGPVTVVDTPGVDDVGELGELRVKR
ALKAMYKADIAILVVDQRWNEYEDWLIEQFIKMDLPFVIAVNKIDQSWNTSEIEPKVSPYPHCFVSGLKKTGIEDLRRLI
SENLPQEEDIPLLSDLIEAGETVLLVVPIDLGAPKGRLIMPQVEAIREVLDREALCLVVKERELRWALQMMKEAPSLVVA
DSHLISRIAADVPDEIPLTTFSILEARHKGDLVEMVKGIKRVEELTPESKIMIMEGCSHAPLVEDIGRVKIPRWLSNHIG
GGLSFEIRAGRDFPEDIGEIDLIIHCGGCTLTRAMMMRRIREARRRGIPIVNYGVLISYLHGVLERVLRPFPDAHTIYIE
EIEGVTHEN
>Mature_409_residues
MIATGGYRKYLAFFGKRNVGKSSLINALVGQDVSIVSDVPGTTTDPVYKSLEIHPIGPVTVVDTPGVDDVGELGELRVKR
ALKAMYKADIAILVVDQRWNEYEDWLIEQFIKMDLPFVIAVNKIDQSWNTSEIEPKVSPYPHCFVSGLKKTGIEDLRRLI
SENLPQEEDIPLLSDLIEAGETVLLVVPIDLGAPKGRLIMPQVEAIREVLDREALCLVVKERELRWALQMMKEAPSLVVA
DSHLISRIAADVPDEIPLTTFSILEARHKGDLVEMVKGIKRVEELTPESKIMIMEGCSHAPLVEDIGRVKIPRWLSNHIG
GGLSFEIRAGRDFPEDIGEIDLIIHCGGCTLTRAMMMRRIREARRRGIPIVNYGVLISYLHGVLERVLRPFPDAHTIYIE
EIEGVTHEN

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG1160

COG function: function code R; Predicted GTPases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 45802; Mature: 45802

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATGGYRKYLAFFGKRNVGKSSLINALVGQDVSIVSDVPGTTTDPVYKSLEIHPIGPVT
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCHHHCEEEECCCCEE
VVDTPGVDDVGELGELRVKRALKAMYKADIAILVVDQRWNEYEDWLIEQFIKMDLPFVIA
EECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEE
VNKIDQSWNTSEIEPKVSPYPHCFVSGLKKTGIEDLRRLISENLPQEEDIPLLSDLIEAG
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHCC
ETVLLVVPIDLGAPKGRLIMPQVEAIREVLDREALCLVVKERELRWALQMMKEAPSLVVA
CEEEEEEEECCCCCCCCEECHHHHHHHHHHCCHHEEEEEHHHHHHHHHHHHHHCCCEEEE
DSHLISRIAADVPDEIPLTTFSILEARHKGDLVEMVKGIKRVEELTPESKIMIMEGCSHA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCC
PLVEDIGRVKIPRWLSNHIGGGLSFEIRAGRDFPEDIGEIDLIIHCGGCTLTRAMMMRRI
CHHHHHCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHH
REARRRGIPIVNYGVLISYLHGVLERVLRPFPDAHTIYIEEIEGVTHEN
HHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MIATGGYRKYLAFFGKRNVGKSSLINALVGQDVSIVSDVPGTTTDPVYKSLEIHPIGPVT
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCHHHCEEEECCCCEE
VVDTPGVDDVGELGELRVKRALKAMYKADIAILVVDQRWNEYEDWLIEQFIKMDLPFVIA
EECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEE
VNKIDQSWNTSEIEPKVSPYPHCFVSGLKKTGIEDLRRLISENLPQEEDIPLLSDLIEAG
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHCC
ETVLLVVPIDLGAPKGRLIMPQVEAIREVLDREALCLVVKERELRWALQMMKEAPSLVVA
CEEEEEEEECCCCCCCCEECHHHHHHHHHHCCHHEEEEEHHHHHHHHHHHHHHCCCEEEE
DSHLISRIAADVPDEIPLTTFSILEARHKGDLVEMVKGIKRVEELTPESKIMIMEGCSHA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCC
PLVEDIGRVKIPRWLSNHIGGGLSFEIRAGRDFPEDIGEIDLIIHCGGCTLTRAMMMRRI
CHHHHHCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHH
REARRRGIPIVNYGVLISYLHGVLERVLRPFPDAHTIYIEEIEGVTHEN
HHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]