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The map label for this gene is gatB [H]

Identifier: 239616732

GI number: 239616732

Start: 344903

End: 346348

Strand: Reverse

Name: gatB [H]

Synonym: Kole_0325

Alternate gene names: 239616732

Gene position: 346348-344903 (Counterclockwise)

Preceding gene: 239616733

Following gene: 239616731

Centisome position: 15.04

GC content: 41.98

Gene sequence:

>1446_bases
ATGTATAAAGCAGTTATAGGTTTGGAGATACATGCCCAGCTCTCAACAGTGACAAAGGCCTTTTGTAATTGTAGCGCCGA
TGTCTTCGAATTGGAACCTAATACTGCAATCTGCCCGGTTTGTACCGGGCAACCGGGATCGTTACCGGTCCTGAACGAAA
AAGTTGTGGAATATGCTTTGAAAGCTGCACTGGCTTTTAATTGTGAGATTAACCTTTTCTCTTCCTTTGATCGAAAAAAT
TACTTTTACCCGGATCTTCCCAAGGGGTACCAGATAACGCAGTATTTTCATCCAATAGCAGAACATGGTTATCTTATGAT
AGGAACAGGTGAAAGTAGAAAGAAGGTACGAATAAGGAGAATACATATAGAAGAAGATGCCGGAAAAATGCTCCATCAGG
GAGCAGATTCCATATCTGGAGCCACGGAAAGTTTTGTCGATTTGAATCGTTGTGGTGTGCCGCTTATAGAAATTGTCACG
GAGCCGGATTTGAATTCTCCAAAAGAAGCCAGGGAGTTTATGGAGCTTTTGAGAGATACCCTTAGATACATAGAGGTATG
CAGTGGAGATATGGAAAAAGGAGCTTTACGTTGTGATGCCAATATATCCGTTTACGACACAGAGACAGGTAAATCCTCGG
AAAGGGTGGAAGTAAAGAACATAAATTCTTTTAAGTTTGTTGAAAAAGCTCTGGAGTTTGAATTTGAGAGAATAAAAGCA
GCTCTGGAAAACGGCGAAAATATTGTACGGGAAACCAGAAGCTGGAACTTTTCTGCGCGGGAAACCACTTCAATGAGATC
CAAAGAAGAGGAGAATGATTACAGATACTTTCCGGAGCCAGATCTTCCGGAACTGAAATTGACGCAGGAATATGTGGATA
AAGTGAAAAAAGAATTGCCGGAACTTCCATGGGAAAAACAGAGTCGATTTGTGCAGGAGTACGGTATCCCTGAGTACGAC
GCGTCAGTACTGTGCTCGGACAAAGAACTTGCCAATTTCTTTGAGGATGTTGCGAGAGCCACCGGAAAACCAAAAGAAAC
ATCAAACTGGATAATGACGGAGTTTTTAAGAGAGCTAAAGCAGCGGAACGCTTCCGTAGATCAGCTTGGTATCAAGCCAG
AGCATTTCGTCGATCTCTTTAAACTTCTGGATTCAGGAAAAATTTCCACGAAAATAGCGAAAGAGCTCTTCTCAAAAATG
GTAGAGAACAAAAAAATGCCATCAGAACTGGTTGAAGAACTTGGTTTACAGCAGATAAACGATGAAAAGTTGATAGAAGA
GCTTATAAGAAAAGCCATGGAGGCAAATCCGAAGGCTGTTAAACAATACAGGAGCGGCAAAAAAGGCGTTGCGGGATATT
TCGTTGGTATCGTGATGAAAGGAACGAAAGGAAAAGCCAATCCAGCTCTTGTAAATGAGATTGTCAGGAGGTTGCTGGAA
GAATGA

Upstream 100 bases:

>100_bases
GAAGATGTTCGGATGTTAAACACAGCAAGAATTCTGGAGAAGCTTTCTCCATCTTATGATGAAAATGGTCTGGCGAGAAT
AGCTGAAAGGTGGTATTGAC

Downstream 100 bases:

>100_bases
AAAAAACATTTATGATAGGCCTATTTATCATTTTTTCTGTTTTGATATATGCTGCACCCACAGTACGATTTACACTTCAG
GATGCTTTTTGGTCAACTGA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MYKAVIGLEIHAQLSTVTKAFCNCSADVFELEPNTAICPVCTGQPGSLPVLNEKVVEYALKAALAFNCEINLFSSFDRKN
YFYPDLPKGYQITQYFHPIAEHGYLMIGTGESRKKVRIRRIHIEEDAGKMLHQGADSISGATESFVDLNRCGVPLIEIVT
EPDLNSPKEAREFMELLRDTLRYIEVCSGDMEKGALRCDANISVYDTETGKSSERVEVKNINSFKFVEKALEFEFERIKA
ALENGENIVRETRSWNFSARETTSMRSKEEENDYRYFPEPDLPELKLTQEYVDKVKKELPELPWEKQSRFVQEYGIPEYD
ASVLCSDKELANFFEDVARATGKPKETSNWIMTEFLRELKQRNASVDQLGIKPEHFVDLFKLLDSGKISTKIAKELFSKM
VENKKMPSELVEELGLQQINDEKLIEELIRKAMEANPKAVKQYRSGKKGVAGYFVGIVMKGTKGKANPALVNEIVRRLLE
E

Sequences:

>Translated_481_residues
MYKAVIGLEIHAQLSTVTKAFCNCSADVFELEPNTAICPVCTGQPGSLPVLNEKVVEYALKAALAFNCEINLFSSFDRKN
YFYPDLPKGYQITQYFHPIAEHGYLMIGTGESRKKVRIRRIHIEEDAGKMLHQGADSISGATESFVDLNRCGVPLIEIVT
EPDLNSPKEAREFMELLRDTLRYIEVCSGDMEKGALRCDANISVYDTETGKSSERVEVKNINSFKFVEKALEFEFERIKA
ALENGENIVRETRSWNFSARETTSMRSKEEENDYRYFPEPDLPELKLTQEYVDKVKKELPELPWEKQSRFVQEYGIPEYD
ASVLCSDKELANFFEDVARATGKPKETSNWIMTEFLRELKQRNASVDQLGIKPEHFVDLFKLLDSGKISTKIAKELFSKM
VENKKMPSELVEELGLQQINDEKLIEELIRKAMEANPKAVKQYRSGKKGVAGYFVGIVMKGTKGKANPALVNEIVRRLLE
E
>Mature_481_residues
MYKAVIGLEIHAQLSTVTKAFCNCSADVFELEPNTAICPVCTGQPGSLPVLNEKVVEYALKAALAFNCEINLFSSFDRKN
YFYPDLPKGYQITQYFHPIAEHGYLMIGTGESRKKVRIRRIHIEEDAGKMLHQGADSISGATESFVDLNRCGVPLIEIVT
EPDLNSPKEAREFMELLRDTLRYIEVCSGDMEKGALRCDANISVYDTETGKSSERVEVKNINSFKFVEKALEFEFERIKA
ALENGENIVRETRSWNFSARETTSMRSKEEENDYRYFPEPDLPELKLTQEYVDKVKKELPELPWEKQSRFVQEYGIPEYD
ASVLCSDKELANFFEDVARATGKPKETSNWIMTEFLRELKQRNASVDQLGIKPEHFVDLFKLLDSGKISTKIAKELFSKM
VENKKMPSELVEELGLQQINDEKLIEELIRKAMEANPKAVKQYRSGKKGVAGYFVGIVMKGTKGKANPALVNEIVRRLLE
E

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=501, Percent_Identity=37.7245508982036, Blast_Score=320, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI115534094, Length=464, Percent_Identity=34.051724137931, Blast_Score=220, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6319391, Length=508, Percent_Identity=30.7086614173228, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=35.6136820925553, Blast_Score=284, Evalue=9e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54817; Mature: 54817

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKAVIGLEIHAQLSTVTKAFCNCSADVFELEPNTAICPVCTGQPGSLPVLNEKVVEYAL
CCCEEEEEEHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHH
KAALAFNCEINLFSSFDRKNYFYPDLPKGYQITQYFHPIAEHGYLMIGTGESRKKVRIRR
HHHHHCCCEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCEEEEE
IHIEEDAGKMLHQGADSISGATESFVDLNRCGVPLIEIVTEPDLNSPKEAREFMELLRDT
EEECHHHHHHHHCCCHHHCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
LRYIEVCSGDMEKGALRCDANISVYDTETGKSSERVEVKNINSFKFVEKALEFEFERIKA
HHHHHHHCCCHHCCCEEECCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
ALENGENIVRETRSWNFSARETTSMRSKEEENDYRYFPEPDLPELKLTQEYVDKVKKELP
HHHCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
ELPWEKQSRFVQEYGIPEYDASVLCSDKELANFFEDVARATGKPKETSNWIMTEFLRELK
CCCCHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
QRNASVDQLGIKPEHFVDLFKLLDSGKISTKIAKELFSKMVENKKMPSELVEELGLQQIN
HCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHCCC
DEKLIEELIRKAMEANPKAVKQYRSGKKGVAGYFVGIVMKGTKGKANPALVNEIVRRLLE
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHEEEEEEEECCCCCCCHHHHHHHHHHHHC
E
C
>Mature Secondary Structure
MYKAVIGLEIHAQLSTVTKAFCNCSADVFELEPNTAICPVCTGQPGSLPVLNEKVVEYAL
CCCEEEEEEHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHH
KAALAFNCEINLFSSFDRKNYFYPDLPKGYQITQYFHPIAEHGYLMIGTGESRKKVRIRR
HHHHHCCCEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCEEEEE
IHIEEDAGKMLHQGADSISGATESFVDLNRCGVPLIEIVTEPDLNSPKEAREFMELLRDT
EEECHHHHHHHHCCCHHHCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
LRYIEVCSGDMEKGALRCDANISVYDTETGKSSERVEVKNINSFKFVEKALEFEFERIKA
HHHHHHHCCCHHCCCEEECCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
ALENGENIVRETRSWNFSARETTSMRSKEEENDYRYFPEPDLPELKLTQEYVDKVKKELP
HHHCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
ELPWEKQSRFVQEYGIPEYDASVLCSDKELANFFEDVARATGKPKETSNWIMTEFLRELK
CCCCHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
QRNASVDQLGIKPEHFVDLFKLLDSGKISTKIAKELFSKMVENKKMPSELVEELGLQQIN
HCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHCCC
DEKLIEELIRKAMEANPKAVKQYRSGKKGVAGYFVGIVMKGTKGKANPALVNEIVRRLLE
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHEEEEEEEECCCCCCCHHHHHHHHHHHHC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]