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The map label for this gene is gatA [H]

Identifier: 239616733

GI number: 239616733

Start: 346350

End: 347777

Strand: Reverse

Name: gatA [H]

Synonym: Kole_0326

Alternate gene names: 239616733

Gene position: 347777-346350 (Counterclockwise)

Preceding gene: 239616737

Following gene: 239616732

Centisome position: 15.11

GC content: 42.51

Gene sequence:

>1428_bases
TTGCCAAATAAATTTCTTCGAATGAAAATAAAAGAACTCGTAAATCTAAAGGACAAAGAAAGCATTTTTCGTTTCTTTAA
CGATAGGATTAACACCTACAATGATGAACTGAATGCTTTTACAGAGATAATTCCCGATCCTGATAGAACCTCAGGAGAAG
GGCCACTTAATGGGATTCCCTACGCCTTAAAGGATAACATTCTTGCCAGAGGAACTAAAACCACATGTGGCAGTAAGATT
CTATCAAATTATAAATCAGTCTACGATGCCACGGTAACAAAGCGTCTTAGGGACTCTGGAGCAATATTGATGGGAAAGAC
AAATATGGACGAATTCGCTATGGGGTCTTCAACGGAATTCTCAGCTTTTGGACCTTCAAAGAATCCATGGGATGCAAATA
GAGTTCCGGGTGGAAGCAGTGGTGGAAGTGCTGCCGCAGTAGCTGCAGGTTTGGTCCCCTTTGCTCTGGGAAGTGATACT
GGAGGTTCCATCAGGCAACCTGCCGCTTTTTGCGGTGTTGTGGGTTATAAGCCTAGCTATGGATTGGTTTCGCGTTATGG
ACTGGTAGCTTTTGCCTCATCTCTTGATCAAATCGGTCCAATAACTCGATGTGTGGATGATGCCGTTGAAGTAGCAAAAG
TAATAATCGGATTCGATCCAGATGATTCCACAACGATCGAGCATCCTTTGAACTTTGAAAACGAAGAGCCGAAAGACTTA
ACCGGCCTAAAATTCGCCCTTCCTGGGGAAATGATTGAATATCCCGGGCTTCAAGAAAGTGTAAAAGAGGTATTTATTAA
AATGGTGGAATCTATCGAAAAAGCTGGCGGTATCGTGGAGAAGGTCTCCATTCCATCACTCAAATATGTTGTTGCCACTT
ATTATCTTATAGCACCAGGAGAAGCGAGTTCGAACCTTTCTCGTTATGATGGTATTAGATACGGACAGCGTAAAGATGCG
GATAGATATGAAGAAGTTGTTAATCAAAGCAGGGATTTTGGTTTTGGCGACGAAGTGAAGAGAAGGATACTTCTCGGGAC
CTTTACCTTAAGCGCAGCCTATTATGACGCGTACTACCGTAAAGCCCTGAGGGTTAGAAAGCTTATTTCAAAAGAAATCA
ATGAGATTCTCTCGAAATACGATTTTGTGATCAACCCAACGAGCCCTATTGTAGCTCCTAAGATAGGTGAAATAAGCGAT
CCTTTGACTTATTATCTAATGGATATTTACACGATTCCGGCCAATCTTGCTGGTCTTCCCGCAATATCACTTCCAGCAGG
CGCTTATGAAGGGTTACCCGTTGGTTTTCAGATGATGGCGAGACGATTCGAAGATGTTCGGATGTTAAACACAGCAAGAA
TTCTGGAGAAGCTTTCTCCATCTTATGATGAAAATGGTCTGGCGAGAATAGCTGAAAGGTGGTATTGA

Upstream 100 bases:

>100_bases
CCTGTTAGGTTATGATTCAGAGTAAAATTACCCGATAGCTTTTACAAGCTTTTCAATATATGATGCTACAATACTTTTGT
AAAAATGGGAGGTGGGTCAG

Downstream 100 bases:

>100_bases
CATGTATAAAGCAGTTATAGGTTTGGAGATACATGCCCAGCTCTCAACAGTGACAAAGGCCTTTTGTAATTGTAGCGCCG
ATGTCTTCGAATTGGAACCT

Product: glutamyl-tRNA(Gln) amidotransferase, A subunit

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MPNKFLRMKIKELVNLKDKESIFRFFNDRINTYNDELNAFTEIIPDPDRTSGEGPLNGIPYALKDNILARGTKTTCGSKI
LSNYKSVYDATVTKRLRDSGAILMGKTNMDEFAMGSSTEFSAFGPSKNPWDANRVPGGSSGGSAAAVAAGLVPFALGSDT
GGSIRQPAAFCGVVGYKPSYGLVSRYGLVAFASSLDQIGPITRCVDDAVEVAKVIIGFDPDDSTTIEHPLNFENEEPKDL
TGLKFALPGEMIEYPGLQESVKEVFIKMVESIEKAGGIVEKVSIPSLKYVVATYYLIAPGEASSNLSRYDGIRYGQRKDA
DRYEEVVNQSRDFGFGDEVKRRILLGTFTLSAAYYDAYYRKALRVRKLISKEINEILSKYDFVINPTSPIVAPKIGEISD
PLTYYLMDIYTIPANLAGLPAISLPAGAYEGLPVGFQMMARRFEDVRMLNTARILEKLSPSYDENGLARIAERWY

Sequences:

>Translated_475_residues
MPNKFLRMKIKELVNLKDKESIFRFFNDRINTYNDELNAFTEIIPDPDRTSGEGPLNGIPYALKDNILARGTKTTCGSKI
LSNYKSVYDATVTKRLRDSGAILMGKTNMDEFAMGSSTEFSAFGPSKNPWDANRVPGGSSGGSAAAVAAGLVPFALGSDT
GGSIRQPAAFCGVVGYKPSYGLVSRYGLVAFASSLDQIGPITRCVDDAVEVAKVIIGFDPDDSTTIEHPLNFENEEPKDL
TGLKFALPGEMIEYPGLQESVKEVFIKMVESIEKAGGIVEKVSIPSLKYVVATYYLIAPGEASSNLSRYDGIRYGQRKDA
DRYEEVVNQSRDFGFGDEVKRRILLGTFTLSAAYYDAYYRKALRVRKLISKEINEILSKYDFVINPTSPIVAPKIGEISD
PLTYYLMDIYTIPANLAGLPAISLPAGAYEGLPVGFQMMARRFEDVRMLNTARILEKLSPSYDENGLARIAERWY
>Mature_474_residues
PNKFLRMKIKELVNLKDKESIFRFFNDRINTYNDELNAFTEIIPDPDRTSGEGPLNGIPYALKDNILARGTKTTCGSKIL
SNYKSVYDATVTKRLRDSGAILMGKTNMDEFAMGSSTEFSAFGPSKNPWDANRVPGGSSGGSAAAVAAGLVPFALGSDTG
GSIRQPAAFCGVVGYKPSYGLVSRYGLVAFASSLDQIGPITRCVDDAVEVAKVIIGFDPDDSTTIEHPLNFENEEPKDLT
GLKFALPGEMIEYPGLQESVKEVFIKMVESIEKAGGIVEKVSIPSLKYVVATYYLIAPGEASSNLSRYDGIRYGQRKDAD
RYEEVVNQSRDFGFGDEVKRRILLGTFTLSAAYYDAYYRKALRVRKLISKEINEILSKYDFVINPTSPIVAPKIGEISDP
LTYYLMDIYTIPANLAGLPAISLPAGAYEGLPVGFQMMARRFEDVRMLNTARILEKLSPSYDENGLARIAERWY

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=432, Percent_Identity=40.7407407407407, Blast_Score=295, Evalue=6e-80,
Organism=Homo sapiens, GI195972892, Length=418, Percent_Identity=28.7081339712919, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI166795287, Length=195, Percent_Identity=29.7435897435897, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=419, Percent_Identity=37.708830548926, Blast_Score=278, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17537465, Length=435, Percent_Identity=25.9770114942529, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17556264, Length=198, Percent_Identity=34.8484848484849, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=164, Percent_Identity=34.1463414634146, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=172, Percent_Identity=31.3953488372093, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=158, Percent_Identity=38.6075949367089, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=158, Percent_Identity=38.6075949367089, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=459, Percent_Identity=35.9477124183007, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6319685, Length=415, Percent_Identity=30.1204819277108, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320448, Length=140, Percent_Identity=34.2857142857143, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24648113, Length=452, Percent_Identity=39.6017699115044, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24652985, Length=422, Percent_Identity=29.1469194312796, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19922090, Length=422, Percent_Identity=29.1469194312796, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24652981, Length=422, Percent_Identity=29.1469194312796, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24652983, Length=422, Percent_Identity=29.1469194312796, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24644968, Length=425, Percent_Identity=27.0588235294118, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078093, Length=446, Percent_Identity=28.9237668161435, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21356731, Length=465, Percent_Identity=25.1612903225806, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648435, Length=415, Percent_Identity=24.8192771084337, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648437, Length=415, Percent_Identity=24.8192771084337, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45550774, Length=415, Percent_Identity=24.8192771084337, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648441, Length=415, Percent_Identity=25.0602409638554, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648439, Length=415, Percent_Identity=25.0602409638554, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52342; Mature: 52211

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNKFLRMKIKELVNLKDKESIFRFFNDRINTYNDELNAFTEIIPDPDRTSGEGPLNGIP
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
YALKDNILARGTKTTCGSKILSNYKSVYDATVTKRLRDSGAILMGKTNMDEFAMGSSTEF
HHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCCCC
SAFGPSKNPWDANRVPGGSSGGSAAAVAAGLVPFALGSDTGGSIRQPAAFCGVVGYKPSY
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCC
GLVSRYGLVAFASSLDQIGPITRCVDDAVEVAKVIIGFDPDDSTTIEHPLNFENEEPKDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
TGLKFALPGEMIEYPGLQESVKEVFIKMVESIEKAGGIVEKVSIPSLKYVVATYYLIAPG
CCEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHEECCC
EASSNLSRYDGIRYGQRKDADRYEEVVNQSRDFGFGDEVKRRILLGTFTLSAAYYDAYYR
CCCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KALRVRKLISKEINEILSKYDFVINPTSPIVAPKIGEISDPLTYYLMDIYTIPANLAGLP
HHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
AISLPAGAYEGLPVGFQMMARRFEDVRMLNTARILEKLSPSYDENGLARIAERWY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
PNKFLRMKIKELVNLKDKESIFRFFNDRINTYNDELNAFTEIIPDPDRTSGEGPLNGIP
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
YALKDNILARGTKTTCGSKILSNYKSVYDATVTKRLRDSGAILMGKTNMDEFAMGSSTEF
HHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCCCC
SAFGPSKNPWDANRVPGGSSGGSAAAVAAGLVPFALGSDTGGSIRQPAAFCGVVGYKPSY
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCC
GLVSRYGLVAFASSLDQIGPITRCVDDAVEVAKVIIGFDPDDSTTIEHPLNFENEEPKDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
TGLKFALPGEMIEYPGLQESVKEVFIKMVESIEKAGGIVEKVSIPSLKYVVATYYLIAPG
CCEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHEECCC
EASSNLSRYDGIRYGQRKDADRYEEVVNQSRDFGFGDEVKRRILLGTFTLSAAYYDAYYR
CCCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KALRVRKLISKEINEILSKYDFVINPTSPIVAPKIGEISDPLTYYLMDIYTIPANLAGLP
HHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
AISLPAGAYEGLPVGFQMMARRFEDVRMLNTARILEKLSPSYDENGLARIAERWY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]