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The map label for this gene is engD [H]

Identifier: 239616636

GI number: 239616636

Start: 257409

End: 258488

Strand: Reverse

Name: engD [H]

Synonym: Kole_0229

Alternate gene names: 239616636

Gene position: 258488-257409 (Counterclockwise)

Preceding gene: 239616639

Following gene: 239616635

Centisome position: 11.23

GC content: 45.65

Gene sequence:

>1080_bases
ATGGAAATCGGTATTTTCGGTTTACCTTTGAGTGGTAAAACGACGATTTTTTCTCTTCTGACAGGAGTGGATACCTCCGG
TTCACATAGAACAGAATCCCACACAGGTGTTGCAAAAATATACGATGAACGGGTTGCAAAGCTTTCAGAGATATTCAAAC
CAAAGAAGACAACATACGCTACCCTGAGCTTCATCGATATCCCCAGCTTCGATCCATCCGCAAACCGCAAGGAGAAGAAT
CGTATCTTCCAGTTCATACAGAATGCAGATGCTTTGCTGGCTGTGGTTCGAACCTTTAGAGATCCCACCGTTCCATGGCC
TGTTGGAGCTGAAACTCCAAAACAACAGCTTGATGCGATCATCACGGAGCTTTTGCTTAGGGATATGGAAGTGGTAGAGA
ACAAGATCGAACGGCTCAACGAAAGCTTAAAGAAAAAGAAATTGACAAAGGAAGAAGAAAAGGAGCTCGAGGTTCTGGGA
GAGATAAAAAACGCACTCGAGGAAGAGAAATTTGTTTCACAACTGGGTCTGGAAGCGGATATTCTCAAGCTTCTTGGCTC
GCTTTCTCTATTCACTGCCAAGCCGCTGATCATAGTGGCCAACACCGATGATGAACAGTTCACAGAAGGAAACTATGACG
GCAAAGAGGATATAAAAGCCACCTGCGAAGAGAACGGGTTTGCCTACATGGAACTCTCCGGGAAGATAGAGATGGAAATC
AACGAACTTGAAGATGAGGAAAAAGAAGCCTTCATGGAAGAGCTTGGTATAAAGGAAAGCGGGATACAAAGGCTTTCAAG
GGTTGTTTACGAGCATATCGGCTTGATGTCCTTTTTGACTGTTGGTGAGGATGAAGTCCGTGCCTGGACCATAAAGAAAG
GATCAACCGCCAGGGAAGCTGCGGGGAAGATACACACGGATCTTGCCAAAAACTTCATCCGTGCGGAGGTCATTCCCTAC
CAGACCTTCATAGAGATTGGAGATATGCACGAAGCCAAAAATAAGGGGCTTGTTAAACTCGTAGGAAGGGATGAGATCAT
ACATGACGGAGACATCGTACACATCAGGGCAAACGCGTGA

Upstream 100 bases:

>100_bases
CACTTCAGCAACGTAATCGCTCCCGTCTTTTGTAAAATCGCCAATTCTGGTAATATATAAGTGTATGTCTATTCGGTACT
AAAACGAGGGAGGCAATTTT

Downstream 100 bases:

>100_bases
GCTCAAAATCTGCGATACGCACGCCCATCTGAATTTTCCCCATTTCAACAGAGACCGGAAACGAATCCTTGAAGAGATAG
ATTCAGGAAAGATTGTTTTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MEIGIFGLPLSGKTTIFSLLTGVDTSGSHRTESHTGVAKIYDERVAKLSEIFKPKKTTYATLSFIDIPSFDPSANRKEKN
RIFQFIQNADALLAVVRTFRDPTVPWPVGAETPKQQLDAIITELLLRDMEVVENKIERLNESLKKKKLTKEEEKELEVLG
EIKNALEEEKFVSQLGLEADILKLLGSLSLFTAKPLIIVANTDDEQFTEGNYDGKEDIKATCEENGFAYMELSGKIEMEI
NELEDEEKEAFMEELGIKESGIQRLSRVVYEHIGLMSFLTVGEDEVRAWTIKKGSTAREAAGKIHTDLAKNFIRAEVIPY
QTFIEIGDMHEAKNKGLVKLVGRDEIIHDGDIVHIRANA

Sequences:

>Translated_359_residues
MEIGIFGLPLSGKTTIFSLLTGVDTSGSHRTESHTGVAKIYDERVAKLSEIFKPKKTTYATLSFIDIPSFDPSANRKEKN
RIFQFIQNADALLAVVRTFRDPTVPWPVGAETPKQQLDAIITELLLRDMEVVENKIERLNESLKKKKLTKEEEKELEVLG
EIKNALEEEKFVSQLGLEADILKLLGSLSLFTAKPLIIVANTDDEQFTEGNYDGKEDIKATCEENGFAYMELSGKIEMEI
NELEDEEKEAFMEELGIKESGIQRLSRVVYEHIGLMSFLTVGEDEVRAWTIKKGSTAREAAGKIHTDLAKNFIRAEVIPY
QTFIEIGDMHEAKNKGLVKLVGRDEIIHDGDIVHIRANA
>Mature_359_residues
MEIGIFGLPLSGKTTIFSLLTGVDTSGSHRTESHTGVAKIYDERVAKLSEIFKPKKTTYATLSFIDIPSFDPSANRKEKN
RIFQFIQNADALLAVVRTFRDPTVPWPVGAETPKQQLDAIITELLLRDMEVVENKIERLNESLKKKKLTKEEEKELEVLG
EIKNALEEEKFVSQLGLEADILKLLGSLSLFTAKPLIIVANTDDEQFTEGNYDGKEDIKATCEENGFAYMELSGKIEMEI
NELEDEEKEAFMEELGIKESGIQRLSRVVYEHIGLMSFLTVGEDEVRAWTIKKGSTAREAAGKIHTDLAKNFIRAEVIPY
QTFIEIGDMHEAKNKGLVKLVGRDEIIHDGDIVHIRANA

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=368, Percent_Identity=28.5326086956522, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI58761502, Length=179, Percent_Identity=33.5195530726257, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1787454, Length=364, Percent_Identity=34.3406593406593, Blast_Score=207, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17509631, Length=367, Percent_Identity=29.1553133514986, Blast_Score=164, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6319499, Length=376, Percent_Identity=27.6595744680851, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6321773, Length=379, Percent_Identity=26.9129287598945, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24640873, Length=370, Percent_Identity=30.8108108108108, Blast_Score=148, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24640877, Length=370, Percent_Identity=30.8108108108108, Blast_Score=148, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24640875, Length=370, Percent_Identity=30.8108108108108, Blast_Score=148, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24640879, Length=327, Percent_Identity=31.4984709480122, Blast_Score=134, Evalue=6e-32,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40346; Mature: 40346

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIGIFGLPLSGKTTIFSLLTGVDTSGSHRTESHTGVAKIYDERVAKLSEIFKPKKTTYA
CCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
TLSFIDIPSFDPSANRKEKNRIFQFIQNADALLAVVRTFRDPTVPWPVGAETPKQQLDAI
EEEEEECCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
ITELLLRDMEVVENKIERLNESLKKKKLTKEEEKELEVLGEIKNALEEEKFVSQLGLEAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ILKLLGSLSLFTAKPLIIVANTDDEQFTEGNYDGKEDIKATCEENGFAYMELSGKIEMEI
HHHHHHHHHHHCCCCEEEEECCCCHHHCCCCCCCHHHHHHHHCCCCEEEEEECCEEEEEH
NELEDEEKEAFMEELGIKESGIQRLSRVVYEHIGLMSFLTVGEDEVRAWTIKKGSTAREA
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCHHHH
AGKIHTDLAKNFIRAEVIPYQTFIEIGDMHEAKNKGLVKLVGRDEIIHDGDIVHIRANA
HHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHCCCCEEEEECCCCEEECCCEEEEEECC
>Mature Secondary Structure
MEIGIFGLPLSGKTTIFSLLTGVDTSGSHRTESHTGVAKIYDERVAKLSEIFKPKKTTYA
CCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
TLSFIDIPSFDPSANRKEKNRIFQFIQNADALLAVVRTFRDPTVPWPVGAETPKQQLDAI
EEEEEECCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
ITELLLRDMEVVENKIERLNESLKKKKLTKEEEKELEVLGEIKNALEEEKFVSQLGLEAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
ILKLLGSLSLFTAKPLIIVANTDDEQFTEGNYDGKEDIKATCEENGFAYMELSGKIEMEI
HHHHHHHHHHHCCCCEEEEECCCCHHHCCCCCCCHHHHHHHHCCCCEEEEEECCEEEEEH
NELEDEEKEAFMEELGIKESGIQRLSRVVYEHIGLMSFLTVGEDEVRAWTIKKGSTAREA
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCHHHH
AGKIHTDLAKNFIRAEVIPYQTFIEIGDMHEAKNKGLVKLVGRDEIIHDGDIVHIRANA
HHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHCCCCEEEEECCCCEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]