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The map label for this gene is ruvB

Identifier: 239616615

GI number: 239616615

Start: 235645

End: 236673

Strand: Reverse

Name: ruvB

Synonym: Kole_0208

Alternate gene names: 239616615

Gene position: 236673-235645 (Counterclockwise)

Preceding gene: 239616616

Following gene: 239616614

Centisome position: 10.28

GC content: 51.41

Gene sequence:

>1029_bases
ATGGAAGAACGATTTCTTACCCCAAAAGATATAGGTGAAGAACAGTTAACGCGGACATTAAGGCCTCAAAGGCTGGATGA
ATATATAGGCCAAAGGAACGTCAAACAGAGGCTGAAGATTTCCATAGAGGCAGCGAAAGTCAGGAACGAAGCCCTGGATC
ATGTTTTGCTTGCGGGGCCACCCGGGCTCGGTAAAACTACCCTAGCCCACATCATCGCCAACGAGATGGGAACGAACATC
TACGTTACCAGCGGGCCCGTCATAGAAAAGCAGGGCGATCTCGCTGCCATACTTACCAGCCTTGAGGAAGGCGATGTGTT
GTTTATCGATGAGATACATCGCCTTGGCCGTGCTATCGAAGAGATCCTTTACTCTGCGATGGAAGATTTTAAGCTGGATA
TTATGATCGGGAAAGGCCCCAGTGCGCGTTCCATAAGGCTCGATCTCGCTCCTTTCACGCTCGTAGGGGCAACAACGCGT
TCGGGTTTGATCGGCGCACCGCTCAGGAACAGGTTCGGCATGGTTCTCGAACTCGAGTTTTATACTCCGGATGAGCTGAA
GCAGATAATAAAGAGATCGGCGCGACTGCTTGAAGTGAAGATAGATGACGAAGCTGCCGAGCTTCTCGCTTCCCGGTCCA
GAGGCACGCCCAGAATCGCCAACAGGCTTTTGAGGCGTGTCAGGGATCTGGCTACCGTTGATGGTACGGGGAAGATTCTC
TCTAAGACCGTAGAGGATGCCATGAAGATCATGGGGATCGACGCTGAGGGCCTCGACGATATGGACAGGAAGATCTTGAG
GGTTCTTATTGAGAATTACGAAGGCGGACCTGCTGGTCTCAAAGCCATCGCGGCCTCGGTGGGTATCGAAGCTGATACGA
TAAGCGAGGTATATGAACCGTATCTGCTGCAAAGCGGTTTCATAGTTCGGACGAACAGAGGCCGTATGGCAACGAAAAAA
GCTTACCGCCATCTCGGTATTGCGAGATTTAACGGGATCGGTCCATTATGGGATAGTACAGGGGACTAA

Upstream 100 bases:

>100_bases
GATCGAGAGGGTCGTGGCCTGGATAGCCGGACTGGAGCACGTCAGGGAAGCGATTCCCTTTGCAAGAACGCTTTACAGAA
TTCATCCGTGAGGAGATAAA

Downstream 100 bases:

>100_bases
GGAGGAGGGAAAATGTTCGTACTTGCGAATCTTGTTTATGCTGTTGCTCTTGTGTTGAGGATTCTCGTGGAGTTTGAGAT
CTTCGCGGTTATCGCTTCAG

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MEERFLTPKDIGEEQLTRTLRPQRLDEYIGQRNVKQRLKISIEAAKVRNEALDHVLLAGPPGLGKTTLAHIIANEMGTNI
YVTSGPVIEKQGDLAAILTSLEEGDVLFIDEIHRLGRAIEEILYSAMEDFKLDIMIGKGPSARSIRLDLAPFTLVGATTR
SGLIGAPLRNRFGMVLELEFYTPDELKQIIKRSARLLEVKIDDEAAELLASRSRGTPRIANRLLRRVRDLATVDGTGKIL
SKTVEDAMKIMGIDAEGLDDMDRKILRVLIENYEGGPAGLKAIAASVGIEADTISEVYEPYLLQSGFIVRTNRGRMATKK
AYRHLGIARFNGIGPLWDSTGD

Sequences:

>Translated_342_residues
MEERFLTPKDIGEEQLTRTLRPQRLDEYIGQRNVKQRLKISIEAAKVRNEALDHVLLAGPPGLGKTTLAHIIANEMGTNI
YVTSGPVIEKQGDLAAILTSLEEGDVLFIDEIHRLGRAIEEILYSAMEDFKLDIMIGKGPSARSIRLDLAPFTLVGATTR
SGLIGAPLRNRFGMVLELEFYTPDELKQIIKRSARLLEVKIDDEAAELLASRSRGTPRIANRLLRRVRDLATVDGTGKIL
SKTVEDAMKIMGIDAEGLDDMDRKILRVLIENYEGGPAGLKAIAASVGIEADTISEVYEPYLLQSGFIVRTNRGRMATKK
AYRHLGIARFNGIGPLWDSTGD
>Mature_342_residues
MEERFLTPKDIGEEQLTRTLRPQRLDEYIGQRNVKQRLKISIEAAKVRNEALDHVLLAGPPGLGKTTLAHIIANEMGTNI
YVTSGPVIEKQGDLAAILTSLEEGDVLFIDEIHRLGRAIEEILYSAMEDFKLDIMIGKGPSARSIRLDLAPFTLVGATTR
SGLIGAPLRNRFGMVLELEFYTPDELKQIIKRSARLLEVKIDDEAAELLASRSRGTPRIANRLLRRVRDLATVDGTGKIL
SKTVEDAMKIMGIDAEGLDDMDRKILRVLIENYEGGPAGLKAIAASVGIEADTISEVYEPYLLQSGFIVRTNRGRMATKK
AYRHLGIARFNGIGPLWDSTGD

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=320, Percent_Identity=57.1875, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_KOSOT (C5CIU4)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002939937.1
- GeneID:   7968360
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_0208
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37819; Mature: 37819

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERFLTPKDIGEEQLTRTLRPQRLDEYIGQRNVKQRLKISIEAAKVRNEALDHVLLAGP
CCCCCCCHHHCCHHHHHHHCCHHHHHHHHHHHCHHHHHHEEHHHHHHHHHHHCEEEEECC
PGLGKTTLAHIIANEMGTNIYVTSGPVIEKQGDLAAILTSLEEGDVLFIDEIHRLGRAIE
CCCCHHHHHHHHHHHHCCEEEEECCCCEECCCCHHHHHHHCCCCCEEEHHHHHHHHHHHH
EILYSAMEDFKLDIMIGKGPSARSIRLDLAPFTLVGATTRSGLIGAPLRNRFGMVLELEF
HHHHHHHHCCEEEEEEECCCCCCEEEEEECCEEEEECCCCCCCCCCCHHHCCCEEEEEEE
YTPDELKQIIKRSARLLEVKIDDEAAELLASRSRGTPRIANRLLRRVRDLATVDGTGKIL
CCHHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
SKTVEDAMKIMGIDAEGLDDMDRKILRVLIENYEGGPAGLKAIAASVGIEADTISEVYEP
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
YLLQSGFIVRTNRGRMATKKAYRHLGIARFNGIGPLWDSTGD
HHHCCCEEEEECCCCHHHHHHHHHHCHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MEERFLTPKDIGEEQLTRTLRPQRLDEYIGQRNVKQRLKISIEAAKVRNEALDHVLLAGP
CCCCCCCHHHCCHHHHHHHCCHHHHHHHHHHHCHHHHHHEEHHHHHHHHHHHCEEEEECC
PGLGKTTLAHIIANEMGTNIYVTSGPVIEKQGDLAAILTSLEEGDVLFIDEIHRLGRAIE
CCCCHHHHHHHHHHHHCCEEEEECCCCEECCCCHHHHHHHCCCCCEEEHHHHHHHHHHHH
EILYSAMEDFKLDIMIGKGPSARSIRLDLAPFTLVGATTRSGLIGAPLRNRFGMVLELEF
HHHHHHHHCCEEEEEEECCCCCCEEEEEECCEEEEECCCCCCCCCCCHHHCCCEEEEEEE
YTPDELKQIIKRSARLLEVKIDDEAAELLASRSRGTPRIANRLLRRVRDLATVDGTGKIL
CCHHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
SKTVEDAMKIMGIDAEGLDDMDRKILRVLIENYEGGPAGLKAIAASVGIEADTISEVYEP
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
YLLQSGFIVRTNRGRMATKKAYRHLGIARFNGIGPLWDSTGD
HHHCCCEEEEECCCCHHHHHHHHHHCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA