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The map label for this gene is asnS [H]

Identifier: 239616616

GI number: 239616616

Start: 236683

End: 237981

Strand: Reverse

Name: asnS [H]

Synonym: Kole_0209

Alternate gene names: 239616616

Gene position: 237981-236683 (Counterclockwise)

Preceding gene: 239616617

Following gene: 239616615

Centisome position: 10.34

GC content: 49.96

Gene sequence:

>1299_bases
ATGAACTGGGTTTATATACGTGATCTGAAAGACCACATCGGCGAAAGGGTCGAGATACGCGGCTGGCTCAGGAGAAAACG
TTCGAGTGGTAAGATACACTTTCTCGCTTTGAGAGACGGTTCCGGTTTCGTTCAGGGAATTGTCGAACGCTCTTCGGTTT
CCGAGGAACTCTTCAAACTGGCGAAAAAGCTGAAGATGGAGTCGAGCATAATCGCTCGCGGAATCGTCAGGGAAGATCCC
AGGGCTCCCGAAGGTGTTGAACTGCTCATTGATGAAATAGAAGTGGTTCACCAGCCTGAAAAGGATTTCCCGATAAACAA
GCCCGACCACGGGATCGATTATCTCATGGACCATCGACATCTCTGGTTGAGATCAAAGAGACAATTCCATATCCTGCACA
TCAGACATGAGATAATCAGGGCCGTGAGGGATTTCTATAACGAGCGCGGTTTTGTTCTTATCGACACACCAATATTCACC
GGGTCTATCGGTGAAAGTGCGGGAAACTGTTTCGAAATCGATTATTTTGACTACGGCAAGGCGTACCTCGCCCAGACAGG
GCAGCTTTACCTTGAGGCCGCCTGTATGGCTCTTGGGAAGGTTTATAACCTTGGACCGACCTTTAGGGCGGAGAAGTCAA
AAACGAGAAGGCATCTTATAGAATTCTGGATGAACGAAGCGGAGGTTGCCTACTACCAGCATGAGGACAACATGAAACTT
CAGGAAGAACTCGTTTCGTACGTTGTCCAGAAGACCATCGAACGTGCTGCTAATCATCTGCACGCCATTGGAAGGGATAT
CTCTAAACTCGAGAAGATCGAAGCGCCGTTCCCCAGAATCACCTACACCGACGCGATAAAGCTCCTTCAGAAGAAGGGAT
TCAACGTTAATTGGGGAGACGACATCGGCGGTGACGAGGAAACGGCAATAGCTGAGGAATTCGATAAGCCTGTTATAGTC
GAGCGCTACCCGAGAAAGATGAAGGCCTTTTACATGCAGCCCGATCCTGAGAATCCCGACGTGGTTCTCTGTGACGATAT
GCTGGCTCCGGAAGGCTACGGCGAGATAATCGGCGCTTCTGAGAGGATTTGGGATAAAGAACTGCTTATAGAGCGTATCA
AGGAGTTTGGCCTTGATGTGGAATCCTACAGCTGGTATCTCGATTTGCGTGAATACGGCTCTGTGCCGCACAGCGGATTC
GGCATGGGGATCGAGAGGGTCGTGGCCTGGATAGCCGGACTGGAGCACGTCAGGGAAGCGATTCCCTTTGCAAGAACGCT
TTACAGAATTCATCCGTGA

Upstream 100 bases:

>100_bases
AAGACAATCTTACATAAAAAATCCCCGGGAAATGTCCCGGGGTTTTTTGTTATAATCTAAGAAAGGAACGGGAAAAGAAC
GCGGATCTGGAGGTGTTTGA

Downstream 100 bases:

>100_bases
GGAGATAAAATGGAAGAACGATTTCTTACCCCAAAAGATATAGGTGAAGAACAGTTAACGCGGACATTAAGGCCTCAAAG
GCTGGATGAATATATAGGCC

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MNWVYIRDLKDHIGERVEIRGWLRRKRSSGKIHFLALRDGSGFVQGIVERSSVSEELFKLAKKLKMESSIIARGIVREDP
RAPEGVELLIDEIEVVHQPEKDFPINKPDHGIDYLMDHRHLWLRSKRQFHILHIRHEIIRAVRDFYNERGFVLIDTPIFT
GSIGESAGNCFEIDYFDYGKAYLAQTGQLYLEAACMALGKVYNLGPTFRAEKSKTRRHLIEFWMNEAEVAYYQHEDNMKL
QEELVSYVVQKTIERAANHLHAIGRDISKLEKIEAPFPRITYTDAIKLLQKKGFNVNWGDDIGGDEETAIAEEFDKPVIV
ERYPRKMKAFYMQPDPENPDVVLCDDMLAPEGYGEIIGASERIWDKELLIERIKEFGLDVESYSWYLDLREYGSVPHSGF
GMGIERVVAWIAGLEHVREAIPFARTLYRIHP

Sequences:

>Translated_432_residues
MNWVYIRDLKDHIGERVEIRGWLRRKRSSGKIHFLALRDGSGFVQGIVERSSVSEELFKLAKKLKMESSIIARGIVREDP
RAPEGVELLIDEIEVVHQPEKDFPINKPDHGIDYLMDHRHLWLRSKRQFHILHIRHEIIRAVRDFYNERGFVLIDTPIFT
GSIGESAGNCFEIDYFDYGKAYLAQTGQLYLEAACMALGKVYNLGPTFRAEKSKTRRHLIEFWMNEAEVAYYQHEDNMKL
QEELVSYVVQKTIERAANHLHAIGRDISKLEKIEAPFPRITYTDAIKLLQKKGFNVNWGDDIGGDEETAIAEEFDKPVIV
ERYPRKMKAFYMQPDPENPDVVLCDDMLAPEGYGEIIGASERIWDKELLIERIKEFGLDVESYSWYLDLREYGSVPHSGF
GMGIERVVAWIAGLEHVREAIPFARTLYRIHP
>Mature_432_residues
MNWVYIRDLKDHIGERVEIRGWLRRKRSSGKIHFLALRDGSGFVQGIVERSSVSEELFKLAKKLKMESSIIARGIVREDP
RAPEGVELLIDEIEVVHQPEKDFPINKPDHGIDYLMDHRHLWLRSKRQFHILHIRHEIIRAVRDFYNERGFVLIDTPIFT
GSIGESAGNCFEIDYFDYGKAYLAQTGQLYLEAACMALGKVYNLGPTFRAEKSKTRRHLIEFWMNEAEVAYYQHEDNMKL
QEELVSYVVQKTIERAANHLHAIGRDISKLEKIEAPFPRITYTDAIKLLQKKGFNVNWGDDIGGDEETAIAEEFDKPVIV
ERYPRKMKAFYMQPDPENPDVVLCDDMLAPEGYGEIIGASERIWDKELLIERIKEFGLDVESYSWYLDLREYGSVPHSGF
GMGIERVVAWIAGLEHVREAIPFARTLYRIHP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4758762, Length=445, Percent_Identity=33.2584269662921, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI226491502, Length=438, Percent_Identity=34.703196347032, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI45439306, Length=464, Percent_Identity=30.6034482758621, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1787161, Length=452, Percent_Identity=38.4955752212389, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1788173, Length=248, Percent_Identity=27.0161290322581, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71984122, Length=438, Percent_Identity=35.6164383561644, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17551876, Length=469, Percent_Identity=27.7185501066098, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17556372, Length=408, Percent_Identity=30.8823529411765, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI32566633, Length=304, Percent_Identity=26.6447368421053, Blast_Score=88, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=442, Percent_Identity=35.2941176470588, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6319872, Length=455, Percent_Identity=30.1098901098901, Blast_Score=189, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6323011, Length=469, Percent_Identity=28.5714285714286, Blast_Score=172, Evalue=9e-44,
Organism=Drosophila melanogaster, GI19921528, Length=432, Percent_Identity=34.0277777777778, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI19922416, Length=447, Percent_Identity=30.8724832214765, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI17136276, Length=463, Percent_Identity=29.1576673866091, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24584738, Length=290, Percent_Identity=25.5172413793103, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 50156; Mature: 50156

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWVYIRDLKDHIGERVEIRGWLRRKRSSGKIHFLALRDGSGFVQGIVERSSVSEELFKL
CCEEEEECHHHHCCCEEHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
AKKLKMESSIIARGIVREDPRAPEGVELLIDEIEVVHQPEKDFPINKPDHGIDYLMDHRH
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
LWLRSKRQFHILHIRHEIIRAVRDFYNERGFVLIDTPIFTGSIGESAGNCFEIDYFDYGK
HHHCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCCCCCEEEEECCCCCH
AYLAQTGQLYLEAACMALGKVYNLGPTFRAEKSKTRRHLIEFWMNEAEVAYYQHEDNMKL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
QEELVSYVVQKTIERAANHLHAIGRDISKLEKIEAPFPRITYTDAIKLLQKKGFNVNWGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCC
DIGGDEETAIAEEFDKPVIVERYPRKMKAFYMQPDPENPDVVLCDDMLAPEGYGEIIGAS
CCCCCCHHHHHHHCCCCEEHHHCCCHHHHEEECCCCCCCCEEEECCCCCCCCCHHHCCCC
ERIWDKELLIERIKEFGLDVESYSWYLDLREYGSVPHSGFGMGIERVVAWIAGLEHVREA
HHHHHHHHHHHHHHHHCCCCCHHEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IPFARTLYRIHP
HHHHHHHHHCCC
>Mature Secondary Structure
MNWVYIRDLKDHIGERVEIRGWLRRKRSSGKIHFLALRDGSGFVQGIVERSSVSEELFKL
CCEEEEECHHHHCCCEEHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
AKKLKMESSIIARGIVREDPRAPEGVELLIDEIEVVHQPEKDFPINKPDHGIDYLMDHRH
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
LWLRSKRQFHILHIRHEIIRAVRDFYNERGFVLIDTPIFTGSIGESAGNCFEIDYFDYGK
HHHCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCCCCCEEEEECCCCCH
AYLAQTGQLYLEAACMALGKVYNLGPTFRAEKSKTRRHLIEFWMNEAEVAYYQHEDNMKL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
QEELVSYVVQKTIERAANHLHAIGRDISKLEKIEAPFPRITYTDAIKLLQKKGFNVNWGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCC
DIGGDEETAIAEEFDKPVIVERYPRKMKAFYMQPDPENPDVVLCDDMLAPEGYGEIIGAS
CCCCCCHHHHHHHCCCCEEHHHCCCHHHHEEECCCCCCCCEEEECCCCCCCCCHHHCCCC
ERIWDKELLIERIKEFGLDVESYSWYLDLREYGSVPHSGFGMGIERVVAWIAGLEHVREA
HHHHHHHHHHHHHHHHCCCCCHHEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IPFARTLYRIHP
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA