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The map label for this gene is rfbB [C]

Identifier: 239616609

GI number: 239616609

Start: 227779

End: 228720

Strand: Reverse

Name: rfbB [C]

Synonym: Kole_0202

Alternate gene names: 239616609

Gene position: 228720-227779 (Counterclockwise)

Preceding gene: 239616610

Following gene: 239616608

Centisome position: 9.94

GC content: 47.66

Gene sequence:

>942_bases
ATGGAAAATATTGTGGTTACAGGCGGTGCAGGTTTTATAGGATCTCACGTTGTTAACGAGCTGATTGAGAAGAATATGGG
GCCCATCGTTATCGATAATCTTTCCTCAGGAAAAATCGAAAATCTCGACCCAAGAGCGTTGTTTTACCAGCAGGATATAA
CGGATCTCGAGATGATGGAACGCGTCTTCATGCTCCATAAGCCCCGTTATGTCTTTCACCTGGCGGCACAGATAAGCGTA
TCGCAATCCGTGAAAGATCCCATCGAAGATGCAAGGATCAACATATTAGGGACGTTGAACCTGCTCAACCTTTCGGTTAA
GTACGGCGTCGAGAAATTCATCTTTTCTTCTACCGGCGGCGCTATCTATGGAGATGATGTCTCGGAAATTCCCACATCGG
AGAAAGAATTCCCGAAGCCTTTTTCGCCGTACGGCATAGCCAAGTTTTCCGTTGAGAATTATCTGAGGTTTTACAGCGAG
CAGTACGGGCTCAAATACGTTGCGTTGAGATACGCCAACGTTTACGGCGAAAGGCAGGATCCTCACGGCGAGGCTGGCGT
GGTCGCGATCTTTTCAAAGAGGATGCTGGCTAACGACGAGGTCATTATCTTCGGTGACGGTGAAAATGTCCGGGATTATG
TTTATGCCGGCGATGTTGCACACGCGAACGTCCTCGCCATGGAGAAAGTGGAAAACGAAGTTATAAACATAGGCACGGGT
ATCGGAACATCTGTAAACGAACTCTTCGCTTTGTTGAAAGAGATTACAGGTTACCAGAAAGAACCGGTGTATGCTGACCC
GAGACCCGGCGATTTGAGAAAATCCATTTTGAAGTGGGACAAAGCGAAAGAGCTCATGGGCTGGGAACCCACTGTGAAAC
TCGAAGATGGGCTGGTGAAAACAGTTGAGTTCTTCAGAACAGAAATGGGTGCCTCTGAGTGA

Upstream 100 bases:

>100_bases
CCAAAAAGGCTTACCTGGGAGGATATACAGAAGGTGAGAAAAATGTACGATCAGGTGATAAACGGAAGAGAGATTTCTTT
GGAGGATTAGGAGGGTTAAA

Downstream 100 bases:

>100_bases
GCGCCTGAGGCCCCGCAACTTTGACGATCTGGTTGGCCAGGAACACCTTACCGGAAAAAACGGGATAATCCGCCGGGCAG
TAGAAAGCGGATATGTGTTC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MENIVVTGGAGFIGSHVVNELIEKNMGPIVIDNLSSGKIENLDPRALFYQQDITDLEMMERVFMLHKPRYVFHLAAQISV
SQSVKDPIEDARINILGTLNLLNLSVKYGVEKFIFSSTGGAIYGDDVSEIPTSEKEFPKPFSPYGIAKFSVENYLRFYSE
QYGLKYVALRYANVYGERQDPHGEAGVVAIFSKRMLANDEVIIFGDGENVRDYVYAGDVAHANVLAMEKVENEVINIGTG
IGTSVNELFALLKEITGYQKEPVYADPRPGDLRKSILKWDKAKELMGWEPTVKLEDGLVKTVEFFRTEMGASE

Sequences:

>Translated_313_residues
MENIVVTGGAGFIGSHVVNELIEKNMGPIVIDNLSSGKIENLDPRALFYQQDITDLEMMERVFMLHKPRYVFHLAAQISV
SQSVKDPIEDARINILGTLNLLNLSVKYGVEKFIFSSTGGAIYGDDVSEIPTSEKEFPKPFSPYGIAKFSVENYLRFYSE
QYGLKYVALRYANVYGERQDPHGEAGVVAIFSKRMLANDEVIIFGDGENVRDYVYAGDVAHANVLAMEKVENEVINIGTG
IGTSVNELFALLKEITGYQKEPVYADPRPGDLRKSILKWDKAKELMGWEPTVKLEDGLVKTVEFFRTEMGASE
>Mature_313_residues
MENIVVTGGAGFIGSHVVNELIEKNMGPIVIDNLSSGKIENLDPRALFYQQDITDLEMMERVFMLHKPRYVFHLAAQISV
SQSVKDPIEDARINILGTLNLLNLSVKYGVEKFIFSSTGGAIYGDDVSEIPTSEKEFPKPFSPYGIAKFSVENYLRFYSE
QYGLKYVALRYANVYGERQDPHGEAGVVAIFSKRMLANDEVIIFGDGENVRDYVYAGDVAHANVLAMEKVENEVINIGTG
IGTSVNELFALLKEITGYQKEPVYADPRPGDLRKSILKWDKAKELMGWEPTVKLEDGLVKTVEFFRTEMGASE

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=326, Percent_Identity=33.1288343558282, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI56237023, Length=327, Percent_Identity=29.3577981651376, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI56118217, Length=327, Percent_Identity=29.3577981651376, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI189083684, Length=327, Percent_Identity=29.3577981651376, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI42516563, Length=313, Percent_Identity=27.4760383386581, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1788353, Length=337, Percent_Identity=31.1572700296736, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786974, Length=321, Percent_Identity=32.0872274143302, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI48994969, Length=336, Percent_Identity=30.952380952381, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790049, Length=249, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788365, Length=327, Percent_Identity=22.6299694189602, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788366, Length=350, Percent_Identity=24, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71982035, Length=330, Percent_Identity=30.9090909090909, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71982038, Length=332, Percent_Identity=30.7228915662651, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17568069, Length=324, Percent_Identity=30.5555555555556, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI115532424, Length=322, Percent_Identity=28.8819875776398, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17539532, Length=318, Percent_Identity=25.4716981132075, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17539422, Length=332, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=332, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=332, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=332, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=332, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=329, Percent_Identity=32.2188449848024, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321437, Length=357, Percent_Identity=24.6498599439776, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356223, Length=321, Percent_Identity=27.1028037383178, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI19923002, Length=334, Percent_Identity=30.2395209580838, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24667531, Length=261, Percent_Identity=24.904214559387, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 34984; Mature: 34984

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIVVTGGAGFIGSHVVNELIEKNMGPIVIDNLSSGKIENLDPRALFYQQDITDLEMME
CCCEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHEEECCCCHHHHHHH
RVFMLHKPRYVFHLAAQISVSQSVKDPIEDARINILGTLNLLNLSVKYGVEKFIFSSTGG
HHHHHCCCCEEEEEEHHHHHHHHHHCCHHHCEEEEEEEEEEEEEHHHHCHHHHHEECCCC
AIYGDDVSEIPTSEKEFPKPFSPYGIAKFSVENYLRFYSEQYGLKYVALRYANVYGERQD
EEECCCHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCC
PHGEAGVVAIFSKRMLANDEVIIFGDGENVRDYVYAGDVAHANVLAMEKVENEVINIGTG
CCCCCCEEEEEHHHHHCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHEEECCC
IGTSVNELFALLKEITGYQKEPVYADPRPGDLRKSILKWDKAKELMGWEPTVKLEDGLVK
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCHHH
TVEFFRTEMGASE
HHHHHHHHCCCCC
>Mature Secondary Structure
MENIVVTGGAGFIGSHVVNELIEKNMGPIVIDNLSSGKIENLDPRALFYQQDITDLEMME
CCCEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHEEECCCCHHHHHHH
RVFMLHKPRYVFHLAAQISVSQSVKDPIEDARINILGTLNLLNLSVKYGVEKFIFSSTGG
HHHHHCCCCEEEEEEHHHHHHHHHHCCHHHCEEEEEEEEEEEEEHHHHCHHHHHEECCCC
AIYGDDVSEIPTSEKEFPKPFSPYGIAKFSVENYLRFYSEQYGLKYVALRYANVYGERQD
EEECCCHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCC
PHGEAGVVAIFSKRMLANDEVIIFGDGENVRDYVYAGDVAHANVLAMEKVENEVINIGTG
CCCCCCEEEEEHHHHHCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHEEECCC
IGTSVNELFALLKEITGYQKEPVYADPRPGDLRKSILKWDKAKELMGWEPTVKLEDGLVK
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCHHH
TVEFFRTEMGASE
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]