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The map label for this gene is ogt [H]

Identifier: 239616596

GI number: 239616596

Start: 210365

End: 210823

Strand: Reverse

Name: ogt [H]

Synonym: Kole_0188

Alternate gene names: 239616596

Gene position: 210823-210365 (Counterclockwise)

Preceding gene: 239616597

Following gene: 239616587

Centisome position: 9.16

GC content: 49.02

Gene sequence:

>459_bases
ATGGCCACGGAGCTTGGAGTTGTAACCACGGAGATAGGTGCTATTCTTTTGCATGCTGATTATGGAATGCTTTCGAGGAT
AGAGCTTGTGTTTGACAGCAAACTGGCTCCAAAGTCTCTGCCTCCTTTTACCACGCAAATCCAGGAATACCTCGAAGGGT
GCAGAAAAACCTTTGACTTTCCACTGTATTTGCCTTATGCCGGGTTTAAAAAGAAGGTTCTTTTGTACGTTAAGGAGATT
CCTTTTGGAAGGGTTGTCACCTACGGTGAGGTTGCAAAAGCGGCAGGAAGCCCGGGGGCTGCCAGGGCTGTTGGACAGAT
TATGAAGCGCAACCCGCTGCCCATTGTCATTCCGTGTCACAGGGTTGTTTCAGCGAGAGGTATCGGGGGGTTCAGTGCAG
GCCTTGAGTGGAAGCGTTTTCTTCTTGAACTTGAAGGTGTTTCCTTTGACGCCATATAG

Upstream 100 bases:

>100_bases
TATGTTCTTCATGGTATCACGATGACAGACGGAATTTCCAGAGTTATACCCGTTAAACTCGGGACCATCGAGTCCGTTAT
AGGTTGATTTTTTAGAGGTG

Downstream 100 bases:

>100_bases
CGATAGACATTCCCATTCCTACCAGTAATGCGCCAACGTAGAAGAAAAGCGAAGGGCGATGTTTCAAGAATATGATGTCG
CCTAAGATGGTGAAAACGGG

Product: methylated-DNA/protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MATELGVVTTEIGAILLHADYGMLSRIELVFDSKLAPKSLPPFTTQIQEYLEGCRKTFDFPLYLPYAGFKKKVLLYVKEI
PFGRVVTYGEVAKAAGSPGAARAVGQIMKRNPLPIVIPCHRVVSARGIGGFSAGLEWKRFLLELEGVSFDAI

Sequences:

>Translated_152_residues
MATELGVVTTEIGAILLHADYGMLSRIELVFDSKLAPKSLPPFTTQIQEYLEGCRKTFDFPLYLPYAGFKKKVLLYVKEI
PFGRVVTYGEVAKAAGSPGAARAVGQIMKRNPLPIVIPCHRVVSARGIGGFSAGLEWKRFLLELEGVSFDAI
>Mature_151_residues
ATELGVVTTEIGAILLHADYGMLSRIELVFDSKLAPKSLPPFTTQIQEYLEGCRKTFDFPLYLPYAGFKKKVLLYVKEIP
FGRVVTYGEVAKAAGSPGAARAVGQIMKRNPLPIVIPCHRVVSARGIGGFSAGLEWKRFLLELEGVSFDAI

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=118, Percent_Identity=40.6779661016949, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1787596, Length=106, Percent_Identity=37.7358490566038, Blast_Score=77, Evalue=5e-16,
Organism=Escherichia coli, GI1788542, Length=142, Percent_Identity=35.2112676056338, Blast_Score=70, Evalue=7e-14,
Organism=Escherichia coli, GI1786659, Length=80, Percent_Identity=46.25, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16647; Mature: 16516

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATELGVVTTEIGAILLHADYGMLSRIELVFDSKLAPKSLPPFTTQIQEYLEGCRKTFDF
CCCCCCHHHHHHCEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PLYLPYAGFKKKVLLYVKEIPFGRVVTYGEVAKAAGSPGAARAVGQIMKRNPLPIVIPCH
CEECCCCCCCHHEEEEEECCCCCCEEEHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECH
RVVSARGIGGFSAGLEWKRFLLELEGVSFDAI
HHHHHCCCCCCCCCHHHHHHHHHHCCCEECCC
>Mature Secondary Structure 
ATELGVVTTEIGAILLHADYGMLSRIELVFDSKLAPKSLPPFTTQIQEYLEGCRKTFDF
CCCCCHHHHHHCEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PLYLPYAGFKKKVLLYVKEIPFGRVVTYGEVAKAAGSPGAARAVGQIMKRNPLPIVIPCH
CEECCCCCCCHHEEEEEECCCCCCEEEHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECH
RVVSARGIGGFSAGLEWKRFLLELEGVSFDAI
HHHHHCCCCCCCCCHHHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]