Click here to switch to the map view.

The map label for this gene is nuoG [C]

Identifier: 239616580

GI number: 239616580

Start: 192345

End: 194099

Strand: Reverse

Name: nuoG [C]

Synonym: Kole_0172

Alternate gene names: 239616580

Gene position: 194099-192345 (Counterclockwise)

Preceding gene: 239616581

Following gene: 239616578

Centisome position: 8.43

GC content: 45.47

Gene sequence:

>1755_bases
GTGCCAGTTACCGTTACAATCAACGGAAAACAATATCAGGTTGAAGAAAATCGAACAGTCCTCGAAGTATGTCGTGAAAT
TGGTATCGAAATCCCAACGTTGTGTTATCATCCGGCGCTATCAATCGTGGGAGCATGCAGGGTTTGTATGGTTGAAATCG
AAGGAGCACGCAACCTTTCTCCAGCGTGTGCTACCTATGTTACTGATGGGATGGTCATAAGAACGAATACGGAGCGCGTG
AACCGCTCCGTGAGGTTCAATCTGGCTTTACTGCTTTCCAGCCATCCCAATGATTGTATGACATGTGATGCAAACGGGCG
CTGTGAATTCCAGGATCTCATTTACAGATACGATATCAAGGATATGTTCCCGAAGGACCTCAGAAATATACCCTACGACA
CGTCCAGCCCTTCTTTGTTCAGGGACATGAACAAGTGTATTCTTTGTGGAAGATGTGGCCGTGCCTGTAAAGAATTGCAA
GGACTTTCTATTCTTTCTGTGGCAGGAAGGGGATATCATTCTATACCAGTCCCAGCGCTTGGAGCTCCAATAGCTGCTAC
GGATTGTATCAACTGTGGCCAGTGCGCAACGGTGTGTCCGGTTGGAGCAATCACGGAGAGAATGGATTTCAGACGTGTAG
CCGAAGAACTGTATAAGCATGAAAAAGTTCTGGTGGTGCAGACAGCTCCGGCTGTTCGTGTAGCTCTCGGTGAAGAATTC
GGTGAAGAACCGGGAACGATCTCCACGGGTAAGATGGTTGCAGCCCTTAGAAAGTTAGGATTTGACTATGTTTTCGATAC
AGACTTTGCTGCTGACCTCACGATTATGGAAGAGGGATCCGAACTTCTTCAGAGGATAAAGGAAGGCGGTAAGTTCCCCA
TGTTCACTTCCTGTTGTCCCGGCTGGGTTAACCTGATGGAGAAACTCTATCCTGAATTCAGAGAAAATCTTTCCACAGCC
AAGTCTCCACATGAGATGCTCGGTGCTCTTATCAAAACCTATTTCGCCAAAAAGATAGGCAAAGATCCAAAAGATATATT
TGTCGTTTCCGTAATGCCATGTACAGCCAAGAAAGACGAAGCAGAGCGCGAAACATTGCTGGTTAGAGGGCATGTTGCTG
TGGATGCTGTTATAACCACTAGAGAATTAGGAAAATTGATAAAGATGAAAAAGATTCCTTATGAATCACTTCCTGAAGAA
GAATACGATACTCCTCTTGGCATATCTACCGGTGCCGCTGTAATATTTGGTGCTACCGGTGGTGTTATGGAAGCAGCTCT
CAGAACGGCTTATGAGCTTTACACCGGCAAAGAACTGCCGAAGCTCGATTTTGAATTTGTGCGTGGATTTGAGGGAGTCA
AAGAGGCTGAGGTTGACCTTGATGGAAAGATTCTGAAAGTTGCTGTTGCCCACGGTGGTGCAAATGTTGTCAAACTCATG
GATAAGATAAAATCTGGTGAGGTTTACTATGACTTTGTGGAGATTATGGCCTGTCCAGGTGGTTGCATTGGTGGTGGAGG
ACAGCCCAAATCCACCAGACCAGATTATCTTGAGAGAAGAATGCAGGCTATTTACGAAATTGATGAAAACATGCCTATCA
GAAAATCTCATGAAAACCCGGCGATCAAGAAAATCTACGAGGAATATCTTGAGCACCCGCTTAGCCACATATCCCATGAG
CTTCTCCACACGCACTATCTCGACAGGAAAAAGAGCGTTGTGGAACGAAAGAAAGAACTTGAAGAAGTAAATTGA

Upstream 100 bases:

>100_bases
CTTTATACATTGTAACTTCATAGAATGATATGGTAGAATAACCTTGATAGAAAATTCACAATAACTGTGAGTTTACGTTG
CCGAATGGAGGTGCTTCTGG

Downstream 100 bases:

>100_bases
GCTCAATGTGAGCGGGAGATAGATAATCGGGGACACTTTATCAAATGTCCCCGATTTTTTTATTAGCAGCCTAGAAATCT
TTAGACACCAGAGATTTTAT

Product: hydrogenase, Fe-only

Products: NA

Alternate protein names: CpI; Fe-only hydrogenase; [Fe] hydrogenase [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MPVTVTINGKQYQVEENRTVLEVCREIGIEIPTLCYHPALSIVGACRVCMVEIEGARNLSPACATYVTDGMVIRTNTERV
NRSVRFNLALLLSSHPNDCMTCDANGRCEFQDLIYRYDIKDMFPKDLRNIPYDTSSPSLFRDMNKCILCGRCGRACKELQ
GLSILSVAGRGYHSIPVPALGAPIAATDCINCGQCATVCPVGAITERMDFRRVAEELYKHEKVLVVQTAPAVRVALGEEF
GEEPGTISTGKMVAALRKLGFDYVFDTDFAADLTIMEEGSELLQRIKEGGKFPMFTSCCPGWVNLMEKLYPEFRENLSTA
KSPHEMLGALIKTYFAKKIGKDPKDIFVVSVMPCTAKKDEAERETLLVRGHVAVDAVITTRELGKLIKMKKIPYESLPEE
EYDTPLGISTGAAVIFGATGGVMEAALRTAYELYTGKELPKLDFEFVRGFEGVKEAEVDLDGKILKVAVAHGGANVVKLM
DKIKSGEVYYDFVEIMACPGGCIGGGGQPKSTRPDYLERRMQAIYEIDENMPIRKSHENPAIKKIYEEYLEHPLSHISHE
LLHTHYLDRKKSVVERKKELEEVN

Sequences:

>Translated_584_residues
MPVTVTINGKQYQVEENRTVLEVCREIGIEIPTLCYHPALSIVGACRVCMVEIEGARNLSPACATYVTDGMVIRTNTERV
NRSVRFNLALLLSSHPNDCMTCDANGRCEFQDLIYRYDIKDMFPKDLRNIPYDTSSPSLFRDMNKCILCGRCGRACKELQ
GLSILSVAGRGYHSIPVPALGAPIAATDCINCGQCATVCPVGAITERMDFRRVAEELYKHEKVLVVQTAPAVRVALGEEF
GEEPGTISTGKMVAALRKLGFDYVFDTDFAADLTIMEEGSELLQRIKEGGKFPMFTSCCPGWVNLMEKLYPEFRENLSTA
KSPHEMLGALIKTYFAKKIGKDPKDIFVVSVMPCTAKKDEAERETLLVRGHVAVDAVITTRELGKLIKMKKIPYESLPEE
EYDTPLGISTGAAVIFGATGGVMEAALRTAYELYTGKELPKLDFEFVRGFEGVKEAEVDLDGKILKVAVAHGGANVVKLM
DKIKSGEVYYDFVEIMACPGGCIGGGGQPKSTRPDYLERRMQAIYEIDENMPIRKSHENPAIKKIYEEYLEHPLSHISHE
LLHTHYLDRKKSVVERKKELEEVN
>Mature_583_residues
PVTVTINGKQYQVEENRTVLEVCREIGIEIPTLCYHPALSIVGACRVCMVEIEGARNLSPACATYVTDGMVIRTNTERVN
RSVRFNLALLLSSHPNDCMTCDANGRCEFQDLIYRYDIKDMFPKDLRNIPYDTSSPSLFRDMNKCILCGRCGRACKELQG
LSILSVAGRGYHSIPVPALGAPIAATDCINCGQCATVCPVGAITERMDFRRVAEELYKHEKVLVVQTAPAVRVALGEEFG
EEPGTISTGKMVAALRKLGFDYVFDTDFAADLTIMEEGSELLQRIKEGGKFPMFTSCCPGWVNLMEKLYPEFRENLSTAK
SPHEMLGALIKTYFAKKIGKDPKDIFVVSVMPCTAKKDEAERETLLVRGHVAVDAVITTRELGKLIKMKKIPYESLPEEE
YDTPLGISTGAAVIFGATGGVMEAALRTAYELYTGKELPKLDFEFVRGFEGVKEAEVDLDGKILKVAVAHGGANVVKLMD
KIKSGEVYYDFVEIMACPGGCIGGGGQPKSTRPDYLERRMQAIYEIDENMPIRKSHENPAIKKIYEEYLEHPLSHISHEL
LHTHYLDRKKSVVERKKELEEVN

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG4624

COG function: function code R; Iron only hydrogenase large subunit, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI11968051, Length=408, Percent_Identity=28.921568627451, Blast_Score=182, Evalue=9e-46,
Organism=Homo sapiens, GI6912524, Length=411, Percent_Identity=30.1703163017032, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI84452151, Length=384, Percent_Identity=30.7291666666667, Blast_Score=170, Evalue=4e-42,
Organism=Homo sapiens, GI134284357, Length=367, Percent_Identity=30.7901907356948, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI14165461, Length=457, Percent_Identity=29.7592997811816, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI33519475, Length=207, Percent_Identity=34.2995169082126, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI145693161, Length=263, Percent_Identity=28.5171102661597, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71995015, Length=396, Percent_Identity=32.3232323232323, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32566231, Length=252, Percent_Identity=30.952380952381, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17565758, Length=252, Percent_Identity=30.952380952381, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI193209088, Length=252, Percent_Identity=30.952380952381, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324089, Length=375, Percent_Identity=21.8666666666667, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI116007470, Length=407, Percent_Identity=30.4668304668305, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI116007466, Length=407, Percent_Identity=30.4668304668305, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI116007464, Length=407, Percent_Identity=30.4668304668305, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI116007468, Length=407, Percent_Identity=30.4668304668305, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI116007462, Length=285, Percent_Identity=32.6315789473684, Blast_Score=141, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24640559, Length=210, Percent_Identity=31.9047619047619, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24640557, Length=210, Percent_Identity=31.9047619047619, Blast_Score=112, Evalue=9e-25,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR009016
- InterPro:   IPR004108
- InterPro:   IPR003149
- InterPro:   IPR013352
- InterPro:   IPR001041 [H]

Pfam domain/function: PF02906 Fe_hyd_lg_C; PF02256 Fe_hyd_SSU [H]

EC number: =1.12.7.2 [H]

Molecular weight: Translated: 64972; Mature: 64841

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00641 COMPLEX1_75K_1 ; PS51085 2FE2S_FER_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTVTINGKQYQVEENRTVLEVCREIGIEIPTLCYHPALSIVGACRVCMVEIEGARNLS
CCEEEEECCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCC
PACATYVTDGMVIRTNTERVNRSVRFNLALLLSSHPNDCMTCDANGRCEFQDLIYRYDIK
HHHHHHHCCCEEEEECHHHHCCCCEEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHCHH
DMFPKDLRNIPYDTSSPSLFRDMNKCILCGRCGRACKELQGLSILSVAGRGYHSIPVPAL
HHCHHHHHCCCCCCCCCHHHHHHHHHEEHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
GAPIAATDCINCGQCATVCPVGAITERMDFRRVAEELYKHEKVLVVQTAPAVRVALGEEF
CCCCHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
GEEPGTISTGKMVAALRKLGFDYVFDTDFAADLTIMEEGSELLQRIKEGGKFPMFTSCCP
CCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCC
GWVNLMEKLYPEFRENLSTAKSPHEMLGALIKTYFAKKIGKDPKDIFVVSVMPCTAKKDE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
AERETLLVRGHVAVDAVITTRELGKLIKMKKIPYESLPEEEYDTPLGISTGAAVIFGATG
CCHHEEEEECCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCEEEEECCC
GVMEAALRTAYELYTGKELPKLDFEFVRGFEGVKEAEVDLDGKILKVAVAHGGANVVKLM
HHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHCCCCCCCCEEEEEEECCCHHHHHHH
DKIKSGEVYYDFVEIMACPGGCIGGGGQPKSTRPDYLERRMQAIYEIDENMPIRKSHENP
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCH
AIKKIYEEYLEHPLSHISHELLHTHYLDRKKSVVERKKELEEVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVTVTINGKQYQVEENRTVLEVCREIGIEIPTLCYHPALSIVGACRVCMVEIEGARNLS
CEEEEECCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCC
PACATYVTDGMVIRTNTERVNRSVRFNLALLLSSHPNDCMTCDANGRCEFQDLIYRYDIK
HHHHHHHCCCEEEEECHHHHCCCCEEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHCHH
DMFPKDLRNIPYDTSSPSLFRDMNKCILCGRCGRACKELQGLSILSVAGRGYHSIPVPAL
HHCHHHHHCCCCCCCCCHHHHHHHHHEEHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
GAPIAATDCINCGQCATVCPVGAITERMDFRRVAEELYKHEKVLVVQTAPAVRVALGEEF
CCCCHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
GEEPGTISTGKMVAALRKLGFDYVFDTDFAADLTIMEEGSELLQRIKEGGKFPMFTSCCP
CCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCC
GWVNLMEKLYPEFRENLSTAKSPHEMLGALIKTYFAKKIGKDPKDIFVVSVMPCTAKKDE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
AERETLLVRGHVAVDAVITTRELGKLIKMKKIPYESLPEEEYDTPLGISTGAAVIFGATG
CCHHEEEEECCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCEEEEECCC
GVMEAALRTAYELYTGKELPKLDFEFVRGFEGVKEAEVDLDGKILKVAVAHGGANVVKLM
HHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHCCCCCCCCEEEEEEECCCHHHHHHH
DKIKSGEVYYDFVEIMACPGGCIGGGGQPKSTRPDYLERRMQAIYEIDENMPIRKSHENP
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCH
AIKKIYEEYLEHPLSHISHELLHTHYLDRKKSVVERKKELEEVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1911757; 9836629; 10529166 [H]