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The map label for this gene is frvX [C]

Identifier: 239616450

GI number: 239616450

Start: 45631

End: 46665

Strand: Reverse

Name: frvX [C]

Synonym: Kole_0039

Alternate gene names: 239616450

Gene position: 46665-45631 (Counterclockwise)

Preceding gene: 239616451

Following gene: 239616449

Centisome position: 2.03

GC content: 47.25

Gene sequence:

>1035_bases
ATGAAGCTTGAGAGACTGAAAGAACTTTCCGAACTTCCCGGAATTTCAGGTTTTGAAGAGAAGGTCGCGGATTATATAGA
AGAAAAGATAAAGGATAAGGTCGATAAGTACTGGCGTGACAATGTGGGCAATCTTATTGCACTGAAGAAAGGAAAAGGTA
ACGGCAAGAAGCTCATGCTTCTGGCTCATATGGATGAGGTTGGGCTCATGGTCAAGAGGGTGAATGAAGACGGCACGCTG
GCGATAAGAGCTGTAGGTGGATTGGCTCCAAGGGTTCTGATCGGCAAGAAGGTGCTGGTGGGACCTGACCTTATCCCCGG
GGTAATTGGGTTCGATGCGATACATATTCAGGAACCCGCAACTGTCTTGAAGTCACCGAGTTACGATTCTTTATCTGTTT
ATCTTGGTGTTTCGAGTAAAGATGAAGTGAAGAACGTGAGAATTGGTGACCCTGTTTTTTTCAGCACGAAATATGAAGAG
GTCGGAAATTACGCGATAGGGAAGGCTTTCGATGACCGTAGCGGTTGTGAGGTTTTGCTTTCAGTTCTTGATGACATTCT
TGAAAATCCTGTTGACTATGATATCTACTTTGCCTGGGTTGTCCAGGAAGAGGTAGGGCTTAGAGGAAGCGGCGTTGCTG
CTGCCCAGATTAAACCCGATGCGGCTCTCGTTTTTGAGAATACCACCGCAGGAGACAACCCGGAAATGCCTGAGTCGAGG
TGGTCTACGCGCCTCGGTGAAGGGCCAGCGTTAACCTTTGCGCATTCAGGGCTGGTACTGGACAGGAAGATCTTCGAAAC
CATAGTCGATACGGCTAAAAGATATGACATTCCTTTCCAGTACAAAAGCAGAACAGTCGGCGGAACGGATGCCGCACGAC
TGGCGAGAACACTCAGAGGCATTCCTTCCGGGGTGATTTCAACCCCATCACGTTACATACATTCGCCTGTTTCAGTGATA
GATATCAATGATTTCTTGAAAGTTGTAAAACTTGCGAAAATCCTGGTTAGGGAAGGGAAGGTGTTAAGCGAATGA

Upstream 100 bases:

>100_bases
TTCATGCACACTCCTGTGGAAGTGGTGCAGGTATCTGATGTTGAAGAGTCTGCAAGATTACTCGTGAACTTCCTTTTTGA
ATTGGAAGAAGGTGAAGAGA

Downstream 100 bases:

>100_bases
AAGAATTGATAAAGAAAGTGACAGAGGTTTGTTCTCCAAGCGGCCGCGAGGATAAAATAAGGGAAATGATTATTGAGGAG
ATAAAGGATTTTGTTGATGG

Product: peptidase M42 family protein

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKLERLKELSELPGISGFEEKVADYIEEKIKDKVDKYWRDNVGNLIALKKGKGNGKKLMLLAHMDEVGLMVKRVNEDGTL
AIRAVGGLAPRVLIGKKVLVGPDLIPGVIGFDAIHIQEPATVLKSPSYDSLSVYLGVSSKDEVKNVRIGDPVFFSTKYEE
VGNYAIGKAFDDRSGCEVLLSVLDDILENPVDYDIYFAWVVQEEVGLRGSGVAAAQIKPDAALVFENTTAGDNPEMPESR
WSTRLGEGPALTFAHSGLVLDRKIFETIVDTAKRYDIPFQYKSRTVGGTDAARLARTLRGIPSGVISTPSRYIHSPVSVI
DINDFLKVVKLAKILVREGKVLSE

Sequences:

>Translated_344_residues
MKLERLKELSELPGISGFEEKVADYIEEKIKDKVDKYWRDNVGNLIALKKGKGNGKKLMLLAHMDEVGLMVKRVNEDGTL
AIRAVGGLAPRVLIGKKVLVGPDLIPGVIGFDAIHIQEPATVLKSPSYDSLSVYLGVSSKDEVKNVRIGDPVFFSTKYEE
VGNYAIGKAFDDRSGCEVLLSVLDDILENPVDYDIYFAWVVQEEVGLRGSGVAAAQIKPDAALVFENTTAGDNPEMPESR
WSTRLGEGPALTFAHSGLVLDRKIFETIVDTAKRYDIPFQYKSRTVGGTDAARLARTLRGIPSGVISTPSRYIHSPVSVI
DINDFLKVVKLAKILVREGKVLSE
>Mature_344_residues
MKLERLKELSELPGISGFEEKVADYIEEKIKDKVDKYWRDNVGNLIALKKGKGNGKKLMLLAHMDEVGLMVKRVNEDGTL
AIRAVGGLAPRVLIGKKVLVGPDLIPGVIGFDAIHIQEPATVLKSPSYDSLSVYLGVSSKDEVKNVRIGDPVFFSTKYEE
VGNYAIGKAFDDRSGCEVLLSVLDDILENPVDYDIYFAWVVQEEVGLRGSGVAAAQIKPDAALVFENTTAGDNPEMPESR
WSTRLGEGPALTFAHSGLVLDRKIFETIVDTAKRYDIPFQYKSRTVGGTDAARLARTLRGIPSGVISTPSRYIHSPVSVI
DINDFLKVVKLAKILVREGKVLSE

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=339, Percent_Identity=30.0884955752212, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI87082417, Length=345, Percent_Identity=30.7246376811594, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788727, Length=344, Percent_Identity=28.7790697674419, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 37719; Mature: 37719

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLERLKELSELPGISGFEEKVADYIEEKIKDKVDKYWRDNVGNLIALKKGKGNGKKLML
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEE
LAHMDEVGLMVKRVNEDGTLAIRAVGGLAPRVLIGKKVLVGPDLIPGVIGFDAIHIQEPA
EEECCHHHHHHEEECCCCCEEEEEECCCCCHHHHCCEEEECCCHHHHHHCCCEEEECCCH
TVLKSPSYDSLSVYLGVSSKDEVKNVRIGDPVFFSTKYEEVGNYAIGKAFDDRSGCEVLL
HHHCCCCCCCEEEEEECCCCHHHCEEECCCCEEEECCHHHHCCEECCCCCCCCHHHHHHH
SVLDDILENPVDYDIYFAWVVQEEVGLRGSGVAAAQIKPDAALVFENTTAGDNPEMPESR
HHHHHHHCCCCCCEEEEEEEEEHHHCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCHHH
WSTRLGEGPALTFAHSGLVLDRKIFETIVDTAKRYDIPFQYKSRTVGGTDAARLARTLRG
HHHCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHC
IPSGVISTPSRYIHSPVSVIDINDFLKVVKLAKILVREGKVLSE
CCCCHHCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLERLKELSELPGISGFEEKVADYIEEKIKDKVDKYWRDNVGNLIALKKGKGNGKKLML
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEE
LAHMDEVGLMVKRVNEDGTLAIRAVGGLAPRVLIGKKVLVGPDLIPGVIGFDAIHIQEPA
EEECCHHHHHHEEECCCCCEEEEEECCCCCHHHHCCEEEECCCHHHHHHCCCEEEECCCH
TVLKSPSYDSLSVYLGVSSKDEVKNVRIGDPVFFSTKYEEVGNYAIGKAFDDRSGCEVLL
HHHCCCCCCCEEEEEECCCCHHHCEEECCCCEEEECCHHHHCCEECCCCCCCCHHHHHHH
SVLDDILENPVDYDIYFAWVVQEEVGLRGSGVAAAQIKPDAALVFENTTAGDNPEMPESR
HHHHHHHCCCCCCEEEEEEEEEHHHCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCHHH
WSTRLGEGPALTFAHSGLVLDRKIFETIVDTAKRYDIPFQYKSRTVGGTDAARLARTLRG
HHHCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHC
IPSGVISTPSRYIHSPVSVIDINDFLKVVKLAKILVREGKVLSE
CCCCHHCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]