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The map label for this gene is frvX [H]

Identifier: 239616451

GI number: 239616451

Start: 46655

End: 47677

Strand: Reverse

Name: frvX [H]

Synonym: Kole_0040

Alternate gene names: 239616451

Gene position: 47677-46655 (Counterclockwise)

Preceding gene: 239616452

Following gene: 239616450

Centisome position: 2.07

GC content: 46.24

Gene sequence:

>1023_bases
TTGAAAACGCCCGAGCTATTGATCAAGCTATCAAATGCCTTTGGCCCTGTGGGTTATGAAGATGAGGTTAGAAAGATCAT
CCAGGAGGAGATCGAACCCTATGTGGATGAAGTAAAAGTCGACAACCTTGGCAACCTCATTGCCTTCAAACGGGGGACAG
GCAAGAAAAAAGTTGCATTTTTCGCTCACATCGATGAAGTCTCTCTTGTTGTTTCCGCCAAAGACGAAAGAGGTTTTTTA
AGGTTCGATACCATGGGCGGGATCGACCCAAAAGTCCTCATCGCTCAACGGGTAAAGGTTGTATCAAAGGACGGCAAAGT
GCGACGAGGAGTCATTGGAGTGCTTGCGCCACATCTCCAGAAAGCAGCAGATCGCAAAAACATACCGCCAATGGATGAAA
TGTTTATTGATGTTTCTATGAATCCGGATTATGAGAAAATCGAAATTGGTGATCTTGTTGTGATAGATACCGAAGCTTTT
GAAAGTAATGGAAAGGTTATTGGAAAAGCTCTTGATGATAGAGCCTGCGCTGTTATTTCTATACTCGTTGCGAAGCAGCT
TTCAAAGTTTTCCCGATTCCCGGATGTGTACTTCGTTTTTACGAGTCGCGAAGAGGTAGGCGGTATGGGAGCCAGAGTGG
CTGCTGAGGCGATACAACCTGATGTTGGGATAGCGATGGATGTAACCCACGCTCACAAAAGGACAGGAATAGAGCTCGGC
AAAGGACCGGCGATAACCGTAGGTGGTCCGAATATCCACAAAGGATACTTCAAAATGCTTGATGATGTGGCAAAACGCGA
AGGTGTCAAGGTTCAATACGAAATCGCAGCTGGGAGGACCGGAACAGATGCCGATAATGTCCAGATAGCCGGTACGGGAA
TCCCGGCGCTTCTGGTGTCGCTTCCAAATAAATTCATGCACACTCCTGTGGAAGTGGTGCAGGTATCTGATGTTGAAGAG
TCTGCAAGATTACTCGTGAACTTCCTTTTTGAATTGGAAGAAGGTGAAGAGAATGAAGCTTGA

Upstream 100 bases:

>100_bases
ATAATGTCTATGAAACCCCGGCTGTAGCCCACATCAGAACGGGAAGAATAGTTGTGGAAAAAATAGAGAGTGCAAAATTT
GAGGTAAAGGGGGGAAAGAT

Downstream 100 bases:

>100_bases
GAGACTGAAAGAACTTTCCGAACTTCCCGGAATTTCAGGTTTTGAAGAGAAGGTCGCGGATTATATAGAAGAAAAGATAA
AGGATAAGGTCGATAAGTAC

Product: peptidase M42 family protein

Products: D-glucose; GDP

Alternate protein names: TET; TET aminopeptidase; Leucyl aminopeptidase; PhTET2 [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKTPELLIKLSNAFGPVGYEDEVRKIIQEEIEPYVDEVKVDNLGNLIAFKRGTGKKKVAFFAHIDEVSLVVSAKDERGFL
RFDTMGGIDPKVLIAQRVKVVSKDGKVRRGVIGVLAPHLQKAADRKNIPPMDEMFIDVSMNPDYEKIEIGDLVVIDTEAF
ESNGKVIGKALDDRACAVISILVAKQLSKFSRFPDVYFVFTSREEVGGMGARVAAEAIQPDVGIAMDVTHAHKRTGIELG
KGPAITVGGPNIHKGYFKMLDDVAKREGVKVQYEIAAGRTGTDADNVQIAGTGIPALLVSLPNKFMHTPVEVVQVSDVEE
SARLLVNFLFELEEGEENEA

Sequences:

>Translated_340_residues
MKTPELLIKLSNAFGPVGYEDEVRKIIQEEIEPYVDEVKVDNLGNLIAFKRGTGKKKVAFFAHIDEVSLVVSAKDERGFL
RFDTMGGIDPKVLIAQRVKVVSKDGKVRRGVIGVLAPHLQKAADRKNIPPMDEMFIDVSMNPDYEKIEIGDLVVIDTEAF
ESNGKVIGKALDDRACAVISILVAKQLSKFSRFPDVYFVFTSREEVGGMGARVAAEAIQPDVGIAMDVTHAHKRTGIELG
KGPAITVGGPNIHKGYFKMLDDVAKREGVKVQYEIAAGRTGTDADNVQIAGTGIPALLVSLPNKFMHTPVEVVQVSDVEE
SARLLVNFLFELEEGEENEA
>Mature_340_residues
MKTPELLIKLSNAFGPVGYEDEVRKIIQEEIEPYVDEVKVDNLGNLIAFKRGTGKKKVAFFAHIDEVSLVVSAKDERGFL
RFDTMGGIDPKVLIAQRVKVVSKDGKVRRGVIGVLAPHLQKAADRKNIPPMDEMFIDVSMNPDYEKIEIGDLVVIDTEAF
ESNGKVIGKALDDRACAVISILVAKQLSKFSRFPDVYFVFTSREEVGGMGARVAAEAIQPDVGIAMDVTHAHKRTGIELG
KGPAITVGGPNIHKGYFKMLDDVAKREGVKVQYEIAAGRTGTDADNVQIAGTGIPALLVSLPNKFMHTPVEVVQVSDVEE
SARLLVNFLFELEEGEENEA

Specific function: Functions as an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. However, can also cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. Has neither carboxypeptidase nor endop

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=350, Percent_Identity=33.4285714285714, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI87082417, Length=367, Percent_Identity=27.2479564032698, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1788727, Length=344, Percent_Identity=26.453488372093, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 37177; Mature: 37177

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPELLIKLSNAFGPVGYEDEVRKIIQEEIEPYVDEVKVDNLGNLIAFKRGTGKKKVAF
CCCHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCEEEE
FAHIDEVSLVVSAKDERGFLRFDTMGGIDPKVLIAQRVKVVSKDGKVRRGVIGVLAPHLQ
EEEECEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
KAADRKNIPPMDEMFIDVSMNPDYEKIEIGDLVVIDTEAFESNGKVIGKALDDRACAVIS
HHHHCCCCCCHHHEEEEEECCCCCCEEEECCEEEEECHHHCCCCCEEEEHHCCHHHHHHH
ILVAKQLSKFSRFPDVYFVFTSREEVGGMGARVAAEAIQPDVGIAMDVTHAHKRTGIELG
HHHHHHHHHHHCCCCEEEEEECCHHHCCCCHHHHHHHHCCCCCEEEEEHHHHHHCCCEEC
KGPAITVGGPNIHKGYFKMLDDVAKREGVKVQYEIAAGRTGTDADNVQIAGTGIPALLVS
CCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEECCCHHHHHHH
LPNKFMHTPVEVVQVSDVEESARLLVNFLFELEEGEENEA
CCHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKTPELLIKLSNAFGPVGYEDEVRKIIQEEIEPYVDEVKVDNLGNLIAFKRGTGKKKVAF
CCCHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCEEEE
FAHIDEVSLVVSAKDERGFLRFDTMGGIDPKVLIAQRVKVVSKDGKVRRGVIGVLAPHLQ
EEEECEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
KAADRKNIPPMDEMFIDVSMNPDYEKIEIGDLVVIDTEAFESNGKVIGKALDDRACAVIS
HHHHCCCCCCHHHEEEEEECCCCCCEEEECCEEEEECHHHCCCCCEEEEHHCCHHHHHHH
ILVAKQLSKFSRFPDVYFVFTSREEVGGMGARVAAEAIQPDVGIAMDVTHAHKRTGIELG
HHHHHHHHHHHCCCCEEEEEECCHHHCCCCHHHHHHHHCCCCCEEEEEHHHHHHCCCEEC
KGPAITVGGPNIHKGYFKMLDDVAKREGVKVQYEIAAGRTGTDADNVQIAGTGIPALLVS
CCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEECCCHHHHHHH
LPNKFMHTPVEVVQVSDVEESARLLVNFLFELEEGEENEA
CCHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]