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The map label for this gene is pepT [H]

Identifier: 238925628

GI number: 238925628

Start: 3119112

End: 3120341

Strand: Direct

Name: pepT [H]

Synonym: EUBREC_3285

Alternate gene names: 238925628

Gene position: 3119112-3120341 (Clockwise)

Preceding gene: 238925626

Following gene: 238925629

Centisome position: 90.42

GC content: 49.27

Gene sequence:

>1230_bases
ATGAAAGTAGAAGAAAGATTATTAAAATATGTGGGCTATCACACCACATCTGATGAAGCAAGTGATAAAATACCAAGCAG
TGACAGGGAATTTGCACTCGCAAGAGAGCTTGAGTCAGAGCTTAAGGAGCTTGGCTTATCCAAGGTTGTGCTCACTGACC
ATTGTTACGTGTATGGTCTACTGCCTGCCACTGCCGGCATGGAACACAAAAAAGCGGTCGGATTTATTGCACATATGGAT
ACTGCTCCTGATTTTTCCGGGGAAAATGTCAAGCCACAGCTGCTGCGTCACTATGACGGAAGCGATGTGTTGCTTAGAGG
CAGTGGCGAATACCTGAGAGTAAGCGATTTTCCTGAGCTTAAAGCCCTCACCGGACGCACTCTCATTACCACAGATGGCA
CTACCCTGCTTGGTGCCGATGACAAGGCCGGTGTGGCTGAGATTATGACCGCCTTAGAGCAGATTATCGCAGAGGGCACT
CCTCACGGCGATATCTGGGTTGGATTTACCCCTGATGAAGAGGTCGGAAAGGGTGCGGATTTGTTTGATCTGGACTATTT
CGAGGCAGATTTTGCCTACACCGTGGACGGTGATTACGAGGGCGAGGTGGCCTATGAAAACTTTAATGCCGCAAGCGCTG
AATTTACCATAAAGGGCGTCAACGTGCATCCGGGCGAAGCAAAGGACATTATGGTCAATGCTGCTTTGGTTGGCTGCGAG
ATTGCCGCAGCGCTGCCTGCAAACGAGACTCCTGCCACAACTGAGGGCAGAGAGGGCTTTTACCATCTGTGTGATATGAG
CGGTGATGTAGCATCAGCCCATCTGTCATATATTGTGCGCGACCACGACAAGGCTCTTTTTGCTAAAAGACTTGATACTC
TCCGCTCTCTTGAGATAGAGATGAACAAAAAATATGGTGAGGGCACTGTTTCTTTGACTATCACCCACTCCTACGAGAAT
ATGCTTTCAATCATAATGGACAACATGTACATTATCGACCTGGCTAAGGCTGCTATAGAGGAAAACGGTCTAGAGCCGCT
ATCACGCCCGGTTCGCGGCGGTACAGACGGCGCACGTTTATCCTTCATGGGACTTCCTTGTCCAAACCTCGGTACCGGCG
GCTACGGCTTCCACGGCCCATTTGAGCACATATCAGTGGAGGGTATGGAGACTGCGGTTAATATAATCAAATCAATTGTG
AAGCATGTGCTGATGCTGGCTGACGCGTAA

Upstream 100 bases:

>100_bases
AGTTGATTTTACACCGTTTTAAGCTATATTATTTTCTATATACAGAGACTTTTTATTCCAGATTTCCAATCCAAAGTCTC
TCAGAAAGGATTTACTGATA

Downstream 100 bases:

>100_bases
TTTCGCTGCCGGTCGGTTACTGTTCACAACTAACAGTGTGACTAGCCACGGCCACGTTACTGTTCACAACTAACAGTATA
ATCAGCAACGCAGGTTAGTT

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKVEERLLKYVGYHTTSDEASDKIPSSDREFALARELESELKELGLSKVVLTDHCYVYGLLPATAGMEHKKAVGFIAHMD
TAPDFSGENVKPQLLRHYDGSDVLLRGSGEYLRVSDFPELKALTGRTLITTDGTTLLGADDKAGVAEIMTALEQIIAEGT
PHGDIWVGFTPDEEVGKGADLFDLDYFEADFAYTVDGDYEGEVAYENFNAASAEFTIKGVNVHPGEAKDIMVNAALVGCE
IAAALPANETPATTEGREGFYHLCDMSGDVASAHLSYIVRDHDKALFAKRLDTLRSLEIEMNKKYGEGTVSLTITHSYEN
MLSIIMDNMYIIDLAKAAIEENGLEPLSRPVRGGTDGARLSFMGLPCPNLGTGGYGFHGPFEHISVEGMETAVNIIKSIV
KHVLMLADA

Sequences:

>Translated_409_residues
MKVEERLLKYVGYHTTSDEASDKIPSSDREFALARELESELKELGLSKVVLTDHCYVYGLLPATAGMEHKKAVGFIAHMD
TAPDFSGENVKPQLLRHYDGSDVLLRGSGEYLRVSDFPELKALTGRTLITTDGTTLLGADDKAGVAEIMTALEQIIAEGT
PHGDIWVGFTPDEEVGKGADLFDLDYFEADFAYTVDGDYEGEVAYENFNAASAEFTIKGVNVHPGEAKDIMVNAALVGCE
IAAALPANETPATTEGREGFYHLCDMSGDVASAHLSYIVRDHDKALFAKRLDTLRSLEIEMNKKYGEGTVSLTITHSYEN
MLSIIMDNMYIIDLAKAAIEENGLEPLSRPVRGGTDGARLSFMGLPCPNLGTGGYGFHGPFEHISVEGMETAVNIIKSIV
KHVLMLADA
>Mature_409_residues
MKVEERLLKYVGYHTTSDEASDKIPSSDREFALARELESELKELGLSKVVLTDHCYVYGLLPATAGMEHKKAVGFIAHMD
TAPDFSGENVKPQLLRHYDGSDVLLRGSGEYLRVSDFPELKALTGRTLITTDGTTLLGADDKAGVAEIMTALEQIIAEGT
PHGDIWVGFTPDEEVGKGADLFDLDYFEADFAYTVDGDYEGEVAYENFNAASAEFTIKGVNVHPGEAKDIMVNAALVGCE
IAAALPANETPATTEGREGFYHLCDMSGDVASAHLSYIVRDHDKALFAKRLDTLRSLEIEMNKKYGEGTVSLTITHSYEN
MLSIIMDNMYIIDLAKAAIEENGLEPLSRPVRGGTDGARLSFMGLPCPNLGTGGYGFHGPFEHISVEGMETAVNIIKSIV
KHVLMLADA

Specific function: Cleaves the N-terminal amino acid of tripeptides [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=403, Percent_Identity=49.1315136476427, Blast_Score=377, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.11.4 [H]

Molecular weight: Translated: 44443; Mature: 44443

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVEERLLKYVGYHTTSDEASDKIPSSDREFALARELESELKELGLSKVVLTDHCYVYGL
CCHHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEE
LPATAGMEHKKAVGFIAHMDTAPDFSGENVKPQLLRHYDGSDVLLRGSGEYLRVSDFPEL
ECCCCCCCHHHHEEEEEECCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCEEEECCCCCH
KALTGRTLITTDGTTLLGADDKAGVAEIMTALEQIIAEGTPHGDIWVGFTPDEEVGKGAD
HHHCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCCC
LFDLDYFEADFAYTVDGDYEGEVAYENFNAASAEFTIKGVNVHPGEAKDIMVNAALVGCE
EEECCCCCCCEEEEECCCCCCCEEEECCCCCCCEEEEEECEECCCCCCEEEEEEEEEEEE
IAAALPANETPATTEGREGFYHLCDMSGDVASAHLSYIVRDHDKALFAKRLDTLRSLEIE
EEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
MNKKYGEGTVSLTITHSYENMLSIIMDNMYIIDLAKAAIEENGLEPLSRPVRGGTDGARL
ECCCCCCCEEEEEEEECHHHHHHHHHCCCCEEHHHHHHHHHCCCCHHHHCCCCCCCCCEE
SFMGLPCPNLGTGGYGFHGPFEHISVEGMETAVNIIKSIVKHVLMLADA
EEEECCCCCCCCCCCCCCCCHHHEEECHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVEERLLKYVGYHTTSDEASDKIPSSDREFALARELESELKELGLSKVVLTDHCYVYGL
CCHHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEE
LPATAGMEHKKAVGFIAHMDTAPDFSGENVKPQLLRHYDGSDVLLRGSGEYLRVSDFPEL
ECCCCCCCHHHHEEEEEECCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCEEEECCCCCH
KALTGRTLITTDGTTLLGADDKAGVAEIMTALEQIIAEGTPHGDIWVGFTPDEEVGKGAD
HHHCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCCC
LFDLDYFEADFAYTVDGDYEGEVAYENFNAASAEFTIKGVNVHPGEAKDIMVNAALVGCE
EEECCCCCCCEEEEECCCCCCCEEEECCCCCCCEEEEEECEECCCCCCEEEEEEEEEEEE
IAAALPANETPATTEGREGFYHLCDMSGDVASAHLSYIVRDHDKALFAKRLDTLRSLEIE
EEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
MNKKYGEGTVSLTITHSYENMLSIIMDNMYIIDLAKAAIEENGLEPLSRPVRGGTDGARL
ECCCCCCCEEEEEEEECHHHHHHHHHCCCCEEHHHHHHHHHCCCCHHHHCCCCCCCCCEE
SFMGLPCPNLGTGGYGFHGPFEHISVEGMETAVNIIKSIVKHVLMLADA
EEEECCCCCCCCCCCCCCCCHHHEEECHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]