Click here to switch to the map view.

The map label for this gene is trmB [H]

Identifier: 238925626

GI number: 238925626

Start: 3117341

End: 3117982

Strand: Direct

Name: trmB [H]

Synonym: EUBREC_3282

Alternate gene names: 238925626

Gene position: 3117341-3117982 (Clockwise)

Preceding gene: 238925587

Following gene: 238925628

Centisome position: 90.37

GC content: 45.79

Gene sequence:

>642_bases
ATGAGACTTAGAAATATCCCTGGCGCCGGTGAAGTCATCGACAACAGCCCTTATTGCATAAAGGAGCCTGTTGAATTAAA
AGGAAAATGGCATGATTTTTTAGGAAACAATAATCCGATTCATATAGAGGTTGGTATGGGAAAGGGCAGATTTCTGATGG
ATCTGGCACGGCTTCATCCCGAGATTAACTATATAGGTATCGAACGATATACCAGTGTGCTTCTTCGTGCTGTACAAAAA
ATGCAGGAGGACGAGCTGCCAAATGTGCACTTTTTGTGCATTGATGCCGCAACTCTTCCGGATATTTTTGACCGTAATGA
GGTTGACCGCATTTACCTGAACTTTTCCGATCCATGGCCGAAGGACAGGCACGCAAGAAGACGCCTCACCTCAAGCGAAT
TTCTTGCCAGATATGATTTGTTTCTCGCACCTGACGGACGCATAGAGTTCAAGACTGACAACCAGGACCTCTTCACCTTC
TCTCTGGAAGAGATTGAAAGCTCTGATAAATGGCACCTCGACGCCAGTACACGCGACCTGCATCATGATGCTGCAATGAA
TGAGGGCAACATCATGACCGAGTACGAGGAGAAATTCTCTGCTGTCGGAAACCCTATCTGCAAGCTGATTGCTTCAAGAT
AA

Upstream 100 bases:

>100_bases
TCAAGGGCATGTGCATCGTATTTTTCCATTTTATTTTGACACCCACACAATTGTAGGGTATTATCTATATGAAATTTTAC
GTTTTTATAAGGAGATTTAC

Downstream 100 bases:

>100_bases
TGCAACATATCTGTCGTTAATCGCATGATTTATATAGTCAAACATGACCCAGCTTTTATGACATTCTTCAGATTTTTCTG
AGGAACTGTCATATTCGTCT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MRLRNIPGAGEVIDNSPYCIKEPVELKGKWHDFLGNNNPIHIEVGMGKGRFLMDLARLHPEINYIGIERYTSVLLRAVQK
MQEDELPNVHFLCIDAATLPDIFDRNEVDRIYLNFSDPWPKDRHARRRLTSSEFLARYDLFLAPDGRIEFKTDNQDLFTF
SLEEIESSDKWHLDASTRDLHHDAAMNEGNIMTEYEEKFSAVGNPICKLIASR

Sequences:

>Translated_213_residues
MRLRNIPGAGEVIDNSPYCIKEPVELKGKWHDFLGNNNPIHIEVGMGKGRFLMDLARLHPEINYIGIERYTSVLLRAVQK
MQEDELPNVHFLCIDAATLPDIFDRNEVDRIYLNFSDPWPKDRHARRRLTSSEFLARYDLFLAPDGRIEFKTDNQDLFTF
SLEEIESSDKWHLDASTRDLHHDAAMNEGNIMTEYEEKFSAVGNPICKLIASR
>Mature_213_residues
MRLRNIPGAGEVIDNSPYCIKEPVELKGKWHDFLGNNNPIHIEVGMGKGRFLMDLARLHPEINYIGIERYTSVLLRAVQK
MQEDELPNVHFLCIDAATLPDIFDRNEVDRIYLNFSDPWPKDRHARRRLTSSEFLARYDLFLAPDGRIEFKTDNQDLFTF
SLEEIESSDKWHLDASTRDLHHDAAMNEGNIMTEYEEKFSAVGNPICKLIASR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=182, Percent_Identity=25.8241758241758, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 24678; Mature: 24678

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRNIPGAGEVIDNSPYCIKEPVELKGKWHDFLGNNNPIHIEVGMGKGRFLMDLARLHP
CCCCCCCCCCCEECCCCCEECCCHHHCCCCHHHCCCCCCEEEEEECCCCHHHHHHHHHCC
EINYIGIERYTSVLLRAVQKMQEDELPNVHFLCIDAATLPDIFDRNEVDRIYLNFSDPWP
CCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCCCCEEEEEECCCCCC
KDRHARRRLTSSEFLARYDLFLAPDGRIEFKTDNQDLFTFSLEEIESSDKWHLDASTRDL
CCHHHHHHCCHHHHHHHEEEEECCCCEEEEEECCCEEEEEEHHHHCCCCCEEECCCCHHH
HHDAAMNEGNIMTEYEEKFSAVGNPICKLIASR
HHHHCCCCCCEEEHHHHHHHHHCHHHHHHHCCC
>Mature Secondary Structure
MRLRNIPGAGEVIDNSPYCIKEPVELKGKWHDFLGNNNPIHIEVGMGKGRFLMDLARLHP
CCCCCCCCCCCEECCCCCEECCCHHHCCCCHHHCCCCCCEEEEEECCCCHHHHHHHHHCC
EINYIGIERYTSVLLRAVQKMQEDELPNVHFLCIDAATLPDIFDRNEVDRIYLNFSDPWP
CCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCCCCEEEEEECCCCCC
KDRHARRRLTSSEFLARYDLFLAPDGRIEFKTDNQDLFTFSLEEIESSDKWHLDASTRDL
CCHHHHHHCCHHHHHHHEEEEECCCCEEEEEECCCEEEEEEHHHHCCCCCEEECCCCHHH
HHDAAMNEGNIMTEYEEKFSAVGNPICKLIASR
HHHHCCCCCCEEEHHHHHHHHHCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA