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The map label for this gene is cysW [H]

Identifier: 238924823

GI number: 238924823

Start: 2342505

End: 2343395

Strand: Reverse

Name: cysW [H]

Synonym: EUBREC_2474

Alternate gene names: 238924823

Gene position: 2343395-2342505 (Counterclockwise)

Preceding gene: 238924824

Following gene: 238924822

Centisome position: 67.93

GC content: 43.88

Gene sequence:

>891_bases
ATGCGATGCACACAAACAGGAAAGGAGCACAATGCGATGACCGAACAGGTAAAGCTTGAAAGAAGAAAAAAGATCACAAA
GATAATACTCGTCACTGTCACGGTGCTCTTCATATTTGTCATGCTTGTAGTTCCGCTTTTTTCTGTGATGGCAAGTGCTT
TAAAGGAGGGGCTTGGATTCTATCTGAAAGCTATCTCCACAAGCTACGTGCTGTCAGCACTCAAGGTGACTTTGATAGCA
ACAGTTTTTGCGGTAGTTATCAACACCTTCTTTGGAATATGGACAGCGTGGCTGTTGACCAGGTTTTCGTTTAAAGGAAA
GCAGGTGCTTGCAACACTTATTGATATACCGTTTTCAATTTCTCCGGTCATCGTAGGACTTGCGTATCTTATGACCTTCG
GACGGCTTGGATGGTTTTATCCGGTTATCAGATGGTTTAATGAGATATTCGGCACGAATGTGAGAATCACCTTTGCAATA
CCGGGAGTTGTGCTTGCTACGATATTTGTCACGTTTCCGTATGTGTCAAGGGAAATTATACCGGTGCTCAATGCAGAGGG
AAAGGATGAGGAAGAAGCCGCAGCGCTGATGGGAGCATCCGGCTTTACGATATTTCGGAAAATAACACTTCCGCAGATGA
AGTGGAGCCTGATTTATGGAATCATACTTTGTACGGCGAGAGCCCTCGGCGAGTTCGGCGCGGTAAATGCGCTCTCGAAG
ACAAGAGGCGAGACATTTACACTGCCGCTTGAGATTGATGCCTTGTATATGTCGGGCACAGACAGCTCGATAACATCGGC
ATTTGCTGTATCGTCGATACTGTTAATTATTGCATTAATCATTCTTTTTGCAAGAAATATAGTTGAGCATAAATTTAAGA
AATTACACTGA

Upstream 100 bases:

>100_bases
ATGCTTGTCATATCATTTTTACTGCTGTTATTTGTAAACATAGTGCAGGTTAAACAGTCACAGCGCACTAATTTGTTGTA
GAAGGCTGCATATAAATCAA

Downstream 100 bases:

>100_bases
GATTTATGGGAAATATGATTTACACTAATGAAATTTGTTACTGCTTACACACCGGTGTGGCAGACAGCTATAGAGTGGCT
GTACTAAGAAAGCAGGAAGG

Product: ABC sulfate/thiosulfate transporter, inner membrane subunit, CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRCTQTGKEHNAMTEQVKLERRKKITKIILVTVTVLFIFVMLVVPLFSVMASALKEGLGFYLKAISTSYVLSALKVTLIA
TVFAVVINTFFGIWTAWLLTRFSFKGKQVLATLIDIPFSISPVIVGLAYLMTFGRLGWFYPVIRWFNEIFGTNVRITFAI
PGVVLATIFVTFPYVSREIIPVLNAEGKDEEEAAALMGASGFTIFRKITLPQMKWSLIYGIILCTARALGEFGAVNALSK
TRGETFTLPLEIDALYMSGTDSSITSAFAVSSILLIIALIILFARNIVEHKFKKLH

Sequences:

>Translated_296_residues
MRCTQTGKEHNAMTEQVKLERRKKITKIILVTVTVLFIFVMLVVPLFSVMASALKEGLGFYLKAISTSYVLSALKVTLIA
TVFAVVINTFFGIWTAWLLTRFSFKGKQVLATLIDIPFSISPVIVGLAYLMTFGRLGWFYPVIRWFNEIFGTNVRITFAI
PGVVLATIFVTFPYVSREIIPVLNAEGKDEEEAAALMGASGFTIFRKITLPQMKWSLIYGIILCTARALGEFGAVNALSK
TRGETFTLPLEIDALYMSGTDSSITSAFAVSSILLIIALIILFARNIVEHKFKKLH
>Mature_296_residues
MRCTQTGKEHNAMTEQVKLERRKKITKIILVTVTVLFIFVMLVVPLFSVMASALKEGLGFYLKAISTSYVLSALKVTLIA
TVFAVVINTFFGIWTAWLLTRFSFKGKQVLATLIDIPFSISPVIVGLAYLMTFGRLGWFYPVIRWFNEIFGTNVRITFAI
PGVVLATIFVTFPYVSREIIPVLNAEGKDEEEAAALMGASGFTIFRKITLPQMKWSLIYGIILCTARALGEFGAVNALSK
TRGETFTLPLEIDALYMSGTDSSITSAFAVSSILLIIALIILFARNIVEHKFKKLH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=287, Percent_Identity=40.0696864111498, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788764, Length=175, Percent_Identity=36, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786980, Length=215, Percent_Identity=35.3488372093023, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32915; Mature: 32915

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCTQTGKEHNAMTEQVKLERRKKITKIILVTVTVLFIFVMLVVPLFSVMASALKEGLGF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLKAISTSYVLSALKVTLIATVFAVVINTFFGIWTAWLLTRFSFKGKQVLATLIDIPFSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCH
SPVIVGLAYLMTFGRLGWFYPVIRWFNEIFGTNVRITFAIPGVVLATIFVTFPYVSREII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
PVLNAEGKDEEEAAALMGASGFTIFRKITLPQMKWSLIYGIILCTARALGEFGAVNALSK
HHHCCCCCCHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRGETFTLPLEIDALYMSGTDSSITSAFAVSSILLIIALIILFARNIVEHKFKKLH
CCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRCTQTGKEHNAMTEQVKLERRKKITKIILVTVTVLFIFVMLVVPLFSVMASALKEGLGF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLKAISTSYVLSALKVTLIATVFAVVINTFFGIWTAWLLTRFSFKGKQVLATLIDIPFSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCH
SPVIVGLAYLMTFGRLGWFYPVIRWFNEIFGTNVRITFAIPGVVLATIFVTFPYVSREII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
PVLNAEGKDEEEAAALMGASGFTIFRKITLPQMKWSLIYGIILCTARALGEFGAVNALSK
HHHCCCCCCHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRGETFTLPLEIDALYMSGTDSSITSAFAVSSILLIIALIILFARNIVEHKFKKLH
CCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]