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The map label for this gene is miaB [H]

Identifier: 238924014

GI number: 238924014

Start: 1538482

End: 1539921

Strand: Direct

Name: miaB [H]

Synonym: EUBREC_1652

Alternate gene names: 238924014

Gene position: 1538482-1539921 (Clockwise)

Preceding gene: 238924013

Following gene: 238924015

Centisome position: 44.6

GC content: 43.33

Gene sequence:

>1440_bases
ATGAGCTACGATATAGATATAACAAAGACACCACCGGCACATGAGCCTGAGAGGCAGTATTACTACATGGCAAAGGCAAA
GGATTTTGTTGAAAAGAAATCTAAAGAAATCGGCCGTCCTATGACTTATTTTGTAAAGACATTCGGGTGTCAGATGAATG
CCCGCGACTCAGAAAAGCTTGTCGGAATACTCGAGCAGATAGGCTACGTGGAAGGAACTGATGAGCACTCTGATTTCATT
GTATACAACACCTGTACCGTACGTGAGAATGCCAATAATAAGGTGTATGGCAGGCTTGGATATCTGCAGAACTACAAGAA
AAAGAATCCTCTGATGAAGATTGCTCTCTGCGGATGCATGATGCAGGAGCCGGAGGTTGTTGAAAATATCAAAAAGCACT
ATAAGTTTGTAGATATCGTATTTGGAACCCACAATATATTCAAGTTTGCAGAGATTTTGTGCAACAATATCGAGTCTGGC
TCACAGGTGATTGACATATGGAAGGATACCAATCAGATTGTCGAAGATCTGCCGGTGAAGAGAAAGTTCTCTTTCAAATC
AGGTGTCAATATCATGTTTGGCTGCAACAATTTCTGCAGCTACTGTATCGTGCCATACGTAAGAGGTCGTGAGAGAAGCA
GGGAGCCTAAGGATATCATAAGAGAGATAGAAAAGCTTGTTGCAGACGGAGTGTGCGAGATAATGCTTCTCGGACAGAAT
GTCAACTCATACGGTAAAACTCTTGACAATCCTATAACCTTTGCAGAGCTTCTTCGCGAGGTCAATAAGATTGAGGGACT
TAAGAGGATTCGTTTTATGACATCTCATCCAAAGGATTTATCCGATGACCTGATTATGGCGATAAAGGAGTGCGACAAGG
TGTGCAAGCATATGCATCTGCCGCTTCAGTCAGGTAGCTCAAGGGTGCTTAAGGAGATGAACAGGCACTATGACAAGGAA
AAATATCTCGACGAGGTAAAGAGACTTCGTGAGCAGATTCCTGATATTGCAATCACAACTGATATCATTGTGGGCTTCCC
GGGAGAGACTGAGGAGGATTTCCTTGAGACAATGGATGTTGTTAAGCAGGTCAGATATGACAGTGCTTTTACATTTATTT
ACTCAAAGAGAACAGGCACACGCGCAGCTACCATGGAGAATCAGGTGCCGGATGATGTGGTCAAGGACAGGTTTGACAGG
CTCTTAAAGGAGGTTCAGACTATCTCATCAGAAAAGGCAAAATGCTATGAGGGCAAAGTGGTGCCGGTACTTGCAGAGGA
GATGGATGACCAGAAGGATGGCTATGTGACCGGACGTATGGACAATAATTCAATCGTACATTTTCCGGGTACCGAGGATA
TGATTGGAAATATCTACAATGTGAAGCTTGATGAGTGCAGAGGCTTTTACTATATGGGAGAGATAAAAGAGGATGCGTAA

Upstream 100 bases:

>100_bases
CACTGACTAGCTACAAATATATTATTTACGGCAGTGGTCAGATCAGGGAATAGTCAGGTAAAGTATCATAGAATTAATTT
GAGAGGAAATATAATATAAC

Downstream 100 bases:

>100_bases
GCGTTTTGGCAGAAATGATATAATCCTGATATCGGTTATTGTAATTATCTCTGTCGCTCTGCTTCTTATATGGAAGCTTG
TATATTTAAAGGGACAGGAT

Product: tRNA-i(6)A37 thiotransferase enzyme MiaB

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSYDIDITKTPPAHEPERQYYYMAKAKDFVEKKSKEIGRPMTYFVKTFGCQMNARDSEKLVGILEQIGYVEGTDEHSDFI
VYNTCTVRENANNKVYGRLGYLQNYKKKNPLMKIALCGCMMQEPEVVENIKKHYKFVDIVFGTHNIFKFAEILCNNIESG
SQVIDIWKDTNQIVEDLPVKRKFSFKSGVNIMFGCNNFCSYCIVPYVRGRERSREPKDIIREIEKLVADGVCEIMLLGQN
VNSYGKTLDNPITFAELLREVNKIEGLKRIRFMTSHPKDLSDDLIMAIKECDKVCKHMHLPLQSGSSRVLKEMNRHYDKE
KYLDEVKRLREQIPDIAITTDIIVGFPGETEEDFLETMDVVKQVRYDSAFTFIYSKRTGTRAATMENQVPDDVVKDRFDR
LLKEVQTISSEKAKCYEGKVVPVLAEEMDDQKDGYVTGRMDNNSIVHFPGTEDMIGNIYNVKLDECRGFYYMGEIKEDA

Sequences:

>Translated_479_residues
MSYDIDITKTPPAHEPERQYYYMAKAKDFVEKKSKEIGRPMTYFVKTFGCQMNARDSEKLVGILEQIGYVEGTDEHSDFI
VYNTCTVRENANNKVYGRLGYLQNYKKKNPLMKIALCGCMMQEPEVVENIKKHYKFVDIVFGTHNIFKFAEILCNNIESG
SQVIDIWKDTNQIVEDLPVKRKFSFKSGVNIMFGCNNFCSYCIVPYVRGRERSREPKDIIREIEKLVADGVCEIMLLGQN
VNSYGKTLDNPITFAELLREVNKIEGLKRIRFMTSHPKDLSDDLIMAIKECDKVCKHMHLPLQSGSSRVLKEMNRHYDKE
KYLDEVKRLREQIPDIAITTDIIVGFPGETEEDFLETMDVVKQVRYDSAFTFIYSKRTGTRAATMENQVPDDVVKDRFDR
LLKEVQTISSEKAKCYEGKVVPVLAEEMDDQKDGYVTGRMDNNSIVHFPGTEDMIGNIYNVKLDECRGFYYMGEIKEDA
>Mature_478_residues
SYDIDITKTPPAHEPERQYYYMAKAKDFVEKKSKEIGRPMTYFVKTFGCQMNARDSEKLVGILEQIGYVEGTDEHSDFIV
YNTCTVRENANNKVYGRLGYLQNYKKKNPLMKIALCGCMMQEPEVVENIKKHYKFVDIVFGTHNIFKFAEILCNNIESGS
QVIDIWKDTNQIVEDLPVKRKFSFKSGVNIMFGCNNFCSYCIVPYVRGRERSREPKDIIREIEKLVADGVCEIMLLGQNV
NSYGKTLDNPITFAELLREVNKIEGLKRIRFMTSHPKDLSDDLIMAIKECDKVCKHMHLPLQSGSSRVLKEMNRHYDKEK
YLDEVKRLREQIPDIAITTDIIVGFPGETEEDFLETMDVVKQVRYDSAFTFIYSKRTGTRAATMENQVPDDVVKDRFDRL
LKEVQTISSEKAKCYEGKVVPVLAEEMDDQKDGYVTGRMDNNSIVHFPGTEDMIGNIYNVKLDECRGFYYMGEIKEDA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=447, Percent_Identity=36.0178970917226, Blast_Score=282, Evalue=6e-76,
Organism=Homo sapiens, GI28872784, Length=447, Percent_Identity=36.0178970917226, Blast_Score=281, Evalue=8e-76,
Organism=Homo sapiens, GI93277076, Length=444, Percent_Identity=28.6036036036036, Blast_Score=159, Evalue=8e-39,
Organism=Escherichia coli, GI1786882, Length=423, Percent_Identity=39.0070921985816, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1787057, Length=409, Percent_Identity=28.361858190709, Blast_Score=165, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=439, Percent_Identity=32.1184510250569, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI71996771, Length=251, Percent_Identity=30.2788844621514, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21356207, Length=445, Percent_Identity=34.6067415730337, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI19922432, Length=447, Percent_Identity=29.3064876957494, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 55378; Mature: 55247

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYDIDITKTPPAHEPERQYYYMAKAKDFVEKKSKEIGRPMTYFVKTFGCQMNARDSEKL
CCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHH
VGILEQIGYVEGTDEHSDFIVYNTCTVRENANNKVYGRLGYLQNYKKKNPLMKIALCGCM
HHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCEEEEHHHHHHHHHCCCHHHHHHHHHH
MQEPEVVENIKKHYKFVDIVFGTHNIFKFAEILCNNIESGSQVIDIWKDTNQIVEDLPVK
CCCCHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCCCC
RKFSFKSGVNIMFGCNNFCSYCIVPYVRGRERSREPKDIIREIEKLVADGVCEIMLLGQN
CEECCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHEEEEECCC
VNSYGKTLDNPITFAELLREVNKIEGLKRIRFMTSHPKDLSDDLIMAIKECDKVCKHMHL
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
PLQSGSSRVLKEMNRHYDKEKYLDEVKRLREQIPDIAITTDIIVGFPGETEEDFLETMDV
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHH
VKQVRYDSAFTFIYSKRTGTRAATMENQVPDDVVKDRFDRLLKEVQTISSEKAKCYEGKV
HHHHHHCCEEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCE
VPVLAEEMDDQKDGYVTGRMDNNSIVHFPGTEDMIGNIYNVKLDECRGFYYMGEIKEDA
EEHHHHHCCCCCCCEEEEEECCCCEEECCCCHHHHCCEEEEEHHHHCCEEEECCCCCCC
>Mature Secondary Structure 
SYDIDITKTPPAHEPERQYYYMAKAKDFVEKKSKEIGRPMTYFVKTFGCQMNARDSEKL
CCCCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHH
VGILEQIGYVEGTDEHSDFIVYNTCTVRENANNKVYGRLGYLQNYKKKNPLMKIALCGCM
HHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCEEEEHHHHHHHHHCCCHHHHHHHHHH
MQEPEVVENIKKHYKFVDIVFGTHNIFKFAEILCNNIESGSQVIDIWKDTNQIVEDLPVK
CCCCHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCCCC
RKFSFKSGVNIMFGCNNFCSYCIVPYVRGRERSREPKDIIREIEKLVADGVCEIMLLGQN
CEECCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHEEEEECCC
VNSYGKTLDNPITFAELLREVNKIEGLKRIRFMTSHPKDLSDDLIMAIKECDKVCKHMHL
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
PLQSGSSRVLKEMNRHYDKEKYLDEVKRLREQIPDIAITTDIIVGFPGETEEDFLETMDV
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHH
VKQVRYDSAFTFIYSKRTGTRAATMENQVPDDVVKDRFDRLLKEVQTISSEKAKCYEGKV
HHHHHHCCEEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCE
VPVLAEEMDDQKDGYVTGRMDNNSIVHFPGTEDMIGNIYNVKLDECRGFYYMGEIKEDA
EEHHHHHCCCCCCCEEEEEECCCCEEECCCCHHHHCCEEEEEHHHHCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA