Click here to switch to the map view.

The map label for this gene is dacB [H]

Identifier: 238923987

GI number: 238923987

Start: 1515010

End: 1516164

Strand: Direct

Name: dacB [H]

Synonym: EUBREC_1625

Alternate gene names: 238923987

Gene position: 1515010-1516164 (Clockwise)

Preceding gene: 238923986

Following gene: 238923988

Centisome position: 43.92

GC content: 39.83

Gene sequence:

>1155_bases
ATGCAAATAATCATATATATTACCGATGGAGGTAATTATATGGTTATTTTTTTGAAAAAATATAAGTCGAAAATAGCTTT
AATGGTTTTCATTTGTGTGTTGGTTTGTACTGTCAGCTGTTTGCTTGATTTTAAGACGCTGGAAAAGCAAAAAATGCCCG
GTTTGGAAAAGAAAAGTGTGTTTATGGCAGCTGAAAATACTGTTGCTAAGAATACTGACAAAGCCGTAAATGAACCCAGG
CTTCATGCTGTTTCAGCGGCATTATATGATGCGGATGACGAGACGTTTATATATGGTAGAAATATGAGAGACAGCATGGC
AAATGCCAGCACCACGAAGCTTCTTACGTGCATTGTGGCACTTGAAAAATCTGATATAAATGATACTGTTACGATTTCAC
AAAATGCTGCATCACAGCCTGCGGTCAAGCTTGGACTTGTGGCCGGGAATAGCTATAATATGAAGGATTTACTCTATGGC
ATGATGCTTGAGTCGTTCAATGACTGTGCCTATGCGATTGCGGAGCATGTGGGCGGCTCGACGGAAGGCTTTGCAAAGCT
TATGAATGAAAAAGCAAATGAGATAGGGTGCCTTGGCACGTATTTTATTACACCAAACGGGCTGGATGCTGAGAATGATA
TAAGCTTTCACCACTCCACAGCGGGAGATTTGTGTGTTATAATGTCTTATTGTGCGTGGAAATCACCAAAATCAACACAG
TTTCTTGAGATAACACAGACTATGCAGTACACGTTTGAGACAGAGGAAGGTAAGATGGTTTTTAACAATCACAACAGGCT
CCTTACGGAAACGGACTACTGCATATCGGGAAAGACCGGCTTTACCGCAAAGGCAGGTTACTGCTATGTCGCAGCAGTTG
AAAAGGATGGAAGGAGAATGTGCCTTTCTCTCTTGGGCTGTGGGTGGCCAAACAATAAAAACTATAAGTGGGCTGATGCA
AAGAGTCTGGTGGAGTATGCGGTTTCAGATGCTGCCGAAGATTCTTACTGTGATGCTGCTTGTGTAACAACACAACAGAC
CGGTGATACACGAAACACAATTAACTTAAAATATATAGAAAATAACAAGAAAAATAAGAAAATTTGCAAGAATTTTCTCA
AGAATTTTACAATTAATATAGGAAATTTTTTCTAA

Upstream 100 bases:

>100_bases
GGTTGAGGAGTTTAAGCAGGAGGCAGAGGAAGAGATGCACGTGAAGCTTGATGTGTAGTGCAGGTGTATTGCGTAAGCTG
CCATTTCATATAATAAGGAC

Downstream 100 bases:

>100_bases
ACAAAAATTAAAGTTAAAAATTTAACAAATTAGTTGAAAAAAATTTAGAAATAGTCTATTATCATACTAGCGAAAAATTT
TATTTTTTTATAGATGGAGG

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MQIIIYITDGGNYMVIFLKKYKSKIALMVFICVLVCTVSCLLDFKTLEKQKMPGLEKKSVFMAAENTVAKNTDKAVNEPR
LHAVSAALYDADDETFIYGRNMRDSMANASTTKLLTCIVALEKSDINDTVTISQNAASQPAVKLGLVAGNSYNMKDLLYG
MMLESFNDCAYAIAEHVGGSTEGFAKLMNEKANEIGCLGTYFITPNGLDAENDISFHHSTAGDLCVIMSYCAWKSPKSTQ
FLEITQTMQYTFETEEGKMVFNNHNRLLTETDYCISGKTGFTAKAGYCYVAAVEKDGRRMCLSLLGCGWPNNKNYKWADA
KSLVEYAVSDAAEDSYCDAACVTTQQTGDTRNTINLKYIENNKKNKKICKNFLKNFTINIGNFF

Sequences:

>Translated_384_residues
MQIIIYITDGGNYMVIFLKKYKSKIALMVFICVLVCTVSCLLDFKTLEKQKMPGLEKKSVFMAAENTVAKNTDKAVNEPR
LHAVSAALYDADDETFIYGRNMRDSMANASTTKLLTCIVALEKSDINDTVTISQNAASQPAVKLGLVAGNSYNMKDLLYG
MMLESFNDCAYAIAEHVGGSTEGFAKLMNEKANEIGCLGTYFITPNGLDAENDISFHHSTAGDLCVIMSYCAWKSPKSTQ
FLEITQTMQYTFETEEGKMVFNNHNRLLTETDYCISGKTGFTAKAGYCYVAAVEKDGRRMCLSLLGCGWPNNKNYKWADA
KSLVEYAVSDAAEDSYCDAACVTTQQTGDTRNTINLKYIENNKKNKKICKNFLKNFTINIGNFF
>Mature_384_residues
MQIIIYITDGGNYMVIFLKKYKSKIALMVFICVLVCTVSCLLDFKTLEKQKMPGLEKKSVFMAAENTVAKNTDKAVNEPR
LHAVSAALYDADDETFIYGRNMRDSMANASTTKLLTCIVALEKSDINDTVTISQNAASQPAVKLGLVAGNSYNMKDLLYG
MMLESFNDCAYAIAEHVGGSTEGFAKLMNEKANEIGCLGTYFITPNGLDAENDISFHHSTAGDLCVIMSYCAWKSPKSTQ
FLEITQTMQYTFETEEGKMVFNNHNRLLTETDYCISGKTGFTAKAGYCYVAAVEKDGRRMCLSLLGCGWPNNKNYKWADA
KSLVEYAVSDAAEDSYCDAACVTTQQTGDTRNTINLKYIENNKKNKKICKNFLKNFTINIGNFF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=266, Percent_Identity=29.6992481203008, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082022, Length=260, Percent_Identity=28.0769230769231, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082054, Length=218, Percent_Identity=30.2752293577982, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786851, Length=256, Percent_Identity=28.515625, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42655; Mature: 42655

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIIIYITDGGNYMVIFLKKYKSKIALMVFICVLVCTVSCLLDFKTLEKQKMPGLEKKSV
CEEEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEE
FMAAENTVAKNTDKAVNEPRLHAVSAALYDADDETFIYGRNMRDSMANASTTKLLTCIVA
EEEECCCHHCCCHHHCCCCHHHEEEHHEECCCCCEEEEECCCHHHHCCCHHHHHHHHHHH
LEKSDINDTVTISQNAASQPAVKLGLVAGNSYNMKDLLYGMMLESFNDCAYAIAEHVGGS
HHCCCCCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TEGFAKLMNEKANEIGCLGTYFITPNGLDAENDISFHHSTAGDLCVIMSYCAWKSPKSTQ
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCEECCCCCHHHHHHHHHHCCCCCCCC
FLEITQTMQYTFETEEGKMVFNNHNRLLTETDYCISGKTGFTAKAGYCYVAAVEKDGRRM
EEEEEEHEEEEEECCCCEEEEECCCEEEEECCEEECCCCCCCCCCCEEEEEEECCCHHHH
CLSLLGCGWPNNKNYKWADAKSLVEYAVSDAAEDSYCDAACVTTQQTGDTRNTINLKYIE
HHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEEEEE
NNKKNKKICKNFLKNFTINIGNFF
CCCCCHHHHHHHHHHEEEECCCCC
>Mature Secondary Structure
MQIIIYITDGGNYMVIFLKKYKSKIALMVFICVLVCTVSCLLDFKTLEKQKMPGLEKKSV
CEEEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEE
FMAAENTVAKNTDKAVNEPRLHAVSAALYDADDETFIYGRNMRDSMANASTTKLLTCIVA
EEEECCCHHCCCHHHCCCCHHHEEEHHEECCCCCEEEEECCCHHHHCCCHHHHHHHHHHH
LEKSDINDTVTISQNAASQPAVKLGLVAGNSYNMKDLLYGMMLESFNDCAYAIAEHVGGS
HHCCCCCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TEGFAKLMNEKANEIGCLGTYFITPNGLDAENDISFHHSTAGDLCVIMSYCAWKSPKSTQ
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCEECCCCCHHHHHHHHHHCCCCCCCC
FLEITQTMQYTFETEEGKMVFNNHNRLLTETDYCISGKTGFTAKAGYCYVAAVEKDGRRM
EEEEEEHEEEEEECCCCEEEEECCCEEEEECCEEECCCCCCCCCCCEEEEEEECCCHHHH
CLSLLGCGWPNNKNYKWADAKSLVEYAVSDAAEDSYCDAACVTTQQTGDTRNTINLKYIE
HHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEEEEE
NNKKNKKICKNFLKNFTINIGNFF
CCCCCHHHHHHHHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]