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The map label for this gene is purB [H]

Identifier: 238923900

GI number: 238923900

Start: 1413538

End: 1414989

Strand: Direct

Name: purB [H]

Synonym: EUBREC_1528

Alternate gene names: 238923900

Gene position: 1413538-1414989 (Clockwise)

Preceding gene: 238923899

Following gene: 238923908

Centisome position: 40.98

GC content: 44.28

Gene sequence:

>1452_bases
ATGAGTACAGACAGATACACAAGTCCTCTTTCTGAGCGCTATGCCAGCAAAGAGATGCAGTATATTTTTTCGCAGGATAT
GAAATTTACCACTTGGAGAAAGCTCTGGATTGCTCTTGCAGAGACTGAGATGGAGCTTGGGCTTTCACAGGATGGCAAGC
CGGTTATCACACAGGAACAGATTGATGAATTAAAGGCACATGTGGATGACATCAATTATGATGTGGCAAAGGAGCGTGAG
AAGCTTGTGCGTCACGATGTAATGAGCCATGTCTATGCATATGGACAGCAGTGCCCTAAGGCAGCAGGAATTATACATCT
TGGAGCAACCTCATGCTATGTAGGTGACAATACGGATATCATCATCATGAATGAAGCATTAAAGCTCGTTCACAAAAAGC
TTATAAGTGTTATTGCAGAGCTTGCAAACTTTGCTGATAAGTATAAGAATCAGCCAACTCTTGCATTTACACATTTCCAG
CCGGCACAGCCGACAACAGTTGGAAAGCGTGCCACACTGTGGCTAAATGAGTTTATGCTGGATCTCGAGGATTTAGAGTA
TGTGCAAGGCACATTAAAGCTCCTTGGCTCAAAGGGCACTACAGGAACACAGGCCAGCTTCCTTGAGCTCTTCAACGGAG
ACGATGAGACAATAGACAAGATTGATCCGATGATCGCAAAGAAGATGGGCTTTAAGGATTGTTATCCGGTGTCAGGCCAG
ACATATTCCCGTAAGGTAGATACAAGAGTGGTAAATGTGCTCGCAGGCATTGCTGCATCGGCTACAAAGATGTCAAACGA
TATCAGACTGCTCCAGCATCTTAAGGAAGTAGAGGAGCCGTTTGAGAAGAATCAGATAGGTTCATCAGCCATGGCATACA
AGAGAAATCCTATGAGAAGTGAGAGAATTGCATCTCTTTCAAGATATGTTCTTGCAGATGTAATGAATCCTGCAATTACT
TCAGCTACACAATGGTTTGAGAGAACACTTGACGATTCAGCAAACAAGCGTCTTTCAATACCTGAGGGCTTCCTTGCAAT
AGACGGAATCCTTGACCTCTGCCTCAATGTGGTGGATGGATTGGTAGTATATCCAAAGGTTATTGAAAAGCACTTTATGG
CAGAGATACCATTCATGGCAACAGAGAACATCATGATGGATGCTGTAAAGAACGGCGGAAATCGTCAGGAGCTCCATGAG
AAGATCAGACAGCTCTCAATGCAGGCCGGAAAGACAGTCAAGGAAGAGGGAAAAGAAAATAACCTCGTAGACTTAATCGC
TGCAGATCCTGCATTCGGACTGACAAAGGCTGATATCGAAAAGAACCTTAAGCCTGAGCTTTATGTGGGACGTGCACCAC
GCCAGGTAGAGGTATATCTGCGTGATGTAGTTAATCCGGTGCTTGAAGCACATAAGGACGAGAGTGGAGTAAAAGCAGAG
ATTAACGTATAA

Upstream 100 bases:

>100_bases
TATCCGATGGAGCCACCGAAGGATGATATCAGAGGCGAGTATTTTGATAAAGAAATAGACCTCGAGAGAAAAATGCTTAA
TCGCTAATAAAGGAGAGAAT

Downstream 100 bases:

>100_bases
TATCGAAGTATAGATAACGGTAATTTGCTTGTTTTAACATATTTATCTAAATGAAAAACCCCGGCATATAAACCTGTTTC
ATGGTTTATATGCCGGGGTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSTDRYTSPLSERYASKEMQYIFSQDMKFTTWRKLWIALAETEMELGLSQDGKPVITQEQIDELKAHVDDINYDVAKERE
KLVRHDVMSHVYAYGQQCPKAAGIIHLGATSCYVGDNTDIIIMNEALKLVHKKLISVIAELANFADKYKNQPTLAFTHFQ
PAQPTTVGKRATLWLNEFMLDLEDLEYVQGTLKLLGSKGTTGTQASFLELFNGDDETIDKIDPMIAKKMGFKDCYPVSGQ
TYSRKVDTRVVNVLAGIAASATKMSNDIRLLQHLKEVEEPFEKNQIGSSAMAYKRNPMRSERIASLSRYVLADVMNPAIT
SATQWFERTLDDSANKRLSIPEGFLAIDGILDLCLNVVDGLVVYPKVIEKHFMAEIPFMATENIMMDAVKNGGNRQELHE
KIRQLSMQAGKTVKEEGKENNLVDLIAADPAFGLTKADIEKNLKPELYVGRAPRQVEVYLRDVVNPVLEAHKDESGVKAE
INV

Sequences:

>Translated_483_residues
MSTDRYTSPLSERYASKEMQYIFSQDMKFTTWRKLWIALAETEMELGLSQDGKPVITQEQIDELKAHVDDINYDVAKERE
KLVRHDVMSHVYAYGQQCPKAAGIIHLGATSCYVGDNTDIIIMNEALKLVHKKLISVIAELANFADKYKNQPTLAFTHFQ
PAQPTTVGKRATLWLNEFMLDLEDLEYVQGTLKLLGSKGTTGTQASFLELFNGDDETIDKIDPMIAKKMGFKDCYPVSGQ
TYSRKVDTRVVNVLAGIAASATKMSNDIRLLQHLKEVEEPFEKNQIGSSAMAYKRNPMRSERIASLSRYVLADVMNPAIT
SATQWFERTLDDSANKRLSIPEGFLAIDGILDLCLNVVDGLVVYPKVIEKHFMAEIPFMATENIMMDAVKNGGNRQELHE
KIRQLSMQAGKTVKEEGKENNLVDLIAADPAFGLTKADIEKNLKPELYVGRAPRQVEVYLRDVVNPVLEAHKDESGVKAE
INV
>Mature_482_residues
STDRYTSPLSERYASKEMQYIFSQDMKFTTWRKLWIALAETEMELGLSQDGKPVITQEQIDELKAHVDDINYDVAKEREK
LVRHDVMSHVYAYGQQCPKAAGIIHLGATSCYVGDNTDIIIMNEALKLVHKKLISVIAELANFADKYKNQPTLAFTHFQP
AQPTTVGKRATLWLNEFMLDLEDLEYVQGTLKLLGSKGTTGTQASFLELFNGDDETIDKIDPMIAKKMGFKDCYPVSGQT
YSRKVDTRVVNVLAGIAASATKMSNDIRLLQHLKEVEEPFEKNQIGSSAMAYKRNPMRSERIASLSRYVLADVMNPAITS
ATQWFERTLDDSANKRLSIPEGFLAIDGILDLCLNVVDGLVVYPKVIEKHFMAEIPFMATENIMMDAVKNGGNRQELHEK
IRQLSMQAGKTVKEEGKENNLVDLIAADPAFGLTKADIEKNLKPELYVGRAPRQVEVYLRDVVNPVLEAHKDESGVKAEI
NV

Specific function: Unknown

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=480, Percent_Identity=54.5833333333333, Blast_Score=550, Evalue=1e-156,
Organism=Homo sapiens, GI183227688, Length=395, Percent_Identity=57.9746835443038, Blast_Score=488, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17508577, Length=486, Percent_Identity=38.6831275720165, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI32564234, Length=351, Percent_Identity=44.1595441595442, Blast_Score=307, Evalue=7e-84,
Organism=Saccharomyces cerevisiae, GI6323391, Length=468, Percent_Identity=52.7777777777778, Blast_Score=512, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24647570, Length=480, Percent_Identity=52.7083333333333, Blast_Score=506, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 54264; Mature: 54133

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDRYTSPLSERYASKEMQYIFSQDMKFTTWRKLWIALAETEMELGLSQDGKPVITQEQ
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
IDELKAHVDDINYDVAKEREKLVRHDVMSHVYAYGQQCPKAAGIIHLGATSCYVGDNTDI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEECCCEEEECCCCCE
IIMNEALKLVHKKLISVIAELANFADKYKNQPTLAFTHFQPAQPTTVGKRATLWLNEFML
EEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
DLEDLEYVQGTLKLLGSKGTTGTQASFLELFNGDDETIDKIDPMIAKKMGFKDCYPVSGQ
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
TYSRKVDTRVVNVLAGIAASATKMSNDIRLLQHLKEVEEPFEKNQIGSSAMAYKRNPMRS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHH
ERIASLSRYVLADVMNPAITSATQWFERTLDDSANKRLSIPEGFLAIDGILDLCLNVVDG
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LVVYPKVIEKHFMAEIPFMATENIMMDAVKNGGNRQELHEKIRQLSMQAGKTVKEEGKEN
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHCCCCC
NLVDLIAADPAFGLTKADIEKNLKPELYVGRAPRQVEVYLRDVVNPVLEAHKDESGVKAE
CEEEEEECCCCCCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEE
INV
ECC
>Mature Secondary Structure 
STDRYTSPLSERYASKEMQYIFSQDMKFTTWRKLWIALAETEMELGLSQDGKPVITQEQ
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
IDELKAHVDDINYDVAKEREKLVRHDVMSHVYAYGQQCPKAAGIIHLGATSCYVGDNTDI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEECCCEEEECCCCCE
IIMNEALKLVHKKLISVIAELANFADKYKNQPTLAFTHFQPAQPTTVGKRATLWLNEFML
EEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
DLEDLEYVQGTLKLLGSKGTTGTQASFLELFNGDDETIDKIDPMIAKKMGFKDCYPVSGQ
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
TYSRKVDTRVVNVLAGIAASATKMSNDIRLLQHLKEVEEPFEKNQIGSSAMAYKRNPMRS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHH
ERIASLSRYVLADVMNPAITSATQWFERTLDDSANKRLSIPEGFLAIDGILDLCLNVVDG
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LVVYPKVIEKHFMAEIPFMATENIMMDAVKNGGNRQELHEKIRQLSMQAGKTVKEEGKEN
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHCCCCC
NLVDLIAADPAFGLTKADIEKNLKPELYVGRAPRQVEVYLRDVVNPVLEAHKDESGVKAE
CEEEEEECCCCCCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEE
INV
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]