Click here to switch to the map view.

The map label for this gene is purF [H]

Identifier: 238923899

GI number: 238923899

Start: 1411962

End: 1413524

Strand: Direct

Name: purF [H]

Synonym: EUBREC_1527

Alternate gene names: 238923899

Gene position: 1411962-1413524 (Clockwise)

Preceding gene: 238923898

Following gene: 238923900

Centisome position: 40.93

GC content: 45.04

Gene sequence:

>1563_bases
ATGAACCAGGACAATAACATAAATCAGTATATAAATACTAGTATAGATACAAAAAGTCATACCGGTACAAAATTAAAAAG
GTGTTCTGATATTGACGAGCATAACCATGTATTTGATGAGCTTCATGAGGAGTGTGGTGTGTTCGGCATGTATGATTTTG
ATGGAGGAAATGTCGCATCCACTATCTATTATGGCCTTTTTGCGCTTCAGCACAGAGGACAGGAAAGCTGTGGTATTGCA
GTGAGTGATACACACGGACCAAAGGGAAAGGTGACCACACACAAGGGTATGGGCCTTGTAAATGAGGTATTTACTCCTGA
TATTTTAGAGCCTATGAAGGGTGATATCGGAGTGGGACATGTGCGCTATTCAACAGCAGGCTCATCAACACGTGAGAATG
CACAGCCTCTCGTATTAAATTATGTCAAGGGCACACTTGCCATGGCACACAACGGAAACCTCATTAACGCAAAGGAGCTT
CGCAAGGAGCTTGAGTATACAGGAGCAATTTTCCAGACCACAATCGACTCTGAGGTTATAGCTTATCATATCGCCAGAGA
GCGTCTTAACTCAAAAACAGCGGAGGAGGCGGTCAGACGTGCCTGCCAGAAGCTTAAGGGAGCCTATGCACTTGTGGTTG
AGAGCCCGAGAAAGCTTATTGCAGCCAGAGACCCATTTGGTTTTAAGCCTCTTTGCATTGGAAAGAGAGACAATGCATAT
ATCGTTACATCTGAGACCTGTGCGCTTGATACAATCGGTGCAGAGTTTGTCAGGGATATAGAGCCGGGAGAGGTTATCAC
CATCACTCCTGAAAAGGGCATTGAGTCTGATATGACTATGGCACTTGCCCCTGAAAAACAGGCCAGATGCGTGTTTGAAT
ACATTTATTTCGCAAGACCTGACAGCCATATTGACGGAGTAAGCGTGTATAGCAGCCGTATCAAGGCTGGCAGATTCCTT
GCAATGGATTCACCGGTTGAGGCTGATATCGTGACAGGAGTACCGGAGTCAGGAAATGCCGCTGCTCTTGGTTATTCACT
TGAGTCGGGCATTCCATATGGCACAGCATTTGTCAAGAACGGTTATGTCGGCAGGACATTTATCAAGCCTAAGCAGTCAA
GCCGTGAATCAAGCGTGCAGATAAAGCTCAATGTATTAAAGGAGGCTGTTAAGGGCAAGAGAGTTGTTATGATTGATGAT
TCAATTGTGCGAGGAACAACCTCAGACAGAATAGTGCGTATGTTAAGAGAGGCGGGTGCCACAGAGGTGCATGTGCGCAT
CAGTTCACCGCCATTCTTATGGCCTTGCTATTTCGGTACAGATATTCCTGCAAGAGAGCAGCTTATCGCTTACAACAGGA
CTATTGAGGATATCAGACAGATTATAGGTGCAGATTCTTTAGGCTACCTTGGAATTGACAGACTTCATGAGATGGTAGAG
GGATTGCCAATCTGCATGGGCTGTTTCACAGGAAAATATCCGATGGAGCCACCGAAGGATGATATCAGAGGCGAGTATTT
TGATAAAGAAATAGACCTCGAGAGAAAAATGCTTAATCGCTAA

Upstream 100 bases:

>100_bases
AAATATAAAGAGGCATTTGAGCTTCTTACAGGAAGCAAATTCAGCCGCTAATATATTATTTATTCCGCGTTACTATTTCG
CAATTATAAACGGAGATATT

Downstream 100 bases:

>100_bases
TAAAGGAGAGAATATGAGTACAGACAGATACACAAGTCCTCTTTCTGAGCGCTATGCCAGCAAAGAGATGCAGTATATTT
TTTCGCAGGATATGAAATTT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MNQDNNINQYINTSIDTKSHTGTKLKRCSDIDEHNHVFDELHEECGVFGMYDFDGGNVASTIYYGLFALQHRGQESCGIA
VSDTHGPKGKVTTHKGMGLVNEVFTPDILEPMKGDIGVGHVRYSTAGSSTRENAQPLVLNYVKGTLAMAHNGNLINAKEL
RKELEYTGAIFQTTIDSEVIAYHIARERLNSKTAEEAVRRACQKLKGAYALVVESPRKLIAARDPFGFKPLCIGKRDNAY
IVTSETCALDTIGAEFVRDIEPGEVITITPEKGIESDMTMALAPEKQARCVFEYIYFARPDSHIDGVSVYSSRIKAGRFL
AMDSPVEADIVTGVPESGNAAALGYSLESGIPYGTAFVKNGYVGRTFIKPKQSSRESSVQIKLNVLKEAVKGKRVVMIDD
SIVRGTTSDRIVRMLREAGATEVHVRISSPPFLWPCYFGTDIPAREQLIAYNRTIEDIRQIIGADSLGYLGIDRLHEMVE
GLPICMGCFTGKYPMEPPKDDIRGEYFDKEIDLERKMLNR

Sequences:

>Translated_520_residues
MNQDNNINQYINTSIDTKSHTGTKLKRCSDIDEHNHVFDELHEECGVFGMYDFDGGNVASTIYYGLFALQHRGQESCGIA
VSDTHGPKGKVTTHKGMGLVNEVFTPDILEPMKGDIGVGHVRYSTAGSSTRENAQPLVLNYVKGTLAMAHNGNLINAKEL
RKELEYTGAIFQTTIDSEVIAYHIARERLNSKTAEEAVRRACQKLKGAYALVVESPRKLIAARDPFGFKPLCIGKRDNAY
IVTSETCALDTIGAEFVRDIEPGEVITITPEKGIESDMTMALAPEKQARCVFEYIYFARPDSHIDGVSVYSSRIKAGRFL
AMDSPVEADIVTGVPESGNAAALGYSLESGIPYGTAFVKNGYVGRTFIKPKQSSRESSVQIKLNVLKEAVKGKRVVMIDD
SIVRGTTSDRIVRMLREAGATEVHVRISSPPFLWPCYFGTDIPAREQLIAYNRTIEDIRQIIGADSLGYLGIDRLHEMVE
GLPICMGCFTGKYPMEPPKDDIRGEYFDKEIDLERKMLNR
>Mature_520_residues
MNQDNNINQYINTSIDTKSHTGTKLKRCSDIDEHNHVFDELHEECGVFGMYDFDGGNVASTIYYGLFALQHRGQESCGIA
VSDTHGPKGKVTTHKGMGLVNEVFTPDILEPMKGDIGVGHVRYSTAGSSTRENAQPLVLNYVKGTLAMAHNGNLINAKEL
RKELEYTGAIFQTTIDSEVIAYHIARERLNSKTAEEAVRRACQKLKGAYALVVESPRKLIAARDPFGFKPLCIGKRDNAY
IVTSETCALDTIGAEFVRDIEPGEVITITPEKGIESDMTMALAPEKQARCVFEYIYFARPDSHIDGVSVYSSRIKAGRFL
AMDSPVEADIVTGVPESGNAAALGYSLESGIPYGTAFVKNGYVGRTFIKPKQSSRESSVQIKLNVLKEAVKGKRVVMIDD
SIVRGTTSDRIVRMLREAGATEVHVRISSPPFLWPCYFGTDIPAREQLIAYNRTIEDIRQIIGADSLGYLGIDRLHEMVE
GLPICMGCFTGKYPMEPPKDDIRGEYFDKEIDLERKMLNR

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=509, Percent_Identity=38.8998035363458, Blast_Score=353, Evalue=3e-97,
Organism=Homo sapiens, GI4826742, Length=235, Percent_Identity=28.936170212766, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788651, Length=457, Percent_Identity=40.9190371991247, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1790167, Length=228, Percent_Identity=32.4561403508772, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17554892, Length=483, Percent_Identity=42.2360248447205, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17539970, Length=221, Percent_Identity=28.0542986425339, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17532899, Length=223, Percent_Identity=27.8026905829596, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17532897, Length=223, Percent_Identity=27.8026905829596, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323958, Length=477, Percent_Identity=37.5262054507338, Blast_Score=271, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24659598, Length=489, Percent_Identity=41.9222903885481, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI28573187, Length=495, Percent_Identity=41.4141414141414, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24659604, Length=467, Percent_Identity=42.3982869379015, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI21357745, Length=218, Percent_Identity=27.5229357798165, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 57589; Mature: 57589

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQDNNINQYINTSIDTKSHTGTKLKRCSDIDEHNHVFDELHEECGVFGMYDFDGGNVAS
CCCCCCCHHHCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCEEEEEECCCCCHHH
TIYYGLFALQHRGQESCGIAVSDTHGPKGKVTTHKGMGLVNEVFTPDILEPMKGDIGVGH
HHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEECCCCCHHHHHCCCHHHHHCCCCCCCCE
VRYSTAGSSTRENAQPLVLNYVKGTLAMAHNGNLINAKELRKELEYTGAIFQTTIDSEVI
EEEECCCCCCCCCCCCEEEEEHHHEEEEECCCCEECHHHHHHHHHHCCHHEEEECCHHHH
AYHIARERLNSKTAEEAVRRACQKLKGAYALVVESPRKLIAARDPFGFKPLCIGKRDNAY
HHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCHHHEEECCCCCCCCEEECCCCCEE
IVTSETCALDTIGAEFVRDIEPGEVITITPEKGIESDMTMALAPEKQARCVFEYIYFARP
EEECCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHCCC
DSHIDGVSVYSSRIKAGRFLAMDSPVEADIVTGVPESGNAAALGYSLESGIPYGTAFVKN
CCCCCCHHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCEEEEEEEHHCCCCCCCHHHCC
GYVGRTFIKPKQSSRESSVQIKLNVLKEAVKGKRVVMIDDSIVRGTTSDRIVRMLREAGA
CCCCEEEECCCCCCCCCCEEEEHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCC
TEVHVRISSPPFLWPCYFGTDIPAREQLIAYNRTIEDIRQIIGADSLGYLGIDRLHEMVE
CEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHC
GLPICMGCFTGKYPMEPPKDDIRGEYFDKEIDLERKMLNR
CCCEEHHHCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNQDNNINQYINTSIDTKSHTGTKLKRCSDIDEHNHVFDELHEECGVFGMYDFDGGNVAS
CCCCCCCHHHCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCEEEEEECCCCCHHH
TIYYGLFALQHRGQESCGIAVSDTHGPKGKVTTHKGMGLVNEVFTPDILEPMKGDIGVGH
HHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEECCCCCHHHHHCCCHHHHHCCCCCCCCE
VRYSTAGSSTRENAQPLVLNYVKGTLAMAHNGNLINAKELRKELEYTGAIFQTTIDSEVI
EEEECCCCCCCCCCCCEEEEEHHHEEEEECCCCEECHHHHHHHHHHCCHHEEEECCHHHH
AYHIARERLNSKTAEEAVRRACQKLKGAYALVVESPRKLIAARDPFGFKPLCIGKRDNAY
HHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCHHHEEECCCCCCCCEEECCCCCEE
IVTSETCALDTIGAEFVRDIEPGEVITITPEKGIESDMTMALAPEKQARCVFEYIYFARP
EEECCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHCCC
DSHIDGVSVYSSRIKAGRFLAMDSPVEADIVTGVPESGNAAALGYSLESGIPYGTAFVKN
CCCCCCHHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCEEEEEEEHHCCCCCCCHHHCC
GYVGRTFIKPKQSSRESSVQIKLNVLKEAVKGKRVVMIDDSIVRGTTSDRIVRMLREAGA
CCCCEEEECCCCCCCCCCEEEEHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCC
TEVHVRISSPPFLWPCYFGTDIPAREQLIAYNRTIEDIRQIIGADSLGYLGIDRLHEMVE
CEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHC
GLPICMGCFTGKYPMEPPKDDIRGEYFDKEIDLERKMLNR
CCCEEHHHCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]